동력경운기 주요부품의 수명추정에 관한 연구

이대원,김경욱,금동혁 ( Dae Weon Lee,Kyeong Uk Kim,Dong Hyeug Kum ) 한국농업기계학회 1983 바이오시스템공학 Vol.8 No.1

영남지역 벼재배 지대별 비래해충의 실태 조사

이대원,유소영,차욱현,김수빈,송동훈 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

기후변화로 인한 한반도의 온도 상승은 비래해충으로 인한 벼 작물의 피해를 증가시키는 추세이다. 이에 따라토착 가능성이 있는 비래해충에 대한 조사가 필요한 실정이다. 따라서 본 연구에서는 영남지역의 벼 재배지대별비래해충의 실태 조사를 실시하였다. 조사 대상으로는 벼를 가해하는 주요 비래 해충인 멸구류 3종(벼멸구, 애멸구,흰등멸구)과 나방류 2종(혹명나방, 멸강나방)을 선정하였으며, 조사 지역으로는 경남의 고성과 밀양, 경북의 안동,상주로 각 도별 2개의 시를 선택하여 조사를 실시하였다. 멸구류의 경우 육안 조사, 포충망 조사, 끈끈이 트랩및 동력 흡충기를 이용하여 발생수를 조사하였으며, 나방류의 경우 앞서 제시된 방법에 페로몬 트랩을 추가하여조사하였다. 그 결과 멸구류는 조사 지역 전체적으로 꾸준히 발생한 것에 반해, 나방류의 경우 경북은 조사 초반,경남은 조사 후반부터 발생됨을 확인할 수 있었다. 이러한 조사 결과는 기후변화로 인한 벼 작물의 비래해충 피해를감소시키기 위한 조기 경보 시스템 확립에 토대가 될 것이다.

Acaricidal activity of commercialized insecticides against Haemaphysalis longicornis (Acari: Ixodidae) nymphs

이대원,장규식,김민준,안용준,조형찬,김순일 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.4

The acaricial activity of 13 commercialized insecticides in Korea against the hard tick nymphs Haemaphysalis longicornis collected in a field was tested using nymph dipping and folded filter paper methods and a spraying-residual assay. In a nymph dipping bioassay, 100% mortality at all tested doses was observed in insecticides containing active ingredients such as phthalthrin 8% + D-phenothrin 2% + piperonyl butoxide 20%, deltamethrin 1.5%, pyrethrum 0.5%, lambda-cyhalothrin 5.5%, alpha-cypermethrin 10% and teflubenzuron 50%, whereas the insecticide containing etofenprox 5% + octachlorodipropyl ether 11% at recommended dose (RD) and 0.5 times RD showed 97 and 90% activity, respectively. Other insecticides containing alpha-cypermethrin 5%, polyoxyethylene isostearylether 10%, etofenprox 5%, bistrifluron 10% and Bti did not showany significant activity. In the folded filter paper contact testwith nymph H. longicornis at RD, polyoxyethylene isostearylether 10%, deltamethrin 1.5% and alpha-cypermethrin 10% showed 100% mortality at 1 day after treatment (DAT). At 7 DAT, polyoxyethylene isostearylether 10%, deltamethrin 1.5% and lambda-cyhalothrin 5.5% exhibited 100, 93 and 87% mortality. Although the selected 5 insecticides showed strong mortality under laboratory conditions, their residual effect sprayed on field grown lawn leaves significantly decreased depending on DAT. Etofenprox 5% mixture (+octachlorodipropyl ether 11%) only showed 93 and 80% mortality at 1 and 2 DAT, respectively. Polyoxyethylene isostearylether 10% gave 0% mortality at even 1 DAT and the other insecticides also showed 60–70% mortality at 1 DAT. These results indicate that pyrethroid insecticides gave good activity against the H. longicornis nymphs but their residual effect is within 1 DAT under field conditions.

보리의 發生段階의 와 in vitro 에서 leaf thionins의 硏成에 관한 연구

이대원 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

Regeneration of plants from isolated protoplasts of cereals is so far restricted to embryogenic calli and suspension cell cultures as protoplast sources. A possible explanation is that toxic factors are released or produced when starting with differentiated cells. These investigations showed that the leaf thionins released from the cells which are the non regenerable plant material fron the isolated protoplasts of barley. Transcripts for leaf thionins were not found, even after stressing, in developing sexual embryos commonly used for initiating embryogenic in-vitro cultures, or in embryogenic cells yielding regenerable protoplasts. On the other hand, non-regenerable tissues of barley plantlets contained significant amounts of thionin mRNAs or - in the case of leaves grown under high light which resuced the thionin content - wounding induced a strong increase in the concentration of leaf thionin mRNA.

Graphene Oxide Incorporated Antifouling Thin Film Composite Membrane for Application in Desalination and Clean Energy Harvesting Processes

이대원,라즈쿠마 파텔,Lee, Daewon,Patel, Rajkumar The Membrane Society of Korea 2021 멤브레인 Vol.31 No.1

Water supplies are decreasing in comparison to increasing clean water demands. Using nanofiltration is one of the most effective and economical methods to meet the need for clean water. Common methods for desalination are reverse osmosis and nanofiltration. However, pristine membranes lack the essential features which are, stability, economic efficiency, antibacterial and antifouling performances. To enhance the properties of the pristine membranes, graphene oxide (GO) is a promising and widely researched material for thin film composites (TFC) membrane due to their characteristics that help improve the hydrophilicity and anti-fouling properties. Modification of the membrane can be done on different layers. The thin film composite membranes are composed of three different layers, the top filtering active thin polyamide (PA) layer, supporting porous layer, and supporting fabric. Forward osmosis (FO) process is yet another energy efficient desalination process, but its efficiency is affected due to biofouling. Incorporation of GO enhance antibacterial properties leading to reduction of biofilm formation on the membrane surface. Pressure retarded osmosis (PRO) is an excellent process to generate clean energy from sea water and the biofouling of membrane is reduced by introduction of GO into the active layer of the TFC membrane. Different modifications on the membranes are being researched, each modification with its own advantages and disadvantages. In this review, modifications of nanofiltration membranes and their composites, characterization, and performances are discussed. 물 공급은 늘어나는 담수 수요와 다르게 줄어들고 있다. 담수의 수요를 충당하기 위해서 나노여과법은 가장 효율적이고 경제적인 방법이라고 할 수 있다. 해수담수화를 위한 나노여과법의 일반적인 방법으로는 나노여과 멤브레인을 이용한 역삼투압 방식이다. 하지만 기존의 멤브레인들은 주요 특성인 안정성, 경제성, 그리고 살균 및 방오특성이 부족하다. 기존의 나노여과 멤브레인을 향상시키기 위해서 친수성과 방오성이 높은 흑연 산화물이 가장 향상성이 높으며 널리 연구되고 있는 재료이다. 멤브레인 변형은 다른 레이어에 적용될 수 있다. 얇은 막으로 이루어진 멤브레인은 다른 세 레이어로 구성되어 있다, 표면의 폴리아미드 레이어, 기공 레이어, 그리고 전체적인 구조를 구성하는 지원 직물이다. 정삼투압 토한 에너지 효율적인 해수담수화 방식이지만 효율이 생물 오염 때문에 떨어진다. 산화그래핀 결합은 향균 기능을 향상할 수 있으며 멤브레인 표면에 바이오필름 생성을 억제할 수 있다. 압력지연삼투는 해수에서 청정에너지를 발전시키는 최고의 방법 중 하나이다. 멤브레인의 생물 오염은 합성 폴리머 멤브레인의 합성 레이어에 산화 그래핀을 합성하여 줄일 수 있다. 나노여과 멤브레인을 개량하는 여러 연구가 각자의 장단점을 가지고 이루어지고 있다. 이 보고서는 나노여과 멤브레인의 개량, 성질, 그리고 성능에 대해 논의한다.

Reduction of lean NO2 with diesel soot over metal-exchanged ZSM5, perovskite and γ-alumina catalysts

이대원,송승진,이관영 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.2

The catalytic performances of metal-exchanged ZSM5, perovskite and γ-alumina catalysts for the reduction of nitrogen dioxide (NO2) by diesel soot were investigated. The reaction tests were performed through temperatureprogrammed reaction (TPR), in which NO2 and O2 were passed through a fixed bed of catalyst-soot mixture. On the three types of catalyst, NO2 was reduced to N2 by model soot (Printex-U) and most of the soot was converted into CO2. Pt-, Cu- and Co-exchanged ZSM5 catalysts exhibited reduction activities with conversions of NO2 into N2 of about 20%. Among the perovskite catalysts tested, La0.9K0.1FeO3 showed a 32% conversion of NO2 into N2. The catalytic activities of the perovskite catalysts were largely influenced by the number and stability of oxygen vacancies. For the γ-alumina catalyst, the peak reduction activity appeared at a relatively high temperature of around 500 oC, but the NO2reduction was more effective than the NO reduction, in contrast to the results of the ZSM-5 and perovskite catalysts.

弗素의 齒牙우식증 豫防效果에 關한 力學的 調査 및 實驗的 硏究

李大源 최신의학사 1975 最新醫學 Vol.18 No.6

Anti-caries effect of fluorine is well known fact and a lot of researches has been conducted. The objective of present study was to ascertain the relationship between fluoride content in the drinking water, and dental caries prevalence in permanent teeth, and fluorine content in the several layers of human enamel. The areas selected in this study were Chowol-Myun Kyungkee -Do, Sangsoo -Myun Chunrabuk-Do and Sannai-Myun Chunrabuk-Do where the fluorine content in drinking water were 0.2~0.3p.p.m. (call AREA A below). Maegok-Myun and Hwangkeum-Myun Chungchungbuk-Do where the fluorine content in drinking water contain 1.1~1.6 p.p.m. were also selected as an area of comparison (call AREA B below). DMF person rate, the fluorine content in the three layers of enamel, mottled index and occurrence of mottled enamel of the children aged 6 to 11 in both sexes were surveyed. The results was as follows: ① The average DMF person rate of the AREA A showed 46.7% in male, 49.4% in female and 48.1% of both. The content of fluorine in the outer third of the enamel was 420p.p.m. , 95p. p. m. in the middle third and 64p. p. m. in the inner third. The average mottled index was revealed 0. 28 and the mottled enamel occurrence was 31.1%. ②The average DMF person rate of the AREA B showed 25.6% in male, 27.2% in female and 26.4% of both. The content of fluorine in the outer third of enamel was 518 p.p.m. , 226 p. p. m. in the middle third and 137 p. p. m. in the inner third. ③Average DMF person rate of the AREA B revealed lower by 45. 1% than the AREA A.

산탄총에 의한 두경부손상 1례

이대원,김종규,김형진,최득록 대한이비인후과학회 1997 대한이비인후과학회지 두경부외과학 Vol.40 No.11

Identification and biochemical analysis of a novel pectate lyase 3 gene in Bursaphelenchus xylophilus

이대원,강재순,정찬식,한혜림,문일성,박석주,이시혁,고영호 한국응용곤충학회 2013 Journal of Asia-Pacific Entomology Vol.16 No.3

Anovelpectate lyase3(Bx-PEL3) gene from the stage-specifically expressed sequence tag library of Bursaphelenchus xylophilus was cloned. Bx-PEL1 and Bx-PEL2 were relatively predominant to Bx-PEL3 in quantitative real-time PCR analysis. The variation of expression level among Bx-PELs according to different life stages suggests that each Bx-PEL may play different biochemical roles in pathogenesis. Recombinant Bx-PEL3 showed activity against polygalacturonic acid and its optimized physical conditions for pH and Ca2+ concentrations were 9.0 and 0.5 mM, respectively. Homology modeling revealed that Bx-PELs were structurally similar to PEL from the Bacillus strain KSM P-15 and shared essential residues for calcium binding and catalytic activity. Bx-PEL3 was expressed near the esophageal gland as shown in other Bx-PELs, indicating that Bx-PEL3 may be involved in the degradation of the cell wall after secretion from the stylet. Our data suggest that the novel PEL3 gene is a putative pathogenic factor which is biochemically functional.

RNA interference of PBAN receptor suppresses expression of two fatty acid desaturases in female Plutella xylostella

이대원,고영호,김용균 한국응용곤충학회 2011 Journal of Asia-Pacific Entomology Vol.14 No.4

Pheromone biosynthesis-activating neuropeptide (PBAN) stimulates sex pheromone biosynthesis by activating PBAN receptor (PBANr), which triggers a specific signal transduction in the pheromone gland cells. We have shown that RNA interference (RNAi) of PBANr of Plutella xylostella significantly suppressed pheromone biosynthesis and subsequent mating behavior. In order to assess molecular events occurring downstream of PBAN signaling, we cloned partial sequences of Δ9 and Δ11 fatty acid desaturases of P. xylostella. Phylogenetic analysis indicated that these two desaturase genes were highly clustered with other desaturases associated with sex pheromone biosynthesis in other insects. RT-PCR analysis showed that Δ9desaturase was dominantly expressed in adult females, whereas Δ11 desaturase was expressed in all P. xylostella developmental stages. When PBANr expression was suppressed by PBANr-RNAi, the treated females also showed significant suppression of expression of both desaturases. These results suggest that expressions of the two desaturases are controlled by PBAN and that the two desaturases may be involved as downstream components in sex pheromone biosynthesis of P. xylostella.