Serum Protein Values of Racehorse in Korea

이창우,이성엽,이승모,엄영호,Lee Chang-Woo,Lee Sung-Yub,Lee Sung-Mo,Eom Yeong-Ho The Korean Society of Veterinary Clinics 1987 한국임상수의학회지 Vol.4 No.2

임상적으로 건강하고 휴식상태에 있는 한국 경주마의 혈청단백질치를 조사하였다. 총단백질농도는 6.5$\pm$0.3g/100ml(평균$\pm$표준편차)이였다. 알부민 농도는 3.4$\pm$0.2, $\alpha$-글로부린 농도는 0.8$\pm$0.1, $\beta$-글로부린 농도는 1.2$\pm$0.2, ${\gamma}$-글로부린 농도는 1.1$\pm$0.3g/100ml이었으며, A/G 비는 1.15$\pm$0.21이었다. 성, 연령, 계절에 따르는 혈청단백질치의 차이를 통계적으로 분석하여 보고하였다.

소의 혈청 Ornithine Carbamyltransferase 활성도 측정에 필요한 적합한 조건에 관한 연구

이창우,Lee Chang-Woo 한국임상수의학회 1985 한국임상수의학회지 Vol.2 No.1

The optimal conditions for the evaluation of serum ornithine carbamyltransferase activity, based on the do-termination of citrulline formed during the enzymatic reaction, were investigated and the serum ornithine carbamyltransferase activity of cattle were surveyed. Barbital-acetate buffer(70m moles/L, pH 7.0 at $37^{\circ}C$) were usea for the entire experiment. The results were as follows. 1. When the concentration of $H_2SO_4$ in color reagent exceeds 3.0 m1/100m1 the serum protein precipitated and absorbance increased. 2. The concentrations of antipyrine and diacetylmonoxime required for maximal color formation were 1g/L and 5g/L, respectively. 3. The absorbance was maximal when the reaction mixture was boiled for 25 minutes. 4. The chromogen were stable for at least 60 minutes under loon lighting condition, but decolorized rapidly under direct sunlight. 5. The minimal concentration of urease solution(Sigma Chemical Co., Type III) required for elimination of serum urea was 0, 6mg/ml. 6. When the concentration of L-ornithine solution increased up to 22m moles/L, the ornithine carbamyltransferase activity was not inhibited by zwitterion of ornithine. 7. In accordance with the increase of carbamylphosphate concentration the ornithine carbamyltransferase activity increased and the nonenzymatic citrulline production also increased slightly. 8. The standard curve of citrulline revealed linear pattern within the range of this experiment (0.1~4.0m moles/L). 9. The ornithine carbamyltransferase activities of normal cattle investigated in this laboratory were 6.85$\pm$4.38U/L (mean$\pm$SD) in cows and 2.89$\pm$2.50U/L in bulls. The range of the activities were 0.39~29.12U/L in cows and 0.06~17.34U/L in bulls.

사슴의 출혈성패혈증예

이창우,Lee Chang Woo 대한수의사회 1978 대한수의사회지 Vol.14 No.2

An outbreak of hemorrhagic septicemia was reported in a herd of 25 Formosan deer and 27 elks, 3 of Formosan deer and 4 of elks died of it. Clinically they were characterized by dullness, anorexia, loss of rumination, bloody and mucous soft feces, and sudd

인간이 갖고있는 결함과 한계에 맞는 방재체제가 중요

이창우,Lee, Chang-U 한국화재보험협회 1989 防災와 保險 Vol.40 No.-

이공적 사고

이창우,Lee, Chang-U 한국과학기술단체총연합회 1986 과학과 기술 Vol.19 No.2

반추동물 신증의 조기진단을 위한 뇨효소 측정법 1. 효소활성도 측정을 위한 적합한 조건과 정상범위에 관하여

이창우,이경갑,Lee Chang-Woo,Lee Kyoung-Kap 한국임상수의학회 1989 한국임상수의학회지 Vol.6 No.2

Present experiment was performed to establish the optimal reaction conditions for measurement of urinary gamma-glutamyltranspeptidase(${\gamma}$-GTP), N-acetyl-${\beta}$-D-glucosaminidase (AGS) and alanine aminopeptidase(AAP) activities in bovine and to investigate in vitro stability of the enzymes, within-run imprecision of the methods, and normal ranges. 1. The optimal wavelength for measurement of ${\gamma}$-GTP activity was 545nm. 2. The optimal pH of Tris-HCI buffer containing glycylglycine for measurement of urinary ${\gamma}$-GTP activity was 7.6~7.8(37$^{\circ}C$). 3. Coefficient of variance for within-run imprecision of urinary ${\gamma}$-GTP activity ranged from 4.8 to 7.2% and there was no significant difference among replications, 4. The optimal wavelength for measurement of urinary AGS activity was 405nm. 5. The optimal pH of citrate buffer for measurement urinary of AGS activity was 4.0(37$^{\circ}C$). 6. Coefficient of variance for within-run imprecision of urinary AGS activity ranged from 3.9 to 6.1% and there was no significant difference among replications. 7. The optimal wavelength for measurement of urinary AAP activity was 400nm. 8. The optimal pH of phosphate buffer for measurement of urinary AAP was 7.8. 9. Coefficient of variance for within-run imprecision of urinary AAP activity ranged from 2.5 to 4.8% and there was no significant difference among replications. 10. ${\gamma}$-GTP and AGS activities were increased significantly by gel-filtration. 11. Turbidity interfered with measurement of urinary AAP activity in bovine unless the specimen was gel-filterated. 12. Preservation of the specimen at 5$^{\circ}C$ or -20$^{\circ}C$ did not affect the AGS activity at least for 7 days after collection. 13. Preservation of the specimen at 5$^{\circ}C$ or 20$^{\circ}C$ did not affect the ${\gamma}$-GTP and AAP activities statistically, but some individual specimens revealed fluctuation during preservation. 14. ${\gamma}$-GTP, AGS and AAP activities revealed fluctuation by the tine of the day when the specimen was collected. 15. The normal ranges of urinary ${\gamma}$ -GTP, AGS and AAP activities were 6.60${\pm}$3.26(2.36-14.50), 1.31 ${\pm}$ 0.81(0.33-3.78), and 1.73 ${\pm}$ 0.55(0.77-3.03)U/l. respectively.

Cytochemical Characteristics of Blood and Bone Marrow Cells in Dog

이창우,하세가와 아쯔히꼬,오노 겐이찌로,고이즈까 료,양만표,Lee Chang Woo,Hasegawa A.,Ono K.,Goitsuka R.,Yang M.P. The Korean Society of Veterinary Clinics 1990 한국임상수의학회지 Vol.7 No.1

The cytochemical characteristics of the hematopoietic cells in blood and bone marrow from 3 clinically healthy dogs were examined using a battery of cytochemical stains. Alkaline phosphotase activity was demonstrated in eosinophilic series and occassionally in progranulocytes. A variety of cells exhibited acid phosphatase activity, but tartrate-resistant acid phosphatase activity was seen only in eosinophilic series. Peroxidase activity was observed in myeloblasts to segmented cells of granulocytic series and in monocytes. ${\alpha}$-naphthyl acetate esterase activity was found in monocytes and occassionally in lymphocytes. Naphthyl-AS-D-chloroacetate esterase marked neutrophilic series from myeloblasts to segmented cells. ${\beta}$-Glucuronidase activity was detected in a variety of cells except the cells of erythrocytic series. Periodic acid Schiff stain-positive granules were demonstrated in the neutrophilic and eosinophilic series from myelocytes to segmented cells and in monocytes and occassionally in lymphocytes. Sudan black B stain-positive granules marked granulocytic series from myeloblasts to segmented tells and monocytes. 건강한 개의 혈액세포와 골수세포의 세포화학적 특성을 조사하였다. 이 실험에서 혈액과 골수 시료에 항응고제를 일체사용하지 않았으며 시료채취후 즉시 도말표본을 작성하여 30분 이내에 반응을 실시하였다. 이 실험의 결과는 아래와 같다. 1. alkaline phosphatase의 활성은 호산구계통과 간혹 전골수구에서 양성반응을 나타내었다. 2. acid phosphatase의 활성은 대부분의 계통의 세포에서 양성반응을 나타내지만 tartrate로 억제하면 호산구계만 양성반응을 나타내었다. 3. peroxidase 활성은 골수구계통의 모든 세포에서 양성반응을 나타내며 단구에서는 미약한 양성의 미세과립을 나타내었다. 4. naphthyl-AS-D-chloroacetate esterase활성은 호중구계 세포에서만 양성을 나타낸다. 5. $\alpha$-naphthyl acetate esterase활성은 단구와 일부의 임파구에서 양성을 나타낸다 6. Sudan black B 염색은 골수구계 세포와 단구계 세포에서 양성을 나타내었다. 7. $\beta$-glucuronidase 활성은 적혈구계를 제외한 모든 세포에서 양성반응을 나타내었다.

암모니아 펄스 플라즈마를 이용한 원자층 증착된 질화텅스텐 확산방지막 특성

이창우,Lee, Chang-Woo 한국마이크로전자및패키징학회 2004 마이크로전자 및 패키징학회지 Vol.11 No.4

We have deposited the W-N diffusion barrier on Si substrate with $NH_3$ pulse plasma enhanced atomic layer deposition (PPALD) method by using $WF_6$ and $NH_3$ gases. The $WF_6$ gas reacts with Si that the surface corrosion occurs severely, but the $NH_3$ gas incorporated with pulse plasma and $WF_6$ gas are easily deposited W-N thin film without Si surface corrosion. Because the $NH_3$ with pulse plasma can be active species dissociated and chemisorbed on Si. Thus the Si surface are covered and saturated with nitrogen, which are able to deposit the W-N thin film. We also examine the deposition mechanism and the effect of $NH_3$ pulse plasma treatment. 암모니아 펄스플라즈마를 이용하여 $WF_6$ 가스와 $NH_3$ 가스를 교대로 흘려줌으로써 Si 기판위에 질화텅스텐 확산방지막을 증착하였다. $WF_6$ 가스는 Si과 반응하여 표면침식이 과도히 발생하였으나 암모니아 ($NH_3$)가스를 펄스 플라즈마를 인가하여 $WF_6$와 같이 사용하면 Si 표면을 질화처리 함으로써 표면침식을 막아주며 질화텅스텐 박막을 쉽게 증착할 수 있었다. 그 이유는 암모니아 가스의 분해를 통한 Si 기판의 흡착을 용이하게 하여 질화텅스텐 박막 증착이 가능하기 때문이다. 이러한 증착 미케니즘과 암모니아 펄스 플라즈마 효과에 대하여 조사하였다.

국내 도로터널내 차량항력계수 관련 연구

이창우,이경복,Lee, Chang-Woo,Lee, Kyeong-Bok 한국터널지하공간학회 2005 터널기술 Vol.7 No.4

터널환기력 중 중요한 비중을 차지하고 있는 교통환기력을 추정하는데 있어서 항력계수는 중요한 설계인자이다. 현재 국내 도로터널 환기시스템 설계시 적용하고 있는 항력계수는 국내 차량특성을 고려하지 않은 외국자료의 인용, 폐색율 만에 기초하며 슬립스트리밍효과를 고려하지 않고 있는 문제를 가지고 있다. 본 논문에서는 국내 터널내 교통환기력의 정확한 추정에 목표를 두고 (1) 현재 운행 중인 국내차량 특성을 고려한 전면 투영면적를 추정하고, (2) 도로서비스 수준별 차량배치상황을 CFD분석하여 슬립스트림밍 효과를 분석하여 차량 1대당 저항계수인 $K_{blockage}$와 항력계수를 분석하였다. Drag coefficient is one of the critical design factors to quantify the piston effect in vehicle tunnels. Several problems are raised on the drag coefficient currently applied for the ventilation system design; unverified adoption of the projected frontal area of the vehicle from the foreign study in the past, and lack of consideration for the slip-streaming effect. This study aims at better estimation of the traffic-induced ventilation force in the local tunnels. Values for the projected frontal area of the vehicles running on the local roads at present are proposed and results of an extensive CFD study are studied on the slip-streaming effects in various traffic conditions to quantify $K_{blockage}$ and the drag coefficient in the domestic tunnels.

백서 복근에 매식된 동결 건조 및 방사선 멸균처리된 동종골의 조직 반응에 관한 실험적 연구

이창우,임창준,이동근,김수남,Lee, Chang-Woo,Yim, Chang-Joon,Lee, Dong-Kuen,Kim, Soo-Nam 대한악안면성형재건외과학회 1991 Maxillofacial Plastic Reconstructive Surgery Vol.13 No.3

In this study, the healing changes of the implanted bone and its surrounding tissues were examined on the histopathologic basis following implantation of the freeze - dried and radiation - sterilized allogeneic bone in Rectus abdominicus of the rat. This study was performed to see the tissue recations after implantation of the freeze - dried and radiation - sterilized allogeneic bone and whether osteogenesis or osteo - induction or osteo - conduction is happened. And the results were as follows : 1. The shape of the implanted allogeneic bone of the 1, 2 - week group specimen was similar to that of normal bone in light - microscopic finding and the atrophy of cellular organells was found in trans - mission electron - microscopic finding. 2. The implanted allogeneic bone was surrounded with the dense fibroconnective tissues, and infiltration of the chronic inflammatory cells gradually became increased. 3. Hyaline degeneration was observed in the surrounding tissue at the 3, 4, 6 - week group specimen. 4. Light - microscopically the resorption of implanted bone became prominent after 4 - week group and the necrosis of allogeneic bone implant became severe with loss of cell components in lacuna. 5. Electron - microscopically, the osteoclast - like cells ere fond after, 2 - week group. It is summarized that the osteo - conduction potential of the bone is remained just after implanting the freeze - dried and radiation - sterilized allogeneic bone on Rectus abdominicus of the rat, but gradually it disappeared with the gradual increse of chronic inflammatory reaction and osteoclastic activity. So it is suggested that the antigenicity of the freeze - dried and radiation - sterilized bone is remained and it has little osteo - conductive activity when it is implanted in the muscle.