가래나무(Juglans mandshurica) 톱밥의 첨가량이 느타리버섯의 수량에 미치는 영향

이찬중,정종천,전창성,문지원,공원식,서장선,박기춘,신유수,이정훈 한국버섯학회 2013 한국버섯학회지 Vol.11 No.4

This study was carried out to investigated optimum mixing ratio of Korean natural Juglans mandshurica using as functional plants. Total nitrogen and carbon source of J. mandshurica was 0.21% and 46.0%, respectively and C/N ratio was 219. Total nitrogen source and pH of substrate mixed with J. mandshurica was 2.3~2.9 and 5.0, respectively. The contents of CaO, MgO and Na2O at J. mandshurica media were higher at harvest media than inoculation media. Mycelial growth was the fastest at J. mandshurica 20%, and slower by increase of J. mandshurica substrate. Yields of fruiting body show the highest to 163 g/850 mL of medium which are addition 10% of J. mandshurica sawdust, and diameter and thick of pileus were the highest, too. The L value of pileus and stipes were increased by increase of J. mandshurica substrate, but there was no significant difference in the a-value and the b-value. The contents of P2O5 and K2O of fruiting body were increased at J. mandshurica substrate, but there was no significant difference in contents of CaO, MgO and Na2O. The contents of Cu of fruiting body was decreased by increase of J. mandshurica substrate, but Fe, Mn and Zn were increased.

옻나무톱밥 첨가에 따른 느타리버섯의 생육 특성 및 수량성

이찬중,유영미,한주연,정종천,전창성,문지원,공원식,서장선 한국버섯학회 2014 한국버섯학회지 Vol.12 No.3

This study was carried out to investigated optimum mixing ratio of Korean natural Rhus verniciflua for production of functional oyster mushroom. Mycerial growth was some fast at addition of R. verniciflua sawdust, and not significant difference by increase of R. verniciflua substrate. Yields of fruiting body show the highest to 150.2 g/850 ml of medium which are addition 20% of R. verniciflua sawdust, and diameter of pileus and thick of stipes were the highest at addition 10% of R. verniciflua sawdust. The L value of stipes were the highest at addition 10% of R. verniciflua sawdust and the L value of pileus were decreased by increase of R. verniciflua substrate, but there was no significant difference in the a- value and the b-value.

병재배 느타리버섯 ‘흑타리’의 스마트팜 환경조절을 통한 재배사내 생육환경 분석

이찬중,이은지,박혜성,공원식,유병기,이성현 한국버섯학회 2019 버섯 Vol.23 No.1

특용작물 병해충 도감 ② - 양송이 (I)

이찬중,Lee, Chan-Jung 한국작물보호협회 2015 자연과 농업 Vol.310 No.-

양송이버섯 재배시 자주식 배지교반기 활용 배지의 특성 및 수량성

이찬중,유병기,박혜성,이은지,Lee, Chan-Jung,Yu, Byeong-Kee,Park, Hye-sung,Lee, Eun-Ji,Min, Gyeong-Jin 한국버섯학회 2020 한국버섯학회지 Vol.18 No.3

본 실험은 농가 관행인 굴삭기 뒤집기와 자주식교반기를 이용한 뒤집기 비교 시험을 통해 발효단계별 배지의 특성을 조사하였다. 야외발효배지의 온도는 자주식교반기 처리에서 뒤집기 후 온도 상승이 빨라지는 경향을 보였다. 후발효배지의 온도는 농가관행인 굴삭기 처리구에서 온도가 높게 유지되면서 후발효과 진행되었다. 야외발효 단계에서 배지에는 고온성 세균 (Bacillus spp.), 방선균, 형광성 Pseudomonas 속, 사상균 등 다양한 미생물들이 분포하였고, 특히 배지발효 과정에서 배지의 분해와 발열에 관여하는 호기성 세균의 밀도는 자주식교반기 처리구에서 상대적으로 높았고, 고온성 발효과정 중 푸른곰팡이균과 중온성 방선균은 생육이 억제되거나 사멸되는 양상을 보였다. 유기물을 분해하는데 중요한 역할을 하고 있는 고온성 방선균은 자주식교반기 처리에서 농가관행보다 높은 밀도를 보였다. 처리별 볏짚의 길이는 자주식교반기를 사용하였을 경우 조금 짧았고, 수분함량은 처리간에 뚜렷한 차이를 보이지 않았다. pH와 EC는 처리간에 뚜렷한 차이를 보이지 않았고, a, b값은 자주식교반기에 의한 뒤집기가 진행될수록 높아지는 경향을 보였다. 발효 단계별 배지의 CN율을 조사한 결과 농가관행인 굴삭기의 경우 23.1에서 시작하여 5차 뒤집기에서는 16.2까지 낮아 졌고, 자주식교반기의 경우 23.3에서 16.9로 낮아 졌다. 따라서 처리간에는 뚜렷한 차이를 보이지 않았다. 처리별 수확량을 조사한 결과 1주기에서는 자주식교반기 처리에서 20%의 증수효과가 있었고, 2주기에서는 28%, 3주기에서는 26%의 증수효과가 있었으며, 전체적으로 23%의 수량 증대효과가 있었다. This study was conducted to investigate the characteristics of the medium used on the composting step, comparing the excavator agitator with the self-propelled turner. The temperature of the outdoor composting medium tended to increase rapidly after flipping in the turner. The late composting medium temperature was maintained at the excavator treatment area (farm practice), and the late composting effect progressed. During the field composting stage, various microorganisms such as Bacillus spp., Actinomycetes, fluorescent Pseudomonas spp., and filamentous fungi were distributed in the medium, and the density of aerobic bacteria involved in the decomposition of the medium was increased. Under high-temperature composting conditions, blue fungi, and mesophilic actinomycetes were inhibited or killed. Thermophilic actinomycetes, which play an important role in decomposing organic matter, showed higher densities than those observed in farm practices in the self-propelled turner process. The length of rice straw was slightly shorter when the self-propelled turner was used, and the water content did not show any significant difference between treatments. The a and b values tended to increase as the inverter was turned over. The CN ratio of the composting broth was lowered from 23.1 to 16.2 for the 5th turnover in the context of farming practices, and from 23.3 to 16.9 in the context of the self-propelled turner. The yield of each treatment was increased by 20% in 1 period, 28% in 2 periods, and 26% in 3 periods; the overall yield was 23%.

버섯 세균성회색무늬병균(Pseudomonas agarici)에 대한 길항 세균 Alcaligenes sp. HC12의 대량배양을 위한 최적 배양조건

이찬중,문지원,정종천 한국버섯학회 2016 한국버섯학회지 Vol.14 No.4

This study was conducted to investigate optimum conditions for mass production of ntagonistic microbes Alcaligenes sp. HC12. Alcaligenes sp. HC12 had a potent biological control agent to control browning disease caused by Pseudomonas agarici. Alcaligenes sp. HC12 markedly showed the antagonistic activity against Pseudomonas agarici, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Alcaligenes sp. HC12, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 9.0 and 30o, respectively. The optimal concentration of medium elements for the growth of pathogen inhibitor bacterium(Alcaligenes sp. HC12) was determined as follows: 0.5% dextrine, 1.5% yest extract, 1.0% NaNO3, 0.5% KH2PO4, and 1.5% asparagine.

rDNA의 ITS 부위 염기서열 분석에 의한 겨울우산버섯 (Polyporus)속 균주의 유전적인 유연관계 분석

이찬중,전창성,정종천,공원식 한국버섯학회 2012 한국버섯학회지 Vol.10 No.1

This study was carried to identify a correct species and asses genetic diversity within the same species of Polyporus spp. preserved in Division of applied Microbiology. Contaminated isolates showed different growth rates, morphology and color of hyphae. We have reconstructed the phylogenetic tree of a select group of Polyporus spp. using nucleotide sequences of the internal transcribed spacer region(ITS) region. The phylogenetic tree was constructed by using the neighbor-joining method. PELF primers of 20-mer were used to assess genetic diversity of preserved isolates. Sequence analysis showed that three strains were different species and four strains were identified completely different nomenclature. According to the analysis of ITS sequences, the genus Polyporus clustered into five distinct group, most of which correlated with species-groups identified by RAPD method. Four isolates included strain PM02 showed high similarity with P. arcularius, four isolates included strain PM03 high similarity with P. alveolaris, three isolates included strain PM01 high similarity with P. tuberaster, and PM 06 and PM04 high similarity with P. brumalis and P. squamossus. Isolates were collected in the United States(PM10, PM11) was identified as P. alveolarius and P. arcularius. RAPD analysis of genetic polymorphisms of genus Polyporus showed a very different band patterns. As the result of RAPD and ITS region sequences analysis for preserved isolates, it seems likely that 6 isolates of Polyporus spp. may be need to reclassify or eliminate from preserved catalogue.

면실박 대체 보리가루 첨가에 따른 느타리버섯의 생육 특성

이찬중,이은지,박혜성,공원식 한국버섯학회 2017 한국버섯학회지 Vol.15 No.3

This study was conducted to investigate the optimum additive ratio of barley flour when used instead of cotton seed meal for the cultivation of oyster mushrooms. Mycelial growth was the fastest to 11.4 cm in medium containing 5% barley flour and to 9.0 cm in medium containing 30% barley flour. The mycelial density was slightly lower at 5% and 10% of barley powder addition, but there was no significant difference between the other treatments. The yield of the fruiting bodies was 158 g/850 mL, which was highest on treatment with 10% barley flour as a substitute for cotton seed meal. However, this was lower on treatments with only barley flour than in the control (156 g/850 mL). The diameter of the pileus and the hardness of stipes were highest with 10% barley flour and 10% in cotton seed meal, respectively. The L value was highest with 10% added barley flour, but the a-value and the b-value were not significantly different between treatments. The pH of the mixed media before inoculation with Pleurotus ostreatus was 4.7–5.0, and the total nitrogen content was 1.7–2.2, which was slightly lower than that of the control. The total carbon content was 44.6–45.0, which was not significantly different from that of the control. However, the content of trace elements such as Cu and Fe was lower than those of the control.

버섯 수확후배지의 재활용 및 효과 분석

이찬중,전창성,정종천,공원식,서장선 한국버섯학회 2012 버섯 Vol.16 No.1

병재배 느타리버섯 ‘춘추2호’의 정밀재배관리를 위한 생육환경 분석

이찬중,이성현,이은지,박혜성,공원식 한국버섯학회 2018 버섯 Vol.22 No.1

본 연구는 병 재배 느타리버섯 정밀재배를 위한 최적 생육모델 개발을 위하여 느타리 ‘춘추2호’ 농가를 대상 으로 스마트팜 기술을 적용하여 생육환경을 분석한 결과를 보고하고자 한다. 실험 농가는 느타리 ‘춘추’를 병 재배하는 농가로 재배사 규모는 균상면적이 114m2, 균상형태는 2열 5단, 냉동기는 10마력, 단열은 샌드위치 판넬 100T, 가습기는 초음파 가습기 2대, 난방은 10KW를 사용하였고, 5,500병을 입병하여 재배하고 있었다. 생육조건은 배양병을 균긁기 후 버섯이 발이되는 4일차까지는 20°C의 온도를 유지하고 4일차에 뒤집기하면 서 5일에는 18°C, 6일차에는 16°C유지하고 수확기간인 7일차와 8일차에는 14°C를 유지하였다. 가습과 광 그 리고 환기는 on-off로 조절하였으며, 가습의 경우 뒤집기인 4일차까지 1분(on)-40분(off), 5일과 6일차는 가습 이 없고, 7일차에는 8분-25분, 8일차에는 1분-40분으로 가습하였다. 광은 조절기(1∼5)로 1일부터 4일차까지는 2정도의 광을 주고, 5일부터 8일까지는 1정도의 약한 광을 처리하였다. 환기는 1일부터 5일까지는 환기를 주 지 않았고, 6일과 7일차에는 2,200에서 1,500ppm으로 낮추어 조절하고, 8일차에는 1,000ppm을 유지하였다. 농 가에 재배하고 있는 ‘춘주2호’의 자실체 특성은 갓 직경은 26.5mm, 갓 두께는 4.9mm이며, 대 굵기는 8.9mm, 대 길이는 68.7mm였다. 대 경도는 3.9g/mm, 갓 경도는 0.9g/mm였고, 대와 갓의 L값은 78.2와 60.5이였다. 자 실체 수량은 166.8 g/850ml였고, 개체중은 127.7g/10unit였다. 느타리 ‘춘추2호’재배농가에서 생육환경 데이터 를 수집하기 위하여 설치한 환경센서부로 부터 버섯의 생육에 직접적으로 영향을 미치는 온도, 습도, 이산화 탄소 농도, 조도 등을 수집 분석하였다. 수집한 온도를 분석한 결과, 균 긁기한 후 입상후 1일차에는 19.5°C, 2일과 3일에는 20.1∼20.5°C, 4일차에는 21°C, 5일차에는 20.5°C, 6일차에는 18.1°C, 7일차에는 14.2°C, 8일차 에는 13.1°C를 유지하면서 버섯을 수확하였다. 습도를 분석한 결과, 균 긁기한 후 입상시 습도는 거의 100% 에 가까웠고, 버섯 발생 및 생육과정 중에도 습도는 거의 100%를 유지하였다. 실제도 농가에서는 가습을 on-off로 조절하고 있었지만 습도센서로 수집한 결과는 전과정이 거의 100%의 습도를 유지하였다. 이산화탄 소농도를 분석한 결과, 입상후 서서히 증가하였고, 4∼6일에는 5,500ppm까지 높게 유지하였으며, 7일과 8일 에는 환기를 통해 단계적으로 농도를 낮추어 수확기에는 1605ppm을 유지하였다. 조도를 분석한 결과, 느타리 버섯 입상후 수확기까지 거의 4∼ 8lux정도의 빛을 조사하면서 생육을 진행하였다.