고무줄자를 이용한 취혈방법에 대한 실험연구; 경혈학실습 시간의 경험

이준무 경락경혈학회 2008 Korean Journal of Acupuncture Vol.25 No.2

Objectives : Accurate location of acupoints is a prerequisite for both practice and research into acupoint specificity. Given that accurate location of acupoints is one of the most important issues in meridianology education, this study aimed at evaluating if the elastic ruler method for locating acupoints is suitable for meridianology practice class. Methods : Fifty-six students who took meridianology practice class participated in the experiment. Firstly, they were asked to locate PC6, CV12 and ST36 without any assisting tools and the distance between standard acupoint and their acupoint location was measured. Secondly, they were again asked to locate the same points this time with an elastic ruler. Then the first and second measurements were compared using a paired t-test.Results : Analysis of the results found using an elastic ruler significantly improved the difference between the student’s acupoint location and the standard acupoint: there was a significant difference between 1st and 2nd measurements for PC6 (1.87±0.29mm vs. 1.28±0.19mm, P=0.037) and for ST36 (2.70±0.22mm vs. 1.38±0.15mm, P<0.0001).Conclusions : Using an elastic ruler as an assisting tool for locating acupoints in meridianology practice class should be considered for improving accuracy in acupoint location.

분심기음약침(分心氣飮藥針)이 과산화지질(過酸化脂質)을 투여한 흰쥐의 혈장(血漿) 및 간장(肝臟)지질구성과 항산화(抗酸化)에 미치는 영향 -태충혈(太衝穴)과 내관혈(內關穴)을 중심으로-

이준무,김민기,Lee, Jun-Mu,Kim, Min-Ki 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.3

Effects of Boonsimgieum aqua-acupuncture at $Naegweun(HP_6)$ and $Taecheung(LV_3)$ on liver and plasma lipid composition and antioxidative capacity were investigated in rat fed high oxidized fat. Concentrations of plasma triglyceride, total cholesterol and LDL-cholesterol showed a tendency to increase in the high oxidized fat diet group. However these values showed a tendency to decease in aqua-acupuncture groups. HDL-cholesterol showed no significantly different in all the treatment groups. Liver total cholesterol values showed a high in control group, however other treatment groups showed no significantly different. Liver triglyceride concentration showed a high value in control group and $Naegweun(HP_6)$ aqua-acupuncture group showed lower value than $Taecheung(LV_3)$ aqua-acupuncture group. Plasma GOT and GPT values showed a tendency to increase in high oxidized fat diet group. However aqua-acupuncture groups showed a lower values than control group. The concentration of TBARS in liver and plasma showed a high values in high oxidized fat diet group, however these values showed a tendency to decrease in aqua-acupuncture group. Glutathione peroxidase, superoxide dismutase and catalase activity values showed a low values in high oxidized fat diet group, however these values showed a tendency to increase in aqua-acupuncture groups and in aqua-acupuncture groups, these values showed no significantly different.

東醫寶鑑의 觀点에서 본 肥滿의 鍼灸經穴學的 考察

이준무,심성윤 경락경혈학회 2003 Korean Journal of Acupuncture Vol.20 No.1

새로 개발한 한방 피부소양증 치료제에 관한 연구

이준무,Lee, Joon-Moo 대한한방안이비인후피부과학회 2008 한방안이비인후피부과학회지 Vol.21 No.3

Objectives : There have been few studies on external treatment for itching. A clinical study was performed to evaluate the effect of a newly developed herbal ointment. Methods : A newly developed herbal ointment made from Rumex japonicus Houtt, Prunus persica(Linne) Batsch, Sesamum indicum L., and Sus scrota domestics Brisson., was given to 49 participants with itching to administer for approximately a month. Demographic characteristics, treatment experiences, symptom change, and other comments were self-reported on a pre-defined questionnaire. Results : Most frequent complaint was itching (24.01%), followed by sensitivity (19.60%), dryness(18.62%), frustration (13.72%) and insomnia (12.25%). Of 41 participants who had been given treatment for itching, around a half of them (n=21) have been treated with Korean Medicine, but all of them tried Western Medicine as well. The effect of the ointment was evaluated as excellent or good/fair (67.32%). Continuity was reported as excellent or good/fair (51.0%) and fragrance (73.44%) and color (75.48%) was reported as excellent or good/fair. Convenience, however, was assessed as good/fair (67.32%) and as inconvenient (22.4%). Conclusion : The newly developed herbal ointment seems promising for relieving itching and further research to standardize and develop this kind of ointment is warranted.

驚風의 病因病理 및 症狀에 関한 文献的 考察

李俊茂,李珩九 대한한의학회 1983 대한한의학회지 Vol.4 No.1

시호(Bupleuri Radix)분말이 고콜레스테롤 급여 흰쥐의 체지질구성 및 TBARS량에 미치는 영향

李俊茂,李垠,崔武永 대한본초학회 2000 大韓本草學會誌 Vol.15 No.1

Effects of powdered siho(Bupleuri Radix) on lipid composition and TBARS were investigated in rat fed high cholesterol. Plasma total cholesterol showed a tendency to increase in cholesterol group(P<.OS). However, in the cholesterol group, contents of serum total cholesterol showed no significantly different among treatments(P>.OS). Plasma HDL-cholesterol and triglyceride contents showed no significantly different among treatments(P>.OS). Liver total cholesterol showed a tendency to increase in cholesterol group, however these values showed no significantly different among all the treatments(P>.05). Liver triglyceride contents showed no significantly different among all the treatments(P>.05). Contents of plasma and liver TBARS were high (Pc.05) in control group fed high cholesterol only. however these values were lower by powdered siho(Bupleuri Radix) feeding. Glutathione peroxidasc activity showed low in control group fed high cholesterol only(P<.OS), however these values showed a tendency to increase(P<.OS) by powdered ailio(Bup1euri Radix) feeding

최근 중국의 중ㆍ서의 결합에 관한 연구

이준무,이선동 대한한의학회 1996 대한한의학회지 Vol.17 No.2

제조 藥針의 抗癌作用에 關한 硏究

李俊茂,河智容 대한동의병리학회 2000 동의생리병리학회지 Vol.14 No.2

최근 과학기술의 놀라운 발전에 힙입어 질병이 하나씩 정복되어 가고 있으나 평균수명의 연장 및 생활수준의 향상으로 인한 고지방식 과다섭취, 스트레스 등으로 인하여 질병으로 인하여 종양으로 인한 사망률이 증가하고 있는 실정이므로 종양의 치료방법이 다양한 방법으로 시도되고 있으며 약침을 이용한 방법도 이중 하나이다. 제조는 예로부터 破血, 行瘀, 散結, 通乳하는 효능이 있는 것으로 알려져 있어 이를 산지별로 다양한 방법으로 유효성분을 추출한 후 중완과 족삼리에 약침처리를 하여 담암생쥐의 생존능, 종양의 억제능, 시험관내세포독성능 실험을 수행하여 항암능을 살펴보고자 하였다. Median survival time에서 Normal군에서는 22.5일을, Control군에서는 24일을 보여 약침 자극만으로도 6.67%의 생존율이 연장되었으며 Hd군과 Jd군은 26.5일을 보여 Control군에 비해 10.42%의 증가율을 보였으며 Hw군은 28일에 16.67%의 증가율을, Jw군은 29일에 20.83%의 증가율을 보여주어 약침제제 사용의 경우뿐만 아니라 단순한 경혈 자극만으로도 생존율이 증가하였음을 알수 있었다. 담암생쥐의 종양을 적출하여 무게를 잰 결과 Control군에 비해 Hwt군만을 제외하고는 모두 유의성 있는 억제율을 보여주고 있었으며 Hw군과 Jw군이 각각 1.851±0.027g, 1.827±0.015g으로 가장 높은 효과를 보여주었다. Sarcoma-180 세포를 이용한 시험관내 세포독성능 실험에서 생존율에 의한 IC_50은 약침제조액을 1로 보았을 경우 Ha군은 0.6을 Ja군은 0.375를 보였다. 이를 종합하면 중완과 족삼리에 단순히 자극을 준 것만으로도 생존율이 어느 정도 연장되어 경혈의 효과를 입증한 것으로 보여지며 여러 추출법에 있어서 물추출알콜 침전법이 다른 추출법에 비해 상대적으로 유효한 성분을 많이 얻었던 것으로 보아 추후 유효성분의 규명과 규격화 및 안전도에 관한 후속 연구가 필요할 것으로 사료된다. In order to investigate the antitumor effect by Aqua-acupuncture liquor made by precipitation method in alcohol from Holotrichia after B-16 cells were transplanted in C57BL/6 mice. Experimental studies were performed for measurance of metastasis, cell cytotoxicity in vitro, life extention, weight of cancer. The first station investgated the effect of Aqua-acupuncture liquor made by precipitation method in alcohol from Holotrichia boiled with water and Aqua-acupuncture Holotrichia liquid by distilled method on the survival time of the mice transplanted by sarcoma-180 tumor cell, inhibition of Holotrichia and Saline on B016 Tumor in Mice. The measurement of In vitro cytotoxicity showed a tendency to inhibite cell, the size of edema showed significant value and the survival time. The results were summarized as follows: 1. Mean survival time in Hd(10.42%), Jd(10.42%), Hw(16.67%), Jw(20.83%)group was prolonged, as compared with control group significantly(P< 0.05). 2. On the weight of solid tumor, Holotrichia-treated group was less than control group with significance(P< 0.05). 3. On the MTT assay, cell viability was significantly inhibited by 1/well, 0.5/well, 0.25/well, 0.125/well. 0.625/well of Aqua-acupuncture liquor made by precipitation method in alcohol from Holotrichia(produced in Korea) boiled with water, 1/will, 0.5/well, 0.25/well, 0.125/well of Aqua-acupuncture liquor made by precipitation method in alcohol from Holotrichia(produced in China) boiled with water and in case of korea and china of Aqua-acupuncture Holotrichia liquid by distilled method concentration inhibited cell viability significantly(P< 0.05). IC_50 for cell viability was 2.17 ㎍/well.

적소두증유약침(赤小豆蒸溜藥鍼)이 비만(肥滿)쥐의 지질구성(脂質構成), 간장기능(肝臟機能), 항산화효과(抗酸化效果) 및 분자생물학적(分子生物學的) 특성(特性)에 미치는 영향(影響)

이준무,지준환,Lee, Jun-Mu,Ji, Jun-Hwan 대한약침학회 2005 Journal of pharmacopuncture Vol.8 No.2

Effects of Phaseoli Semen rubra Herbal-acupuncture at zusanli(ST-36), Quchi(LI-11) and Sanyinjiao(Sp-6) on lipid composition, liver function, oxidative capacity and molecular biological aspects were investigate in high fat diet induced obese rats. Forty male Sprague-Dawley rats weighing about 400g were divided into 4 groups according to body weight and raised four weeks with control, zusanli(ST-36), Quchi(LI-11) and Sanyinjiao(Sp-6) Herbal-acupuncture groups. 1. Plasma total cholesterol and LDL-cholesterol showed a low values in the (ST-36) and (LI-11) Herbal-acupuncture groups and HDL-cholesterol showed a high values in the (ST-36) Herbal-acupuncture groups. 2. Plasma triglyceride and glucose showed a low values in the (ST-36) and (LI-11) Herbal-acupuncture groups. 3. The contents of plasma free fatty acids showed a tendence to decrease in the Herbal-acupuncture groups, however in the Herbal-acupuncture groups, the values showed no significantly different. 4. The activities of AST and ALT showed no significantly different in all treatment groups. 5. The contents of plasma ${\beta}-lipoprotein$ and free fatty acids showed a tendency to decrease in the Herbal-acupuncture groups compared to those of control group. In the Herbal-acupuncture groups, the values of (ST-36) and (LI-11) Herbal-acupuncture groups showed a low in the acupuncture groups. 6. Liver total cholesterol and triglyceride showed a low values in the (ST-36) and (LI-11) Herbal-acupuncture groups. 7. Contents of plasma TBARS showed a low values in the (ST-36) and (LI-11) Herbal-acupuncture groups, however contents of liver TBARS showed no significantly different among treatment groups. 8. Values of liver glutathione peroxidase and catalase activity showed a tendency to increase in the (ST-36) and (LI-11) Herbal­acupuncture groups. Values of liver super oxide dismutase activity showed a high in the (ST-36) Herbal-acupuncture groups compared to those of other groups. 9. Expression of apo-B mRNA in liver cell showed a low in (ST-36) Herbal-acupuncture groups, however expression of apo-E mRNA showed a high in Herbal-acupuncture groups and $TNF-{\alpha}$ mRNA in adipose cell showed no different among all the treatment groups. Expression of Leptin mRNA showed a tendance to decrease in (ST-36) Herbal-acupuncture group. 10. Histological character of liver, those of (ST-36) Herbal-acupuncture group showed a good, however other treatment groups showed slight vasodilation and slight fat accumulation. These results indicated that Phaseoli Semen rubra Herbal-acupuncture at (ST-36) and (LI-22) suppressed adipose tissue mass and lipid peroxidation and increased antioxidant system.

肝兪 및 日月 靑蒿藥鍼이 高脂肪 給與 흰쥐의 血淸 脂質構成에 미치는 影響

李俊茂 경락경혈학회 2003 Korean Journal of Acupuncture Vol.20 No.3