인삼의 세포독성분획의 작용기작에 관한 연구 ( 1 ) - 인삼중의 Petroleum Ether 분획이 동물암세포에서 고분자 물질의 합성에 미치는 영향

윤연숙,이세영,김병수,윤택구 ( Yeon Sook Yun,Se Yong Lee,Byung Soo Kim,Taik Koo Yun 생화학분자생물학회 1980 BMB Reports Vol.13 No.4

The petroleum ether fraction from Korean Ginseng roots has been proved to be cytotoxic to murine lymphocytic leukemia L5178Y cells and murine Sarcoma 180 cells. This petroleum ether fraction which was extracted from Korean ginseng roots inhibited the growth of murine leukemia L5178Y cells and murine Sarcoma 180 cells in vitro, And it also inhibited DNA, RNA and protein synthesis in murine ascitic Sarcoma 180 cells in vitro. The cytotoxicity of this fraction and it`s inhibitory effect on biosynthesis of macromolecules were exponential function of its concentration, The cytotoxicity correlated to the inhibitory effect on the biosynthesis of macromolecules, especially to the inhibitory action on protein synthesis, The inhibitory action of this fraction on the protein synthesis was due to the inhibition of polysome formation: at the concentration which causes 90% inhibition of protein synthesis all the polysomes were dissociated into monosomes, Since messenger RNA degradation was not occurred at this concentration, petroleum ether fraction seems to interfere specifically the initiation of polypeptide synthesis.

가족정책과 돌봄 민주주의: 재가요양보호사 경험을 중심으로

윤연숙 한국가족사회복지학회 2017 가족정책포럼 Vol.4 No.-

Effect of Red Ginseng on Natural Killer Cell Activity in Mice with Lung Adenoma Induced by Urethan and Benzo(a) pyrene

윤연숙,조성기,문혜선,김영주,오영란,윤택구,Yun, Yeon-Sook,Jo, Sung-Kee,Moon, Hae-Sun,Kim, Young-Ju,Oh, Yeong-Ran,Yun, Taik-Koo 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.1

홍삼의 항발암작용 기전을 규명하기 위한 목적의 일환으로 홍삼추출물이 urethan 및 benzo(a)pyrene을 투여한 마우스에서 natural killer(N.K) 세포 활성도 및 폐선종 발생에 미치는 영향을 발암물질 처리 후 48주 동안 검색하였다. N.K 세포 활성도는 urethan 및 benzo(a)pyrene의 처리에 의하여 현저히 저하되었다. 이와 같은 N.K 세포 활성도의 저하는 상기 발암물질 투여 후 4주부터 나타나 24주까지 계속되었으며 홍삼투여에 의하여 정 상대조군의 수준으로 되돌아 왔다. 동시에 urethan에 의해 6주시부터 유발되기 시작한 페선종 역시 홍삼투여에 의해 그 발생빈도가 현저히 억제되었다. 반면 benzo(a)pyrene에 의해 유발된 폐선종은 48주시에 나타나기 시작하였는데 이 시기는 N.K 세포 활성도가 대조군에서 조차 너무 낮은 수준으로 떨어져 있어 홍삼의 영향을 받을 수 없었는바 홍삼 투여에 의한 폐선종발생빈도 억제효과는 나타나지 않았다. 결론적으로 저자들은 본 연구를 통하여 홍삼에 의한 항발암 효과는 N.K 세포 활성도의 증대와 관련되어 있음을 알 수 있었다. It was previously reported that red ginseng extract inhibited carcinogenesis by urethan, DMBA and aflatoxin $B_1$, (Cancer Detection and Prevention, 6: 515-525, 1983). In an attempt to investigate the mechanism of the anticarcinogenic effect of ginseng, the N.K activity and the incidence of lung adenoma were followed over a period of 48 weeks post injection with urethan or benzo(a)pyrene. The N.K activity was markedly depressed from 4 weeks to 24 weeks after injection of carcinogens. This decreased N.K activity was returned to the level of controls by administration of ginseng. At the same time, lower incidence of lung adenoma noted following administration of ginseng to urethan-injected mice. However, the lung adenoma induced by benzo(a)pyrene began to occure at 48 weeks in which N.K activity had naturally declined to a level too low to be affected by ginseng, and administration of ginseng did not decrease the incidence. In conclusion, these results suggest that the anticarcinogenic effect of ginseng may be related to the augmentation of N.K activity.

有機監素系 化合物(γ-BHC)汚染 Mouse의 脂肪 代謝에 關한 硏究

尹蓮淑 보건장학회 1976 보건장학회보연구논문집 Vol.5 No.-

서비스업 종사자의 고객지향성에 따른 임파워먼트가 고객지각가치와 고객만족에 미치는 영향

윤연숙,최민철,이영일 한국전문경영인학회 2018 專門經營人硏究 Vol.21 No.2

The study aims to investigate the effect of empowerment based on customer-oriented service personnel on Customer Perceived Value and Customer Satisfaction. For this purpose, a total of six hypothesized research models were established and verified. 172 users were included in the experiment based on scenario planning technique which utilized a 2×2 factorial design with independent variables of service provider 's customer orientation and empowerment. The dependent variables were customer perceived value and customer satisfaction. The result illustrates that the customer perceived value and customer satisfaction are higher when service provider has a high degree of customer orientation. Similarly, customer perceived value and customer satisfaction are higher when empowerment is high. It is noteworthy, however, that in case of high empowerment, the degree of service provider’s customer orientation did not make statistically significant difference in customer perceived value and customer satisfaction. This implies that the empowerment is more important than service provider's customer orientation when customers perceive the quality of the service.

성평등 전략으로써 기본소득(Basic Income)의 함의에 관한 연구

윤연숙 이화여자대학교 한국여성연구원 2012 여성학논집 Vol.29 No.1

본 글의 목적은 성평등 전략으로써 기본소득이 어떠한 함의가 있는지 살펴보는 데있다. 현재 복지국가의 사회보장체계는 고용을 전제로 한 남성생계부양자와 가족을 기본 단위로 한다. 그러나 이러한 전제들이 더 이상 유효하지 않은 상황에서 “모든 사람에게 아무런 조건 없이 매월(또는 그 밖의 일정한 기간마다) 소득을 지급하는 제도”로써 기본소득은 현재 복지국가가 처한 위기 상황을 극복할 수 있는 구체적인 전략으로 대두된다. 기존의 사회보장체계와 달리 가족단위가 아닌 개인을 대상으로 하고, 자산과 소득의 유무를 조사하지 않고 일정액의 소득이 주어지며, 근로 의무가 부과되지 않는다는 점에서 기존의 사회보장체계와 차이가 있다. 이처럼 복지국가 위기를 타개할 수 있는 새로운 전략으로써 기본소득이 대두됨에 따라 젠더 관점에서 기본소득이 어떠한 함의가 있는지에 대한 여성주의자들의 논의가 이루어지고 있다. 기본소득이 도입된다면 성별분업이 완화되고 성평등이 증진될 수 있다는 점에서 제도 도입을 찬성하는 입장과 함께, 기존 젠더 규범에 대한 변화 없이 기본소득이 도입되었을 때 성별분업을 고착화할 뿐만 아니라 이를 강화될 수 있다는 점에서 비판이 제기되기도 한다. 본 글에서는 ‘기본소득’(basic income)과 ‘사회적 지분’(stakeholding)으로 대표되는 기본소득의 성격과 유형을 살펴보고, 여성주의 내에서의 기본소득을 둘러싼 논쟁 지점을 통해 젠더관점에서 기본소득이 어떠한 함의가 있는지 살펴보고자 한다. The purpose of this study is to analyze implication of a basic income from gender point of view. Traditional social security systems of welfare state are premised in a male breadwinner and his family. As we experienced the crisis of world economy in 1980's, this premise of welfare state was not valid. Social security system needs modifications respond to changing. In this situation, basic income can be a possible strategy to overcome this crisis. Different from existing social security systems, a basic income is an income paid by a political community to all its members on an individual basis, without means test or work requirement. The implementation of a basic income will have different effects for different groups of women because a basic income is controversial topic with reference to gender equality. Some of them are clearly positive, some of them are negative. Those who favour basic income insist that it can protect women against the consequences of the gendered division of labor, and improve women’s autonomy. On the other hand, feminists are opposed to basic income worry about basic income can make privatization of care work and fix gender norms. Therefore, I will examine basic income’s types and characters first, and seek possibilities of basic income that can accomplish gender equality.

만성 C 형간염바이러스 감염에서 Core 단백과 NS3 단백에 대한 말초혈액단핵세포의 면역반응

윤연숙,이기호,최요한,정숙향,양민진 대한간학회 2001 Clinical and Molecular Hepatology(대한간학회지) Vol.7 No.3

Badqground/Aims' The aims of our study are to assess the frequency of peripheral blood mononuclear cell (PBMC) proliferation and cytokine profiles to hepatitis C virus (HCV) core protein and NS3 protein to search the potential immunosuppressive effect of HCV core in chronically HCV-infected patients. Subjects and Methods' Thirty two anti-HCV-positive patients with chronic liver diseases, eight HBsAg-positive patients with chronic liver diseases, and six healthy adults were the subjects of our study. Using recombinant HCV core and NS3, proliferative response of PBMC and cytokine production were determined. Results' Fifty nine percent and thirteen percent of patients with HCV-related chronic liver diseases showed positive PBMC proliferation to HCV core and NS3, respectively. Thirty four percent and fifty nine percent of patients with HCV-related chronic liver diseases showed significant production of interferon-gamma to HCV core and NS3, respectively. IL-4 production was negligible. When the PBMC were treated with HCV core and NS3 concurrently, or HCV core and phytohemagglutinin conaxvently, the stimulation indices were significantly decreased compared to those treated either with NS3 or PHA without core. Conclusions' Although about two thirds of chronically HCV-infected patients with liver diseases showed the PBMC proliferation and Th 1 type cytokine profile, they could not eradicate the viral infection. This ineffective immune response seems to play a role in the pathogenesis of chronic inflammatory liver disease resulting in liver cirrhosis and hepatocellular carcinoma. HCV core showed a potential immunosuppressive effect, which has important meaning for the mechanism of HCV persistence. (Korean J Hepatol 2001;7:292-298)

홍삼이 Urethan 및 Benzo ( a ) pyrene 에 의하여 폐선종이 유발된 마우스에서 Natural killer 세포 활성도에 미치는 영향

윤연숙,조성기,문혜선,김영주,오영란,윤택구 ( Yeon Sook Yun,Sung Kee Jo,Hae Sun Moon,Young Ju Kim,Yeong Ran Oh,Taik Koo Yun ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.1

It was previously reported that red ginseng extract inhibited carcinogenesis by urethan, DMBA and aflatoxin B₁, (Cancer Detection and Prevention, 6: 515-525, 1983). In an attempt to investigate the mechanism of the anticarcinogenic effect of ginseng, the N.K activity and the incidence of lung adenoma were followed over a period of 48 weeks post injection with urethan or benzo(a)pyrene. The N.K activity was markedly depressed from 4 weeks to 24 weeks after injection of carcinogens. This decreased N.K activity was returned to the level of controls by administration of ginseng. At the same time, lower incidence of lung adenoma noted following administration of ginseng to urethan-injected mice. However, the lung adenoma induced by benzo(a)pyrene began to occure at 48 weeks in which N.K activity had naturally declined to a level too low to be affected by ginseng, and administration of ginseng did not decrease the incidence. In conclusion, these results suggest that the anticarcinogenic effect of ginseng may be related to the augmentation of N.K activity.

인삼의 세포독성분획의 작용기작에 관한 연구 ( 2 ) - 인삼중의 Ethyl acetate 분획이 동물암세포에서 고분자 물질의 합성에 미치는 영향

윤연숙,이세영,기병수,윤택구 ( Yeon Sook Yun,Se Yong Lee,Byung Soo Kim,Taik Koo Yun ) 생화학분자생물학회 1980 BMB Reports Vol.13 No.4

The ethyl acetate fraction from Korean Ginseng roots has been shown to be cytotoxic to murine lymphocyitic leukemia L5178Y cells and murine Sarcoma 180 cells. This ethyl acetate fraction which was extracted from Korean Ginseng root inhibited the growth of murine leukemia L5178Y cells and murine Sarcoma 180 cells in vitro by an exponential function of its concentration, DNA, RNA and protein synthesis in murine ascitic Sarcoma 180 cells were also inhibited in vitro in a similar fashion, The cytotoxicity of this fraction correlates to the inhibitory effect on the biosynthesis of marcomolecules. The synthesis of DNA and RNA were much more sensitive to this fraction than that of potein, indicating that the inhibitory action on the biosynthesis of nucleic acids is responsible for the inhibition of protein synthesis, However, the cytotoxic activity of this fraction does not seem to be due to the inhibitory activity on the DNA synthesis or to the gross inhibition of RNA synthesis since the synthesis of DNA and RNA were inhibited only 40-45% at the concentration where 70% cell death occurred, In order to examine the possibility that the cytotoxicity of the ethyl acetate fraction is due to the inhibitory activity on the particular RNA species, we compared the inhibitory activity of this fraction on various RNA species with that of actinomycin D. At lower concentrations, this fraction inhibited the synthesis of the heterogeneous nuclear RNA much more than any other RNA species. The synthesis of ribosomal RNA was also sensitive, while the messenger RNA and transfer RNA synthesis were relatively insensitive to this fraction. At high concentration, the fraction inhibited the synthesis of all species of RNA.

유산균과 건강

尹連淑 이화여자대학교 대학원 1977 硏究論叢 Vol.7 No.-