한약재 중 아플라톡신 Monitoring

이성득,김연선,윤용태,박애숙,신영,김화순,김유경,최병현 한국약용작물학회 2010 한국약용작물학회지 Vol.18 No.5

Our paper shows the results of 302 samples of herb medicines about fungal contamination at Yakyeang markets in Seoul. The sample medicines were treated VICAM pretreatment and analysed by post column derivatisation procedure(PHRED-HPLC) with a fluorescence detector. Aflatoxin B1 was founded from 50.3% of samples, aflatoxin B2 was 39.7%, aflatoxin G1 was 21.2% and aflatoxin G2 was 23.5%. The detected ranges of aflatoxin B1, B2, G1 and G2 were from 0.1 to 57.2 μg/kg, 0.1 to 42.6 μg/kg, 0.1 to 23.5 μg/kg and 0.1 to 9.5 μg/kg respectively. Among total samples, 26 samples contained aflatoxin B1 violated the regulation (less than 10 ug/kg) for aflatoxin B1 of KFDA. From the result, we could presumed that more than a half of samples were contaminated by aflatoxins. Therefore, it seems to be necessary that the new safety giudeline will be established aflatoxin B2, G1 and G2 from herb medicines as aflatoxin B1.

동물성 생약의 중금속 함량 및 이행률 조사

이성득,이정숙,황광호,김희순,유인실,한기영,채영주 대한약학회 2013 약학회지 Vol.57 No.1

The objective of this study is investigation of contamination levels and transfer rate of heavy metals and assessment of health risk effects in animal medicines. A total of sixteen items with 148 samples were obtained for this investigation from major herbal medicine producing areas and herbal markets from 2011 to 2012. Inductively coupled plasma mass spectrometer method was conducted for the quantitative analysis of Pb, Cd and As. In addition, the mercury analyzer system was conducted for that of Hg without sample digestion. The average contents of heavy metals in animal medicines were as follows : 1.55 mg/kg for Pb, 0.28 mg/kg for Cd, 0.70 mg/kg for As and 0.047 mg/kg for Hg respectively. The average contents of heavy metals in decoctions were as follows : 0.03 mg/kg for Pb, 0.01 mg/kg for Cd, 0.17 mg/kg for As and 0.003 mg/kg for Hg respectively. The average transfer rates of heavy metals from animal medicines to decoctions were 1.9% for Pb, 3.6% for Cd, 24.2% for As and 6.0% for Hg respectively. Cancer risks of animal medicines and decoctions through slope factor by ministry of environment republic korea and environmental protection agency was 1.36×10-6 and 2.50×10-8 for Pb and 7.46×10-5 and 1.98×10-5 for As (assuming that the total arsenic content was equal to the inorganic arsenic) respec- tively. The hazard index of heavy metals in animal edicines and decoctions was below recommended hazard index as 0.52 and 0.05 respectively. Taken together, cancer risks about Pb and hazard indexes of animal medicines and decoctions indicate that the majority of samples except for some samples with relative high contents of heavy metals were safe. But cancer risks about As of animal medicines indicate that the majority of samples were not relatively safe.

빵의 프로피온산 함량 분석방법 개선

이성득,김연선,두옥주,김미선,박영혜,김일영,채영주 한국식품위생안전성학회 2009 한국식품위생안전성학회지 Vol.24 No.4

Most propionic acid is added to food (especially breads) as preservatives and its form is sodium or calcium salt. Most countries admitted propionic acid as food preservatives but a tolerance limit is somewhat different according to countries. Recoveries of the official method for propionates reported as 50.0~60.0%. Accordingly new rapid determination method for propionates was developed using formic acid added sodium chloride (5 g) and ether (formic acid : ether = 1 : 2) as the extraction solvent to improve the official method with the complex processes. Propionate was dissolved from the samples with formic acid omitting steam distillation and ion exchange procedure. Then propionate in formic acid was extracted with ether and sodium chloride again. A 1 μl aliquot of the filtrate of ether was analyzed by gas chromatograph. Recoveries from sample A and B fortified with propionic acid sodium salt were 85.0 % and 90.0 %, respectively.

유통 공정서 미수재 약용식물의 잔류농약 함량 조사

이성득,김욱희,최영희,김남훈,한성희,이현경,김윤희,박혜원,이정미,한기영,채영주 한국약용작물학회 2013 한국약용작물학술대회 발표집 Vol.2013 No.05

유통 환제의 유해 중금속 함량 및 위해도 평가

이성득,이영기,김무상,박석기,김연선,채영주 한국식품위생안전성학회 2012 한국식품위생안전성학회지 Vol.27 No.4

The objective of this study is investigation of contamination levels and assessment of health risk effects of heavy metals in herbal pills. 31 Items and 93 samples were obtained for this investigation from major herbal medicine producing areas, herbal markets and on-line supermarkets from Jan to Jun in 2010. Inductively coupled plasma mass spectrometer method was conducted for the quantitative analysis of Pb, Cd and As. In addition, the mercury analyzer system was conducted for that of Hg without sample digestion. The average contents of heavy metals in samples were as follows : 0.87 mg/kg for Pb, 0.08 mg/kg for Cd, 2.87 mg/kg for As and 0.16 mg/kg for Hg, respectively. In addition, the average contents of heavy metals in different parts of plants, including cortex, fructus, herba,radix, seed, algae and others were 0.63 mg/kg, 3.94 mg/kg, 1.42 mg/kg, 1.05 mg/kg, 0.16 mg/kg, 22.31 mg/kg and 10.17 mg/kg, respectively. After the estimations of dietary exposure, the acceptable daily intake (ADI), the average daily dose (ADD), the provisional tolerable weekly intake (PTWI) and the relative hazard of heavy metals were evaluated. As the results, the relative hazards compared to PTWI in samples were below the recommended standard of JECFA as Pb 3.1%, Cd 0.9%, Hg 0.5%. Cancer risks through slope factor (SF) by Ministry of Environment Republic Korea and Environmental Protection Agency was 4.24 × 10−7 for Pb and 3.38 × 10−4 for As (assuming that the total arsenic content was equal to the inorganic arsenic). Based on our results, possible Pb-induced cancer risks in herbal pills according to parts used including cortex, fructus, herba, radix, seed, algae and others were 1.95 × 10−7, 1.45 × 10−6,2.14 × 10−7, 6.27 × 10−7, 1.99 × 10−8, 3.61 × 10−7 and 9.64 × 10−8, respectively. Possible As-induced cancer risks in herbal pills by parts used including cortex, fructus, herba, radix, seed, algae and others were 1.54 × 10−5, 7.24 × 10−5,1.23 × 10−4, 2.02 × 10−5, 3.25 × 10−6, 2.18 × 10−3 and 5.67 × 10−6 respectively. Taken together, these results indicate that the majority of samples except for some samples with relative high contents of heavy metals were safe.

한약재 중 아플라톡신 분석에 관한 연구

이성득,김연선,김남훈,정희정,정삼주,김화순,김경식,한기영 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.4

The increase in the consumption of herb medicines have made their use a public health problem due to the potential fungal contamination and the risk of the presence of mycotoxins. 360 samples of herb medicines were evaluated for the aflatoxin contamination. The natural occurrence of aflatoxins in these samples were determined using immunoaffinity column clean up and high performance liquid chromatography (HPLC) with post-column derivatization. For samples analyzed, mean levels (incidence) of AFB1, AFB2, AFG1 and AFG2 in positive samples were 1.4 μg/kg (46.4%), 0.4 μg/kg (25.4%), 1.1 μg/kg (37.8%) and 0.9 μg/kg (24.3%), respectively. Recoveries of the full analytical procedure were 71.7~99.7% for AFB1, 88.1~99.2% for AFB2, 82.8~95.5% for AFG1 and 77.9~90.0%for AFG2. The excess cancer risk estimated using the cancer potency of aflatoxin B1 (7 (mg/kg/day)−1 for HBsAg− and 230 (mg/kg/day)−1 for HBsAg+) were 1.30 × 10^(−5) ~ 1.22 × 10^(−7) for hepatits B surface antigen negative (HBsAg−) and 3.31 × 10^(−4)~ 3.12 × 10^(−6) for hepatits B surface antigen positive (HBsAg+) respectively. In conclusion, although the contamination levels of samples used in the study were low, further actions are also required to undertake a program of herbal surveys in order to access mycotoxin contamination overall so that the safety of public will be protected.

유통 한약재의 아플라톡신 오염실태 조사

이성득,이정숙,고숙경,황광호,김희순,김유경,김경식,한기영 대한보건협회 2011 대한보건협회 보건종합학술대회 Vol.2011 No.-

[연구배경] 약용식물은 오래전부터 질병의 예방 및 치료의 목적으로 사용되어 왔으나, 대부분의 약용식물들은 재배에서 가공 소비되는 동안 미생물에 오염될 가능성이 있고, 특히 곰팡이에 의한 아플라톡신의 오염 가능성이 높기 때문에 유통 한약재의 아플라톡신 함량을 조사하는 것이 필요하다. [연구목적] 유통 한약재의 안전성을 확보하기 위하여 2010년 동안 서울지역에서 유통 중인 곰팡이독소(아플라톡신 B1) 기준이 설정된 한약재 규격품에서 아플라톡신의 함량을 조사하였다. [연구방법] 생약 등의 잔류오염물질 기준 및 시험방법에 따라 시료를 수집한 후 면역친화성 칼럼을 이용하여, 시험용액을 조제하였고, 형광검출기와 유도화장치(광화학반응장치)가 부착된 액체크로마토그래프를 이용하여 표준용액과 시험용액의 머무름 시간과 면적을 비교하여 아플라톡신의 함량을 측정하였다. [연구결과] 한약 규격품 19종 360건을 분석한 결과 아플라톡신 B1은 168건(46.4%), 아플라톡신 B2는 92건(25.4%), 아플라톡신 G1은 137건(37.8%) 그리고 아플라톡신 G2는 88건 (24.3%)검출되었고, 검출량은 아플라톡신 B1 1.4 ±1.8 ㎍/㎏, 아플라톡신 B2는 0.4 ±1.1 ㎍/㎏, 아플라톡신 G1은 1.1 ±5.0 ㎍/㎏ 그리고 아플라톡신 G2는 0.9 ±3.4 ㎍/㎏이었으며, 백자인과 빈랑자를 제외한 모든 시료에서 아플라톡신 B1 허용 기준(10 ㎍/㎏ 이하)에 적합하였다.

14각형 헥사아자 거대고리 구리(II)착물과 음이온의 반응에 미치는 리간드의 치환기 효과

姜信杰,李成得성득,權在槿,鄭守炅 大邱大學校附設 基礎科學硏究所 1991 基礎科學硏究 Vol.8 No.-

Reactions of copper(In complexes of 1, 8-dimethyl-, 1, 8-diethyl-, and 1, 8-dipropyl-1, 3, 6, 8, 11, 13-hexaazacyclotertradecanes(A, B, and C, respectively) with halide and pseudohalide ions have been studied in aqueous solutions by spectrophotometric method. It is observed that the equilibrium constants(K) of these reactions decrease with the ligand structure in the order A> B>C for anions and decrase with the anions in the order SCN^(-)>N_(3)^(-)>Br^(-)>Cl^(-) for complexes. The values of K increased with increasing temperature. Thermodynamic parameters obtained from the equilibrium constants are investigated.

유통 공정서 미수재 약용식물의 잔류농약 함량 조사

이성득(Sung-deuk Lee),김욱희(Uk-hee-Kim),최영희(Young-hee Choi),김남훈(Nam-hoon Kim),한성희(Sung-hee-Han),이현경(Hyung-kyung Lee),김윤희(Yoon-hee Kim),박혜원(Hye-won Park),이정미(Jung-mi Lee),한기영(Ki-young Han),채영주(Young-joo Cha 한국약용작물학회 2013 한국약용작물학회 학술대회논문집 Vol.2013 No.1

Transfer Rates of Toxic Metals during Decoction Preparation from Hebal Medicines and Safety Evaluation of the Final Decoction Products

김동규,이성득,유인실,정권,박승국 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2

Transfer rates of toxic metals during herbal decoction preparation were investigated using risk assessment tools. Decoction samples were analyzed using Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) and a mercury analyzer after microwave digestion. The average decoction transfer rate (%) for each toxic metal after boiling was 10.5 for As, 4.3 for Pb, 4.1 for Cd, and 2.7 for Hg. A risk assessment of toxic metals in decoctions was performed based on the percentage provisional tolerable weekly intake (PTWI) values for a mean value scenario and a 95th percentile value scenario. Samples were collected of 68 decoctions representing 8 types frequently prescribed by oriental medicine clinics in Seoul, Korea. Both scenarios demonstrated low PTWI values. All decoction samples were safe according to risk assessment results.