Methylelaiophylin의 항균활성

이동선,이상한,우주형,이진만,서영배,홍순덕,Lee, Dong-Sun,Lee, Sang-Han,WOO, Ju-Hyung,Lee, Jin-Man,Seu, Young-Bae,Hong, Soon-Duck 한국생명과학회 1997 생명과학회지 Vol.7 No.3

Methylelaiophylin은 gram양성균에 대하여 광범위한 항균활성을 보이며, 특히Bacillus subtilis에서 superoxide redicals을 발생하여 항균활성을 나타낸다. B. subtilis에 대한 항균활성은 wild type strain보다 mutant strain인 rec$^{-}$에 대하여 더민감하며, 이러한 항균력은 항산화제인 dithiothreito에 의하여 현저히 감소되었다. 또한 항균력은 세포내 RNA합성보다 DN합성에 효과적으로 작용하였다. 이러한 결과들은 methylelaiophylin의 항균활성이 세포내에서 발생한 활성산소에 의하여 기인한 것임을 나타낸다. Methylelaiophylin generated superoxide radicals in Bacillus subtilis and showed antibacterial activity against a broad range of gram positive bacteria. The inhibition of DNA synthesis was more sensitive than one of RNA synthesis. A recombination-deficient mutant strain of B. subtilis was 2-fold more sensitive than a wild strain, and this sensitivity was reduced in the presence of an antioxidant, dithiothreitol. Methylelaiophylin generated superoxide radicals in B. subtilis lysates, and this suggests that the antibacterial activity of methylelaiophylin is related to the generation of active oxygen species in the cells.

기체크로마토그래피 및 질량분석법에 의한 민속 소주중의 알코올 동족체 분석

이동선,박혜성,김건,이택수,노봉수,Lee, Dong Seon,Park, Hye Seong,Kim, Geon,Lee, Taek Su,No, Bong Su 대한화학회 1994 대한화학회지 Vol.38 No.9

전통 민속소주의 향기성분의 실체를 규명할 목적으로 안동소주, 문배술, 이강주, 진도홍주 중에 존재하는 알코올 동족체인 퓨젤유 성분을 GC-FID-MS로 분석하여 중국산 카오량츄인 Moutainchiew, Ergoutoutiu 등과 비교하였다. 다공성 중합체인 Porapak Q를 이용한 고체상 추출법과 수증기 증류법, 용매추출법 등의 시료전처리 방법을 비교 실험하고 분석조건에 따른 알코올 동족체의 용리거동을 검토하였다. 컬럼오븐온도 변화와 분자량, 끓는점, 용량계수 사이에는 직선적 상관성을 나타내었다. 알코올 동족체의 탄소수가 증가할수록 머무름시간, 분자량, 끓는점, 용량계수의 log값들은 직선적으로 증가하였다. 알코올 동족체의 유전상수가 클수록 머무름 시간은 짧아지고 용량계수는 작아지는 경향을 나타내었으나 탄소수 4개의 sec-butyl alcohol은 유전상수가 작음에도 불구하고 탄소수 3개인 n-propyl alcohol보다 머무름시간이 짧았다. Ethyl alcohol 이외에 methyl alcohol, n-propyl alcohol, isobutyl alcohol, isopentyl alcohol, phenethyl alcohol 등이 검출되었다. 중국산 고량주에는 n-propyl alcohol 함량이 상대적으로 많은데 비하여 우리나라 소주에는 isopentyl alcohol의 함량이 높았다. 관능검사보다 객관적인 객관적인 함량형태 인식을 위하여 다변량통계해석법(주성분분석)을 사용하여 고찰한 결과 우리나라 민속소주는 중국산 고량주와는 완전히 다른 형태를 보여주었다. This work is undertaken to determine alcohol homologues so-called fusel oil that may be present in the Korean folk sojues (distilled liquor) made from grains and to describe sample preparation and analytical method by GC-FID-MS. Solid phase extraction method for sample preparation by using porous styrene divinyl benzene polymer (Porapak Q) was compared with steam distillation and solvent extraction method. Retention behaviors of homologous series of alcohols were also studied. Log values of retention time, molecular weight, boiling point, and capacity factor of alcohols showed linear correlations to the carbon number of an alcohol, to the oven temperature, and to the dielectric constant. Components such as methyl alcohol, n-propyl alcohol, isobutyl alcohol, isopentyl alcohol, and phenethyl alcohol have been identified. The more amount of isopentyl alcohol than other alcohols are contained in the Korean folk sojues, while that of n-propyl alcohol are contained in Chinese kaoliangchiew. Degree of similarity or dissimilarity and classification of the individual samples were discussed using multivariate statistical analysis(principal components analysis) based on GC data.

Farmesy-Protein Transferase의 저해제 Dihydrotanshinone l.

이동선,이상한,하상철,김종국,서영배,홍순덕,Lee, Dong-Sun,Lee, Sang-Han,Ha, Sang-Chul,Kim, Jong-Guk,Seu, Young-Bae,Hong, Soon-Duck 한국생명과학회 1998 생명과학회지 Vol.8 No.2

Farmesy-Protein Transferase의 저해제는 Ras단백질의 발암활성을 차잔하는 항암제의 후보로서 알려져있다. 우리는 최근에 topoisomerase I에 대하여 저해활성을 갖는 dihydrotanshinone I을 약용식물인 Salvia miltorhiza Bunge(Danshen)으로부터 분리하였다. Dihydrotanshinone Iml 작용기작의 해석을 위한 시도에서 farmesy-Protein Transferase에 대한 저해능($IC_{50}$치= 15ug/ml)을 관찰하였으며, 이것은 유용한 항암제로서의 가능성을 제시한 결과로 본다. An inhibitor of farnesy-protein transferae is known to be a fgood candidate for antitumor agent that block the oncogenic activity of Ras protein . We recently isolated and characterized dihydrotanshinone I from Salvia miltiorrhiza Bunge (Danshen), an oriental herb, which has an inhibitory activity of toposimerase I to some cancer cell lines. In order to examine the molecular mechanism of dihydrotanshinone I, we studied the farmesy-Protein Transferase activity by dihydrotanshinone I. As a result, we found that result, we found that dihydrotanshinone I showed inhibitory effect on farnesyl-protein transferase with $IC_{50}$ value of 15 ug/ml. This result suggest that dihydrotanshinone I may be an useful anticancer agent with the inhibitory activity of farnesyl-protein transferase.

Inhibition of $\alpha$-Glucosidase Activity by Quercetin

이동선,김종국,이상한,Lee, Dong-Sun,Kim, Jong-Guk,Lee, Sang-Han The Korean Society for Microbiology and Biotechnol 2006 한국미생물·생명공학회지 Vol.34 No.4

Quercetin is a flavonoid molecule that is known to tie in various sources of natural products such as vegetables and fruits. It has been proven that quercetin plays a crucial role in the prevention of colon cancer as well as homeostasis as radical scavenger in human body. It is also well-known that glycosidases, including $\alpha$-glucosidase, are involved in a variety of degenerative metabolic disorders. In the course of screening useful $\alpha$-glucosidase inhibitors, we screened out quercetin as a $\alpha$-glucosidase inhibitor from chemical libraries. Quercetin was shown to be a reversible, slow-binding, and noncompetitive inhibitor of yeast a-glucosidase with a K$_i$ value of $6.3\times10^{-8}$ M when it was included with an enzyme mixture. Together, these results show that quercetin has potential in treating disorders including diabetes, although the further mechanistic study is needed. Quercetin은 flavonoid 계통의 물질로서 자연계에서 채소나 과일에 매우 흔하다. 또한 인체에서는 radical scavenger로서 신체항상성 뿐만 아니라 직장암의 예방에도 중요한 역할을 한다. Glucosidase를 포함한 glycosidase는 많은 종류의 대사성 질환에 관련이 있다. 유용한 $\alpha$-glucosidase 저해제를 스크리닝 하던 중 chemical library로부터 quercetin을 선별하였다. Quercetin은 $\alpha$-glucosidase에 가역적으로, 느리게 결합하며, 비경쟁적인 저해제인데, $6.3\times10^{-8}$ M의 Ki값을 가진다. 이러한 결과는 비록 기전 연구가 더 필요하지만, quercetin이 당뇨병과 같은 퇴행성 질환을 치료할 수 있다는 잠재성을 보여주는 것이다.

A Study on Network Planning and Optimization Strategy for Network Scalability

이동선,Lee, Dong-Sun The Korean Institute of Communications and Informa 2007 韓國通信學會論文誌 Vol.32 No.6a

현재의 전송네트워크용량을 향상시킬때 중요하게 고려해야 할것중의 하나가 네트워크 scalability 이다. 새로운 풀 메쉬의 링 확장 방법 및 플래닝 툴을 제안하였다. 3$\sim$15 노드의 확장링에서 본 툴을 사용하여 SNR이 증진되는 것을 입증하였다. 노드의 출력신호 및 광 SNR이 제안된 NPOT에 의해 -16dBm/10dB 에서 +005dBm/21dB 로 증진되었다. One of the major issues that has to be carefully considered when upgrading current transport network capacity, is network scalability. A novel full-meshed connected ring expansion methodology and planning tool have been proposed. A 3 to 15 node expansion ring has been studied by demonstrating a dramatic system SNR improvement when the proposed planning tool was used. The results are that node output signal and optical SNR have been improved from -16dBm/10dB to +005dBm/21dB by NPOT.

계면중합에 의한 폴리우레아 마이크로캡슐의 제조 -이소시아네이트의 종류에 따른 마이크로캡슐의 생성수율과 열적특성-

이동선,조창기,Lee, Dong-Sun,Cho, Chang-Gi 한국섬유공학회 2002 한국섬유공학회지 Vol.39 No.5

In this study, a series of polyurea microcapsules were prepared by interfacial polyconden-sation using various isocyanates as the oil soluble monomer and diethylenetriamine as the water soluble monomer. Nonionic surfactant, NP-10, was used as an emulsifier. The yields of microcapsules from aliphatic isocyanates were higher than those of microcapsules from aromatic isocyanates due to the flexibility of the aliphatic chains. Polyurea microcapsules obtained from aliphatic isocyanates were stable well above 25$0^{\circ}C$ and showed better thermal stability compared to those obtained from aromatic analogs.

계면중합에 의한 폴리우레아 마이크로캡슐의 제조 -분산상의 점도에 따른 마이크로캡슐의 크기 변화-

이동선,금창헌,조창기,Lee, Dong-Sun,Kum, Chang-Hun,Cho, Chang-Gi 한국섬유공학회 2007 한국섬유공학회지 Vol.44 No.5

Polyurea microcapsules were prepared by interfacial polycondensation using diisocyanates as the oil soluble monomers and diethylenetriamine as the water soluble monomer. NP-10, nonionic surfactant, was used as an emulsifier. Toluene was used as the diluent of the diisocyanates as well as the means to prepare microcapsule with empty shell. Number average particle size of the microcapsule showed a linear relationship with the viscosity of the dispersed oil phase. Polyurea microcapsule with thinner shell thickness showed good thermal stability well above $250^{\circ}C$ and showed same thermal stability compared to that with thicker shell thickness.

LabVIEW^® 기반 6축 수직 다관절 로봇(RS2)의 이종 모션 블랜딩 연구

이동선,정원지,장준호,김만수,Lee, D.S.,Chung, W.J.,Jang, J.H.,Kim, M.S. 한국생산제조학회 2013 한국생산제조학회지 Vol.26 No.1

For fast and accurate motion of 6-axis articulated robot, more noble motion control strategy is needed. In general, the movement strategy of industrial robots can be divided into two kinds, PTP (Point to Point) and CP (Continuous Path). Recently, industrial robots which should be co-worked with machine tools are increasingly needed for performing various jobs, as well as simple handling or welding. Therefore, in order to cope with high-speed handling of the cooperation of industrial robots with machine tools or other devices, CP should be implemented so as to reduce vibration and noise, as well as decreasing operation time. This paper will realize CP motion (especially joint-linear) blending in 3-dimensional space for a 6-axis articulated (lab-manufactured) robot (called as "RS2") by using LabVIEW$^{(R)}$ (6) programming, based on a parametric interpolation. Another small contribution of this paper is the proposal of motion blending simulation technique based on Recurdyn$^{(R)}$ V7 and Solidworks$^{(R)}$, in order to figure out whether the joint-linear blending motion can generate the stable motion of robot in the sense of velocity magnitude at the end-effector of robot or not. In order to evaluate the performance of joint-linear motion blending, simple PTP (i.e., linear-linear) is also physically implemented on RS2. The implementation results of joint-linear motion blending and PTP are compared in terms of vibration magnitude and travel time by using the vibration testing equipment of Medallion of Zonic$^{(R)}$. It can be confirmed verified that the vibration peak of joint-linear motion blending has been reduced to 1/10, compared to that of PTP.

이온 이동도 분석법의 원리와 응용에 관한 고찰

이동선,Lee, Dong-Sun 한국분석과학회 2001 분석과학 Vol.14 No.1

인터뷰 - 신선농산물 포장 설계를 위한 스마트폰 앱 개발

이동선,Lee, Dong-Seon 한국포장협회 2014 包裝界 Vol.257 No.-

신선농산물의 포장 설계를 위한 스마트폰 application(app. 앱)이 개발되어 업계의 주목을 끌고 있다. 이 신선농산물 포장 설계 앱은 경남대학교 식품생명학과 식품포장학연구실이 농림축산식품부 연구비지원 사업인 지능형농식품포장연구센터(주관기관 동국대학교) 과제의 일부로 개발된 것으로 향후, 농산물의 생산자, 포장업계, 유통업계에 광범위하게 사용되는 것은 물론 소비자에게는 신선한 고품질 농산물의 제공할 것으로 기대되고 있다. 이번 앱 개발의 중심에 있는 이동선 교수에게 개발 과정과 함께 식품 포장업계의 현황 및 전망에 대한 견해를 들어보았다.