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Ehrlich복수암 세포의 핵추출액중에 나타나는 U6 RNA의 Uridine 부가 반응에 관계하는 효소의 정제
이도익 中央大學校 遺傳工學硏究所 1992 遺傳工學硏究論集 Vol.5 No.1
In the gene expression, transcription step is one of the most important parts. Recently in the transcription step, many results suggest key process named splicing which regulate pre-mRNA to mRNA in a nucleus. Splicing is regulated accurately by spliceosome which contains snRNAs and snRNPs complex. Previously we reported the uridylation of U6 RNA in a nuclear extract of Ehrlich ascites tumor cells. This reaction required ATP or GTP, although these nucleotides were not incorporated into U6 RNA itself. By chromatography of a nuclear extract of Ehrlich ascites tumor cells on phosphocellulose and DEAE-cellulose and DEAE-sephadex, U6 RNA could be separated from an enzyme adding a uridine residues to this RNA and could get more purified uridylation enzyme fraction higher specific activity than start material.
천연물에서 단리한 식물정제 탄닌의 항암효과 및 생물학적 반응 조절 물질로서의 기능 검색
이도익,조장현,이민원,Lee, Do-Ik,Cho, Jang-Hyun,Lee, Min-Won 대한약학회 1998 약학회지 Vol.42 No.4
Praecoxin A, an ellagitannin, purified from Alnus hirsuta var.microphlla was evaluated on the antitumor activity. Praecoxin A had the significant cytotoxicity to s ix tumor cell lines: human chronic myelogenous leukemia K-562, human promyelocytic leukemia HL-60, mouse leukemia P388, mouse lymphocytic leukemia L-1210, sarcoma-l8O, mouse lymphoma L5178Y except L-1210. And the most sensitive cell line was K-562 ($ED_{50}=2.43{\mu}g/ml$). The $ED_{50} of praecoxin A against HL-60, P388, L-1210, sarcoma7l8O and L5178Y were 6.28, 8.66, 10.00, 7.01, $9.32{\mu}g/ml$, respectively. Praecoxin A showed the increasing effect in life span by 36.8% on the 1st day after treatment of 10mg/kg in mice bearing sarcoma-180 tumor cells (ascitic form) via NCI (National Cancer Institute, U.S.A.) protocol in vivo assay. As a result, praecoxin A is considered to show the antitumor activity.
J774.1 Cell에 있어서 Lectin Binding protein의 동정
이도익 中央大學校 遺傳工學硏究所 1996 遺傳工學硏究論集 Vol.9 No.1
무척추 동물의 생체 방어 기전은 거의 알려져 있지 않으며, 이들의 면역학적인 거의 밝혀진 것이 없는 채로 많은 연구가 시도될려고 하고 있다. 이러한 무척추 동물의 방어 기전을 molecular level 에서 밝히는 것은 아주 중요한 것이라고 할 수 있을 것이다. 일반적으로 lectin은 고등 동물에 있어서 면역계 세포들에 대한 mitogen으로 알려지고 있으며 새롭게 곤충에서 추출된 Sarcophaga lectin이 macrophage를 통한 (i) LDMC반응을 매개로 하여 생체 방어에 중요한 역할을 하는 것으로 인식되어지며, 이러한 것에 논거하여 (ii) lectin binding protein의 동정을 시도하였는 바 이러한 결합 단백질의 동정을 통하여 이러한 반응 기전의 분자생물학적 전달 기전을 밝힐 수 있는 것으로 생각되는 바이다. Sarcophaga lection is a galactose-bind lectin purified from Sarcophaga perguna(flesh fly) larvae. The biological chavacteristics of this lectin are: it is not induced in the normal larvae, but it is promptly induced when the body wall of larvae is injured by a prick with a needle. we studied this lectin's funtion about LDMC(lectin Dependent Macrophage Mediated Cytotoxicity) reaction in 51Cr-labeled MM46 tumor cells incubated with or without glycogen-induced macrophages with the used does of flesh fly lectin. Also We checked lectin binding protein of J774.1 cell's membrane surface.
Sarcophaga peregrina(쉬파리) 유충의 지방체세포 및 배양세포 NIH-Sape-4의 핵추출액에 있어서의 U6 RNA에 대한 Uridine부가반응
이도익,이광표 중앙대학교 약학연구소 1992 약학 논총 Vol.6 No.-
The uridylation of U6 RNA in a nuclear extract of Sacophaga peregrina fat body and NIH-Sape-4 was examined. Nuclear extract of fat body and NIH-Sape-4 contained U6 RNA, but did not have U6 RNA uridylation enzyme activity. In a previous experiment, we found 105bp uridylated U6 RNA in a nuclear ectract of Ehrlich asictes tumor cells, in this experiment we could find new 125 bp uridylated band. Contrary to nuclear extract of mouse Ascites tumor cell, biological meaning of hetero geneous size band(125bp) of Sarcophaga peregrina and NIH-Sape-4 is unknown. More experimental results may suggest important function of new band(125bp).
흰쥐에 있어서 수산화알루미늄 마그네슘 현탄액이 Metronidazole의 약물동력학에 미치는 영향
이도익,장원규,이광표 중앙대학교 약학연구소 1990 약학 논총 Vol.4 No.-
The effect of aluminum-magnesium hydroxide suspension, antacid on the phamackinetics of metronidazole was investigated in female rats. Blood samples were collected at various time intervals for up to 24hr following the oral administration of metronidaz ole (50㎎ / ㎏) alone (clntrol group) or in combination of the antacid (1200㎎ / ㎏) administered concurrently (T_1 group), 1hr later (T_2 group) or 2hr later (T_3 group). Plasma level-time curves were individually fitted to one-compartment open model with first order absorption. In group T_1 in which antacid was orally coadministered with metronidazole, the pharmacokinetic parameters of the drug were significantly in comparison with control studies, Group T_2, in which the dosing interval of antacid was 1hr sgowed that the pharmacokinetic parameters were not profoundly affected except profoundly decreased elimination rate constant and slightly decreased percent absorbed. Group T_3, in which the dosing interval of antacid was 2hr, exhibited no significant changes when compared with control group. In view of the tendency of aluminum-magnesium hydroxide suspension observed in the present study todelay or reduce the absorption of metroridazole, the practitioner should aware of the potential disadvantage in combining with an antacid or concurrent dosing with an antacid. In order to minimize e the GI symptoms associated with metronidazole therapy, it is highly recommended that dosing interval of an antacid should be at least 2hr later following the oral administration of metronidazole.