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      • 질산칼슘 처리농도에 따른 수축견사의 이화학적 특성

        이광길,이용우,박영환,남중희 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.1

        This study was carried out in order to find out the relationship between physical and chemical properties of silk fiber treated by concentrated calcium nitrate solution. The tensile, thermal and dynamic mechanical properties are also examined on Ca(NO3) 2, treated silk fibers. The tensile properties of silk fibers treated by calcium nitrate changed with a concentration. The thermal behavior were also affected by the concentration of calcium nitrate. The degradation temperature(endotherms) and glass transition temperature shifted to lower temperature as the treated concentration increased. It is thought that the physical properties are strongly related to the structure and morphology of Ca(NO3)2, treated silk fibers. As a result, these give property changes with a concentration dependence.

      • 곤충유래 키틴/키토산의 구조특성

        이광길,이용우,권해용,여주홍,박인규,남진,설광열 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2

        The chitin was isolated from various kinds of insects such as exuvia of Psacothea hilaris Pascoe, silkworm pupa, Agrius convolvuli or from cuticle of cockroach by treatment with dilute HCl and NaOH. The chemical and crystalline structure was characterized by FT-lR and X-ray diffractometer. All of the chitins extracted from insects showed characteristic α-chitin peaks at the Brags angle 2θ=9.3°, 19.4°, and 23.5° by X-ray diffraction analysis. The transition from chitin to chitosan was confirmed by IR spectra and the degree of deacetylation of the crab shell, silkworm pupa, cockroach, and Psacothea hilaris Pascoe was 70.9, 76.4, 75.5, and 74.1% , respectively. The double diffraction peaks of insect chitosan were observed at 2θ =10° and 20, indicating the characteristic of hydrated crystalline structure of chitosan.

      • KCI등재
      • 질산칼슘 처리 농도에 따른 수출견사의 구조특성

        이광길,이용우,박영환,남종희 한국잠사학회 1997 한국잠사곤충학회지 Vol.39 No.2

        The IR crystallinity index of Calcium nitrate treated silk fiber decreased proportionally to the concentration of calcium nitrate. A partial change of conformation was observed in the concentration of over 46.4~47.6% changing from ß-sheet or to random coil in the crystalline region. This is in coincidence with the result of crystallinity index, which was started to be reduced in the concentration range of46.4~47.6%. A same trend was observed for the X-ray order factor, birefringence, degree of orientation and surface structure. These structural parameters were remarkably changed on the treatment of silk fibers with concentration of 46.4%~47.6% calcium nitrate. Therefore, it seems that there exists a critical concentration of calcium nitrate in affecting the structure and morphology of silk fibers. Acrording to the examination of surface morphology, the fine stripe was observed in the direction of fiber axis at 46.4% concentration. However, the treated concentration was exceeded by 47.6%, the cracks were appeared severely on the fiber surface in the transverse direction as well as fiber axis direction. This result might be related to the tensile properties, specially a tenacity of silk fibers. As a result of quantitative analysis analysis of a dilute acid hydrolysis, three diffirent regions, which are known as a amorphous, semi-crystalline and crystalline region, could be obsteained. The hydrolysis rate curves were different with various concentrations of treatment and the relative contents of each region could be calculated.

      • KCI등재

        Seasonal festival 이 가지는 集團治療的 意義

        李光吉 大韓神經精神醫學會 1974 신경정신의학 Vol.13 No.4

        I analyzed the observations, recordings and psychological data derived from the participation to the seasonal festival in national mental hospital as a therapist, with the result as follows. 1. Enjoyment of play pleasurably. 2. Experience of pain-pleasure principle especially in preparing festival, that is rehearsal. 3. Elevation of sense of reality and objectivity in mutual activity and cooperation. 4. Self-realization through contest and cooperation. 5. Fastening of solidarity linking common identity with aim for freeing from psychic disturbance. 6. Perceiving sense of thankfulness and humour sense.

      • 한국인 악성임파종의 조직학적 및 면역조직학적 연구

        이광길,양우익,이유복 연세대학교 대학원 1985 延世論叢 Vol.21 No.5

        Abstract Various designations uses in malignant lymphomas lacked precise definition and many investigators had uses different terms for the came tumor. As a result of this imprecise terminology, no meaningful clinicopathologic correlations could be eatablished. Rappaport classification was based purely on the cellular morphology and growth pattern of the lymphomas. It was widely accepted, not only because it was relatively easy to apply, but also because it was clinically useful in the design of treatment protocols and in predicting response to therapy and survival of patients. However, based on the new knowledge regarding the nature and origin of the lymphoid cells in malignant lymptomas, many investigators attempted to correct the scientific inaccuracies of the Rappaport classification by proposing new classifications. Lukes and Collins, as well as Lennert et al., placed great emphasis on the fact that the non-Hodgkin's lymphomas were tutors of B- or T-lymphocytes, and they asserted that immunologic identification of lymphoid ceils is an integral part of the diagnosis and classification of the lymphomas. The existence of many classifications not only has resulted in confusion and controversy, but also has made it impossible to compare effectively the results of clinical studios utilizing different systems. To resolve this, the National Cancer Institute of U.S.A. has suggested a new Working Formulation(WF) for Clinical Usage. Relatively recently immunologic methods suck as immunofluorescent and immunoperoxidase staining were popularly used in the diagnosis and stuffy of lymptoproliferative diseases. We performed this stuffy to reclassify the malignant lymphomas according to WF baaed on the histologic features and immunologic phenotypes and to compare the usability and advantages of various immunohistologic staining methods. The material consisted of 53 crises of malignant lymphomas examined at the Department of Pathology, Yonsei University College of Medicine from May, 1983 through December, 1984. All crises were subjected to histopathological analysis, review of clinical records and immunologic studies. The paraffin blocks were sectioned at 5 micron thickness and sections were stained with hematoxylin-eosin and if needed be, methyl-green pyronin. Immunologic studies were also performed on the sections of paraffin-embedded tissue and on 9 cases of frozen sections; immunoperoxidase staining for demonstration of cytoplasmic immunoglobulins and lysozyme and immunofluorescent staining with fluorescein-conjugated monoclonal antibody for demonstration of HLA-DR and Leu-1 antigens in the tumor cells. Prepared elides were examined by light microscope or immunofluorescent miscroscope as occasion demands. The results were as follows: 1) Of the 53 crises of malignant lymphomas, 28 cases(52.9%) and 15 cases(28.2%) were belong to intermediate and high grades respectively. Most of these were "diffuse, small cleaved", "diffuse, mixed", "diffuse, large cell", and "large cell, immunoblastic" types, which ranged from 15.1% to 18.9% respectively. True histiocytic lymphoma was absent. 2) According to Rappaport classification, histiocytic lymphomaa were the most frequent type (30.2%) and the next, lymphocytic, poorly differentiated type (18.9%). 3) Among the 44 crises of morphologically putative B-cell lymphomas, immunoper-oxidase staining for immunoglobulin was positive in 68.2% of crises and immunofluorescent staining for anti-HLA-DR, positive in 65.9%. 4) Monoclonality of light chain was observes in 66.7% of the cases that stained positive for immunoglobulin by immunoperoxidase method. 5) Histiocytes were present and mixed with tumor cells ranging from mild to severe degree in 41.5% of the 44 crises of morphologically putative B-cell lymphomas. In mycosis fungoides histiocytes were mixed with tumor cello in 80%, but in lymphoblastic lymphomas, all cases lacked histiocytes.

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