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Mouse에서의 Deoxynivalenol이 면역글로브린에 미치는 영향
이국천 ( Kuk Cheon Lee ),이주홍 ( Ju Hong Lee ),손성기 ( Sung Gi Son ),주영국 ( Young Kuk Joo ) 한국가축위생학회 1992 韓國家畜衛生學會誌 Vol.15 No.2
Mice were fed semi-purified diets containing 0, 2, 10 and 25 ppm(mg/kg) deoxynivalenol over 8 weeks and were assessed for effects on bodyweight gain, serum immunolgiobulin levels and surface immunoglobulin bearing lymphocyte ratio. 1. The rate of body-weight gain was significantly reduced (p<0.05) at the 10 and 25 ppm of DON, whereas the mice ingesting the diet containing 2 ppm DON was not. 2. IgA in serum immunolgiobulin was significantly increased (P<0.05) at the 10 and 25 ppm of DON, but IgG, IgM were decreased, whereas exposure to 2 ppm DON was not change. 3. Concentration of IgA from Peyer`s patch of mice fed DON exhibited increased at 10, 25 ppm. 4. Lymphocytes surface marker studies revealed that IgA, IgG and IgM were 2.2%, 0.4% and 1.5% respectively. These results suggest that dietary exposure to DON alters regulation of IgA production.
건유(乾乳)와 필유(泌乳)에 따른 유우(乳牛)의 혈액(血液) 및 혈액화학상(血液化學像)의 변화에 관한 연구
강정부,이국천,이순선,조희택,안동원,Kang, Chung-boo,Lee, Kuk-cheon,Lee, Soon-sun,Cho, Hee-taek,Ahn, Dong-won 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.4
This experiment was carried out to investigate the hematological and blood chemical values in dairy cattle during the dry and lactating period. Blood was collected from six healthy dairy cattle in Gyeongnam province. Leucocytes count, hemoglobin concentrations and the percentage of packed cell volume were lowest at the early lactation period. Leucocytes count was not affected during dry the to lactating period. The differential count of eosinophilic leucocytes was low at the late lactation period, while the basophilic leucocytes was high at the dry period. Inorganic phosphorus value was below under normal level. Creatinine value was from 1 to 2mg/100ml of serum. Positive reaction to CRP was shown in normal dairy cattle. The values of AST and ALT were higher during the dry than lactating period. r-GTP and total cholesterol tend to be decreased during the lactating period. CPK value was not affected for dry and lactating period.
조각자(주엽) 나무의 생리활성물질 Gleditschia이 비육돈육의 이화학적 성상에 미치는 영향
서종립,허정호,정명호,조명희,이국천,김국헌,하대식,류재두,김충희,김곤섭,김의경,김종수,Seo, Jong-Lip,Heo, Jung-Ho,Jung, Myung-Ho,Cho, Myung-Heui,Lee, Kuk-Cheon,Kim, Kuk-Hun,Hah, Dae-Shik,Ryu, Jae-Doo,Kim, Chung-Hui,Kim, Gon-Sup,Kim, Eui-Gy 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4
The effects of dietary Gleditschia on fatty acid composition, lipid oxidation, and pork quality were investigated. Pigs (n=40) were fed a diet containing 0, 0.1, 0.2, 0.4, and 1% Gleditschia for 14 weeks and slaughtered at 110 kg average. The longissimus throracis et lumborum muscle was collected at 24 hr postmortem. Pork loin chops (3 cm thick) were packaged aerobically and stored at $4^{\circ}C$ for 7 days. Samples were analyzed for fatty acid composition, ultimate pH, thiobarbituric acid-reactive substance (TBARS), color ($L^*$, $a^*$, $b^*$), drip loss and water-holding capacity. There was no significant difference of moisture and crude fat percent in between treatment and control group, and the color stability of pork loin better in all treat groups was more improved during cold storage. The change of pH was significantly increased (p<0.05) in 0.2, 0.4, 1% treatment groups compare to the control group. Water-holding capacity of pig loins was significantly higher (p<0.05) in all treat groups than in control group; 0.2% treat group was the highest in the water holding capacity followed by 0.4, 1, and 0.1% treat groups. Less drip loss of pig loin was observed with samples from Gleditschia-fed pigs except 1% treat group. Unsaturated fatty acid were tend to be decreased and saturated fatty acid were tend to be increased in Gleditschia-treated group. Thiobarbituric acid-reactive substance value of control was significantly higher than that of the Gleditschia-fed group (p<0.05).
허정호 ( Jung Ho Heo ),이국천 ( Kuk Cheon Lee ),조명희 ( Myung Heui Cho ),김국헌 ( Kuk Hun Kim ),하대식 ( Dae Sik Hah ),김종수 ( Jong Shu Kim ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.3
To investigate serum antibody titers of Newcastle disease(ND) virus, serum samples were collected from 19,774 slaughtered chickens from 862 farms in Geoje, Goseong, Tongyung and other province during the period from January 2001 to December 2005. The results obtained are summarized as follows; 1. The negative percentage of ND antibody titer with below 1.0 levels decreased from 46.7% in 2001 to 40.5% in 2004 but those of it increased to 47.0% in 2005 and the positive percentage of ND antibody titer with higher 5 levels increased from 15.8% in 2001 to 26.2% in 2005. 2. The season levels of ND antibody titer showed various levels according to seasons but the general trend of ND antibody titer decreased in the later half of the year than those of the first half of the year. 3. The negative percentage of ND antibody titer on provinces decreased from 52.0%(2001) to 36.2%(2004) in Goseong but those of it increased to 51.4% in 2005. The negative percentage of ND antibody titer of Tongyung and other provinces increased from 51.0%, 28.5%(2001) to 55.0% and 44.3%(2005), respectively. 4. The year average levels of ND antibody titer increased from 2.2 to 2.6 during 2001-2004 but those of it decreased to 2.4 in 2005. The provinces average levels of ND antibody titer increased from 2.0 to 2.8 during 2001-2005 and others provinces except Gyeongnam decreased from 3.3 to 2.6 during the 2001-2005. 5. The farm average levels of ND antibody titer with below 1.0 levels decreased from 26.3% in 2001 to 10.2% in 2004 but those of it increased to 17.6% in 2005 and those of below 1.0 or 2.0 levels increased from 17.9%(2001) to 27.7%(2005). The protective levels of ND antibody titer with higher 5.0 levels increased from 2.1% in 2001 to 8.8% in 2005.
독사 교상후 발생한 전신적 합병증 - 혈액학적 합병증과 신경학적 합병증을 중심으로 -
박은정,윤상규,안정환,최상천,김기운,민영기,이국종,정호성,정윤석,Park, Eun-Jung,Yoon, Sang-Kyu,Ahn, Jung-Hwan,Choi, Sang-Cheon,Kim, Gi-Woon,Min, Young-Gi,Lee, Kuk-Jong,Jung, Ho-Sung,Jung, Yoon-Seok 대한임상독성학회 2009 대한임상독성학회지 Vol.7 No.2
Purpose: This study explored and evaluated the systemic complications resulting from the bite of Korean venomous snake, focussing on hematologic and neurologic features. Methods: Medical records (demographic data, clinical measurements including laboratory results, severity score, and amount of antidote administration, and hospitalization course) of consecutive patients who presented with snakebites to two university teaching hospital during a 10-year period were retrospectively reviewed. Subgroup analysis was conducted for evaluations of anti-acetylcholine esterase administration in complicated victims. Results: The 170 patients displayed occurrence rates of hematologic and neurologic complications of 12.9% and 20.6%, respectively. Among 22 patients with hematologic complications, isolated thrombocytopenia was evident in eight patients (36.4%), prothrombin time (PT) / activated partial thromboplastin time (aPTT) prolongation in 11 patients (50.0%), and both in three patients (13.6%). The mean time to recovery was $4.5{\pm}1.8$ days for isolated thrombocytopenia, and $5.1{\pm}1.8$ days for PT and aPTT prolongation. Hematologic complications could occur suddenly 1?4 days after hospitalization. Among 35 patients with neurologic complications, dizziness was evident in 16 patients (45.7%), and diplopia / blurred vision in 19 patients (54.3%). The mean time to recovery was $3.4{\pm}0.6$ days in patients receiving anti-acetylcholine esterase and $6.9{\pm}1.8$ days in those not receiving anti-acetylcholine esterase (p=0.00). Conclusion: Occurrence rates of hematologic and neurologic complications following venomous snake bite differed as compared to other studies conducted in Korea. Onset of hematologic complications can occur rapidly days after admittance. Anti-acetylcholine esterase administration may be effective in treating neurologic complications.
서득록 ( Deuk Lok Seo ),이국천 ( Kuk Cheon Lee ),이순선 ( Soon Sun Lee ),김종섭 ( Chong Sup Kim ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.1
The morphological development of lungs in fetuses of 60, 90 and 120 days of gestation and neonates of Korean native goats was investigated by light, scanning and transmission electron mictroscope. The results were summarized as follows; 1. Gross findings: In the 60-days-old fetus, the lung was developed and differentiated into six lobes. 2. Light microscopic findings: The gland-like bronchioles were formed in loose mesenchyme at 60 days of gestation and the bronchial wall contained smooth muscles. The loose mesenchyme had been replaced by compact parenchymal tissue at 90 days of gestation and the cartilage plates appeared in bronchial wall which contained blood vessels, submucosal glands and smooth muscles. The lung parenchyma consisted of a fine network of alveoli at 120 days of gestation and the bronchial wall contained well-developed blood vessels, submucosal glands, cartilage plates and smooth muscles. In neonates, the lung tissue was similar to the mature lung tissue and the bronchial wall contained well developed cartilage plates. 3. Scanning electron microscopic findings: The epithelial cells lining the tubules were composed of cuboidal or columnar at 60 days of gestation and the epithelial cells lining the large airways were often ciliated; some were covered with stubby microvilli. The epithelial cells lining the canals were cuboidal at 90 days of gestation and the epithelial cells lining the bronchioles were ciliated cells or nonciliated(clara) cells, The clara cells contained row microvilli. The alvealor development of this stage was rapidly progressed; the subdivision of canals by alveolar crests and assosiated wall attenuation resulted alveoli at 120 days of gestation and the respiratory bronchioles were lined by ciliated or nonciliated epithelial cells. In neonates, the epithelial cells lining the alveolar walls were mainly covered with pneumocyte type Ⅰ; Some were covered with pneumocyte type Ⅱ. 4. Transmission electron microscopic findings: The epithelial cells lining the tubules were adhered with tight junction at apical borders of the adjacent cells at 60 days of gestation, which contained few organells and glycogen. The epithelial cells lining the canals were composed mostly of cuboidal cell at 90 days of gestation and the epithelial cells lining of the bronchioles were ciliated of nonciliated cell, which contained few organelles and abundant glycogen. The epithelial cells lining the alveolar walls were composed of pneumocyte type I and a few pneumocyte type Ⅱ at 120 days of gestation. The epithelial cells lining of the bronchioles were ciliated or nonciliated cells. In neonates, pneumocyte type I was observed as flat and thin cytoplasmic extension in shape. Otherwise, pneumocyte type Ⅱ was observed as cuboidal type with apical microvilli and contained osmiophillic lamellar inclusion bodies. Putting these various experiment results together, the lung development was slowly progressed at early stage, which was rapidly progressed in the late stage of gestation.
경남(慶南) 북부지역(北部地域)에서 도축(屠畜)된 한우(韓牛)의 간질(肝蛭) 감염율(感染率)과 혈액성장(血液性壯)의 변화(變化)에 관한 연구(硏究)
서득록 ( Deuk Lok Seo ),이국천 ( Kuk Cheon Lee ),이순선 ( Soon Sun Lee ),김종수 ( Jong Shu Kim ),김충희 ( Chung Hee Kim ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.2
An abattoir survey was carried out on 300 Korean native cattles reared and slaughtered in North Kyeongnam area to determine the prevalence of Fasciola hepatica infections. The average prevalence of fascioliasis was 46.4%; the infection rate had a tendency to increase with age. Some hematological and biochemical indices were assayed in Korean native cattle naturally harbouring Fasciola hepatica infection and compared with uninfection control. Affected animal revealed reduction total erythrocyte count and homoglobin, which was consistent in total leucocyte count and packed cell volume. Infected cattle had lower level of serum total protein and albumin, was consistent serum calcium and glucose indices, had higher levels of sGOT, sGPT and choresterol indices.
경남 남부지방에서 사육 중인 송아지의 설사병에 관한 역학적 연구
허정호 ( Jung Ho Heo ),조명희 ( Myung Heui Cho ),이국천 ( Kuk Cheon Lee ),박미남 ( Mi Nam Park ),조은정 ( Eun Jeong Cho ),최만수 ( Man Su Choi ),김충희 ( Chung Hui Kim ),강정부 ( Joung Boo Kang ),김의경 ( Eui Kyung Kim ),김종수 ( 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.3
The present study was conducted to investigate the epidemiological characteristics such as isolation rate and antimicrobial drug susceptibility of etiological agents. The specimens (stool or intestine) were collected from 319 calves with clinical diarrhea from 195 farms in Gyeongnam south area (Gosung, Tongyung, Hadong) from June 2005 to August 2006. The isolation rate of Salmonella spp was higher in summer (8.4%) than in winter (4.8%) and the average was 7.2% (23/319 head). Some of Salmonella spp isolated were resistant to penicillin, oxytetracycline, tetracycline, and cephalexin (>90%), but some of them were suscep-tible to norfloxacin, ciprofloxacin, sulfamethoxazole/trimethoprim and amikacin(>30%). There was no statistical difference in the isolation rate of Eimeria spp between summer(48.9%) and winter(42.3%). For the evaluation of infection level of Eimeria spp oocyst per gram of feces(OPG) was examined, and severe, moderate and light infection level were 11.9%, 12.5% and 22.3%, respectively. In the isolation rates of Eimeria spp the calves under 19 days was lowerthan those over 60 days, but there was not different among herd size.
경남 남부지방에서 송아지설사병 원인체 바이러스 검출 조사
허정호 ( Jung Ho Heo ),조명희 ( Myung Heui Cho ),이국천 ( Kuk Cheon Lee ),박미남 ( Mi Nam Park ),조은정 ( Eun Jeong Cho ),최만수 ( Man Su Choi ),김충희 ( Chung Hui Kim ),강정부 ( Joung Boo Kang ),김의경 ( Eui Kyung Kim ),김종수 ( 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.3
Among calves` diseases, digestive diseases most frequently occur in Korea, and especially viral diarrhea is one of the most important diseases. This study was conducted to get some basic information for the control of the viral diarrhea in calves. The samples were obtained from 319 calves with clinical diarrhea from 195 farms in Gyeongnam south area (Gosung, Tongyung, Hadong) from June 2005 to August 2006. Viral detection was examined by polymerase chain reaction (PCR). Etiological viruses were detected from 171(53.6%) of 319 calves, and bovine rotavirus (BRV) were 130 (40.8%) and bovine viral diarrhea virus (BVDV) 41 (12.9%), and no coronavirus was confirmed. Statistical difference was found in BRV detection between summer (32.6%) and winter (57.7%). However there was no seasonal difference in BVDV. In detection rate of the calves under 19days, BRV was highest (55.1%), but BVDV lowest (5.1%). No big difference was in rate among herd size. However, BRV was lowest (26.8%) in the group over 51 heads, but BVDV was highest (19.5%) in the same.
경남지역 내 돼지에서의 swine influenza virus (H1N1, H3N2) 감염률 조사
장은희 ( Eun Hee Jang ),하도윤 ( Do Yun Hah ),박동엽 ( Dong Yeop Park ),이국천 ( Kuk Cheon Lee ),허정호 ( Jung Ho Heo ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.3
Swine influenza is an acute respiratory disease prevalent in pig-growing areas all around the world and plays the roles of an intermediate host to be transmitted to mammals including human beings through a genetic recombination with the avian influenza virus. Recognizing that people could be contracted with swine influenza, this study set out to investigate the seroprevalence of individual and multiple infections with two subtypes (H1N1 and H3N2) of the swine influenza virus in pig farms in the Gyeongnam region according to age, area, and season, as well as to provide basic data for the prevention and control of swine influenza. Used in the study were total 904 swine sera that were not vaccinated against the influenza gathered from the pig farms in the Gyeongnam region from November, 2009 to October, 2010. HerdChek SIV (H1N1, H3N2) ELISA kit (IDEXX Laboratories, USA) was used for antibody testing against swine influenza. The test results show that 370 sera (40.9%) were infected with either H1N1 or H3N2 with 37.3% (337 sera) being contracted with H1N1, 13.1% (118 sera) with H3N2, and 9.4% (85) with both H1N1 and H3N2.