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시설복숭아에서 호박벌(Bombus ignitus Smith)의 화분매개활동 특성
이기열,이상범,안기수,박성규,윤형주,Lee, Ki-Yeol,Lee, Sang-Beom,Ahn, Ki-Su,Park, Seong-Kyu,Yoon, Hyung-Joo 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.2
시설복숭아에서 호박별의 회분매개활동과 효과에 대하여 조사하였다. 2002년 2월 하순경 시설복숭아에서 호박벌의 방화활동은 오전 8시부터 오후 5시까지이며, 활동최성기는 오전 11시였다. 착과율은 호박벌방사구 73.4%, 인공수분구 73.1% 로서 차이가 없었으나, 무처리구는 33.9%로 매우 낮았다. 상품과 율은 호박벌방사구가 90.0% 로서 인공수분구 83.3% 보다 높았으며, 무처리구는 53.8% 로서 매우 낮았다. 호박벌 방사구가 인공수분구에 비해 착과율과 상품과율이 좋았으며, 노동력도 93% 절감되어 호박별 방사에 의한 효과가 뚜렷하였다. The pollinating activity of bumblebee, Bombus ignitus was investigated at the blossom of Peach [Prunus persica (L.) Batsch] planted in vinyl house. The major time of foraging activity by B. ignitus was between 08:00 and 7:00, and the peak time of pollinating activity by B. ignitus was 11 :00 in the peach vinyl house from February 28 to March 5 in 2002. The rate of fruit set and the rate of marketable fruit by foraging activity of B. ignitus were much better than artificial pollination, and the effect of labor reduction by the pollinating activities of B. ignitus released against th ε artificial pollination was took remarkable profits over 93%. Therefore, the foraging activity of B. ignitus in peach vinyl house brought satisfactory results among the rate of fruit set, the rate of marketable fruit and the effect of labor reduction against the artificial pollination.
애반딧불이(Luciola lateralis)에 대한 살충제와 비료의 독성평가
이기열,김영호,이재웅,송명규,남상호,Lee, Ki-Yeol,Kim, Young-Ho,Lee, Jae-Wung,Song, Myung-Kyu,Nam, Sang-Ho 한국응용곤충학회 2008 한국응용곤충학회지 Vol.47 No.3
시판되는 살충제 10종과 비료 6종에 대하여 애반딧불이 각 태별(유충, 성충, 알) 독성평가를 실내실험을 수행하였다. 3령유충에 대한 독성은 MEP, PAP, Assit, Fenthion, Diazinon, Burofezin fenobucarb, Cartap Buprofezin, Thiamethoxam, Fipronil은 80.0%이상 사망율률 보인 반면, IGR(곤충성장 저해제)계통인 Tebufenozide은 33,3%의 낮은 사망률을 보였다. 공시약제인 Assit, Cartap Buprofezin, Fenthion, PAP에 대한 3령 유충의 $LC_{50}$값(반수치사량)은 각각 1.03 ppm, 1.90 ppm, 10.26 ppm, 0.98 ppm이었다. 알에 대한 영향은 매우 높은 독성을 보였다. 반면에 Tebufenozide은 100%의 부화율을 보였다. 공시비료인 요소, 유안, 염화가리, 용성인비, 복합비료(17-21-17), 규산질비료에 유충을 72시간 노출시켰을 경우 각각 27.3%, 56.7%, 73.3%, 0.0%, 0.0%, 0.0%의 독성을 보였다. Toxicity of 10 registered insecticides and 6 fertilizers were tested against $3^{rd}$ larva and adults of Korean firefly, Luciola lateralis Motschulsky(Coleoptera: Lampyridae). All experiments were tested at the recommended concentration of each inescticides by producer. MEP, PAP, Acephate, Fenthion, and Diazinon, which were organophates, a mixtures combined with Burofezin fenobucarb, Cartap buprofezin, and Thiamethoxam(Neonicotinoids), Fipronil(Phenylpyrazoles) showed more 80.0% mortality on larva and adults of L. lateralis. However, tebufenozide(I.G.R) showed low mortality of 33.3%. $LC_{50}$ (ppm) value of Assit, Cartap buprofezin, Fenthion and PAP were showed 1.03 ppm, 1.90 ppm, 10.26 ppm, 0.98 ppm, respectively, against $3^{rd}$ larva of L. lateralis. Effects against eggs showed very high toxicity. Otherwise, tebufenozide(I.G.R) was showed hatchability of 100%. Toxicity of Urea fertilizer, Ammonium sulfate, Potassium chloride, Fused phosphate, Complex fertilizer and Silicate fertilizer were showed the mortality with 27.3%, 56.7%, 73.3%, 0.0%, 0.0%, 0.0%, respectively, when exposed 72 hrs after treatment.
벼먹노린재(Scotinophara lurida)의 월동처와 본답발생
이기열,박성규,안기수,최병렬,Lee Ki-Yeol,Park Sung-Kyu,Ahn Ki-Su,Choi Byeong-Reol 한국응용곤충학회 2004 한국응용곤충학회지 Vol.43 No.4
Overwintering sites and Seasonal occurrence of various growth stage of the rice black bug, Scotinophara lurida BURMEISTER were studied at the rice paddy field in Chungbuk area, from 1999 to 2001. Attraction by light trap from overwintering site of overwintering adults occurred from early June to mid July and its peak was shown on late June. Eggs were oviposited from early July to early August and its peak appeared in late July. Nymphs were observed from mid July to late September with its peak on mid August. The newly eclosed rice black bugs were found in late August and its peak on mid September. The rice black bug overwintered as adult at mountain foot, banks, and rice paddy levee. 충북지역에서 벼먹노린재(Scotinophara lurida)에 대한 월동처와 본논발생에 대하여 1999년부터 2001년에 걸쳐 조사하였다. 벼먹노린재 월동성충은 월동처에서 6월 상순부터 7월 상순까지 유아등에 포획되었으며, 이동최성기는 6월 하순이었다. 본논에서 각 태별 발생 피크는 월동성충이 7월 중순, 알이 7월 하순, 약충은 8월 중순에 가장 높은 밀도를 보였으며, 제 1대 성충은 9월 중순에 가장 높은 밀도 피크를 보였다. 월동태는 성충이며, 월동충은 산기슭, 제방, 논뚝에서 발견되었다.
건선 병변부에서의 가지세포 및 효과기 T세포와 연관된 염증성 싸이토카인 및 케모카인의 발현
이기열 ( Ki Yeol Lee ),전수영 ( Su Young Jeon ),고동엽 ( Dong Yeob Ko ),송기훈 ( Ki Hoon Song ),김기호 ( Ki Ho Kim ) 대한피부과학회 2012 대한피부과학회지 Vol.50 No.7
Background: T cells and dendritic cells (DCs) are more observed in the psoriatic lesion. Inflammatory DCs stimulate T cell differentiation (Th1 or Th17 cells) by producing IL-12 and IL-23 in psoriasis. Th1 expresses CCR5, while CCR6 is expressed by Th17. CCL20, the ligand of CCR6, is expressed mostly in keratinocytes to play an important role in the migration of Th17 cells. Transforming growth factor (TGF)β inhibits the inflammatory cytokines and induces fibrosis. But, there are still controversies about the role of TGFβ1 in psoriasis. Objective: The purpose of this study is to clarify the pathogenesis of psoriasis by comparing the expression patterns of CD11c/IL-23, CCR5/CCR6, CCR6/CCL20, CD11c, IFN-γ, and TGFβ1 among overall lesional assessment (OLA) 1, 3, 5, and the control group. Methods: We performed CD11c/IL-23, CCR5/CCR6, CCR6/CCL20 double-immunofluorecence and immunohistochemistry of CD11c, IFN-γ, and TGFβ1 after classifying the severity of the lesion in 1, 3, 5 with OLA score. Results: As OLA score increased, the infiltration of CD11c+IL-23+ cells, CCR5+ cells, and CCR6+ cells and the expression of CCL20 also showed a significant increase. While IFN-γ+ cells also increased with the OLA score, TGFβ1 showed positivity usually in the increased vascular endothelial cells and the number of TGFβ1+ vascular endothelial cells increased with OLA score. Conclusion: From the results, the interaction among the dendritic cell-effector T cell, and cytokine derived from the cells, and chemokine/chemokine receptor was confirmed to be important in pathogenesis of psoriasis. It was also confirmed that TGFβ1 is not only important at neoangiogenesis, but at inflammatory angiogenesis in psoriasis. (Korean J Dermatol 2012;50(7):599∼608)
건선환자의 말초혈액내 Tc2 세포 및 CCR4의 발현과 PASI Score와의 연관성
이기열 ( Ki Yeol Lee ),이영규 ( Yeong Kyu Lee ),김경희 ( Kyeong Hee Kim ),김기호 ( Ki Ho Kim ) 대한피부과학회 2010 대한피부과학회지 Vol.48 No.11
Background: Psoriasis is a chronic relapsing skin disorder that is characterized by abnormal epidermal proliferation, inflammation and angiogenesis. It causes emotional and social consequences that go far beyond the skin; therefore, many methods to measure and monitor the severity of psoriasis have been reported. Objective: This study aims to evaluate the usability of the flow cytometric analysis of the T cell subsets and their chemokine receptors in the peripheral blood of the psoriasis patients as a severity index. Methods: The T cell subsets and their chemokine receptor expression (CXCR3, CCR4) in the circulating blood of thirty psoriasis patients (PASI score: 2.2∼44.2) and twenty healthy controls were examined by flow cytometry. The relationship between the PASI score and the T cell subsets/chemokine receptors was also analyzed. Results: The patients showed significantly higher number of Tc1 (CD8+CXCR3+), Tc2 (CD8+CCR4+) and CXCR3/CCR4 expressing cells than did the control group. Especially, the moderate to severe patients (a PASI score greater that 5) showed a higher number of Tc1, Tc2 and CCR4 expressing cells than did the control group. In the severe patients (a PASI score greater than 10), the frequency of circulating Tc2 cells and CCR4 expressing cells was directly correlated with the PASI score. Conclusion: Our findings suggest that flow cytometric analysis of the circulating T cell subsets with further classification could serve as an indicator of the disease severity in psoriasis patients. (Korean J Dermatol 2010;48(11):919∼924)