항응고성의 2-Chloro-3-(N-Arylamino)-1,4-Naphthoquinone 유도체 합성

유충규,Ryu, Chung-Kyu 대한약학회 1988 약학회지 Vol.32 No.4

Naphthoquinone derivatives have been found to be anticoagulant. In this report, several new 2-chloro-3-(N-arylamino)-naphthoquinone derivatives were synthesized in oder to develope mild anticoagulant. 2, 3-dichloro-1, 4-naphthoquinone was reacted with p-aminobenzoic acid, m-aminobenzoic acid, toluidine, m-nitroaniline, sulfanilamide, sulfathiazole, sulfaguanidine, phenetidine, 2-aminopyrimidine and 3-amino-5-methylisoxazole in EtOH or AcOH afford 2-chloro-3-(p-carboxy anilino)-naphthoquinone (1), 2-chloro-3-(m-carboxyanilino)-naphthoquinone (2),2-chloro-3-(toluidino)-naphthoquinone (3),2-chloro-3-(m-nitroanilino)-naphthoquinone (4), 2-chloro-3-(4-sulfanilanilino)-naphthoquinone (5), 2-chloro-3-(4-sulfathiazolino)-naphthoquinone (6),2-chloro-3-(4-sulfaguanidino)-naphthoquinone (7),2-chlro-3-(phenetidino)-naphthoquinone (8), 2-chloro-3-(pyrimidine-2-amino)-naphthoquinone (9) and 2-chloro-3-(5-methylisoxazole-3-amino)-naphthoquinone (10) in good yield.

항진균성 6-[(N-Halophenyl)amino]-7-Chloro-5 , 8-Quinolinedione 의 유전독성 평가

유충규(Chung Kyu Ryu),허문영(Moon Young Heo),윤여표(Yeo Pyo Yun) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.3

The clastogenecity and mutagenicity of antifungal 6-[(N-halophenyl)amino]-7-chloro-5,8-quinolinedione (RCK 3, 7, 13, 14, and 15) had been evaluated. Salmonella typhimurium reversion assay (Ames test) was used to test the mutagenicity of RCKs. RCK14 was mutagenic in S. typhimurium(TA98 and TA100) with and without rat liver microsomal activation. Whereas RCK3, 7, 13 and 15 were negative in Ames test with Salmonella typhimurium(TA98 and TA100). The clastogenecity was tested on the RCKs with in vivo mouse micronucleus assay. All of RCKs tested did not show any clastogenic effect in mouse peripheral blood. Thus RCKs were not supposed to cause any chromosomal damage termed micronuclei. These results indicate that RCK 3, 7, 13 and 15 have no genotoxic potential under these experimental condition.

p-Mentha-8-ene-2-one계 향료합성

유충규(Chung Kyu Ryu),송기춘(Ki Choon Song) 대한약학회 1991 약학회지 Vol.35 No.4

The synthetic product of 1-hydroxymethyl-p-mentha-8-ene-2-one was afforded by the reaction between dihydrocarvone and formaldehyde. This reaction involves the aldol condensation. The preferential position of formaldehyde is methyl substituted alpha-carbon atom where these enols are regiospecifically formed. The hydroxymethylation of dihydrocarvone was also proved to happen regiospecifically in the position of alpha-methyl substituted ketone. When 1-hydroxymethyl-p-mentha-8-ene-2-one reacted with LiAlH4, 1-hydroxymethyl-p-mentha-8-ene-2beta-ol obtained. 1-Hydroxymethyl-p-mentha-8-ene-2-one reacted with PDC and chromic acid to give 1-formyl-p-mentha-8-ene-2-one and 1-carboxy-p-mentha-8-ene-2-one. When the hydroxymethyl group of 1-hydroxymethyl-p-mentha-8-ene-2-one was reducted to methyl group, 1-methyl-p-mentha-8-ene-2-one was obtained. Some of these new compound have certain odor. I, II have woody aroma and IV, V have camphory odors. IX has flowery minty odor.

1,4-나프로퀴논 유도체의 항균 및 항진균 작용

유충규(Chung Kyu Ryu),류재천(Jae Chun Ryu),정세영(Sae Young Chung),김동현(Dong Hyun Kim) 대한약학회 1992 약학회지 Vol.36 No.2

In order to evaluate the antimicrobial effect of 2,3-substituted-1,4-naphthoquinone derivatives, we newly synthesized several 2-chloro, 2-bromo and 2-hydroxy-1,4-naphthoquinones and subjected to antibacterial and antifungal activities, in vitro, against Escherichia coli HIHJ, Staphylococcus aureus ATCC6538p, Candida albicans 10231, Aspergillus niger 1231 andTricophyton mentagrophytes 6085. Among these derivatives 3,9,18 and 23 showed the potent antibacterial activities. 18,23 and 28 have the antifungal activities. However, these compounds have no significant hemolytic activity at concentrations higher than that required for showing the antibacterial and antifungal activities.

6-[(N-3,4-디플루오로페닐)아미노]-7-클로로-5,8-퀴놀린디온의 항진균작용 및 안전성 평가

유충규(Chung Kyu Ryu),김동현(Dong Hyun Kim),윤여표(Yeo Pyo Yun),이병무(Byung Mu Lee),허문영(Moon Young Heo),정해문(Hae Moon Chung),권상미(Sang Mee Kwon),정성희(Sung Hee Jung) 대한약학회 1996 약학회지 Vol.40 No.5

6-[(N-3,4-Difluorophenyl)amino]-7-chloro-5,8-quinolinedione(RCK4) was tested for antifungal activities, against systemic infections with Candida albicans in normal mice. The therapeutic potential of RCK4 had been assessed in comparison with ketoconazole and fluconazole. RCK4 had ED50, 0.30 +/- 0.14mg/kg but ketoconazole and fluconazole had ED50, 8.00 +/- 0.73, 10.00 +/- 0.43mg/kg respectively. Intraperitoneally administered RCK3 at the ED50 for 7 days and 14 days reduced Candida albicans colony count in the kidneys and liver as well as ketoconazole and fluconazole at these ED50. And administered RCK4 at the ED50 for 14 days improved survival rates as well as ketoconazole. Acute oral toxicity studies of RCK4 were carried out in ICR mice of both sexes. These acute oral toxicities of RCK4 were low and LD50 values were over 2,850mg/kg in ICR mice. The genotoxicities of RCK4 had been evaluated. RCK4 was negative in Ames test with Salmonella typhimurium and chromosomal aberration test in CHL cells. The clastogenicity was tested on the RCK4 with in vivo mouse micronucleus assay. RCK4 did not show any clastogenic effect in mouse peripheral blood and was negative in mouse micronucleus assay. These results indicate that RCK4 has no genotoxic potential under these experimental conditions.

항응고성의 3-(N-Arylamino)-1,4-Naphthoquinone 유도체 합성(III)

유충규(Chung Kyu Ryu) 대한약학회 1990 약학회지 Vol.34 No.6

2,3-Dibromo-1.4-naphthoquinone was reacted with p-aminobenzoic acid, 2-aminopyridine, 2-amino-4-methylpyridine, m-nitroaniline, sulfathiazol, p-chloroaniline, phenetidine and 2-bromo-3-(N-arylamino)-1,4-naphthoquinones(1 - 8). 2,3-Epoxy-2,3-dihydro-1,4-naphthoquinone was also reacted with p-amonobenzoic acid, p-toluidine, p-chloroaniline, m-chloroaniline, m-nitroaniline, p-phenetidine, N,N-dimethyl-1,4-pheylenediamine as a ring opening and dehydrogenation to form 2-hydroxy-3-(N-arylamino)-naphthoquinones(9 - 16) in good yield. These new compounds(1 - 16) are expected to have a biological activities such as anticoagulant and cytotoxic.

항응고성의 3-(N-Arylamino)-1,4-Naphthoquinone 유도체 합성(II)

유충규(Chung Kyu Ryu),오재돈(Jae Don Oh),서명은(Myung Eun Suh) 대한약학회 1989 약학회지 Vol.33 No.5

2,3-Dichloro-1,4-naphthoquinone was reacted with o-fluoroaniline, p-sulfadiazine, p-acetoanline, N,N-dimethyl-1,4-phenylenediamine as a nucleophilic substitution to form 2-chloro-3-(N-arylamino)-1,4-naphthoquinones (l.-6.) in good yield. 2,3-Dibromo-1,4-naphthoquinone was also reacted with o-fluoroaniline, m-aminobenzoic acid, m-chloroaniline, morpholine, p-acetoaniline, N,N-dimethyl-1,4-phenylenediamine as a nucleophilic substitution to give 2-bromo-3-(N-arylamino)-1,4-naphthoquinones (7.-12.). These new compounds are expected to have a biological activities such as anticoagulant, cytotoxic.

6-[(N-4-클로로페닐)아미노]-7-클로로-5,8-퀴놀린디온의 in vivo 항진균 작용 및 독성 평가

유충규(Chung Kyu Ryu),김동현(Dong Hyun Kim),윤여표(Yeo Pyo Yun),이병무(Byung Mu Lee),허문영(Moon Young Heo),장성재(Seung Jae Jang),김효정(Hyo Jung Kim),박윤미(Yun Mi Park) 대한약학회 1995 약학회지 Vol.39 No.4

6-[(N-4-Chlorophenyl)amino]-7-chloro-5,8-quinolinedione (RCK20) was tested for antifungal activities, in vivo, against Candida albicans. RCK20 was compared with ketoconazole and fluconazole in the treatment of systemic infection with Candida albicans in normal rats. The therapeutic potential of RCK20 had been assessed by evaluating their activities (survival rate) against systemic infections with in normal mice with Candida albicans. RCK20 improved survival rates as well as ketoconazole. RCK20 had ED50, 0.25 +/- 0.18mg/kg but ketoconazole and fluconazole had ED50, 8.00 +/- 0.73, 10 +/- 0.43mg/kg respectively. Activities of RCK20 showed superior to that of ketoconazole and fluconazole. Intraperitoneally administered RCK20 at the ED50, 0.25mg/kg for 7 days and 14 days reduced Candida albicans colony count in the kidneys and livers as well as ketoconazole and fluconazole at these ED50, 8.00 and 10mg/kg. Acute oral toxicity studies of RCK20 were carried out in ICR mice of both sexes. These acute oral toxicities of RCK20 were low and LD50 values were over 2,850mg/kg in ICR mice. The Genotoxicities of RCK20 had been evaluated. RCK20 was negative in Ames test with Salmonella typhimurium(TA98 and TA1OO). The clastogenicity was tested on the RCK20 with in vivo mouse micronucleus assay. RCK20 did not show any clastogenic effect in mouse peripheral blood and was negative in mouse micronucleus assay. These results indicate that RCK20 has no genotoxic potential under these experimental condition.

원자재 조립.가공과정을 갖는 건설공사 프로세스의 적정 재고관리 방안에 관한 연구

임건순,한승헌,정도영,유충규,최석진,Im, Keon-Soon,Han, Seung-Heon,Jung, Do-Young,Ryu, Chung-Kyu,Choi, Seok-Jin 한국건설관리학회 2008 건설관리 : 한국건설관리학회 학회지 Vol.9 No.1

건설현장에서는 일반적으로 많은 양의 자재재고를 보유해 왔다. 이러한 자재재고는 현장의 불확실한 수요에 대응하여 공정지연을 방지하고, 원자재 가격 상승으로 인한 비용증가에 대비하는 등 순기능적 측면이 있다. 하지만 과도한 자재재고는 재고보유에 소요되는 재고유지비를 증가시키고 현장물류 공간배치의 효율성을 저하시키는 등 낭비요인으로 작용하기도 한다. 따라서 현장의 다양한 변동성을 고려하여 적정 자재재고 수준을 결정하고 관리하는 것은 건설공사의 효율성 측면에서 매우 중요한 요소이다. 본 연구는 시스템 접근방법의 절차에 의하여 철근가공 조립공정과 같이 현장에 투입되기 이전에 원자재 조립 가공과정을 갖는 건설 프로세스론 분석하고 이러한 흐름과정에서 재고수준을 적정화하는 알고리즘을 제시하였다. 이러한 재고관리 방안의 적용성을 확인하기 인하여 수도권지역 경전철 건설사업의 철근 가공장에서의 재고관리 사례를 분석하였다. 본 연구에서 제안한 알고리즘을 적용한 결과, 현장의 실제 철근 소요량과 가공장에 입고되는 철근 원자재의 입고량이 균형을 이루었고, 이에 따라 평균 재고량과 재고유시비용이 크게 감소되는 것으로 나타났다. There are usually plenty of material inventories in a construction site. More inventories can meet unexpected demands, and also they may have an economical advantage by avoiding a probable escalation of raw material costs. On the other hand, these inventories also cause negative aspects to increase costs for storing redundant inventory as well as decreasing construction productivity. Therefore, a proper method of deciding an optimal level of material inventories while considering dynamic variations of resources under uncertainty is very crucial for the economical efficiency of construction projects. This research presents a stochastic modelling method for construction operations, particularly targeting a work process involving on-site fabrication of raw materials like iron-rebar process (delivery, cut and assembly, and placement). To develop the model, we apply the concept of factory physics to depict the overall components of a system. Then, an optimal inventory management model is devised to support purchase decisions where users can make timely actions on how much to order and when to buy raw materials. Also, optimal time lag, which minimizes the storage time for pre-assembled materials, is obtained. To verify this method, a real case is applied to elicit an optimal amount of inventory and time lag. It is found that average values as well as variability of inventory level decreased significantly so as to minimize economic costs related to inventory management under uncertain project condition.

소적혈구로부터 새로운 헝태의 세포질 포스포리팍제 A2의 동정 및 특성규명

신혜숙(Hae Sook Shin),김하동(Ha Dong Kim),장동훈(Dong Hoon Chang),전형준(Hyung Jun Jeon),유충규(Chung Kyu Ryu),정성윤(Sung Yun Jung),김대경(Dae Kyong Kim) 대한약학회 2001 약학회지 Vol.45 No.4

A Ca2+_dependent PLA2 activity termed Rpla2, was detected in the cytosol of bovine red blood cells (RBCs). The Rplab was characterized as a similar form to Group IV Cpla2, but different in secteral column chromatographic profiles including an anion exchange column.73 examine whether this Rpla2 is different from the well characterized Rpla2, a quinone derivative, BJ5O, was developed and tested for the inhibitory effect on the two PLA2 enzymes. The rPLA2 activity was inhibited by a quinone derivative (BJ5O) with ID50 of 20μM., but had no effect on the cPLA2, suggesting that rPLA2 may be a novel type of cytosolic PLA2 in RBCs.