LPS로 염증 유도된 RAW 264.7세포에 대한 참콩풍뎅이(Popillia flavosellata) 에탄올 추출물의 항염증 효과

윤영일(Young-Il Yoon),황재삼(Jae-Sam Hwang),김미애(Mi-Ae Kim),안미영(Mi Young Ahn),이영보(Young-Bo Lee),한명세(Myung Sae Han),구태원(Tae-Won Goo),윤은영(Eun-Young Yun) 한국생명과학회 2015 생명과학회지 Vol.25 No.9

본 연구에서는 참콩풍뎅이(Popillia flavosellata) 에탄올 추출물(PFE)의 항염증 효능을 분석하기 위해 PFE를 농도별(500, 1,000, 2,000 ㎍/ml)로 대식세포인 RAW 264.7에 처리 시 최고 처리농도인 2,000 ㎍/ml까지 통계적인 유의성 있는 독성이 없음을 확인하였다. LPS (100 ng/ml)로 염증 유도된 RAW 264.7 세포에 PFE를 농도별(500, 1,000, 2,000 ㎍/ml)로 동시 처리 시 농도 의존적으로 염증성사이토카인인 TNF-α와 IL-6의 단백질 생성을 통계적인 유의성(p<0.001)있게 억제함을 확인하였다. 또한 염증 유도된 RAW 264.7 세포에 PFE 동시 처리 시 NF-κB p65의 핵으로 이동이 차단됨과 iNOS와 COX-2의 단백질 발현을 감소시키는 것을 확인하였다. 이상의 연구결과를 통해 참콩풍뎅이는 염증에 의해 활성화된 TLR-4 신호전달과정을 조절하는 NF-κB p65의 활성과 염증성사이토카인 TNF-α와 IL-6의 생성 및 염증성효소 iNOS와 COX-2의 생성을 억제하는 항염증 효능이 있음을 확인하였다. The beetle Popillia flavosellata has been no reported its functional effects. In this study, we investigated the anti-inflammatory effect of P. flavosellata ethanol extract (PFE) on RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for the induction of inflammation. First, we examined the cytotoxicity of PFE in the RAW 264.7 cells at a concentration of 2,000 μg/ml or less. To evaluate the anti-inflammatory effects of PFE, we investigated the expression levels of proinflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and proinflammatory enzymes, such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether PFE inhibited the translocation of nuclear factor kappa B (NF-κB) p65 into the nucleus in the LPS-induced RAW 264.7 cells. We found that the protein levels of TNF-α and IL-6 were decreased in the LPS-induced RAW 264.7 cells after the treatment with PFE in a dose-dependent manner. In addition, we confirmed that PFE inhibited the translocation of NF-κB p65 into the nucleus, as well as the protein expression levels of iNOS and COX-2. Accordingly, we propose that PFE exerts an anti-inflammatory effect through the down-regulation of NF-κB p65, TNF-α, IL-6, iNOS, and COX-2 via the toll like receptor (TLR)-4 inflammatory signaling pathway.

LPS로 유도된 RAW 264.7세포에 대한 벼메뚜기(Oxya chinensis sinuosa) 에탄올 추출물의 항염증 효과

윤영일(Young-Il Yoon),정미연(Mi Yeon Chung),황재삼(Jae-Sam Hwang),구태원(Tae-Won Goo),안미영(Mi-Young Ahn),이영보(Young-Bo Lee),한명세(Myung-Sea Han),윤은영(Eun-Young Yun) 한국생명과학회 2014 생명과학회지 Vol.24 No.4

본 연구에서는 벼메뚜기 에탄올 추출물의 항염증 효능을 분석하기 위해 LPS로 염증 유도된 RAW 264.7 세포를 이용하였다. OCE의 항염증 효능을 확인 하기 위해서, 염증 유도된 RAW 264.7 세포에 대해 OCE 농도 의존적으로 염증성 사이토카인인 TNF-α와 IL-6의 유전자발현 및 단백질 생성을 감소시킴을 real-time PCR과 ELISA로 확인하였다. 또한, NF-κB p65의 핵으로 이동이 차단됨을 면역형광염색으로 확인하였으며, iNOS와 COX-2 단백질 발현을 감소시키는 것을 Western blot 분석으로 확인하였다. 이상의 연구결과를 통해 벼메뚜기는 염증에 의한 NF-κB p65의 활성과 TNF-α와 IL-6의 생성과 iNOS 및 COX-2의 발현을 억제하는 항염증 효능을 갖고 있는 것을 확인하였다. Although the grasshopper Oxya chinensis sinuosa has long been used as food in Korea, there is little data on its functional effects. In this study, we investigated the anti-inflammatory effect of O. c. sinuosa ethanol extract (OCE) in RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for induction of inflammation. First, we determined that there is no cytotoxicity at 2,000 μg/ml or less of OCE in RAW 264.7 cells. To evaluate the anti-inflammatory effects of OCE, we investigated expression levels of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and pro-inflammatory enzymes such as inducible nitric oxide synthase (iNOS) and cyclo-oxygenase- 2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether OCE could inhibit translocation of NF-κB p65 into the nucleus in LPS induced RAW 264.7 cells. As a result, we found that the mRNA and protein levels of TNF-α and IL-6 decreased in LPS-induced RAW 264.7 cells after treatment with OCE in a dose-dependent manner. In addition, we confirmed a 2,000 ug/ml concentration of OCE inhibited translocation of NF-κB p65 by immunnostaining and Western blot analysis, and a decrease in the protein expression levels of iNOS and COX-2. Accordingly, we suppose that OCE has an anti-inflammatory effect through down-regulation of TNF-α, IL-6, iNOS, and COX-2 related to NF-κB p65 inflammatory signaling pathways.

사례연구를 통한 개발도상국 민자발전사업 리스크 경감방안

윤영일,유호선,여영구,Yoon, Young-Il,Yoo, Ho-seon,Yeo, Yeong-Koo 한국플랜트학회 2013 플랜트 저널 Vol.9 No.1

This study investigates the risks that can occur during the development stage of IPP project in developing countries. In case that ECA and MLA cannot participate due to poor credit rating of the country, the diversification of power purchaser in marginal states can be a great help to reduce both market risk and country risk at the same time. In case of thermal power plants and combined cycle power plants, the effect of performance degradation as time passed will be considered and expected profit of sponsors should be maintained. Recently, developing countries are expanding IPP projects to reduce the financing cost and Korean power companies are positively participating in IPP projects. Accordingly, the loss of Korean companies should be minimized by risk management through the risk mitigation methods of this study. 본 연구에서는 개발도상국 민자발전사업의 개발단계에서 발생할 수 있는 리스크를 사업소재국, 사업주 및 시장으로 분류하여 해당 항목별 주요 리스크를 조사하고, 근래 한전 및 발전회사가 수행한 대표적인 3개 사업에 적용하여 구체적인 경감방안을 도출하고자 하였다. 개발도상국에서 민자발전사업 추진 시 전력구매처로서 신용이 부족하여 수출신용기관과 국제개발금융기구의 사업 참여가 어려운 경우, 국가신용이 양호한 주변국으로 전력판매처를 다변화하여 시장 리스크를 경감시키는 동시에 사업소재국 리스크를 경감시켜야 하며 20~30년에 걸쳐 장기간 운영해야 하는 화력발전사업의 경우 운영기간이 경과할수록 열효율도 저하되는 것을 감안하여, 설비운영 경과에 따른 성능저하 영향을 충분히 반영하여 기대수익을 보존하고 우리 기업의 손실을 최소화해야 한다.

인터넷구전커뮤니케이션에 대한 정보원의 신뢰성이 브랜드자산에 미치는 영향

윤영일(Young Il Yoon),하동현(Dong Hyun Ha) 한국호텔외식관광경영학회 2010 호텔경영학연구 Vol.19 No.4

Brand equity and internet WOM communication were subjects on which all hotel enterprises put the highest emphasis and considered establishment of powerful brand equity as their highest priority task. Hotel enterprises utilized internet WOM communication as a means of reinforcing their brand equity, and brand equity was affected in accordance with the source credibility to internet WOM communication. Therefore, the need of research on how the source credibility to internet WOM communication influenced the establishment of brand equity was recognized. Accordingly, this study aimed to closely examine such influence through empirical analysis by categorizing the source credibility into expertise and trustworthiness. 1,000 copies of survey questionnaire produced through preliminary investigations and mock survey were distributed to customers of five-star hotels located in Seoul, Pusan, Kyongju and Jeju over 25-day period from February 12 to March 8. Among the 962 questionnaires completed by the customers who used aforementioned hotels through internet WOM, 420 valid questionnaires were subjected to final analysis. Frequency analysis, factor analysis, reliability analysis, validity analysis and structural equation model analysis, etc. were carried out by using SPSS 11.5 and AMOS 4.0.

호텔기업과 상사의 윤리적 행동이 종사원의 직무만족에 미치는 영항

윤영일(Yoon, Young-Il),오수경(Oh, Soo-Kyung),하동현(Ha, Dong-Hyun) 한국관광레저학회 2011 관광레저연구 Vol.23 No.5

편마비 환자의 재활을 위한 자전거 에르고미터 개발에 관한 연구

윤영일(Young-Il Yoon),권대규(Tae-Kyu Kwon),김정자(Jung-Ja Kim),김동욱(Dong-Wook Kim),김남균(Nam-Gyun Kim) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5

A new cycle ergometer using a Magneto-Rheological (MR) rotary brake system has been developed for rehabilitation of hemiplegia patients to reduce uneven pedaling characteristics. For this purpose, a control method to adjust the resistance of the MR rotary brake in real time based on the magnitude of the muscular force exerted by the subject has been devised so that the mechanical resistance to the pedaling can be minimized when the affected leg was engaged for pedaling. The new bicycle system using MR brake with the real time control of mechanical resistance was found to be effective in recovering the normal pedaling pattern by reducing unbalanced pedaling characteristics caused by disparity of muscular strength between affected and unaffected leg.

조직의 환경특성이 컨벤션기획사의 개인 창의성에 미치는 영향

윤영일(Yoon Young-Il),오수경(Oh Soo-Kyung),하동현(Ha Dong-Hyun) 한국관광레저학회 2012 관광레저연구 Vol.24 No.5

인공위성 탑재컴퓨터 가상머신을 위한 토큰 컴파일러

윤영일(Young_il Yoon),정현아(Hyuna Jung),조현우(Hyunwoo Joe),김형신(Hyungshin Kim) 한국정보과학회 2012 한국정보과학회 학술발표논문집 Vol.39 No.1A

본 논문에서는 인공위성 탑재 컴퓨터의 가상머신에서 사용되는 C 언어와 유사한 언어를 번역하는 SpaceApp 토큰 컴파일러를 개발하였다. SpaceApp은 위성 제어를 위한 지상으로부터 업로드 가능한 프로그램으로, 이를 실행하기 위한 환경으로 자바가상머신과 유사한 SpaceApp 가상머신이 개발되었다. 이러한 가상머신에 필요한 컴파일러는 SpaceApp 언어를 번역할 수 있어야 하고, 실행 가능한 파일 형식으로 변환 되어야 한다. 따라서 논문에서 개발한 컴파일러는 C 언어와 유사하게 SpaceApp 언어를 번역 가능하다. 그리고 인공위성 임무에 맞게 라이브러리를 지원하며, 가상머신에서 실행 가능한 파일 형식을 출력할 수 있도록 구현 했다. 또한 통합 개발 환경으로 이클립스를 이용하여 편의성을 제공하도록 구현하였다.

광주,전남지역 알레르기 환자에서 흡입성 항원에 대한 감작률의 종적 변화와 기온과의 관련성 분석

윤병조 ( Byung Jo Yoon ),김상훈 ( Sang Hun Kim ),김동현 ( Dong Hyun Kim ),고영일 ( Young Il Koh ) 대한천식알레르기학회 2011 천식 및 알레르기 Vol.31 No.2

Background: Recent investigations have reported that climate changes may influence the development of allergic diseases. Objective: We aimed to investigate how sensitization rates to common inhalant allergens would change in association with the alteration in ambient temperature with the passage of time. Method: We included 1,575 subjects who were residing in Gwangju and Chonnam areas and visited our hospital due to allergic symptoms between 2003 and 2008. The data on ambient temperature were obtained from a regional meteorological station. Skin prick tests were performed. Subjects were classified into 5 groups according to the study year: the 2003, 2004, 2006, 2007 and 2008 groups. Result: The regional mean temperatures in Gwangju and Chonnam areas gradually increased with the passage of time. In subjects aged <40 years, sensitization rates to tree pollens such as beech (P<0.05) and alder (P<0.05) and grass pollens such as rye grass (P<0.05) and orchard grass (P<0.05) significantly increased. There were positive correlations between sensitization rates to alder (Rs=0.90, P<0.05), beech (Rs=0.90, P<0.05) or rye grass (Rs=0.90, P<0.05) and the corresponding mean monthly temperature. However, the frequency of atopy and the sensitization rates to dust mites, fungi, cockroach and weed pollens did not increase. Conclusion: Sensitization rates to tree and grass pollens have recently increased in Gwangju and Chonnam areas, which might be associated with global climate changes. (Korean J Asthma Allergy Clin Immunol 2011;31:93-104)

우주용 전가상화 하이퍼바이저 프로토타입 설계

윤영일(Young_il Yoon),조현우(Hyunwoo Joe),정현아(Hyuna Jung),김정석(Jungseok Kim),김형신(Hyungshin Kim) 한국정보과학회 2012 한국정보과학회 학술발표논문집 Vol.39 No.2A