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이동훈,김영준,강현삼,이병재 한국유전학회 1986 Genes & Genomics Vol.8 No.2
The nucleotide sequences of tRNAs were compared and the homologies between a pair of sequences were calculated. The homology is high between phylogenically related organisms, but low between eukaryote and prokaryote. The organelle's tRNA sequence shows very lower homology with chromosomal tRNA sequence. The homology and evolotionary distance was obtained by the method of Kimura (1980) with the sequences of chromosomal tRNAs which have the same anticodon (GUC) to compare orthologous genes. The phylogenic tree was constructed by unweighted pairgroup method using arithmatic average (UPGMA). The time of divergence of prokaryote and eukaryote was estimated to be 6.4×10^(10) years ago and the branching order in this phylogenic tree was in good agreement with that obtained by primary protein structures and 5S rRNA sequences.