足三里의 竹瀝 藥鍼이 Intraluminal Filament 揷入術에 의하여 誘發된 白鼠의 虛血性 局所 腦損傷에 미치는 影響

윤대환,이봄비,심인섭,나창수 경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.2

Objectives : Bambusae Caulis in Liquamen(BCL) has been used to medication for early stroke in the Oriental Medicine. So this study was planned to investigate the effects of BCL on the focal ischemia-induced by intraluminal filament insertion in the rats. Materials and methods : The focal ischemia was induced by Intraluminal Filament insertion into middle cerebral artery. BCL herbal acupuncture at ST36 was carried out during 3 weeks after focal ischemia-induced. Eight-arm radial maze was used for the behavioral task and neuroprotective effect of BCL acupuncture was observed by Cresyl violet, AchE, ChAT-stainResults : The error rate in the eight-arm radial maze task was significantly decreased in BCL1(0.89mg/kg) herbal acupuncture group compared to control group on 3,4,5days, compared to saline acupuncture group on 3,5days. The rate of correct choice was significantly increased in saline acupuncture, BCL1 herbal acupuncture group, BCL2(0.089mg/kg) herbal acupuncture groups compared to control group. The density of neurons in the hippocampal CA1 was the most increased in BCL1 herbal acupuncture group compared to the others. The density of AchE in the hippocampal CA1 was significantly increased in BCL1 herbal acupuncture, BCL2 herbal acupuncture groups compared to control group, and in BCL2 herbal acupuncture groups increased compared to saline acupuncture, control groups. The density of ChAT in the hippocampal CA1 was significantly increased in BCL1 herbal acupuncture group compared to saline acupuncture, control groups. Conclusions : These results suggest that BCL herbal acupuncture could be used as a medication for controlling the early stroke

대돈(大敦)${\cdot}$용천(湧泉) 염전(捻轉) 및 영수(迎隨) 보사수기법(補瀉手技法)이 2K1C에 의하여 유발(誘發)된 고혈압(高血壓) 백서(白鼠)에 미치는 영향(影響)

윤대환,한정희,김선희,나창수,Youn, Dae-Hwan,Han, Jeong-Hee,Kim, Suhn-Hee,Na, Chang-Su 경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.3

Objectives : The purpose of this study was to examine effects of acupuncture by needle manipulation at $LR1{\cdot}Kl1$ on the blood pressure, plasma levels of ANP(atrial natriuretic peptide), renin and cardiomegalic index in hypertensive rat Induced by two kidney one clip(2K1C). Material and methods : The groups divided into 7 groups; Control, no treatment. Acu-1,acupuncture at $LR1{\cdot}Kl1$ bilaterally and the needle was twirled and rotated forward with the thumb of the right hand 6 times. Acu-2, acupuncture at $LR1{\cdot}Kl1$ bilaterally and the needle was twirled and rotated forward with the forefinger of the right hand 6 times. Acu-3, acupuncture at $LR1{\cdot}Kl1$ bilaterally and the needle was inserted in the opposite direction(body direction) as the channel runs, Acu-4, acupuncture at $LR1{\cdot}Kl1$ bilaterally, the needle was inserted in the opposite direction(body direction) as the channel runs and the needle was twirled and rotated forward with the forefinger of the right hand 6 times. The acupuncture executed 5times for 2 weeks. Results : Systolic blood pressure was decreased significantly in Acu-2, Acu-3 and Acu-4. Plasma renin was decreased significantly in Acu-2 and Acu-4. Plasma ANP was increased significantly in Acu-2, however was decreased significantly in Acu-4. Cardiac hypertrophy index was decreased significantly in Acu-1, Acu-2, Acu-3 and Acu-4. Conclusions : These results suggest that acupuncture by needle manipulation at $LR1{\cdot}Kl1$ is effective in hypertension by renal blood problem.

직자(直刺), 영수(迎隨) 및 염전수기법(捻轉手技法)에 따라 시행한 경거(經渠)${\cdot}$복류(復溜) 침자(鍼刺)가 중대뇌동맥(中大腦動脈) 폐새(閉塞)에 의하여 유발(誘發)된 국소(局所) 뇌허혈(腦虛血) 백서(白鼠) hippocampus의 항세포자멸사(抗細胞自滅死) 및 복경보호(福

윤대환,변정윤,최찬헌,백진웅,정지연,정연진,나창수,Youn, Dae-Hwan,Byun, Jeng-Yun,Choi, Chan-Hun,Baek, Jin-Ung,Jeong, Ji-Yeon,Jung, Yeon-Jin,Na, Chang-Su 경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.3

Objectives : Aims of this study is to investigate the effects of $LU8{\cdot}KI7$ in rat induced by experimental focal ischemia. Materials and methods : The focal ischemia was induced by intraluminal filament insertion into middle cerebral artery. The groups divided into 6groups, control; no therapy group after ischemia-induced, AT1; acupuncture therapy group at $LU8{\cdot}KI7$ after ischemia-induced, AT2; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally and the needle was twirled and rotated forward with the thumb of the right hand 9times, AT3; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally and the needle was twirled and rotated forward with the forefinger of the right hand 9times, AT4; acupuncture therapy at$LU8{\cdot}KI7$ bilaterally and the needle was inserted to the direction following the flowing route of the meridian(digital direction), AT5; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally, the needle was inserted to the direction following the flowing route of the meridian(digital direction) and the needle was twirled and rotated forward with the thumb of the right hand 9times. Acupuncture therapy was carried out 7times during 2weeks after focal ischemia-induced. The anti-apoptotic and neuroprotective effects of acupuncture are observed by Bax, Bcl-2, mGluR5, cytochrome c, Cresyl violet and ChAT-stain. Results : The intensity of Bax was decreased in AC1, AC4, AC5 group, was increased in AC2, AC3 group. The intensity of Bcl-2 was increased in AC2, AC3, AC4, AC5 group. The intensity of mGluR5 was decreased in AC1 group, was increased in AC4, AC5 group. The intensity of Cytochrome c was increased in ACI, AC2 group, was decreased in AC4, AC5 group. The density of neurons stained by Cresyl violet was increased in all group without control group. The density of ChAT was increased in AC2, ACS group. Conclusions : Our study suggests that AC5 group show anti-apoptotic and neuroprotective effects on cholinergic neuron in focal cerebral ischemia of the stroke in rats.

족삼리(足三里) 침자(鍼刺)가 인체(人體)의 Skin temperature, Galvanic skin response 및 Heart rate에 미치는 영향(影響)

윤대환,나창수,Youn Dae-Hwan,Na Chang-Su 경락경혈학회 2003 대한침구의학회지 Vol.20 No.3

Objectives : The purpose of this study was to investigate the Effect of Acupuncture Chok-samni(ST36) on Skin temperature(ST), Galvanic skin response(GSR) and Heart rate(HR) in humans. ST36 is used in uneasiness, high fever, stroke, paralysis on oriental medicine. Methods : Subjects were 18 healthy oriental medical students volunteers(15male, 3female, mean age-28). Under sequence of ST36 acupuncture, the first group was observed ST, the second group was observed GSR and the third group was observed HR. Skin temperature was measured on Haegye acupoint(ST41) before and after acupuncture stimulation. Each test took 15minutes. This test was performed twice in order to increase the statistical authenticity. Results : Our result indicates that acupuncture stimulation of ST36 statistically decreased skin temperature on ST41. Also statistically decreased in HR. The change of GSR after ST36 acupuncture was decreased, but there is no statistical. Conclusions : These results suggested that acupuncture stimulation of ST 36 has effect on ST and HR in human body, and may be used by treatment as control the nerve system.

直刺, 迎隨 및 捻轉手技法에 따라 시행한 經渠·復溜 鍼刺가 中大腦動脈 閉塞에 의하여 誘發된 局所 腦虛血 白鼠 hippocampus의 抗細胞自滅死 및 神經保護에 미치는 影響

윤대환,최찬헌,백진웅,정연진,나창수,정지연,변정윤 경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.3

전남 함평산 자색양파 추출물의 항균효과와 피부장벽 보호효과

윤대환,신헌태,Youn, Dae-Hwan,Shin, Heon-Tae 대한예방한의학회 2012 대한예방한의학회지 Vol.16 No.1

Objective : In this study, we assessed the anti-bacterial effects and epidermal permeability barrier function of red onion juice comparing to yellow onion juice and $Houttuynia$ $cordata$ extract $in$ $vitro$. Methods : 3types of red and yellow onion juice were prepared as antibacterial agent candidates with Houttuynia cordata hot water extract using 4 different bacterial strains ($Escherichia$ $coil$, $Salmonella$ $enterica$ $subsp.$ $enterica$, $Staphylococcus$ $epidermidis$, $Staphylococcus$ $aureus$ $subsp$) by colony counting method. The expression of filaggrin, a marker of keratinocyte differentiation, and serine palmitoyl transferase (SPT), a marker of the formation of the stratum corneum lipid barrier, in human HaCat keratinocytes were analyzed using HaCaT cell line. The expression of COX-2 and AP-1 which is a factor of COX-2 transcription were also analyzed by western blotting method. Results : There was detectable anti-bacterial effects on $Staphylococcus$ $epidermidis$, $Staphylococcus$ $aureus$ $subsp$ among 1%, 5%, 10% extracts of yellow and red onion.(81%-100%) The bacteriocidal effects were not shown on $Escherichia$ $coil$, $Salmonella$ $enterica$ $subsp.$ $enterica$ among $Houttuynia$ $cordata$, yellow onion and red onion extracts. The in vitro results showed the concentration-dependent effects on the expression of both filaggrin and SPT in HaCat cells among 0.01%, 0.05%, 0.1%, 0.5% extracts in Houttuynia cordata and red onion, reflecting the notion that $Houttuynia$ $cordata$ and red onion can induce epidermal keratinocyte differentiation and improve the recovery of skin barrier functions. The concentration-dependent effects also have been shown on the expression of both COX-2 and AP-1 among 0.01%, 0.05%, 0.1%, 0.5% extracts in $Houttuynia$ $cordata$ and red onion, while slight effect in yellow onion. Conclusion : Red onion juice could be a potential candidate enhanser for the skin care and cosmetology. 1. 추출방법, 추출농도에 따라 항균력의 차이가 있었으나, 일반양파, 자색양파의 1%, 5%, 10% 생즙추출물 모두, 피부상재균인 Staphylococcus epidermidis(KCTC1917), Staphylococcus aureus subsp(ATCC6538)에 양호한 항균력을 보였다(81%~100%). 어성초 열수추출물은 10% 고농도에서만 피부상재균인 Staphylococcus epidermidis(KCTC1917), Staphylococcus aureus subsp(ATCC6538)에 항균력을 보였다(100%). 2. 자색양파, 어성초, 일반양파의 1%, 5%, 10% 추출물 모두, 식중독 균인 Escherichia coil, Salmonella enterica subsp. enterica에서는 항균력을 보이지 않았다. 3. 자색양파 추출물과 어성초 추출물을 0.01%, 0.05%, 0.1%, 0.5%로 처리하였을 때, 피부장벽 조절인자인 filaggrin과 SPT의 발현을 농도 의존적으로 증가시켰다. 반면, 일반양파 추출물도 농도 의존적으로 filaggrin과 SPT발현양을 증가시켰지만, 자색양파 추출물에 비하여 발현양이 많지 않았다. 4. 피부 염증 반응 인자에 미치는 영향을 알아보기 위해서 COX-2의 발현과 AP-1의 발현을 분석한 결과, 자색양파와 어성초의 0.01%, 0.05%, 0.1%, 0.5% 추출물에서 농도 의존적으로 COX-2의 발현과 AP-1의 발현이 감소하였다. 반면 일반양파 추출물도 농도 의존적으로 COX-2의 발현과 AP-1의 발현이 감소하였으나, 자색 양파 추출물에 비하여 감소양이 적었다. 5. 자색양파 추출물과 어성초 추출물은 피부 장벽기능 조절인자인 filaggrin과 SPT의 발현양을 증가시켜 줌으로써, 피부의 수분 유지 기능을 강화하였고, 농도 의존적으로 피부 염증 반응 인자도 감소시켜 항염증효과가 있어, 피부미용기능재료로서의 가능성 보여주었다.

大敦·湧泉 捻轉 및 迎隨 補瀉手技法이 2K1C에 의하여 誘發된 高血壓 白鼠에 미치는 影響

윤대환,한정희,나창수,김선희 경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.3

Objectives : The purpose of this study was to examine effects of acupuncture by needle manipulation at LR1KI1 on the blood pressure, plasma levels of ANP(atrial natriuretic peptide), renin and cardiomegalic index in hypertensive rat induced by two kidney one clip (2K1C).Material and methods : The groups divided into 7 groups; Control, no treatment. Acu-1, acupuncture at LR1KI1 bilaterally and the needle was twirled and rotated forward with the thumb of the right hand 6 times. Acu-2, acupuncture at LR1KI1 bilaterally and the needle was twirled and rotated forward with the forefinger of the right hand 6 times. Acu-3, acupuncture at LR1KI1 bilaterally and the needle was inserted in the opposite direction(body direction) as the channel runs, Acu-4, acupuncture at LR1KI1 bilaterally, the needle was inserted in the opposite direction(body direction) as the channel runs and the needle was twirled and rotated forward with the forefinger of the right hand 6 times. The acupuncture treatments by needle manipulation were started on the 4 week after inducing 2K1C, and executed 5times for 2 weeks. Results : Systolic blood pressure was decreased significantly in Acu-2, Acu-3 and Acu-4. Plasma renin was decreased significantly in Acu-2 and Acu-4. Plasma ANP was increased significantly in Acu-2, however was decreased significantly in Acu-4. Cardiac hypertrophy index was decreased significantly in Acu-1, Acu-2, Acu-3 and Acu-4.Conclusions : These results suggest that acupuncture by needle manipulation at LR1KI1 is effective in hypertension by renal blood problem.

合谷 復溜 補瀉 施術이 少汗에 미치는 影響(Ⅱ)

윤대환,나창수,최찬헌,김영선,김왕인 경락경혈학회 2010 Korean Journal of Acupuncture Vol.27 No.2

Objectives : The purpose of this study is to identify the effect of tonifying or sedative manipulation on LI4 and KI7 for sweet deficiency under cold environment. Methods : The participants were divided into 4 groups; intact group without acupuncture(Intact), acupuncture group inserting straightly with LI4 & KI7 method((LI4-straight⋅KI7-straight), acupuncture group inserting⋅twisting with LI4 tonificaton & KI7 tonificaton method(LI4-tonify ⋅KI7-tonify) and acupuncture group inserting⋅twisting with LI4 sedation & KI7 sedation method(LI4-purge⋅KI7-purge). We obtained the baseline data under cold condition with the temperature 14±1℃. After making the participant staying under cold condition for 10 minute, we measured sweating rate on skin surface and biological responses such as body temperature, oxygen saturation, pulse rate, systolic blood pressure, diastolic blood pressure, deoxy-Hb and oxy-Hb in subcutaneous. Results : The sweating rate on skin surface was not different in all treatment groups compared to the intact group. The body temperature was significantly increased in LI4-straight⋅KI7-straight group compared to the intact group. The oxygen saturation significantly increased in LI4-purge⋅KI7-purge group compared to the intact group. The systolic blood pressure was significantly increased in LI4-purge⋅KI7-purge group compared to the intact group. The deoxy-Hb was significantly decreased in LI4-purge⋅KI7-purge group compared to the intact group. Conclusions : The above results, the effect of LI4, KI7 acupuncture due to the tonifying or sedative manipulation on sweating control could be observed. However, other biological responses with the acupuncture of tonifying or sedative manipulation were giving different expressions. We suggest that continued research of tonifying or sedative manipulation is of importance order to find these expressions.

고지방식이(高脂肪食餌)로 유발(誘發)된 비만백서(肥滿白鼠)모델에서 창출(蒼朮), 의이인(薏苡仁), 마황(麻黃), 창출합의이인(蒼朮合薏苡仁) 마황합록차(麻黃合綠茶) 약침(藥鍼)이 혈청지질(血淸脂質) 및 간기능(肝機能)에 미치는 영향(影響)

윤대환,강자돈,주준성,채우석,나창수,Youn, Dae-Hwan,Kang, Ja-Don,Joo, Joon-Sung,Chae, Woo-Seok,Na, Chang-Su 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.1

Objective : This research was performed to investigate the effect of herbal acupuncture(Atratylodes japonica, Coix lachrymajobi, Ephedra sinica, Atratylodes japonica mixed with Coix lachrymajobi and Ephedra sinica mixed with Green tea) at Pungnyung(ST40) and Umnungchon(SP9) on weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL to total cholesterol ratio of rats fed high fat diet for 5weeks. Method : Experimental groups were divided into normal group(Normal), high fat diet group(Control), high fat diet and Atractylodes japonica-herbal acupuncture group(AJ), high fat diet and Coix lachrymajobi-herbal acupuncture group(CL), high fat diet and Ephedra sinica-herbaI acupuncture group(ES), high fat diet and Atractylodes japonica+Coix lachrymajobi-herbal acupuncture group(AJ+CL), Ephedra sinica+Green tea-herbal acupuncture group(ES+GT). Herbal acupuncture was bilaterally treated at the level of 132.5mg/kg body weight per 2day. Results : Body weight and food efficiency were decreased in AJ, ES, AJ+CL, ES+GT. The level of serum total cholesterol, triglyceride and free fatty acid were increased in AJ, ES, ES+GT. That of serum HDL-cholesterol was increased in AJ. The change of food intake, the level of serum phospholipid and ALP were not significant. The HDL to Total cholesterol ratio was increased in AJ and ES. Conclusion : Atractylodes japonica-herbal acupuncture in ST40 SP9 is effective on Body weight, food efficiency ratio, the level of serum lipid, protection of liver function and prevention cardiovascular risk by obesity induced by high fat diet. Herbal acupuncture mixed Ephedra sinica with Green tea can control the body weight, food efficiency ratio and the level of serum lipid.

三陰交, 陰陵泉, 膀胱兪 單獨 및 配穴 艾灸 施術이 Uranyl Acetate에 의하여 誘發된 白鼠의 急性腎不全에 미치는 影響

윤대환,박종승,김용성,김정상,나창수 경락경혈학회 2003 Korean Journal of Acupuncture Vol.20 No.2