BALB/c 및 NC/Nga 마우스의 아토피성 피부질환모델에서 생약조성물 SPZZC의 치료효능

이금선,아이크,최지영,윤서영,최종현,강태진,오세군,정재훈,Lee, Geum-Seon,Pena, Ike Dela,Choi, Ji-Young,Yoon, Seo-Young,Choi, Jong-Hyun,Kang, Tae-Jin,Oh, Se-Koon,Cheong, Jae-Hoon 대한약학회 2008 약학회지 Vol.52 No.3

The aim of this study was to evaluate if a composition of herb extracts, PLX-PLS was effective to treat atopic dermatitis (AD) in mice. SPZZC is a composition of herb extracts containing the roots of Scopolia parviflora and Paeonia lactiflora, the herb of Zizania caudiflora, the fruit of Ziziphus jujuba and the leaf of Chinese arborvitae. AD in BALB/c mouse was induced by patching ovoalbumin on the backside, while it in NC/Nga mouse was induced by repeated application of 1-chloro 2,4-dinitrobenzene (DNCB). Mice were topically treated with SPZZC or Domohorn ointment on the backside for 2 weeks (BALB/c) or 1 week (NC/Nga). Scratching behavior, clinical skin severity and the levels of WBC, neutrophil, eosinophil and total serum IgE were measured. After AD induction, scores of scratching behavior and clinical skin severity and the levels of WBC, neutrophil, eosinophil and total serum IgE were increased. Treatment with SPZZC significantly decreased scores of scratching behavior and clinical skin severity in a dose dependent manner in NC/Nga and BALB/c mice. Treatment with SPZZC 2% significantly decreased also the levels of WBC, neutrophil, eosinophil and total serum IgE. Especially, treatment of SPZZC 2% reduced more rapidly score of clinical skin severity than clobetasol cream. These results suggest that the SPZZC may be an alternative substance for the management of AD.

NC/Nga 마우스에서 생약 복합 조성물 YJ-1의 아토피성 피부염 치료 효능

이금선(Geum Seon Lee),이기만(Ki Man Lee),심홍(Hong Shim),김진홍(Jin Hong Kim),정재훈(Jae Hoon Cheong),강태진(Tae Jin Kang) 대한약학회 2012 약학회지 Vol.56 No.2

This study was conducted to determine if YJ-1, a novel herbal complex from a mixture of six oriental herbs (Hydnocarpi Semen, Sesami Semen, Dictamni Radicis Cortex, Momordicae Semen, Xanthii Fructus, and Sophorae Radix), has therapeutic properties for the treatment of atopic dermatitis (AD). Using AD like symptom-induced NC/Nga mice by 1-chloro 2,4-dinitrobenzene (DNCB), the effectiveness of YJ-1 on AD was evaluated. Elidel cream?? (1% pimecrolimus) was used as a control. Dermal application of YJ-1 reduced major clinical signs of AD such as erythema, pruritus, lichenification, edema/escoriations, and dryness. Interestingly, YJ-1 more improved AD-related symptoms including decrease of spleen weight, IL-4, and IgE level in the serum as well as reduction of scratching counts and clinical skin severity in the NC/Nga AD mouse model. Especially, treatment of YJ-1 at 20% in NC/Nga mice more effected than Elidel cream. These results suggest that the ointment of YJ-1 may enhance the process of AD healing by alleviating allergic reaction and has potential for therapeutic reagent for the treatment of AD.

검정콩 섭취여부에 따른 일부 대학생의 영양소 섭취상태

이금선 ( Geum-seon Lee ),윤미은 ( Mi-eun Yun ) 대한영양사협회 2021 대한영양사협회 학술지 Vol.27 No.1

This study analyzed the intake of calories and nutrients based on the consumption of black beans. The survey was conducted on 124 college students (male: 42, female: 82), of whom 63.7% (79 students) were consumers of black beans. The calorie intake of the black bean intake group (1599.81±555.48 kcal) was significantly higher than that of the non-black bean intake group (1259.99±507.58 kcal) (P<0.01). The black bean intake group also showed a significantly higher intake of crude protein (P<0.05), plant protein (P<0.01), animal protein (P<0.05), crude fiber (P<0.001), plant calcium (P<0.001), plant iron (P<0.001), zinc (P<0.01), vitamin B₂ (P<0.01), vitamin C (P<0.01), vitamin E (P<0.01) and folic acid (P<0.001) compared to the non-black bean intake group. There was a positive correlation between the frequency of black bean intake and crude fiber (P<0.05), plant calcium (P<0.05), plant iron (P<0.05), and folic acid (P<0.05). Overall, the black bean intake group was more likely to eat black sesame 13.82 times (95% CI=5.37, 35.55), white beans 10.79 times (95% CI=3.53, 33.02), mung beans 7.22 times (95% CI=2.58, 20.23), and brown rice 4.72 times (95% CI=1.88, 11.84), than the non-black bean intake group. In conclusion, we believe that black beans constitute a vital food ingredient that is necessary to provide Korean college students with a well-balanced diet.

혈중 요소질소와 크레아티닌 비율에 근거한 성인 여자의 탈수 여부에 따른 영양소 섭취 정도와 앉아서 보내는 시간과의 관련성: 2016∼2018년 국민건강영양조사를 이용하여

이금선 ( Geum-seon Lee ),김선희 ( Sun-hee Kim ),채수진 ( Su-jin Chae ),윤미은 ( Mi-eun Yun ) 대한영양사협회 2021 대한영양사협회 학술지 Vol.27 No.4

There is a dearth of Korean studies on dehydration, one of the health risks for adult women. This study analyzed the subjects of the 2016∼2018 National Health and Nutrition Examination Survey (KNHANES) to investigate the relationship between nutrient intake ratios and sedentary time in dehydrated women. Body mass index and waist circumference in the dehydrated group (DG) were significantly higher than the normal group (NG). Intake of other liquids in the DG was significantly higher than in the NG, but total water intake in the DG was significantly lower than in the NG. Compared to the 600 sedentary minutes or more per day group, the odds ratio of dehydration was significantly higher in the less than 300 sedentary minutes per day group (1.871 [95% CI: 1.579∼2.215], P<0.001). Compared to the drinking over 6.0 cups of water per day group, the odds ratio of dehydration was significantly higher in the 3 cups or fewer per day (OR [95% CI] is 1.198 times [1.019 to 1.408], P<0.05), and 6.0 cups or fewer group (OR [95% CI] is 1.162 times [1.018 to 1.326], P<0.05). The results of this study showed that the total water intake was relatively lower in the ≥600 sedentary minutes per day group compared to the 300 sedentary minutes per day group, making them more vulnerable to dehydration. The nutrient intake ratios were higher in the dehydration group than in the normal group. In conclusion, emphasis should be placed on the importance of hydration and nutrition education for adult women in the workplace.

음주차수 증가에 따른 장소별 음주량과 알코올 의존

이금선(Geum-Seon Lee),윤미은(Mi-Eun Yun) 한국콘텐츠학회 2021 한국콘텐츠학회논문지 Vol.21 No.2

본 연구의 목적은 다회차 음주에 따른 장소별 음주량과 알코올의존을 분석하는 것이다. 국제 알코올 통제연구의 일환으로 2012년에 수집된 단면 데이터를 사용하였으며, 만19-64세 음주자 1,789명 중 855명을 대상으로 하였다. 다회차 음주자 비율은 남성(χ²=37.607, p<.001), 학생(χ²=52.466, p<.001), 미혼자(χ²=34.205, p<.001), 흡연경험이 있거나 스트레스가 있는 군(χ²=40,09, p<.001, χ²=21.66, p<.001)에서 유의적으로 높았다. 특히, 미혼자, 흡연자, 스트레스군의 알코올 섭취량이 기혼자, 비흡연자, 비스트레스군보다 유의적으로 더 높았다. 다회차에 걸쳐 알코올 섭취량이 가장 높은 장소는 주류 판매 업종인 술집으로 나타났다(F=67.8g, p<0.001). 음주자의 음주차수가 증가할수록 총 알코올 섭취량이 증가하였으며(F=209.993, p<0.001) 알코올의존도 검사인 RAPS4와 DSM-IV에서 양성 반응을 나타낸 음주자의 수(%)가 각각 (F=129.836, p<0.001; F=94.669, p<0.001) 증가하였다. 음주자 중 알코올의존 교차비는 남성이 여성보다 2.3(95% CI: 1.745-3.057)배, 무직자는 직장인보다 3차에서 3.2(95% CI: 1.053-9.838)배, 흡연자는 비흡연자보다 3차에서 3.9(95% CI: 1.230-12.292)배, 그리고 스트레스군도 비스트레스군보다 2.1(95% CI:1.561-2.768)배 높았다. 결론적으로 음주차수가 증가할수록 흡연여부나 스트레스와의 관련성과 알코올 의존도가 높아져 알코올에 더 취약할 수 있음을 시사한다. The purpose of this study was to analyze the alcohol volume consumed and alcohol dependancy according to multiple rounds of drinking. Sectional data collected in 2012 as part of an International Alcohol Control Study were used, and 855 of 1,789 drinker aged 19 to 64 were conducted. The proportion of multiple rounds drinking were significantly higher in males(χ²=37.607, p<.001), students(χ²=52.466, p<.001), single(χ²=34.205, p<.001), smoking experience and stressed(χ²=40,09, p<.001; χ² =21.66, p<.001) among drinkers. In particular, the alcohol volume consumed of unmarried people, smokers, and stress groups were significantly higher than married people, non-smokers and no-stress group. The highest alcohol intake in the first, 2nd and third rounds was found in bars in the liquor trade (F=67.8g, p<0.001). The total alcohol intake increased as the number of rounds increased (F=209.993, p=0.001) and the number of drinkers who tested positive for alcohol dependency increased, using the tests RAPS4 and DSM-IV(F=129.836, p<0.001; F=94.669, p<0.001). The OR of alcohol dependancy in males was 2.3(95% CI: 1.745-3.057), unemployed was 3.2(95% CI: 1.053-9.838), smokers was 3.9(95% CI: 1.230-12.292), and stress group was 2.1(95% CI: 1.561-2.768) compared to females, employed, non-smokers, and no-stress group respectively. In conclusion, it suggests that those consuming multiple rounds drinking can become more vulnerable to alcohol harms due to its relation to smoking, stress and increased dependence on alcohol.

노린재동충하초의 배양 최적화 및 NO 생성 저해 효과

이기만,이금선,남성희,임성실,강태진,Lee, Ki-Man,Lee, Geum-Seon,Nam, Sung-Hee,Lim, Sung-Cil,Kang, Tae-Jin 한국응용곤충학회 2011 한국응용곤충학회지 Vol.50 No.4

동충하초는 예로부터 아시아권에서 한방약재로 사용되어 온 곤충병원성진균이다. 이 중 노린재동충하초(Cordyceps nutans)는 자연 상태에서 비교적 많이 발견되나 이에 대한 연구가 미미한 편이다. 따라서 본 실험에서는 C. nutans 균사체의 최적 배양 조건을 확립하고 배양액 처치 시대식세포의 NO (nitric oxide) 생성 억제 효능을 조사하였다. 균사 생육 적정 온도는 $25^{\circ}C$이었으며 pH는 7.0~8.0 사이의 중성범위로 조사되었다. MCM (mushroom complete medium), V8A (V8 juice agar), YMD (yeast malt dextrose) 배지에서는 균사 생육이 우수하였으나 MMM (mushroom minimal medium) 배지의 경우 균사 생육이 이루어지지 않아 영양원이 필수적이었다. 영양원 선발에 있어 탄소원은 dextrose와 sucrose가 적합하였고 질소원은 ammonium citrate가 균사 생장에 적합하였다. RAW 264.7 세포에 대한 C. nutans 배양액의 세포 독성은 나타나지 않았으며 LPS (lipopolysaccharide)를 처리 한 세포의 NO 생성량은 농도 의존적으로 줄어들었다. 따라서 본 실험 결과는 C. nutans 배양 시 다량의 균사체를 확보할 수 있는 최적 조건을 제공할 뿐 아니라 C. nutans의 항염 관련 우수한 생리 활성이 있음을 보여준다. Cordyceps (vegetable wasp and plant worm), an entomopathogenic fungi, has been used as a herbal medicine in Asian countries since ancient times. Cordyceps nutans is common but there is little research on this species. This study investigated the optimal culture conditions of C. nutans and the inhibitory effect on nitric oxide (NO) production in RAW 264.7 cell treated culture broth. The optimal conditions for the mycelial growth were $25^{\circ}C$ and pH 7.0-8.0. Mycelial growth was highest on mushroom complete medium (MCM), V8 juice agar (V8A), and yeast malt dextrose (YMD) medium. Mycelial growth on mushroom minimal medium (MMM) did not occur, so nutrient source was essential. Dextrose and sucrose as carbon sources, and ammonium citrate as a nitrogen source were satisfactory for mycelial growth. Cytotoxicity of C. nutans culture broth was not found in RAW 264.7 cells. C. nutans culture broth suppressed NO production of lipopolysaccharide (LPS)-stimulated RAW 264.7 cell in a dose-dependent manner. Thus, our results provided the optimal conditions for cultivation of C. nutans and showed that C. nutans may have excellent physiological activities.

단보 : 제주긴뿌리동충하초와 풍뎅이동충하초 배양액의 Nitric Oxide 생성 저해

이기만 ( Ki Man Lee ),이금선 ( Geum Seon Lee ),심홍 ( Hong Shim ),남성희 ( Sung Hee Nam ),강태진 ( Tae Jin Kang ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.1

During search for novel bioactive materials from natural resources with the potential as health food and alternative medicine, the culture broth of Cordyceps longissima (CL) J106, J144 and C. scarabaeicola (CS) J94, J123 were prepared, and their effect on cytotoxicity and nitric oxide (NO) production in RAW 264.7 cells were investigated. Whereas the culture broth of CL J144 and CS J123 had cytotoxicity in RAW 264.7 cells, that of CL J106 and CS J94 did not. The culture broth of CL J106 and CS J94 suppressed NO production in RAW 264.7 cells activated with lipopolysaccharide (LPS) at a dose-dependent manner. These results suggest that culture broth, a by-product of Cordyceps, may have active compounds with anti-inflammatory effect. In addition, it appears that their biological activity is dependent on the strains in spite of the same species.

치자의 스트레스 관련 생리 활성 : 홍삼과의 비교연구

고홍숙(Hong Sook Ko),이금선(Geum Seon Lee),블랜딜(Blendyl Saguan Tan Lee),박형근(Hyun Geun Park),유구용(Gu Young Yoo),임동술(Dong Sool Yim),정인경(In Kyung Jung),오세관(Sei Kwan Oh),정재훈(Jae Hoon Cheong) 대한약학회 2005 약학회지 Vol.49 No.4

Gardenia Jasminoides (GJ) is traditionally used for treatment of hepatic disease, insomnia, anxiety? and inflammatory disease. The aim of this study is to examine effects of GJ extract in response to stress. Animals of the normal group were not exposed to any stress and the control group were exposed to stress. The rats of the Ginseng and GJ supplementary group were orally administered once a day with 100 mg of red ginseng extract, 100 mg of GJ extract/kg body weight. The mice were given water containing 200 mg of red ginseng extract, 200 mg of GJ extract/100 ㎖ potable water. Animals were given supplements for 7 days without stress, and then were given supplements for 5 days with restraint and electroshock stress. After loading final stress, we examined stress related behavioral changes of experimental animals and measured the levels of blood corticosterone. GJ-supplementation partially blocked the stress effect on locomotion and elevated plus maze test in rats, and also partially blocked stress-induced behavioral changes such as freezing, burrowing, face-washing, smelling and rearing behavior in rats. The effect was almost equipotent to Ginseng's effect. GJ-supplementation didn't influence on fatigue related behavior or physical stress resistance. GJ-supplementation decreased the levels of blood corticosterone which is increased by stress in rats. These results suggest that GJ protects partially the living organism from stress attack and it has the potential to be used as a functional material to alleviate stress response.

건강검진자의 당뇨 여부에 따른 혈청삼투압과 크레아티닌과의 관련성

박미경(Mi-Kyung Park),이금선(Geum-Seon Lee),김선희(Sun-Hee Kim),윤미은(Mi-Eun Yun) 한국지역사회생활과학회 2022 한국지역사회생활과학회지 Vol.33 No.2

사회적 음주제공자에 따른 고등학생의 음주량과 음주빈도

김선희(Sun-Hee Kim),윤미은(Mi-Eun Yun),이금선(Geum-Seon Lee) 한국콘텐츠학회 2021 한국콘텐츠학회논문지 Vol.21 No.11

본 연구의 목적은 사회적 공급에 따른 고등학생의 음주량과 음주빈도를 분석하는 것이다. 2012년에 개발된 IAC(International Alcohol Control) Study의 설문지를 사용한 전국 21개 고등학교 16∼19세 음주자 161명의 자료를 분석하였다. 고등학생에게 알코올음료를 제공하는 사회적 음주제공자의 수가 많을수록 회 당 알코올 섭취량이 유의적으로 증가하였다(사회적 음주공급 1인: 59.433 g, 2인: 113.40 g, 3인 이상: 133.56 g). 한편, 사회적 음주제공자 없이 혼자 음주를 하는 그룹인 혼술족의 알코올 섭취량은 167.84 g으로 사회적 음주 제공을 받는 그룹보다도 높게 나타났다. 사회적 음주제공자로 아버지(29.3%)에 의한 음주 제공이 가장 높았고, 친구(25.0%)와 어머니(20.7%)가 주요 음주 제공자로 나타났다. 특히, 아버지(β=-.32, t=3.55, p<.01)와 어머니(β=.22, t=2.71, p<.01)로 인한 음주 제공은 청소년의 사회적 음주 제공 빈도를 증가시키는 요인으로 통계적 유의미성을 나타냈다. 반면, 파트너/남⋅여친구(β=-.23, t=-2.73, p<.01)는 음주 제공 빈도를 감소시키는 요인으로 나타났다. 청소년의 총 알코올섭취량을 증가시키는 요인은 친구(β=.24, t=3.02, p<.01)와 선후배(β=.16, t=2.04, p<.05)의 음주 제공 빈도의 증가로 나타났다. 이는 부모가 사회적 음주 제공자로서 친구와 선후배와 함께 고등학생의 알코올 섭취량을 증가시키는 요인임을 고려하여 알코올 폐해 교육이 학생에서 학부모로 확대되어야함을 시사한다. The purpose of this study was to identify the amount of alcohol and drinking frequency among high school students based on social alcohol drinking supply. The data was on 161 drinkers aged between 16 and 19 from 21 high schools across the country using a questionnaire of the International Alcohol Control(IAC) Study, which was developed in 2012. Results show that the higher the number of social suppliers offering alcoholic beverages to high school students, the greater the consumption of alcohol per episode(59.433 g for one person, 113.40 g for two, and 133.56 g for three or more people). On the other hand, alcohol consumption among ‘Honsul’ people, a group that drinks alone without a social drinking supplier, was 167.84 grams, higher than that of groups that receive social drinking services. As a social drinking supplier for teenagers, drinking was the highest by their father (29.3 %), while friends (25.0 %) and mothers (20.7 %) were the main drinking suppliers. In particular, the provision of drinking due to father(β=-.32, t=3.55, p<.01) and mother(β=.22, t=2.71, p<.01) showed statistical significance as a factor in increasing the frequency of providing social drinking in adolescents. On the other hand, partner/boy or girl friend (β=-.23, t=-2.73, p<.01) was a factor in reducing the frequency of alcohol provision. Friends(β=.24, t=3.02, p<.01) and senior-junior schoolmates(β=.16, t=2.04, p<.05) were the factors that increase the total alcohol intake of adolescents. This is due to the increase in the frequency of alcohol provision. This suggests that alcohol harm education should be expanded from students to parents, considering the role of parents as a social drinking supplier and the link between high alcohol intake among teenagers due to senior-junior friends and schoolmates.