Complete genome sequence of Variovorax sp. PMC12, a plant growth-promoting bacterium conferring multiple stress resistance in plants

이신애,김현수,김이슬,상미경,송재경,원항연,Lee, Shin Ae,Kim, Hyeon Su,Kim, Yiseul,Sang, Mee Kyung,Song, Jaekyeong,Weon, Hang-Yeon The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4

본 연구에서 생물 및 비생물학적 스트레스에 내성을 유도하는 식물 생육 촉진 세균인 Variovarx sp. PMC12 균주의 유전체 염기서열을 분석하였다. PMC12 균주의 유전체는 5,873,297 bp와 1,141,940 bp 크기의 원형 염색체 2개로 구성되었다. 총 6,436개 단백질 유전자, rRNA 9개, tRNA 64개, ncRNA 3개와 유사유전자 80개가 확인되었다. 유전체상에서 발견된 1-aminocyclopropane-1-carboxylate (ACC) deaminase, 항산화 활성, 인산 가용화, 프롤린 생합성, 시드로포어 생합성과 관련된 유전자들은 PMC12 균주가 염, 온도, 병원균에 대한 스트레스에 대한 식물의 내성 유도와 관련되어 있을 것으로 판단된다. Variovorax sp. PMC12 is a rhizobacterium isolated from tomato rhizosphere and enhanced the plant resistance to abiotic and biotic stresses. Here we present the complete genome sequence of strain PMC12. The genome is comprised of two circular chromosomes harboring 5,873,297 bp and 1,141,940 bp, respectively. A total of 6,436 protein-coding genes, 9 rRNAs, 64 tRNAs, 3 ncRNAs, and 80 pseudogenes were identified. We found genes involved in 1-aminocyclopropane-1-carboxylate (ACC) deaminase, antioxidant activity, phosphate solubilization, and biosynthesis of proline and siderophore. Those genes may be related to capability of improving plant resistance to various stresses including salinity, cold temperature, and phytopathogen.

Complete genome sequence of Chryseobacterium sp. T16E-39, a plant growth-promoting and biocontrol bacterium, isolated from tomato (Solanum lycopersicum L.) root

이신애,김상윤,상미경,송재경,원항연,Lee, Shin Ae,Kim, Sang Yoon,Sang, Mee Kyung,Song, Jaekyeong,Weon, Hang-Yeon The Microbiological Society of Korea 2017 미생물학회지 Vol.53 No.4

토마토 뿌리에서 분리한 Chryseobacterium sp. T16E-39 균주는 식물생육촉진과 역병, 시들음병에 대한 억제효과가 있었다. 이 균주의 유전체 염기서열은 4,873,888 염기쌍이었으며 G + C 함량은 35.22%이었다. 이 유전체는 4,289개 단백질 유전자, 15개 rRNA 유전자, 71개 tRNA 유전자를 포함하였다. T16E-39 균주의 유전체에서 인산가용화, 식물호르몬 조절, 항산화 활성, 키틴 분해, 제9형 분비시스템에 관여하는 유전자를 확인하였으며, 이들 유전자는 식물의 생육을 촉진하고 병발생을 억제하는 기작과 관련되어 있을 것으로 판단된다. Chryseobacterium sp. strain T16E-39, isolated from roots of a tomato plant, promotes plant growth and suppresses phytophthora blight and bacterial wilt diseases. The complete genome of strain T16E-39 consists of a circular chromosome with 4,872,888 base pairs with a G + C content of 35.22%. The genome includes 4,289 coding sequences, 15 rRNAs, and 71 tRNAs. We detected genes involved in phosphate solubilization, phytohormone regulation, antioxidant activity, chitin degradation, and the type IX secretion system (T9SS) that may be related to growth promotion and disease suppression in plants.

토마토 뿌리에서 분리한 식물생육촉진 세균 Bacillus velezensis T20E-257균주의 유전체 염기서열

이신애,김상윤,상미경,송재경,원항연,Lee, Shin Ae,Kim, Sang Yoon,Sang, Mee Kyung,Song, Jaekyeong,Weon, Hang-Yeon 한국미생물학회 2017 미생물학회지 Vol.53 No.4

Bacillus velezensis T20E-257 was isolated from the root tissue of a tomato plant and exhibited plant growth-promoting activity. Here we present the complete genome of strain T20E-257. The genome contains 3,900,066 base pairs with a G + C content of 46.7% in 2 contigs. The genome includes 3,708 coding sequences, 27 rRNAs, and 86 tRNAs. We found gene clusters encoding secondary metabolites with an antimicrobial activity and genes related to the production of indole-3-acetic acid and 2,3-butanediol, which play a role in plant growth and health. 토마토 뿌리에서 분리한 Bacillus velezensis T20E-257 균주는 식물촉진효과가 있었고, 본 연구에서 T20E-257 균주의 유전체 서열을 해독하였다. 유전체 초안에서 포함된 2개 contig는 총 염기서열이 3,900,066 bp고, G + C content가 46.7%이었다. 유전체에서 단백질 유전자 3,708개, rRNA 유전자 27개, tRNA 유전자 86개를 확인하였다. 항균활성을 가지는 2차 대사산물 생합성 관련 유전자군과 식물생육촉진에 관여하는 IAA와 2,3-butadiol 생합성 관련 유전자를 T20E- 257 균주 유전체에서 확인하였다.

림프관종과 감별이 어려웠던 비장 가성낭종 1예

이신애 ( Shin Ae Lee ),한상영 ( Sang Young Han ),이은주 ( Eun Joo Lee ),권병표 ( Byung Pyo Kwon ),고인영 ( In Young Koh ),최해종 ( Hae Jong Choi ),김민찬 ( Min Chan Kim ),조진한 ( Jin Han Cho ) 대한소화기학회 2006 대한소화기학회지 Vol.48 No.6

Splenic pseudocyst is a rare complication of abdominal trauma. Although it is rare, splenic pseudocyst is well-documented in the literature. According to the current classification, approximately 30% of all splenic cysts or pseudocysts result from direct abdominal trauma. In addition, chronic pancreatitis leads to change of nearby organs with possible acute and chronic complications including splenic lesions. This unusual complication can occur in both emergent and nonemergent conditions. The useful diagnostic procedures to assess intrasplenic pseudocyst are sonogram, CT scan, splenic scan, and occasionally angiography. However, definite diagnosis of pseudocyst is possible only after splenectomy when the absence of epithelial lining is confirmed histologically. Splenic pseudocyst requires surgical resection. We experienced a 31-year-old man who confirmed of warmness in the left side of back with left upper quadrant abdominal pain for several months. First impression was splenic lymphangioma based on CT scan and sonogram finding. Splenectomy was performed. Microscopic examination re­vealed splenic pseudocyst with fibrous capsule without epithelial lining. (Korean J Gastroenterol 2006;48:427-430)

트로피컬 하우스에서 나타나는 켈틱 음악의 특징 연구

이신애(Shin-Ae Lee) 한국산학기술학회 2020 한국산학기술학회논문지 Vol.21 No.9

본 논문은 기존의 트로피컬 하우스의 정의와 다르게 접근한다. 일렉트로닉 음악 장르인 딥 하우스, 칠 아웃, 댄스홀로부터 파생되었다는 것이 기존의 정의이다. 이와 달리 본 연구에서는 트로피컬 하우스가 일렉트로닉 음악뿐만 아니라 다른 예술 양식의 요소를 포함하고 있음을 이야기한다. 바로 아일랜드의 전통 음악인 켈틱 음악의 요소들이다. 어떤 이유에서 트로피컬 하우스 장르와 켈틱 음악 장르의 융합이 용이했는지에 관하여 설명한다. 그리고 트로피컬 하우스가 켈틱음악의 요소를 가지고 있다는 것을 8곡의 파퓰러 음악을 통하여 분석한다. 트로피컬 하우스의 선율, 화성, 리듬, 보컬창법, 악기, 곡의 구조가 켈틱 음악의 요소와 교집합이 있다는 것을 확인할 수 있다. 본 논문의 요지는 켈틱 음악의 요소가 트로피컬 하우스라는 장르에서 발견되었음을 주장하는 것이다. 더불어 창작자들은 세계 전통음악을 비롯한 다양한 음악들에 관심을 갖을 필요가 있다. 독창적인 음악을 만들기 위해 오늘날의 음악과 다양한 장르의 음악을 어떻게 접목시킬 수 있을지에 대해 고민해볼 필요가 있음을 시사한다. This research approach is different from the existing definition of Tropical House. It was derived from an electronic music genre (deep house, chill out, dance hall). However, this study explains that Tropical House contains elements of other art forms as well as electronic music. These are the elements of Ireland"s traditional music (Celtic music). This study explains why the fusion of Tropical House and Celtic music was easy. And it shows that elements of Celtic music are included in Tropical House through eight popular songs. It can be confirmed that the melody, harmony, rhythm, singing method, instrumentation, and structure of Tropical House are intertwined with the elements of Celtic music. The key point of this research is to suggest that Celtic musical elements are found in the Tropical House genre. In addition, creators need to pay attention to various types of music, including traditional music from around the world. It is suggested that creators need to think about how to combine today"s music with various other genres to create original music.

흡연청소년의 성별에 따른 금연시도 관련 요인 : 제16차 청소년건강행태조사를 중심으로

이신애(Shin-Ae Lee),송혜영(Hye-Young Song) 한국보건복지학회 2022 보건과 복지 Vol.24 No.2

본 연구는 흡연 청소년의 성별에 따른 금연시도에 영향을 미치는 요인을 파악하기 위하여 청소년건강행태조사(2020) 자료를 활용하여 2차 분석을 수행한 단면 조사연구이다. 중•고등학교에 재학 중인 흡연 청소년 2,437명을 대상으로 교차분석과 로지스틱 회귀분석을 시행하였다. 대부분의 학생들은 중학생 때 처음 흡연을 시작하였고 흡연 청소년의 금연시도율은 69.4%이었다. 로지스틱 회귀분석 결과, 남학생은 격렬한 신체활동(OR=1.567, p<.001), 우울(OR=1.343, p=.022), 자살 생각(OR=0.638, p=.006), 처음 흡연 시작시기인 중학교(OR=2.372, p=.011), 흡연량(OR=0.584, p<.001)이 유의한 변수로 나타났으며 여학생은 스트레스(OR=1.744, p=.037)와 처음 흡연 시작시기인 초등학교(OR=5.317, p=.005), 중학교(OR=6.213, p=.001), 고등학교(OR=4.439, p=.008)가 유의한 관련이 있었다. 이처럼 흡연 청소년의 성별에 따라 금연시도에 차이가 있으므로 남자 청소년은 신체활동을 증진하는 금연 프로그램, 여자 청소년은 스트레스와 같은 내적 감정에 중점을 두는 상담 프로그램을 제안하며 향후 남녀의 차별화된 금연상담 정책과 금연 프로그램 접근이 이루어져야 할 것이다. This study was conducted to identify factors affecting smoking cessation attempts according to gender in adolescents who smoke. This was a cross sectional study using the results of the Youth Health Behavior Survey (2020). The participants were 2,437 smoking adolescents enrolled in middle and high school. The data was analyzed by Rao-Scott χ₂-test and logistic regression analysis with SPSS/WIN 25.0. Most of the students started smoking when they were in middle school, and the smoking cessation rate was 69.4%. There were significant differences in intense physical activity (OR=1.567, p <.001), depression (OR=1.343, p =.022), suicidal ideation (OR=0.638, p =.006), and first smoking period (OR=2.372, p =.011) and smoking amount (OR=0.584, p <.001) in male adolescents. For female adolescents, stress (OR=1.744, p =.037) and first smoking period were elementary school (OR=5.317, p =.005), middle school (OR=6.213, p =.001), and high school (OR=4.439, p =.008) was significant variable. According to the results of the differences in smoking cessation attempts according to the gender of smoking adolescents, we propose a smoking cessation program that promotes physical activity for male adolescents and a counseling program that focuses on internal emotion such as stress for female adolescents.

크론병 환자의 삶의 질 모형

이신애(Lee, Shin Ae) 기본간호학회 2020 기본간호학회지 Vol.27 No.4

Purpose: The purpose of this study was to develop and test a structural model for quality of life (QoL) in patients with Crohn’s disease. Methods: A total of 273 patients with Crohn’s disease were surveyed during their outpatient visits in the department of gastrointestinal internal medicine. Assessment tools consisted of the Inflammatory Bowel Disease Questionnaire for QoL, Perceived Stress Scale for stress, Beck Depression Inventory-II for depression, Fatigue Scale for fatigue, and Multidimensional Scale of Perceived Social Support for social support. Structural equation modeling was used for data analysis, descriptive statistics, Pearson correlation analysis, and factor analysis. Results: The modified model showed a good fit for the data. The model fit indices were x²=9.36 (p=.671), RMSEA=.00, SRMR=.03, GFI=.99, AGFI=.97, NFI=.96, and CFI=1.00. The structure of the model demonstrated that disease severity, depression, stress, and social support explained 67.9% of the QoL of Crohn’s disease patients. The results showed that social support for patients with Crohn’s disease was high in terms of QoL; furthermore, when disease severity, depression, and stress were low, QoL was high. Conclusion: The results of this study show that disease severity, depression, stress, and social support have an important effect on the QoL of patients with Crohn’s disease. Therefore, it is necessary to take these variables into consideration when developing nursing interventions.

시상하부 과오종과 연관된 성조숙증을 동반한 신경섬유종증 1예

이신애(Shin Ae Lee),김진호(Jin Ho Kim),김선준(Sun Jun Kim) 대한소아신경학회 2018 대한소아신경학회지 Vol.26 No.1

신경섬유종증 1형은 흔히 보일 수 있는 신경피부증후군의 하나로, 담갈색 반점, 겨드랑이 주근깨 모양 색소반, 피부신경섬유종 그리고 Lisch 소결절 등을 특징적으로 나타내며, Neurofibromin을 발현하는 NF1 유전자의 변이가 이 질환의 원인으로 알려져있다. NF1 환아에게 있어서 성조숙증을 같이 동반하는 경우가 있으며, 이런 경우 대부분 시신경 종양과 연관되어있다. 저자들은 NF1에서 시상하부 과오종과 연관된 성조숙증을 동반한 케이스를 보고하고자 하며, 이 환아의 경우 지금까지 보고되지 않았던 NF1 exon 5의 14-bp 결실 돌연변이를 동반하고 있는 바이다. Neurofibromatosis type 1 (NF1) is a common neurocutaneous syndrome that presents with multiple café-au-lait spots, skinfold freckling, dermatofibromas, neurofibromas, and Lisch nodules. Mutations of the NF1 gene, encoding the protein neurofibromin, have been identified as the cause of this disease. NF1 can also present with precocious puberty and be associated with optic pathway tumors. Hypothalamic hamartoma as the cause of precocious puberty in patients with NF1 has been rarely described in the literature. Here, we report the findings for a patient with NF1 and precocious puberty associated with a hypothalamic hamartoma who had a newly discovered 14-bp deletion mutation in exon 5 of NF1. To our knowledge, this is the first time this combination is reported in the literature.

수업 준비를 위한 초등 과학 교사용 지도서 활용 실태 -2009 개정 과학과 3-4학년을 중심으로-

이신애 ( Shin Ae Lee ),임희준 ( Hee Jun Lim ) 한국초등과학교육학회 2016 초등과학교육 Vol.35 No.2

This study aims to investigate how elementary teachers use teachers`` guide in preparation for science lessons. First, different uses of teacher``s guide were analyzed. Second, how and why teachers use each section in teacher``s guide were analyzed. For the study, 24 elementary school teachers were interviewed in depth. The semi-structured in-depth interviews were conducted individually and/or in small group, and additional interviews were held when necessary. The results showed that most of the teachers used teacher’s guide only substitutionally, and some teachers rarely use teacher``s guide, while only 3 out of 24 teachers used teacher’s guide in detail. The reasons that teachers used the teacher’s guide substitutionally or rarely were that most science lessons include experiments, and science textbook itself provides enough information for preparation of science lessons for 3rd and 4th grade students. The results also revealed that only few teachers read the general guideline in teacher``s guide. Some sections of teacher``s guide were not used. The sections that many teachers used were the aims of lesson, the learning system of the unit, background knowledge, flow of lesson, learning contents and activities. This study specifically examined the actual use of teacher’s guide for lesson preparation and discussed implications for the development of more helpful teacher``s guide.

만삭임신후에 동반된 융모상피암

이춘배(Chun Bae Lee),장윤주(Youn Choo Jang),이진호(Jin Ho Lee),이정석(Jung Seok Lee),이지연(Ji Yeon Lee),이신애(Shin Ae Lee),김종우(Jong Woo Kim) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.7

Gestational trophoblastic tumor became one of the curable disease due to the development of chemotherapy, appropriate follow up of β- human chorionic gonadotropin and others. But metastatic trophoblastic tumor still shows high mortality rate because of resistance to the chemotherapy and large tumor burden.One of the important prognostic factors determining the prognosis of gestational trophoblastic tumor is antecedent pregnancy, which affects the response to therapy. Choriocarcinoma following term pregnancy is rare one with the incidence of one per 160,000 pregnancies in United States and the prognosis is poor because of late diagnosis and combined high risk prognostic factors.We present a choriocarcinoma following term pregnancy that required emergency total abdominal hysterectomy due to profuse bleeding with brief review of literatures.