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이진주,김동혁,김대근,Hannah Leah Simborio,민원기,이후장,장동일,장홍희,김석 경상대학교 농업생명과학연구원 2012 농업생명과학연구 Vol.46 No.5
Salmonellosis is a widespread bacterial zoonosis that commonly causes enterocolitis and foodborne poisoning leading to an extensive economic loss in domestic animal industry. Considerably, the emergence of multidrug resistant strains of Salmonella spp. induces further severe problems affecting public health. The present report was designated to investigate the antibacterial efficacies of three common disinfectants including an oxidizing compound disinfectant (OXC), a triple salt (TS) and a quaternary ammonium compound (QAC) against Salmonella typhimurium subjected to the preliminary changes of drug temperature. All solutions of three disinfectants were pre-incubated at different temperature (22, 37 and 63°C) for 1 h prior to exposure to bacteria. The disinfectants and bacteria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OMS) according to treatment condition. Under the DW condition, the disinfectant efficacy of the QAC at 63°C was higher than that of 22°C. Furthermore, under HW diluent the disinfectant efficacy of the TS pre-warmed at both of 37 and 63°C were increased compared to that of 22°C. Considerably, the efficacy of pre-warmed QAC at both of 37 and 63°C under the OMS diluent were higher than that of 22°C. Conclusively, prewarming at higher temperatures have positive effects on the stability of the antibacterial efficacies of TS and QAC.
Salmonella typhimurium에서의 치오레독신 유전자의 발현
이진주,임창진 강원대학교 기초과학연구소 1999 기초과학연구 Vol.10 No.-
치오레독신은 매우 작은 단백질임에도 불구하고 매우 다양한 기능을 갖고 있다. 여러 가지 효소들의 cofactor, disulfide bond의 환원, 여러 질환에의 관여 등 여러 기능이 알려져 있으며, filamentous bacteriophage의 assembly에도 필수적인 것으로 밝혀져 있다. 단백질 연구자들에게는 일종의 chemical probe로서 표면에 노출된 disulfide bridge를 확인하는 목적으로 치오레독신의 protein disulfide reductase 활성이 활용되고 있다. 여러 다른 종들의 치오레독신에 대해서는 연구가 활발히 진행되고 있지만 Escherichia coli와 유사한 enterobacteria인 Salmonella typhimurium에서는 치오레독신에 대한 연구가 덜 되어 있다. 본 연구에서는 Escherichia coli 치오레독신 reductase를 이용하여 Salmonella typhimunum의 치오레독신 활성을 알아보고자 하였다.
이진주,이길호,신지현 한국미생물학회 2014 The journal of microbiology Vol.52 No.10
The food-borne pathogenic bacteria Listeria monocytogenescan form biofilms on various surfaces including food-processingequipment. Biofilms offer survival benefits to the organismsentrapped against environmental insults. Moreover,the σB transcription factor of L. monocytogenes plays an importantrole in its survival under various stress conditions. In this study, we evaluated whether σB contributes to biofilmformation when L. monocytogenes is grown under varioustemperatures and media. When the wild-type strain wasgrown under static biofilm culture below ambient temperature(15°C) for 72 h, the difference in viable cell number (inboth planktonic and biofilm cells) between the wild-typeand ΔsigB mutant increased by adding NaCl to BHI broth(9% salt BHI > 6% salt BHI > BHI, w/v), and the specific activityof β-galactosidase was highly induced in the wild-typestrain grown in 6% salt containing BHI broth. Furthermore,we measured surface-adhered biofilm forming ability usingthe crystal violet staining method. The wild-type strain formeda four times larger biofilm than that of the ΔsigB mutant in6% salt-BHI medium at 15°C over a 72 h incubation and alsoshowed the highest level of β-galactosidase specific activity. However, both the wild-type and ΔsigB mutant L. monocytogeneswere defective for forming a biofilm in 9% salt-BHImedium at 15°C. Our results suggest that σB plays an enhancedrole in surface-adhered biofilm formation when L. monocytogenes encounters dual stress conditions, such as6% NaCl and low temperature.