쉽싸리의 기내 마디배양 시 탄소급원의 종류와 농도가 식물체 재분화 및 증식에 미치는 영향

이나념,김지아,김용욱,김태동 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.2

We examined the effect of carbon sources on the regeneration and ex vitro acclimatization of Lycopus lucidus Turcz. ex Benth. Plantlets were regenerated on the 1/2MS medium supplemented with different concentrations (3 ~ 10%) of sucrose and glucose. The sucrose concentrations of 3% and 5% that were supplied enhanced shoot multiplication and rooting but hampered high concentration growth (including the length of the shoot and root). During ex vitro acclimatization, the tuberization of the root, the root length, the shoot length and the survival rate of Lycopus lucidus plantlets grown using 3% and 5% sucrose were found to be better than the other carbon sources and concentrations. Thus a sucrose concentration of 3% and 5% in the 1/2MS medium appeared to be better for both in vitro growth and ex vitro acclimatization of Lycopus lucidus.

Comparative transcriptome analysis between embryogenic and nonembryogenic callus of Kalopanax septemlobus

이나념,이상아,강민정,주혜준,김지아,박응준 한국산림과학회 2020 Forest Science And Technology Vol.16 No.3

Kalopanax septemlobus is a deciduous medicinal tree species belonging to the family Araliaceae. It contains a number of triterpenoid saponins primarily constituted of hederagenin aglycones. Here, we presented de novo comparative transcriptome analysis using 454 pyrosequencing technology between embryogenic (EC) and nonembryogenic callus (NEC) of K. septemlobus. A total of 372,229 high-quality reads were generated by combining 168,065 EC reads and 204,164 NEC reads, which were then assembled into 80,280 and 63,128 unigenes, respectively. A comparative analysis of transcription profiles showed that 13,766 genes were differentially expressed between EC and NEC. Moreover, functional enrichment analysis revealed that a number of genes involved in the proteolysis and embryogenesis were highly expressed in EC, whereas oxidative stress-responsible genes were overpresented in NEC. Interestingly, the expression levels of genes involved in saponin biosynthesis, such as squalene epoxidase, cytochrome P450s, and glycosyl transferase, were also higher in NEC than in EC, suggesting that their expression is stress-inducible. In this study, we characterized the K. septemlobus transcriptome profiles involved in somatic embryogenesis (SE), which might enhance knowledge of SE process and provide a useful molecular resource for further study of K. septemlobus.

희귀 및 멸종위기 식물 미선나무(Abeliophyllum distichum Nakai)의 절편위치 및 치상방법에 따른 기내증식 및 미세삽목

이나념,김지아,김용욱,최용의,문흥규 한국식물생명공학회 2015 식물생명공학회지 Vol.42 No.3

Using either the apical or axillary bud of the endangered species Abeliophyllum distichum Nakai, we tested the effect of bud position and culture method on shoot proliferation and rooting. In shoot proliferation, the axillary bud explant was more effective than the apical bud and the effect was fostered by BA treatment, whereas no differences were observed in shoot elongation by the explant position. Spontaneous rooting was observed in the MS basal medium and resulted in conspicuous differences in the explant position : more than 80% in apical bud explant and 28% in axillary bud explant was achieved, respectively. The positional effects were also observed in BA pre-treatments: generally vertical culture method appeared to be better in shoot proliferation, growth, and rooting than that of the horizontal culture method regardless of the BA pre-treatment duration. The highest shoot multiplication was achieved through the vertical culture method with axillary bud explant, whereas the best shoot elongation and rooting was obtained using the vertical culture method with the apical bud explant. Apical bud explant was superior to axillary bud explant in ex vitro micro-cuttings and revealed a significant difference in shoot growth and root development. The above results suggest that explant position and culture method influence the efficiency of micropropagation for a rare and endangered plant Abeliophyllum distichum.

쉽싸리의 기내 마디배양 시 탄소급원의 종류와 농도가 식물체 재분화 및 증식에 미치는 영향

이나념,김지아,김용욱,김태동,Lee, Na-Nyum,Kim, Ji Ah,Kim, Yong Wook,Kim, Tae Dong 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.2

본 연구에서는 쉽싸리의 재분화와 순화에 영향을 주는 탄소급원의 효과에 관한 연구를 수행하였다. 식물체 재분화는 1/2MS 배지에 3 ~ 10%의 sucrose와 glucose를 각각 다른 농도로 처리하여 재분화를 유도하였다. 그 결과 3%와 5% sucrose 처리에서 줄기증식과 발근이 증가되었으며, 고농도 처리에서는 줄기와 뿌리 길이 등의 생육이 억제되었다. 기외 순화 중 3% 혹은 5% sucrose 처리 배지에서 생육한 식물체의 뿌리비대, 뿌리길이, 줄기 길이 및 생존율은 glucose 및 다른 농도처리와 비교하여 가장 높게 나타났다. 따라서 3% 및 5% sucrose 처리는 쉽싸리의 기내 생육과 순화를 위해 가장 적정한 농도로 확인되었다. We examined the effect of carbon sources on the regeneration and ex vitro acclimatization of Lycopus lucidus Turcz. ex Benth. Plantlets were regenerated on the 1/2MS medium supplemented with different concentrations (3 ~ 10%) of sucrose and glucose. The sucrose concentrations of 3% and 5% that were supplied enhanced shoot multiplication and rooting but hampered high concentration growth (including the length of the shoot and root). During ex vitro acclimatization, the tuberization of the root, the root length, the shoot length and the survival rate of Lycopus lucidus plantlets grown using 3% and 5% sucrose were found to be better than the other carbon sources and concentrations. Thus a sucrose concentration of 3% and 5% in the 1/2MS medium appeared to be better for both in vitro growth and ex vitro acclimatization of Lycopus lucidus.

윤판나물(Disporum sessile) 아배양에 의한 식물체 재분화에 영향하는 배지 종류 및 생장조절물질 효과

이나념,김지아,김태동,김용욱,Lee, Na-Nyum,Kim, Ji-Ah,Kim, Tae Dong,Kim, Yong Wook 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.1

본 연구는 윤판나물의 효과적인 기내 증식을 위한 배양 최적 조건을 구명하기 위해 수행되었다. 신초 유도 및 생장에 영향하는 배지(MS, B5 및 WPM) 및 절편체 종류에 따른 실험에서 줄기 유도는 액아 절편을 식물생장조절물질 무첨가 MS 배지에 배양 시 절편 당 2.5개로 가장 좋았고, 줄기 신장은 정아 절편을 식물생장조절물질 무첨가 MS 배지 배양 시 7.2 cm로 가장 효과적이었다. BA 침지 처리 시 신초 유도 효과는 액아배양 유래 신초를 BA 침지 무처리구에서 신초 유도(2.29개/절편) 및 신초 신장(7.28 cm)이 가장 효과적이었으며, BA 침지 시간이 길어짐에 따라 신초 유도 및 길이 생장은 감소하였다. 발근에 영향하는 IBA 농도 효과는 정아유래 신초를 1.0 mg/L 처리구에서 배양 시 최대 발근율(100%) 및 뿌리 유도수(21.3개/절편)를 보였으며, 정아 혹은 액아 유래 신초지 종류에 따른 발근율의 차이는 크지 않은 것으로 관찰되었다. 발근된 식물체는 상토로 이식하여 형태적인 변이 없이 정상적인 생장을 보였다. This study was conducted to establish the optimal condition for in vitro propagation of Disporum sessile. In the experiments with different kinds of media (MS, B5 and WPM) and explant types for shoot induction and elongation, the highest number of shoot inductions (2.5/explant) was shown when the axillary bud explants were cultured on MS medium without plant growth regulators (PGRs). The best shoot elongation (7.2 cm) was also obtained when the apical buds were cultured on MS medium. The effect of BA pulse treatments with in vitro shoots was also examined. The highest in shoot induction (2.29/explant) and elongation (7.28 cm) was observed when the shoots from axillary buds were cultured on the media without PGRs. However, it decreased with increasing duration of BA pulse-treatment. The highest rooting rate (100%) and number of root inductions (21.3/explant) were achieved with 1.0 mg/L indole-3-butyric acid (IBA) treatment, whereas no differences were observed by different shoot types. The regenerated in vitro plantlets were hardened and successfully established in soil.

배발생조직 line에 따른 음나무(Kalopanax septemlobus Koidz.)의 체세포배유도, 발아 및 식물체 재생

이나념,문홍규,최용의 한국산림휴양복지학회 2013 한국산림휴양학회 학술발표회 자료집 Vol.2013 No.4

탄소급원에 의한 얌의 기내 비대근 형성과 순화

이나념,김지아,김용욱,김태동 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.2

In this experiment, we investigated the effects of various carbon sources and concentrations on the microtuber induction and acclimatization of the yam (Dioscorea cayenensis). First, the effects of the in vitro carbon sources and concentrations on the microtuber induction were examined. The highest efficiency of the microtuber induction was obtained in the 7% sucrose treatment, whereas the glucose treatment shows no effect on the microtuber formation. Secondly, the effects of the survival rate and the microtuber formation rate after the acclimatization were examined. The diameter (6.1 mm) and fresh weight (0.5g) of the tuberous root are the highest in the pretreatment of the 7% sucrose. Although the survival rate of the pretreatment of the low concentration sucrose (3% sucrose) is 100 %, the growth and development were inhibited. These results suggest the 7% sucrose treatment is appropriate for the yam microtuber formation and acclimatization. In addition, this protocol could be used for the propagation of virus- or disease-free clones and the multiplication of elite yam cultivars.

저온저장 음나무 배발생 캘러스로부터 체세포배 유도와 식물체 재생

이나념,최용의,문흥규 한국식물생명공학회 2015 식물생명공학회지 Vol.42 No.4

Somatic embryogenesis is as an excellent technology for potential use in plant mass production, germplasm conservation, or genetic engineering. We examined the effect of cold storage using 3 embryogenic callus lines with different levels of embryogenesis competence derived from immature zygotic embryo cultures of Kalopanax setemlobus. Somatic embryo induction, germination and plant conversion were evaluated after 1, 3 and 6 months storage at 4°C in the dark. Most cold-stored embryogenic calli formed somatic embryos normally even after 6 months; however, the induction rate was gradually decreased by increasing the storage period. The most competent line tended to show a slight decline in somatic embryo induction rate, as compared with other lines after cold storage. In general, cold storage resulted in reduced somatic embryo germination and plant regeneration, although 93% somatic embryo germination and 91% plant conversion were achieved regardless of the storage period. Cold storage led to cell browning and degradation. Additionally, the cell structures were confirmed by the aceto-carmine and evans blue dye evaluation. Collectively, our results showed that embryogenic callus of K. septemlobus could be preserved at 4°C without subculture for 6 months, and suggested the need for storage of relatively more competent embryogenic calli lines to support somatic embryo induction.

티크의 기내 줄기 생장 및 발근에 미치는 LED (light-emitting diode) 효과

이나념,김지아,김용욱,Lee, Na-Nyum,Kim, Ji-Ah,Kim, Yong-Wook 한국식물생명공학회 2019 식물생명공학회지 Vol.46 No.4

본 연구는 티크의 기내 생장 및 발근에 미치는 light-emitting diode (LED) 효과를 구명하기 위해 수행되었다. 티크의 정아 절편체의 줄기 신장은 LED 혼합광(BR, 50% 청색광 + 50% 적색광) 하에서 DKW 배지 배양 시 3.2 cm로 가장 좋았고, 줄기유도는 광원 및 배지(MS 및 DKW) 효과가 그리 크지 않은 것으로 나타났다. BA 농도 처리 시 최대 줄기 유도는 BR 광원 및 1.0 mg/L 처리구에서 2.4개, 최대 줄기 신장은 BR 및 0.5 mg/L 처리구에서 4.94 cm로 가장 효과적이었다. 발근에 영향하는 IBA 농도 효과는 정아 절편체를 BR 및 0.5 mg/L 처리구에서 배양 시 최대 발근율(93.8%)과 최대 뿌리 길이 신장(1.3 cm)을 보였고, 뿌리 수는 0.2 mg/L (3.1개/절편) 처리구에서 가장 좋은 것으로 나타났다. 발근 식물체는 상토로 이식 후 순화 과정을 거쳐 정상적은 생장을 보였다. This study was conducted to determine the effect of a light-emitting diode (LED) on in vitro shoot growth and rooting in teak (Tectona grandis L.). In the experiments with apical bud explants, the greatest shoot elongation (3.2 cm) occurred when they were cultured on DKW medium under 50% blue and 50% red LED mixture (BR), whereas no differences in growth were observed in different light sources (florescent light [F] or BR) or media (MS or DKW). The highest number of shoot multiplication (2.4/explant) or elongation (4.94 cm) was achieved with 0.5 or 1.0 mg/L 6-Benzyladenine (BA) treatment under BR. In addition, the best rooting rate (93.8%) or root length (1.3 cm) was recorded with 0.5 mg/L indole-3-butyric acid (IBA) treatment under BR, and the highest root induction (3.1/explant) was observed in 0.2 mg/L IBA under BR. The in vitro rooted plantlets were hardened and survived well on soil.

학술연구논문 구두발표 2 : 배발생조직 line에 따른 음나무(Kalopanax septemlobus Koidz.)의 체세포배유도, 발아 및 식물체 재생

이나념,문흥규,최용의 한국임학회 2013 산림과학 공동학술대회 논문집 Vol.2013 No.-