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Amelogenin 유전좌를 이용한 성별검색의 유용성에 관한 연구
이숭덕,이정빈,이윤성 大韓法醫學會 2000 대한법의학회지 Vol.24 No.1
The aim of this study was to validate the sex typing based on amplification of X-Y homologous Amelogenin locus in Korean including mutation rate in this locus. It was found that there was no case with reported mutation that may hinder the exact sex typing among 240 Koreans, and the sex typing was successful even with subnanogram quantities of male and female DNA. There was no difference in the sensitivity of reaction among male and female. Differential amplification between X and Y amelogenin bands in some samples was noted, and dilution study revealed that this phenomenon was more frequent when the quantity of sample was low, usually less than 10 ng. That phenomenon was variable between amplification reactions, and was also dependent on different Taq enzyme used for the amplification. When there was differential amplification, the intensity ratio (Y band/X band) ranged about 0.68-0.87.
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이숭덕 사법발전재단 2014 사법 Vol.1 No.28
The most important factors in appraisal are the appraiser who conducts accurate job and the judge who needs the expert witness to make a decision. It is not difficult to hear complaints about appraisal in the medical field. We have to concentrate on those two factors, appraiser and judge, to solve those problems. First, it is essential to upgrade the level of an appraiser. For this, it is essential to understand difference between legal process and medical practice, and then, to select proper experts who can execute an appraisal. Establishment of administrative procedures is also important for fair proceeding. In this regard, an organization or the court specialized in appraisal may be necessary. The valuation fee is not only return for appraisal but also the means of asking responsibility and condor. To disclose the result of an appraisal or to give feedback on the level of contribution to trial may contribute to increase the quality of appraisal. It is recommendable that the judge participates in appraisal positively and gives attention to its process carefully. The appraisal is a procedure set forth in the law. Thus, without the involvement of judges as the consumer of appraisal, efforts for improvement of the level of appraisal seem to be limited. There are quite a number of procedural matters that only a court or a judge can solve. It is preferable to establish the forum for judges and experts to communicate on the subjects without interruption because appraisal is the procedure that can connect experts with judges. A variety of training courses between judges and medical personnel may be an appropriate solution. 감정에 있어 가장 중요한 요소는 감정인과 이를 활용하는 법관이다. 현재 우리 주위에서 의료와 관련하여 진행되고 있는 감정에 대해 적지 않은 불만의 소리를 들을 수 있다. 이를 해결하기 위해서는 위의 두 요인에 관심을 기울일 필요가 있다. 먼저 감정인의 수준을 높이기 위해서는 감정이라는 법적 절차가 일상적으로 행해지는 의료행위와는 다름을 이해하고, 이를 적절하게 수행할 수 있는 감정인을 선별하여야 하며, 나아가 감정을 공정하게 진행할 수 있는 소송절차를 마련하는 것이 필요하다. 이와 관련하여 감정을 전문으로 진행하는 기관 혹은 법원의 설립을 검토할 필요가 있겠다. 공정하고 수준 높은 감정을 위해서는 합당한 비용이 어느 정도인지도 고민할 필요가 있다. 비용은 감정에 대한 보답일 수 있겠지만 공정성을 담보하고 책임을 묻기 위한 수단일 수도 있다. 절차적으로 감정 결과의 공개나 감정이 재판에 어느 정도 기여하였는지 등을 피드백하는 과정은 감정의 질을 높이는데 기여할 것으로 본다. 감정을 활용하는 법관 또한 감정에 관심을 기울이고 능동적으로 참여할 필요가 있다. 감정이 법에 명시된 절차임을 고려할 때 이를 활용하는 소비자가 적극 참여하지 않는다면 감정의 수준을 높이고자 하는 여러 노력들은 한계에 부딪힐 가능성이 높다. 그리고 절차적으로 반드시 법관이나 법원만이 해결할 수 있는 것들도 적지 않다. 마지막으로 감정이 전문가와 법관을 연결해 주는 절차임을 고려할 때 이들이 항시 교류할 수 있는 장을 마련하는 것은 중요하다. 아마도 전문가는 법조인에게, 그리고 법조인은 의료인들에게 서로에게 필요한 다양한 교육 과정이 이러한 자리이지 않을까 생각한다.
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이숭덕,이윤성,이정빈 대한법의학회 2001 대한법의학회지 Vol.25 No.1
The feasibility of species identification using sequence analysis of the cytochrome B (Cyt B) gene in mitochondrial DNA was investigated DNA was extracted from rune different animals that could be easily met in our surroundings and Cyt B gene was amplified. Direct sequencing results for the amplified PCR products were compared with each other. Human was also included Nucleotide sequence of the Cyt B gene for earth animals was also compared with the previously known ones registered in nucleotide databases, Genebank. The inter-species sequence variation was high as the percent similarity of each sequences ranged 64.6-83.5%. Compared to this the percent similarity of sequences obtained here were high when compared to the sequences of the same species registered in the database showing relatively low intra-species variation. This data shows that the nucleotide sequences of Cyt B gene in t certain biological materials can be identified at species level. The applicability of this method to the forensic field is also demonstrated by performing a casework, determination of the origin for the placentae which were commercial available as "invogorant". Points about the use of Cyt B gene in forensic field was also reviewed.
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서울지역 의과대학에서의 법의부검 : 서울의대에서 과거 5년동안 실시한 부검 경험 보고
이숭덕,이정빈,이윤성 대한법의학회 2000 대한법의학회지 Vol.24 No.1
This is the statistical analysis about the medicolegal autopsy cases performed by the Department of Forensic Medicine, Seoul National University Medical College during the last 5 years. 1.The total number of cases was 330, among which male took 256(77.5%) cases, female 74(22.5%) cases. The natural death took 134(40.6%) cases, unnatural death 177(53.6%) cases. For remaining 19(5.8%) cases the cause of death was known, although thorough analyses including microscopic examination, toxicology study were done. The thirties were most as 25.4% of the total cases and the forties followed the second. 2.The number of autopsy showed severe variation annually or monthly in a year. 3.Among unnatural death, death by violent injury took 87(49.2%) cases, asphyxia 48(27.1%) cases, intoxication 17(9.6%) cases and death by thermal injuries or electric injuries 25(14.1%) cases. 4.Among natural death the abnormality in cardiovascular system were the most as 73(54.5%) cases, gastrointestinal system 38(28.4%) cases and central nervous system 12(8.9%) cases. 5.There were some differences between these and the data from other institute in the ratio of male to female, ratio of natural death to unnatural death and the ratio of specific cause of death among natural and unnatural death. 6.During this work, there were some difficulties in the aspect the irregularity of the work, in requesting toxicology and in the expense needed for the process. Considering the educational aspect for the students and the residents in pathology, performing medicolegal autopsy in medical college should be encouraged. The small number of autopsy and the closeness of the scene made the scene investigation possible in several cases, which is the ideal form that we must consider when we set up our system for the postmortem investigation.
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