Nuclear polyhedrosis virus 의 polyhedrin 아미노산 및 polyhedrin gene 염시서열 분석

이근광 한국어병학회 1995 한국어병학회지 Vol.8 No.1

H. cunea nuclear polyhedrosis virus (HcNPV) 의 polyhedrin 아미노산 및 polyhedrin gene 의 염기서열을 분석하였다. Polyhedrin 은 SDS-PAGE 상에서 3 개의 polypeptide band 가 나타났고 주요 polypeptide 는 약 25 Kd 의 분자량을 갖고 있었다. 또한 polyhedrin 은 17 개의 다른 아미노산으로 구성되어 있었다. HcNPV DNA 를 EcoRI 효소로 절단하여 α ^(32)P 로 labelling 된 Autographa californica (AcNPV) polyhedrin gene cDNA 의 probe DNA 를 이용하여 hybridization 한 결과 polyhedrin gene 은 EcoRI 절편들중 H 절편에 양성반응을 나타냈다. 또한 polyhedrin gene 을 포함하고 있는 EcoRI-H 절편을 pUC8 벡터에 cloning 한 다음 이를 hPE-H라고 이름하였다. HcNPV genome DNA 의 promoter 부위를 sequence 한 결과 TATA box 의 염기배열은 polyhedrin gene 전사 개시위치로부터 위쪽으로 -79 bp 의 5' flanking 부위에서 발견되었다. polyhedrin gene 내 CAAT box 는 TATA box 측면 염기 배열에서 나타나지 않았고, 4 개의 tandem repeat 5'-CTAATAT-3' 와 5'-TAAATAA-3'의 염기는 polyhedrin gene내 전이 개시 위치로 부터 위쪽으로 -141 과 -108 bp 또는 -83 bp 부위에 존재하였으며, 다른 하나는 전이 개시위치로 부터 아래쪽으로 -52 bp 부위에서 발견되었다. 그리고 polyhedrin gene 내 전이 개시위치로 부터 위쪽으로 -141 bp 부위는 다량의 AT (78%) 염기가 존재하였다. 또한 polyhedrin 의 개시 coding region 은 ATG 였고 종결 coding region은 TAA 였다. The amino acid analysis of polyhedrin protein and nucleotide sequence of polyhedrin gene in H. cunea nuclear polyhedrosis virus (HcNPV) genome have been studied. Polyhedrin had three polypeptide bands in SDS - polyactylamide gel electrophoresis. The major polypeptide had a molecular weight of 25 kd. The polyhedrin was composed of 17 different amino acids. HcNPV DNA was digested with EcoRI restriction enzyme and hybridized with (α ^(32)P) -labelled AcNPV polyhedrin gene cDNA. The polyhedrin gene was located on the fragment of EcoRI-H. The EcoRI - H fragment containing polyhedrin gene was cloned into the EcoRI site of pUC8 vector which was confirmed with southern blotting, and the recombinant plasmid containg polyhedrin gene was designated as hPE-H. The promoter region of polyhedrin genomic DNA was sequenced. The sequences identified as the TATA box was found at the 5' flanking region of the polyhedrin genomic DNA approximately -79 bp upstream from the transcriptional start site. But CAAT-like box was not shown near the TATA-like box in the polyhedrin gene. Four tandem repeats with the sequence 5' -CTAATAT-3' and 5' -TAAATAA-3' were found between -141 and -108 or -83 upstream and -52 bp downstream from the translation start site. About -141 bp region upstream from the translational start site was highly AT (78%) rich. The coding region for the polyhedrin starts and ends with ATG and TAA, respectively.

Hc nuclear polyhedrosis virus DNA 제한효소절편의 molecular cloning 과 외래 유전자 발현

이근광 한국어병학회 1995 한국어병학회지 Vol.8 No.1

HcNPV DNA genome 을 제한효소 EcoRI 으로 절단하여 그들의 일부 절편을 pUC8 vector 에 cloning 한 후 E. coli JM 83 세포에 형질 전환시켰다. 이 결과 24 개의 EcoRI 절편중 12 개의 절편이 cloning 되었다. 이들 제조합체중 4 개는 eNP-O, eNP-Q, eNP-R, eNP-S 라 명명하였다. 또한 이들 제조합체의 외래 유전자 발현을 SDS-PAGE 에 의해 단백질 패턴을 분석하였다. 그 결과 제조합체 eNP-O, eNP-Q, eNP-R 에서는 E. coli JM 83 숙주세포의 단백질 밴드와 비교하여 다른 분자량을 갖는 밴드가 나타났다. Hc nuclear polyhedrosis virus DNA genome was digested with EcoRI endonuclease, these partial fragments were recombined into the pUC8 plasmid vector and transformed in E. coli JM 83 cell. The genome DNA has 24 EcoRI fragments and 12 fragments of them were subcloned. The four recombinants were named as eNP-O, eNP-Q, eNP-R and eNP-S. The expression of foregin gene of the recombinants was investigated by analysing protein patterns on the SDS-PAGE. The eNP-O, eNP-Q and eNP-R were expressed a different weight of protein as comparision with potypeptide bands of E. coli JM 83 host cell.

미용인들의 기호식품에 관한 연구

이근광,송연숙,이향란 대한피부미용학회 2006 대한피부미용학회지 Vol.4 No.2

In order to understand favorite foods of beauty artists, we obtained the results like the following this, by surveying 494 questionnaires(68 men and 426 women) and analyzing the survey using SPSS 10.0.  Smokers are 29.1% among beauty artists; depending on job rank, smokers are 57.1% among managers, 38.5% among staffs, and 38.1% among associate designers. Daily average number of cigarettes is from three to five with the highest frequency (8.9%), from one to two and from five to ten with 5.3% respectively, more than a box of cigarettes with even 4.0%. According to a survey on drinking, beauty artists who drink alcohols are in 85.5%: depending on job rank, staffs with 96.2% and managers with 92.9%. Based on the amount of drinking Soju, 1 ~ 2 glasses is most frequent with 29.1%, 3 ~ 4 glasses is second most frequent with 19.4%, and 5 ~ 6 classes and 1 bottle are with 13.0%, respectively. The reason of drinking is 41.5% with other reasons, 14.6% with stress, and 12.1% with a habit. Among people who drink tea or beverage, 45.7% drink coffee, and 32.4% drink green tea. The reasons of drinking a beverage or tea are resting with 45.1% and habit with 28.7%.

뱀장어 아기미 울혈증에 관한 연구

이근광,김영길,박성우,최민순 한국어병학회 1994 한국어병학회지 Vol.7 No.2

뱀장어의 울혈증 발병 원인구명을 위하여 서해안 지역 5 개 양만장을 대상으로 아가미 울혈증 발병현황과 수질등 역학조사를 실시하였고, 한편으로는 스트레스(handling 과 수온차) 및 병어에서 분리한 아가미 마쇄액을 건강어의 복강에 주사하여 울혈증 발병률과 CHSE-214 에 접종하여 세포변성을 조사하였다. 조사한 양만장의 아가미 울혈증 발병률은 30-80%로서 사육시의 수온과 선별시의 수온차가 클수록 발병률이 높았으며, 수질환경과 본증의 발병과는 뚜렷한 상관관계가 없었다. 또한 발병률이 높은 양만장의 뱀장어는 Ht, Hb, Tp, Alb, Mg 및 Glu 치등이 낮은 반면 GOT, GPT, Ca 및 Met-Hb 치등은 높게 나타나는 경향을 보였으나, 반드시 아가미 울혈증에 비례하여 증감되지는 않았다. 스트레스(handling 과 수온차)를 가함에 따라 아가미 울혈증이 실험적으로 유발되어졌으며, 발병률은 50-70% 이었다. 이러한 스트레스 조건하의 어류의 혈액학적인 성상은 울혈증 발병빈도에 비례하여 Ht, GOT, GPT, Met-Hb, Alb 및 Glu 치등은 증가되었으나, Tp, Hb 및 Mg 은 감소하는 경향을 보였으며, 온도차가 클수록 cortisol 량이 증가되는 경향을 보였다. 그러나, 병어의 아가미 마쇄액을 복강주사 및 CHSE-214 에 접종하였던 바 각각 울혈증의 발병 및 세포변성이 전혀 관찰되지 않았다. 이러한 결과는 선별시 handling 과 수온의 급변이 혈중 cortisol 을 상승시켜 혈액의 전해질 변화를 초래케하므로써 혈액의 흐름을 완만하게 되고 동정맥계로의 혈액유입량이 증가하게 되어 중심정맥동이 확장되므로써 울혈이 형성되는 것임을 시사한다. In order to elucidate the outbreak mechanisms of a new disease which is characterized by an intense congestion in central venus sinuses(CVS) of gill filaments in cultured eel. these experiments were carried out; epidemically surveyed on the cultured eel farms in the vicinity of Kunsan city and experimentaliy outbreaked the disease in the stressful condition such as thermal and handling shock and innoculated the supernatant from the homogenate of naturally severe congested gill into eels and onto the monolayer of the CHSE-214. Although the frequency of congestion in eels of B, C, D and E farms were higher than in eels of A farms, the water qualities (stocked and cultured water) among farms were not a great difference. In eels of B, C, D and E farms, the values of haematocrit (Ht), haemoglobin (Hb), total protein (Tp), albumin (Alb), glucose (Glu), magnesium (Mg) were lower and the values of calcium(Ca), methemoglobin(Met-Hb), glutamic pyruvic transminase (GPT), glutamic oxalacetic transminase (GOT) higher than in eels of A farms. These valules have not related to the frequency of congestion. An intensive congestion and dilataton in CVS of gill filaments in experimentally handling-stressed eels produced similar histopathological changes to those observed in the spontaneously diseased eel, but not in eels experimentally injected with filtering contents. The cytopathic effect on the CHSE-214 was not observed. In stressed eels the congestion of gill was increased in relation to either the decrease ranges of water temperature or the incerase in accllimated times. And increase in Ht, Met-Hb, Alb, Glu, GOT and GPT and decrease in Mg, Hb and Tp were found, which had a close relationship to congestion of gill. Cortisol were increased according to the decrease ranges in acclimated water temperature. From these results, decrease in water temperature during selection placed eels upon the stressed condition, made increase in ionic strength in blood stream, and CVS was dilatated owing to the increased blood inflow.

소나무 뿌리에 서식하는 Ectomycorrhiza의 점유율에 관한 연구

이근광,안태근,오창근,이민웅 동국대학교 대학원 1989 大學院硏究論集-東國大學校 大學院 Vol.19 No.-

The infection rate of Ectomycorrhiza inhabit pine tree roots distributed in three districts of Chung Nam Province was investigated. The results are as follows : Ectomycorrhiza showed a higher infection rate in sunny land than in shady land, and began to inhabit about in middle of April in shady land but began to inhabit late in March in sunny land. After Ectomycorrhiza comes out, it gradually increases. The infection rate of Ectomycorrhiza in the drainage soil is comparatively higher than that in the wet soil. In comparison with the infection rate in fertile and sterile soil, Ectomycorrhiza inhabited more thickly and the hypae expanded more densily in the sterile soil with seasonal minerals when in the fertile soil. The seasonal infection rate of Ectomycorrhiza in pine roots was greatly apparent from late May to early July. After that, it has gone on decreasing, and has disappeared completly from December to March of the next year.

어병 세균에 대한 쑥 정유의 항균력 검색

이근광,김영길,노범진 한국어병학회 1994 한국어병학회지 Vol.7 No.2

어병 세균에 대해 쑥(Artemisia princeps var. orientalis) 추출물인 정유의 항균성을 조사하였다. Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas sorbia, Edwardsiella tarda 와 Streptococcus sp. (yellowtail)는 1,000∼2,000 ppm 에서 증식이 억제되었다. 즉 억제 농도는 A.salmonicida 가 1,000 ppm A.hydrophila, A. sorbia, E. tarda 와 Streptococcus sp. (yellowtail)는 1,500 ppm 이었다. 그러나 Vibrio anguillarum, Vibrio ordalii, Edwardsiella ictaluri 와 Streptococcus sp. (SF-1)는 100∼2,000 ppm 농도에서 현저한 억제 효과는 없었다. The antimicrobial activity of Artemisia princeps var. orientalis essential oil against a partial fish pathogenic bacteria was examined. The growth of Aeromonas hydrophila, Aeromonas salomonicida, Aeromonas sorbia, Edwardsiella tarda and Streptococcus sp. (yellowtail) were inhibited at concentrations of 1,000 to 2,000 ppm. The A. salmonicida was inhibited at 1,000 ppm, A. hydrophila, A. sorbia, E, tarda and Streptococcus sp. (yellowtail) at 1,500 ppm, but Vibrio anguillarum, Vibrio ordalii, Edwardsiella ictaluri and Streptococcus sp. (SF 1) were grown on 100-2,000 ppm.

자외선 조사로 인한 피부 상피암세포 (A431) 의 세포사멸 과정

이근광,최근희,진종언 한국미용학회 2000 한국미용학회지 Vol.6 No.2

A variety of lesions such as burn, keratosis, elastosis, neoplasm can be induced by external physical factors like ionizing radiation, ultraviolet light(UV), and heat. A among them, UV induce a kind of the apoptosis something like differencial phenomenon in late phase which is shown from the keratinization of skin in the general cells. We observed that the special shapes of the apoptosis which is separated from the morphology, biochemical differences by the result of irradiation. Though the cell will be eliminated at the last process of the apoptosis, the mechanism of the apoptosis is not yet clear. So, we have investigated serial procedures something like signal transfer inducing, appearance of the program, acting the apoptosis, and observation on the elimination of dead cells the each mechanism how they related with the apoptosis with the relating factors like hormone and cytokine by irradiation on the skin cells. First, to know the optimum radiation doses and time scales for inducing apoptosis after the eposure to UVC which is degraded to several doses. Second, the transfer of signal inducing the apoptosis will be achieved at the outer and inner part of cells. First of all, the changes of hormone and cytokine which were exists inner part of cells were observed. Third, the program on the apoptosis at the signal arrived cells will be appeared. As it is a general fact that gene is related on each biochemical steps, the general process on the apoptosis inducing mechanism. If the apoptosis out of skin cancer cells and the mechanism will be observed at the gene level by the irradiation methods, it will be the epochal development for the treatment of cancer. And also the delay of skin cells aging and alopecia will be a possible byproduct.

측백나무 (Thuja orientalis) 와 편백나무 (Chamaecyparis obtusa) 정유 (Essential oil) 의 항균력 검색

이근광 한국미용학회 1999 한국미용학회지 Vol.5 No.2

The antimicrobial activities of Thuja orientalis and Chamaecyparis obtusa essential oils against some pathogenic bacteria were examined. Essential oil of T. orientalis showed the best inhibitory effect against V. ordalii ATCC33509, E. tarda ECK-1 and E. tarda KBF-1 at the concentration above 300ppm, and Aeromonas hydrophila ATCC14715, A. hydrophila CF-2, A. salomonisida ATCC14174, A. salmonicida EL-1, Streptococcus sp. and Streptococcus SF-1 at 500ppm, respectively. But V. angulaillum ATCC19264 and the gram positive bacteria(Streptococcus sp.) were relatively insensitive. Also, addition of C. obtusa essential oil against A. hydrophila CF-2, A. salomonisida ATCC14174, E. tardy ECK-1, Streptococcus sp. and Streptococcus SF-1 showed a good inhibitory egect as the concentration increased. But A. hydrophila ATCC 14174, A. salmonicida EL-1, E. tarla KBF-1, Y. angulaillum ATCC19264, V. ordalii ATCC33509 and gram positive bacteria (Streptococcus sp.) were somewhat resistant.

짱뚱어 , Boleophthalmus pectinirostris(Linnaeus)의 간근육조직내(肝筋肉組織內)단백질 전기영동(電氣泳動)분석 및 단백질 , RNA 함량 변화(含量 變化)

이근광,이민웅,정의영,최수경,오영남 한국어류학회 1992 韓國魚類學會誌 Vol.4 No.1

앞서 보고한 雌性 짱뚱어 肝組織의 蛋白質 電氣泳動分析 및 蛋白質, 核酸의 함량 변화 조사에 이어서 雄性 짱뚱어, Boleophthalmus pectinirostris(Linnaeus)의 性成熟에 따른 간조직과 雄性 및 雌性 짱뚱어의 筋肉組織內 蛋白質 電氣永動分析과 定量 및 RNA 含量 變化를 조사하였다. 간과 근육을 이루고 있는 단백질을 SDS-PAGE 분석한 결과 雄性 짱뚱어의 肝組織을 이루고 있는 단백질은 대개 분자량이 15.8∼101.5kd 사이에 존재하였고, 전체 19개의 밴드가 나타났다. 雄性 및 雌性 짱뚱어의 근육 조직을 이루고 있는 단백질 밴드도 거의 동일하며 분자량이 대개 16.9∼116.5kd 사이에 존재하였다. 전체 23개의 밴드가 나타났다. 또한 雄性 짱뚱어의 간조직에 있어서, 단백질 함량은 정소의 成熟期인 6월에 최고값을 나타냈으며, 放精期인 7월과 退化期인 8월에는 정차로 감소하는 경향을 보였다. 근육조직에서는 雌·雄性 모두 6월에 최고값을 나타내었으나, 그후 점차 감소하여 7월에 최저값을 나타내었다가 8월에는 약간 증가하는 경향을 보였다. 雄性 짱뚱어의 간조직과 雄性 및 雌性 짱뚱어의 근육조직의 총 RNA 함량은 成熟期(6월)에 최고값을 나타내었고, 産卵期(7月) 및 退化期(8月)로 되면서 점차 감소하였다. The electrophoretic analysis, variations of protein and nucleic acid contents with sexual maturation, of the liver tissue of male, and of the muscle tissue of male and female B. pectinirostris(Linnaeus) were investigated. As the result of protein analysis of the liver and muscle tissue of male by the SDS-PAGE electrophoretic analysis, the protein of the liver tissue of male was presented 15.8-101.5kd in molecular weight, and appeared 19 bands in all. The protein bands of the muscle tissues of male and female were almost same, their bands were presented 16.9-116.5kd in molecular weight, and appeared 23 bands in all. And in the liver tissue, the protein contents of the liver tissue of male appeared the maximum in June(the mature stage) and afterwards decreased gradually in July(the spawning stage) and August(the degenerative stage). In the muscle tissues of male and female protein contents were shown the maximum in June, and afterwards decreased gradually, the minimum in July, increased a little in August. The total RNA contents of the liver tissue of male, and their contents of the muscle tissue of male and female appeared the maximum in June(the mature stage), and afterwards decreased gradually in July(the spawning stage) and August(the degenerative stage).

무지개송어의 바이러스성 출혈성 패혈증에 관한 기초적 연구

이근광 한국어병학회 1994 한국어병학회지 Vol.7 No.1

무지개송어(Salmo gairdneri)의 바이러스성 출혈성 패혈증에 관하여 연구하였다. 병어의 hematocrit값은 정상어보다 매우 낮게 나타났으며, GOT와 GPT값은 정상어보다 병어에서 약간 높게 나타났다. 병어의 혈청과 장기의 마쇄액을 CHSE-214세포에 감염시켜 세포변성 효과를 조사한 결과 감염 24시간 후에 세포변성이 나타났다. The viral hemorrhagic septicemia of rainbow trout. Salmo gairdneri was studied. The hematocrit values of diseased fish were very low than those of normal fish. And, the GOT and GPT values of serum of diseased fish were a little high than those of normal fish. Cytopathic effect of viral agents(serum and organs of diseased fish) was observed with inverted phase contrast microscopy. After 24hrs infection. the cells were showed the cytopathic effect.