정신건강의학과 환자의 절망감과 자살사고의 관계: 수면의 질과 모호함에 대한 해석 편향의 매개효과

윤소미,김은경,김대호,박용천 한국정신신체의학회 2023 정신신체의학 Vol.31 No.2

연구목적본 연구는 정신건강의학과 환자의 절망감이 자살사고에 미치는 영향에서 수면의 질과 모호함에 대한 해석편향의 매개효과를 확인하고자 하였다. 방 법한양대학교 구리병원 정신건강의학과의 외래 환자와 입원 환자를 대상으로 축적된 자료를 사용하였으며, 측정 도구로는 벡 절망 척도(Beck Hopelessness Scale), 피츠버그 수면의 질 척도(Pittsburgh Sleep Quality Index), 모호한 상황에서의 시나리오 척도(Ambiguous/Unambiguous Situations Diary-Extended Version, AUSD-EX), Ultra-Short Suicidal Ideation Scale이 사용되었다. 231명의 자료를 기초로 SPSS 25.0과 SPSS Macro를 사용하여 상관분석과 Bootstrapping 기법을 이용한 회귀분석을 실시하였다. 결 과연구 결과, 절망감과 자살사고의 관계에서 수면의 질과 모호함에 대한 해석 편향의 이중 매개효과가 유의하게 나타났다. 결 론본 연구는 절망감이 낮은 수면의 질과 모호함에 대한 해석 편향을 거쳐 자살사고에 순차적으로 영향을 미친다는 점을 확인하였다. 이러한 결과는 정신건강의학과 환자에서 절망감이 자살사고로 이어지는 것을 예방함에 있어 낮은 수면의 질과 모호함에 대한 해석 편향을 개선하는 것이 필요함을 시사한다. Objectives:This study aimed to examine the mediating effect of sleep quality and interpretation bias for ambiguity in the relationship between hopelessness and suicidal ideation in psychiatric patients. Methods:A total of 231 psychiatric outpatients and inpatients completed the Beck Hopelessness Scale, Pittsburgh Sleep Quality Index, Ambiguous/Unambiguous Situations Diary-Extended Version, and Ultra-Short Suicidal Ideation Scale. Data analysis was conducted using regression analyses and bootstrap sampling. Results:The results of this study showed that hopelessness had a direct effect on suicidal ideation, and that sleep quality and interpretation bias for ambiguity mediated the association between hopelessness and suicidal ideation. Moreover, there was a significant double mediating effect of sleep quality and interpretation bias for ambiguity on the relationship between hopelessness and suicidal ideation. Conclusions:These results suggest that it is important to consider both sleep quality and interpretation bias for ambiguity to prevent hopelessness from leading to suicidal idea. These results suggest that considering both sleep quality and interpretation bias for ambiguity may be important in preventing hopelessness from leading to suicidal ideation.

Cartilage regeneration using a biocompatible hydrogel in vivo

윤소미,김다연,이빛나,이봉,민병현,김재호,김문석 한국고분자학회 2012 한국고분자학회 학술대회 연구논문 초록집 Vol.2012 No.4

In vivo sustained release of protein drugs using small intestinal submucosa

윤소미,강꽃님,김다연,이봉,민병현,김재호,김문석 한국고분자학회 2011 한국고분자학회 학술대회 연구논문 초록집 Vol.2011 No.2

PP2A negatively regulates the hypertrophic response by dephosphorylating HDAC2 S394 in the heart

윤소미,국태원,민현기,권덕화,조영국,김미라,신세라,정호석,정승훈,이수민,강가은,박윤철,김용숙,안영근,Julie R. McMullen,Ulrich Gergs,Joachim Neumann,김경근,김정철,남광일,김영국,국현,엄광현 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Cardiac hypertrophy occurs in response to increased hemodynamic demand and can progress to heart failure. Identifying the key regulators of this process is clinically important. Though it is thought that the phosphorylation of histone deacetylase (HDAC) 2 plays a crucial role in the development of pathological cardiac hypertrophy, the detailed mechanism by which this occurs remains unclear. Here, we performed immunoprecipitation and peptide pull-down assays to characterize the functional complex of HDAC2. Protein phosphatase (PP) 2 A was confirmed as a binding partner of HDAC2. PPP2CA, the catalytic subunit of PP2A, bound to HDAC2 and prevented its phosphorylation. Transient overexpression of PPP2CA specifically regulated both the phosphorylation of HDAC2 S394 and hypertrophyassociated HDAC2 activation. HDAC2 S394 phosphorylation was increased in a dose-dependent manner by PP2A inhibitors. Hypertrophic stresses, such as phenylephrine in vitro or pressure overload in vivo, caused PPP2CA to dissociate from HDAC2. Forced expression of PPP2CA negatively regulated the hypertrophic response, but PP2A inhibitors provoked hypertrophy. Adenoviral delivery of a phosphomimic HDAC2 mutant, adenovirus HDAC2 S394E, successfully blocked the anti-hypertrophic effect of adenovirus-PPP2CA, implicating HDAC2 S394 phosphorylation as a critical event for the anti-hypertrophic response. PPP2CA transgenic mice were protected against isoproterenolinduced cardiac hypertrophy and subsequent cardiac fibrosis, whereas simultaneous expression of HDAC2 S394E in the heart did induce hypertrophy. Taken together, our results suggest that PP2A is a critical regulator of HDAC2 activity and pathological cardiac hypertrophy and is a promising target for future therapeutic interventions.

운동과 마이크로바이옴: Post Covid-19의 새로운 웰니스 기대

윤소미 한국웰니스학회 2020 한국웰니스학회 학술발표회 Vol.2020 No.11

설사성 패류독의 LC-MS/MS에 의한 분석

윤소미,장준호,신일식,이종옥,이종수,Yun, So-Mi,Jang, Jun-Ho,Shin, Il-Shik,Lee, Jong-Ok,Lee, Jong-Soo 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.7

LC-MS/MS법에 의하여 설사성 패류독소 7종(okadaic acid, dinophysistoxin-1, -3, pectebotoxin-1, -2, -6, yessotoxin)의 동시 분석 조건을 확립하였고, 2006년 3월부터 9월까지 매월 5종의 이매패(굴, 진주담치, 반지락, 가리비, 개조개, 6월부터 9월까지는 가리비 대신 피조개)를 정기적으로 통영시장에서 구입하여 분석하였으며, 기존의 마우스 검사법과 비교하였다. 마우스 검사법으로는 총 35종 시료 중 3월의 굴과 진주담치에서 0.05${\sim}$0.1 MU/g의 독성을 나타내었으나, 이미 알려진 설사성 패류독소는 전혀 검출되지 않아 다른 성분에 의한 것으로 판단되었다. 한편, LC-MS/MS법에 의하여서는 6월 진주담치에서 dinophysistoxin-1(DTX1)이 0.05 ${\mu}g/g$ 검출되었으며, 8월의 모든 시료에서 okadaic acid(OA)가 0.01${\sim}$0.02 ${\mu}g/g$ 검출되었다. 이들은 외국의 기준치[0.05 MU/g(일본), OAs(OA+DTX1) 0.16 ${\mu}g/g$(EU)]보다 매우 낮은 함량으로 식품 위생상 안전하였다. Diarrhetic shellfish poisons (DSP) such as okadaic acid (OA), dinophysistoxin-1 (DTX1), pectenotoxin-1(PTX1), PTX2, PTX6 and yessotoxin (YTX) were determined simultaneously by LC-MS/MS and mouse bioassay in the shellfishes (oyster, mussel, Washington purple clam, ark shell, scallop and short necked clam) collected at Tongyeong, from March to September, 2006. Oyster and mussel were found to contain DSP (0.05${\sim}$0.1 MU/g) in March by mouse bioassay; however, no DSP components were detected on the LC-MS/MS. Also, a small amount of DTX1 (0.05 ${\mu}g/g$) in mussel (June) and OA (0.01${\sim}$0.02 ${\mu}g/g$) in 5 species of shellfishes(August) were determined by LC-MS/MS.

임신과 출산 후 걷기 강도에 따른 운동 및 회복 반응

윤소미,손윤선,황은진,이윤빈,황봉연,이대택 한국운동생리학회 2017 운동과학 Vol.26 No.4

PURPOSE: To examine the influence of the walking exercise intensity during pregnancy and postpartum on physiological and perceived exertion responses. METHODS: Nine participants walked on a treadmill at 3, 5, and 6 km/h for 10 minutes followed by resting for 10 minutes after each speed. During exercise and resting, heart rate (HR), oxygen uptake (VO2), and perceived exertion (RPE) were measured. RESULTS: During pregnancy, HR at 5 and 6 km/h walking and the recovery HR from walking was higher at 3, 5 and 6 km/h than postpartum (p<.05). VO2 during pregnancy was higher than postpartum at 3 and 5 km/h walking (p<.05, p<.01). During pregnancy, RPE at 5 and 6 km/h walking was higher than postpartum (p<.001). Three pregnant women, due to abdominal cramp during walking at 6 km/h, no statistical comparisons in VO2 and RPE were adequate. During pregnancy, HR was higher as the exercise intensity increased, and the recovery was delayed. VO2 of pregnant women was higher during exercise than postpartum. CONCLUSIONS: Since pregnancy changes many aspects of physiological and physical status, long term and systemic longitudinal studies providing objective index are warranted.

Chimeric Antigen Receptor T Cell Therapy: A Novel Modality for Immune Modulation

윤소미,엄광현 전남대학교 의과학연구소 2020 전남의대학술지 Vol.56 No.1

Cancer remains a leading cause of death, despite multimodal treatment approaches. Even in patients with a healthy immune response, cancer cells can escape the immune system during tumorigenesis. Cancer cells incapacitate the normal cell-mediated immune system by expressing immune modulation ligands such as programmed death (PD) ligand 1, the B7 molecule, or secreting activators of immune modulators. Chimeric antigen receptor (CAR) T cells were originally designed to target cancer cells. Engineered approaches allow CAR T cells, which possess a simplified yet specific receptor, to be easily activated in limited situations. CAR T cell treatment is a derivative of the antigen-antibody reaction and can be applied to various diseases. In this review, the current successes of CAR T cells in cancer treatment and the therapeutic potential of CAR T cells are discussed.

중등학교 운동선수들의 종목별 특성 분류에 따른 하지 근력 분석

윤소미,임기훈,박철형 제주대학교 교육과학연구소 2020 교육과학연구 Vol.22 No.1

The purpose of this paper is to strength of lower extremity by the event characteristics of secondary school athletes. The subjects were 123 male elite athletes (7 sports) from Jeju middle school (7 schools, 65 students) and high school (11 schools, 58 students), and measured the subject's height, weight and Maximal Voluntary Contraction (MVC) and Rate of Force Development (RFD). As a result, first, there was a positive correlation between MVC and RFD. Second, there was a statistically significant difference in the strength levels of similar event sports at the MVC. Third, the anaerobic event showed statistically significant higher MVC than the aerobic event. Fourth, the higher the MVC than the average level, the more significant effect on the anaerobic event. At present, athletic performance is the most important indicator for predicting a athletes's performance. Understanding the characteristics of each event and applying appropriate training programs are essential elements for systematically nurturing student athletes and maximizing future development. 본 연구는 증등학교 운동선수들의 종목별 특성에 따른 하지 근력을 분석하는데 목적이 있다. 제주지역 중학교(7개교, 65명) 및 고등학교(11개교, 58명) 남자 엘리트 운동선수(7개 종목) 123명을 연구대상자로 선정하였으며, 신장, 체중, 하지 근력(자발적 최대근력(Maximal Voluntary Contraction, MVC) 및 힘 생성 비율(Rate of Force Development, RFD))을 측정하였다. 연구 결과 첫째, MVC와 RFD에서 정적 상관관계가 나타났다. 둘째, 유사종목별 근력 수준은 MVC에서 통계적인 유의한 차이가 나타났다. 셋째, 무산소성 종목이 유산소성 종목에 비해 MVC가 통계적으로 유의하게 높은 것으로 나타났다. 넷째, MVC가 평균 수준보다 높을수록 무산소성 종목에 유의한 영향을 미치는 것으로 나타났다. 운동능력을 통해 선수의 경기력을 예측하고, 종목별 특성을 이해하여 적합한 훈련프로그램을 적용한다면, 학생선수들을 체계적으로 육성하고 향후 발전 가능성을 극대화하는데 도움될 수 있을 것이라 기대된다.

미역 추출물로부터 충치 원인균, Streptococcus mutans에 대한 항균물질의 분리 및 동정

윤소미,장준호,이종수,Yun, So-Mi,Jang, Jun-Ho,Lee, Jong-Soo 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.2

건조 미역 10.4 kg을 acetone으로 추출, 여과한 다음 액-액 분배, 각종 크로마토그라피를 통하여 충치원인균, Streptococcus mutans에 대한 항균물질의 한 성분을 분리, 정제하고(160 mg, 수율 $1.5\times10^{-3}$%) 물질을 동정한 결과는 다음과 같다. 건조 미역 30 g 상당량의 추출액을 기준으로 용매 획분별 항균활성을 조사하였을 때, $CHCl_3$층이 73.2%로 가장 강하였고, hexane층이 62.0%이었으며, BuOH층이나 물층은 거의 없었다. 알루미나 칼럼에서는 산성 획분인 1% $NH_4OH:MeOH(1:1)$용매 획분이 81.0%의 항균활성을 나타내었으며, 실리카 칼럼에서는 $CHCl_3:MeOH(95:5)$용매에서 가장 높은 95.5%의 항균성을 나타내었고, ODS칼럼에서는 85% MeOH에서 96.4%의 항균성을 나타내었다. 최종적으로 ODS칼럼에서 95% MeOH를 이동상으로 하여 3개의 물질 S1(10 mg), S2(90 mg), S3(60 mg)을 분리 정제하였다. TLC에서 각 성분은 동일한 Rf값 0.42를 나타내어 동일한 물질로 추정되었으며, 이들을 메칠 유도체화한 성분들은 Rf값이 0.95로 바뀌어 이들 물질이 carboxyl기를 가지는 지방산으로 추정되었다. GC분석에서 표품 지방산과 비교한 결과, 이들은 $C_{18:4,n-3}$ 지방산과 retention time이 일치하였다. 또한, 메칠 유도체의 mass spectrum 분석 결과, m/z 290에 분자 이온 peak가 관측되어 $C_{18:4,n-3}$ 지방산의 methyl 유도체의 분자량과 일치하여, 이 물질을 3,6,9,12-octadecatetraenoic acid(stearidonic acid, $C_{18:4,n-3}$) 지방산으로 동정하였다. An antibacterial substance to the Streptococcus mutans, a causative bacterium for decayed teeth, was isolated from the dried sea mustard, Undaria pinnatifida, and identified by GC and GC/MS. Acetone extract from the sea mustard (10.4 kg), was evaporated and partitioned to 4 fractions such as hexane, chloroform, butanol and water. The most active chloroform fraction were further purified through basic alumina, silicic acid and ODS column, successively, and finally, 3 antibacterial substances were isolated on the HPLC attached ODS column by using 95% MeOH and guided with UV detector (254 nm). Antibacterial substances (total 160mg, yield $1.5\times10^{-3}$%) had the same Rf value (0.42) on the TLC developed hexane diethyl ether acetic acid (80:30:1) and those methyl esters moved to 0.95. They were identified as the same unsaturated fatty acid, $C_{18:4,\;n-3}$ (3,6,9,12-octadecatetraenoic acid, stearidonic acid) compared relative retention times (15.5 min) with authentic fatty acid on the GC chromatogram. It was further confirmed unambiguously on the GC/MS giving molecular ion peak at m/z 290 which coincided with its methyl ester.