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북방대합 ( Spisula sachalinensis ) 의 생식주기
이정용(Jeong Yong Lee),장영진(Young Jin Chang),이채성(Chae Sung Lee) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.1
In order to obtain the basic information for seedling production of surf clam, Spisula sachalinensis, annual reproductive cycle was investigated from March 1994 to May 1995. S. sachalinensis was dioecious. The gonads are located between the digestive diverticula and muscle tissue of the foot. The condition factor was reached its maximum (0.19) in June, and then decreased to 0.16 in July. In November, the value was the lowest and then increased slowly. The age and minimum size for sexual maturation were 2 years old and about 50 ㎜ in shell length, respectively. However, less than 10% of sexually undifferentiated individuals were larger than 80 ㎜ in shell length. On the other hand, the size of mature oocytes was ranged to 50∼60 ㎛ in diameter and testis-ova was observed in testis of the mature stage. Annual reproductive cycle could be classified into five successive stages : multiplicative (October to January), growing (February to April), mature (April to June), spawning (June and July) and degenerative and resting (August and September) stages.
기수재첩(Corbicula japonica)의 정자형성과정과 정자 미세구조
이정용 ( Jeong Yong Lee ),김완기 ( Wan Ki Kim ),이채성 ( Chae Sung Lee ),장영진 ( Young Jin Chang ) 한국수산과학회 2004 한국수산과학회지 Vol.37 No.4
N/A Spermatogenesis and sperm ultrastructure of the marsh clam (Corbicula japonica) were investigated by electron microscopic observations. Testis of the marsh clam consists of n㎛erous spermatogenic follicle containing germ cells in the different developmental stages. Spermatogonia are located nearest the outer wall of the follicle, while spermtocytes and spermatids are positioned nearer to the l㎛en. Spematogonia are oval-shaped and about 5 ㎛ in diameter. Spermatogonia develop into spermatocyte, spermatid and spermatozoon. In the spermatid to about 2 ㎛ in diameter, cytoplasm decreases and mitochondria move to the base of the nucleus and fuse into several spheres, the centrioles become orthogonally oriented, a flagell㎛ appears, and an acrosomal vesicle forms. The mature sperm has primitive type, consisting of a head, a midpiece and a tail. The sperm was arrow-shaped, and its head is about 8 ㎛ long and comprised of a long nucleus and an acrosome. The four mitochondria encircled the centrosome in midpiece. The flagell㎛ had the classical 9+2 axoneme structure, and axonemal lateral fins in the tail were observed.
북방대합 , Spisula sachalinensis 수정을 위한 최적방법
이정용(Jeong Yong Lee),장윤정(Yun Jeong Chang),전민지(Min Jee Chun),장해진(Hae Jin Chang),장영진(Young Jin Chang) 한국수산과학회 2002 한국수산과학회지 Vol.35 No.2
이 연구에서 북방대합의 정자 운동성 및 수정률과 부화율 향상을 위한 수정방법을 조사하였다. 북방대합 원정액의 정자 농도는 2.02×10 exp (10)/mL였으며, 채정 직후에는 원정액이나 해수중의 정액에서 모두 96.0%의 정자가 전진운동을 하였다. 절개법으로 얻어진 알과 정자는 각각 채란 4시간 이내에, 채정 1시간 이내에 수정하는 것이 효과적이었다. 인공수정을 위한 알 1개에 대한 정자수는 10∼100개에서 수정률과 부화율이 모두 높았으며, 수정 후 1시간 이내에 세란하는 것이 효과적이었다. 최적의 수정 및 발생을 위한 알의 수용밀도는 100∼200 개/mL였다. In order to obtain the basic information for the seedling production of surf clam, Spisula sachalinensis, sperm motility and optimal method for fertilization were investigated. Sperm concentration of S. sachalinensis milt was 2.02 × 10 exp (10) cell/mL and approximately 96.0% of sperm showed forward movement after exposure to seawater. When sperm and eggs obtained by incision method were fertilized in 1 hour and 4 hours, respectively, high fertilization and hatching rate were achieved. The optimal sperm concentrations and egg density for fertilization and hatching were 10∼100 inds./egg and 100∼200 inds./mL sea water, respectively.
민들조개 ( Gomphina melanaegis ) 의 생식소 발달과 생식주기
이정용(Jeong Yong Lee),박영제(Young Je Park),장영진(Young Jin Chang) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.2
Gonadal development and reproductive cycle of Gomphina melanaegis collected in the coastal waters of Chumunjin, Korea were investigated monthly from April 1996 to April 1997. G. melanaegis was dioecious. The gonads were located between the digestive diverticula and muscle tissues of the foot. The ovary was composed of a number of ovarian sacs, and the testis was composed of several testicular tubules. The flesh weight rate was reached the maximum in August (23.0%), and then decreased to 19.8% in September. In March, the value was reached the minimum (17.8%) and then increased. The size of mature oocyte was ranged 50∼60㎛ in diameter and had a germinal vesicle with a nucleolus. Mature oocyte contained a large number of yolk granules and lipid granules in its cytoplasm. The spermatozoon was consisted of a conical nucleus with acrosome, a middle piece contaiaing four mitochondria and proximal and distal centrioles, and a flagellum. Sex ratio (♂/♀) and minimum size for sexual maturation of G. melanaegis were 0.79 and about 25 ㎜ in shell length, respectively. The reproductive cycle could be classified into five succesive stages: multiplicative (December to March), growing (April and May), mature (June), spawning (July and August), and degenerative and resting (September to November) stages.
저혈당성 경련으로 내원한 환자에서 진단된 Silver-Russell 증후군 1례
이정용(Jung Yong Lee)ㆍ김영아(Young A Kim)ㆍ이경연(Kyung Yeon Lee)ㆍ오기원(Ki Won Oh)ㆍ김자형(Ja Hyeong Kim)ㆍ김준성(Joon Sung Kim)ㆍ정진영(Jin Young Jeong)ㆍ박상규(Sang Kyu Park) 대한소아신경학회 2010 대한소아신경학회지 Vol.18 No.1
저자들은 저혈당성 경련을 주소로 내원한 4세 여아에서 자궁 내 성장 지연, 출생 후 지속되는 저신장과 저체중, 몸에 비해 상대적으로 큰 머리, 신체의좌우 비대칭, 특징적인 얼굴 모양을 보인 Silver-Russell 증후군 1례를 경험하였기에 문헌 고찰과함께 보고하는 바이다. The Silver-Russell syndrome(SRS) is a clinically heterogeneous syndrome characterized by intrauterine and postnatal growth retardation with spared cranial growth, characteristic facial features, and body asymmetry. Although mild to moderate hypoglycemic symptoms occasionally appear in children with SRS especially those who are not fed frequently and regularly, hypoglycemic seizures rarely occur. We report a rare case of SRS which was diagnosed in a 4-year-old female who admitted with hypoglycemic seizure. The patient showed the haracteristic features of SRS. Endocrinologic studies were normal except for partial growth hormone insufficiency. To prevent seizures and chronic neurologic deficits in children with SRS, the early recognition and appropriate management of hypoglycemia is critical.
북방대합 (Spisula Sachalinensis) 생식세포의 미세구조
이정용 ( Jeong Yong Lee ),장윤정 ( Yun Jeong Chang ),장영진 ( Young Jin Chang ) 한국수산과학회 2003 한국수산과학회지 Vol.36 No.2
N/A Ultrastructure of germ cell during gametogenesis of surf clam, Spisula sachalinensis were investigated by electron microscopic observations. Oogonia are oval in shape and 5~6 ㎛ in diameter. They contain a large nucleus and have several mitochondria in the cytoplasm. Vitellogenic oocytes grew attached by a stalk to the germinal epithelium in the ovarian sac and their cytoplasm contained many yolk granules, lipid granules and mitochondria. Mature oocytes measuring 50~60 ㎛ in diameter have a nucleus containing an electron dense nucleolus, and a lots of yolk granules, lipid granules, mitochondria, rough endoplasmic reticulum and cortical granules were present in the cytoplasm. The surface of the plasma membrane in mature oocytes was formed of microvilli approximately 1.5 ㎛ long and enveloped in the vitelline layer. Spematogonia are oval in shape and about 5 ㎛ in diameter. Spramatogonia develop into spermatocyte, spermatid and spermatozoon. The spermatozoon consisted of the head, midpiece and tail. The sperm head with diameter of about 1.5 ㎛ was ovoid, and contained the nucleus which consisted of acrosome. The four mitochondria encircled the centrosome in midpiece. The flagellum measuring about 40 ㎛ long had the classical 9+2 axoneme structure.