측두하악관절에서의 골관절염 유도 동물모델 비교연구

유선녕,이영철,박혜련,유미현,전혜미,김광연,김상헌,옥수민,고명연,안용우,안순철,정성희,Yu, Sun-Nyoung,Yi, Young-Chul,Park, Hae-Ryoun,Ryu, Mi-Heon,Jeon, Hye-Mi,Kim, Kwang-Youn,Kim, Sang-Hun,Ok, Soo-Min,Ko, Myung-Yun,Ahn, Yong-Woo,Ahn, Soon- 대한안면통증구강내과학회 2011 Journal of Oral Medicine and Pain Vol.36 No.4

Osteoarthritis in patients with temporomandibular disorders(TMDs) induces pain, limitation of mouth opening, occlusal problems, and most commonly affects their life quality. Control method and progressive process of osteoarthritis are being extensively researched. The researchers focus on histologic changes, synovial changes, muscular and ligamental changes and observed reaction to pain. Therefore most of them developed the animal model for osteoarthritis in TMD patients. In this study, we applied several methods which induces osteoarthritis of temporomandibular joint(TMJ) in rats or mice. For locally induce osteoarthritis in TMJ, Monosodium iodoacetate(MIA) or interleukin-$1{\alpha}$(IL-$1{\alpha}$) were injected into TMJ joint space for 5 or 3 weeks. Other groups are chosen for osteoarthritis under systemic control including hormonal changes and aging. To observe cellular change, increased collagen, degenerative bony destruction and distribution of proteoglycans (PGs), safranin-O staining and Masson's trichrome staining were used.

Euglena gracilis 추출물의 면역조절 및 생리활성 분석

유선녕(Sun Nyoung Yu),박보배(Bo Bae Park),김지원(Ji Won Kim),황유림(You Lim Hwang),김상헌(Sang Hun Kim),김선아(Sunah Kim),이태호(Taeho Lee),안순철(Soon Cheol Ahn) 한국생명과학회 2021 생명과학회지 Vol.31 No.2

본 연구에서는 다양한 생리활성을 나타내는 compound를 함유하고 있는 것으로 알려진 Euglena gracilis를 hot water로 추출하고(HWE), 그 잔존물을 methanol로 추출(HWME)한 후, murine macrophage RAW 264.7 cell에 처리하여 면역활성을 측정하였다. Murine macrophage RAW 264.7 cell에서 HWE 처리로 IL-1β와 TNF-α가 농도의존적으로 증가되었고, lipopolysaccharide에 의해 유도되는 IL-6와 TNF-α 생성이 HWME 처리에 의해 유의적으로 억제되는 것을 확인하였다. 또한 α-glucosidase, protein tyrosine phosphatase (PTP1B), tyrosinase, xanthine oxidase(XO), angiotensin converting enzyme (ACE) 등에 대한 저해활성을 조사하였다. 그 결과, E. gracilis HWE 추출물에서는 α-glucosidase, tyrosinase, ACE에서 약한 억제 활성을 보였으나, HWME 추출물에서는 PTP1B와 XO에서 유의적인 억제활성을 나타냈다. 따라서 본 연구를 통하여 E. gracilis 추출물의 면역조절 활성 뿐 만 아니라 당뇨와 심혈관 질환에 대한 유의적인 억제활성을 통해 다양한 건강기능성 식품의 소재로 활용될 수 있을 것으로 사료된다. Euglena gracilis is a microalga of great biotechnological interest that can create high levels of bioactive compounds, such as tocopherol, paramylon, and folic acid. The objective of this study was to investigate the biological activities of extracts from E. gracilis, especially those focused on immunological activity. E. gracilis biomass was extracted with hot water (HWE) and the remaining pellet was continuously extracted with methanol (HWME). First, we examined the effect of two extracts from E. gracilis on the production of nitric oxide (NO) and the expression of pro-inflammation cytokines, including IL-1β, IL-6, and TNF-α in murine macrophage RAW 264.7 cells. HWE treatment dose-dependently increased the production of IL-1β and TNF-α. On the other hand, treatment with HWME significantly decreased the generation of NO and pro-inflammatory cytokines (IL-6 and TNF-α) in lipopolysaccharide (LPS)-stimulated macrophage cells. In addition, other biological activities of the extracts were further analyzed: α-glucosidase inhibition, protein tyrosine phosphatase (PTP1B) inhibition, tyrosinase inhibition, xanthine oxidase (XO) inhibition, and angiotensin-converting enzyme (ACE) inhibition. Analysis of these biological activities showed that HWE has more inhibitory effects than HWME against α-glucosidase, tyrosinase, and XO agents. However, the inhibition of PTP1B and ACE with HWME were higher than with HWE. Taken together, the results suggested that E. gracilis possesses various biological activities-especially immunological capabilities-through regulation of cytokine production. Therefore, E. gracilis extract may be potentially useful for food material with immune-regulating effects.

Effect of Trans-unsaturated Fatty Acid on Serum Lipid Levels in Mice

Sun Nyoung(유선녕),Jeong Bin Ahn(안정빈),EunYoung Park(박은영),Sun Jung Lee(이선정),Min Gi Tak(탁민지),Kwang Youn Kim(김광연),Sang Hun Kim(김상헌),Ki Dae Kim(김기대),Soon Cheol Ahn(안순철) 한국생명과학회 2012 생명과학회지 Vol.22 No.8

본 연구에서는 각기 다른 형태의 지방산이 ICR mice의 혈중 지질 농도에 미치는 영향을 평가하고자 하였다. 이를 위하여 8주령 수컷 ICR mice를 일반 식이섭취군(C), 10%의 트랜스 불포화 지방산 섭취군(TFA-1), 30% 트랜스 불포화 지방산 섭취군(TFA-2), 50% 트랜스 불포화 지방산 섭취군(TFA-3), 50% 포화 지방산 섭취군(SFA), 불포화 지방산 섭취군(USFA) 으로 나누어 식이 하였다. 혈중 총 콜레스테롤의 수치는 TFA-3 군과 SFA 군의 지질함량이 다른 군에 비하여 높았으며, 중성지방지수 역시 높게 나타났다. 총 콜레스테롤과 중성지방수치를 이용하여 나타낸 LDL 콜레스테롤의 경우에도 50%의 트랜스 지방산을 섭취한 군에서 포화 지방산을 섭취한 군과 유사한 결과가 나타났다. 이에 반해, 10%, 30%의 트랜스 지방산을 섭취한 군에서는 일반식이 섭취군과 비슷한 결과가 나타났다. 이는 많은 양의 트랜스 불포화지방산을 짧은 기간 동안 섭취하더라도 포화지방산을 섭취한 것과 유사한 효과를 나타낸다는 것을 의미한다. The purpose of this study was to investigate the effects of trans-unsaturated fatty acid (TFA), saturated fatty acid (SFA) and general unsaturated fatty acid (USFA) on serum lipid levels in ICR mice. Male ICR 8-week-old mice were divided into six groups; the control group (C) fed with normal diet, the TFA-1 group fed with 10% trans-unsaturated fatty acid, the TFA-2 group fed with 30% trans-unsaturated fatty acid, the TFA-3 group fed with 50% trans-unsaturated fatty acid, the SFA group fed with 50% saturated fatty acid, and USFA group fed with 50% general unsaturated fatty acid. The serum total cholesterol of TFA-3 and SFA was higher than those of other fat groups and C. The serum triglyceride (TG) of TFA-3 and SFA showed the highest levels among all of diet groups. Also the serum HDL cholesterol levels of TFA-3 showed the lowest. LDL cholesterol and atherogenic indices (AI) were remarkedly increased in TFA-3 and SFA, as compared with other fat fed groups and C. Taken together with results, the TFA-3 group showed serum lipid levels similar to the SFA and different from the USFA. These results suggest that intake of high level of trans-unsaturated fatty acid increased serum triglyceride, LDL cholesterol and atherogenic indices, which may affect risk on serum lipid level for lipid metabolism syndrome.

A PCR Denaturing Gradient Gel Electrophoresis (DGGE) Analysis of Intestinal Microbiota in Gastric Cancer Patients Taking Anticancer Agents

Sun Nyoung Yu(유선녕),Soon Cheol Ahn(안순철) 한국생명과학회 2017 생명과학회지 Vol.27 No.11

인체의 장내에 존재하는 장내 미생물은 서로 공생 또는 길항 관계를 유지하며 우리 몸의 면역 방어 기전에 중요한 요소로 작용한다. 본 연구는 항암제가 위암 환자의 장내 미생물 생태계에 미치는 영향을 조사 하였다. 항암치료를 받는 환자의 분변에서 genomic DNA를 추출하고, 16S rDNA 유전자에 대한 denaturing gradient gel electrophoresis (DGGE)를 수행하였다. 분석된 균주는 개체간의 차이가 있었으나, 대부분 사람의 장내에 살고 있는 normal flora로 동정되었다. 모든 분변에 존재하는 5 개 밴드의 서열 분석 결과에 의하면 Faecalibacterium prausnitzii, Morganella morganii 및 Uncultured bacterium sp.가 나타났고, 항암제 처리 후 Sphingomonas paucimobilis, Lactobacillus gasseri, Parabacteroides distasonis 및 Enterobacter sp.가 증가하였다. 이 연구에서 probiotic으로 알려진 Bifidobacterium과 Lactobacillus 를 특이적 PCR primer를 이용하여 동정한 결과, 항암제 투여로 인해 Bifidobacterium과 Lactobacillus의 개체군이 현저하게 줄어들어 diarrhea와 같은 부작용의 원인을 예상하게 하며, 장내 생태계의 주요 박테리아 집단에도 중요한 영향을 미치는 것을 알 수 있었다. 이러한 결과는 항암제 투여와 같이 시간의 흐름에 따른 균총의 변화를 시각적으로 모니터링하기 위하여 PCR-DGGE 분석법이 유용하다는 것을 나타낸다. Intestinal microbiota is an important factor in the development of immune defense mechanisms in the human body. Treatments with anticancer agents, such as 5-Fluorouracil, Cisplatin, and Oxaliplatin, significantly change the temporal stability and environment of intestinal bacterial flora. The anticancer treatment chemotherapy often depresses the immune system and induces side effects, such as diarrhea. This study investigated the effects anticancer agents have on the intestinal microbial ecosystems of patients with gastric cancer. An exploration of the diversity and temporal stability of the dominant bacteria was undertaken using a DGGE with the 16S rDNA gene. Researchers collected stool samples from patients zero, two and eight weeks after the patients started chemotherapy. After the treatment with anticancer agents, the bacteria strains Sphingomonas paucimobilis, Lactobacillus gasseri, Parabacteroides distasonis and Enterobacter sp. increased. This study focused on the survival of the beneficial microorganisms Bifidobacterium and Lactobacillus in the intestines of cancer patients. The administration of antigastric cancer agents significantly decreased Lactobacillus and Bifidobacterium populations and only moderately affected the main bacterial groups in the patients" intestinal ecosystems. The results showed the versatility of a cultivation independent–PCR DGGE analysis regarding the visual monitoring of ecological diversity and anticancer agent-induced changes in patients" complex intestinal microbial ecosystems.

Denaturing Gradient Gel Electrophoresis for Detection of Bacterial Population and Diversity in Amniotic Fluids and Neonatal Gastric Fluids

김영돈,유선녕,김승철,안순철 대한신생아학회 2013 Neonatal medicine Vol.20 No.2

Purpose: Intra-amniotic infection (IAI) is often polymicrobial, and the 16S rDNA PCR assay has a major limitation that its interpretation is difficult in the presence of multiple 16S rDNAs. Denaturing gradient gel electrophoresis (DGGE) can overcome this limitation by separating PCR products based on sequence. We performed the DGGE analysis to investigate bacterial preva-lence and diversity in amniotic fluids from pregnant women with preterm births and gastric fluids from their newborns. Methods: DNA was extracted from bacterial cells in amniotic fluid (AF) and gastric fluid (GF) and was amplified with universal 16S rDNA primers. For DGGE analysis, the PCR products were loaded onto polyacrylamide gels that were made with denaturing gradients. Results: Bacterial 16S rDNA was detected by PCR from all AF and GF samples. The bacterial species in AF samples were the following: Lactobacillus reuteri (87.0%), uncultured Enterococcus species (65.2%), Ureaplasma urealyticum (13.0%), and Enterococcus faecalis (4.3%). The bacterial species in GF samples were the following: Lactobacillus reuteri (95.2%), uncultured Enterococcus species (42.9%), and Ureaplasma urealyticum (4.8%). Two or more species were identified from 69.6% of AF and 47.6% of GF samples. Conclusion: We suggest that DGGE analysis allows improved understanding of microbial diversity and community in AF and GF.

One-health Approach in the Post-COVID-19 Era: Focusing on Animal Infection

장혜정,유선녕,권오유,안순철 한국생명과학회 2023 생명과학회지 Vol.33 No.2

To prepare for the threat of a future epidemic in the post-COVID-19 era, research based on the one-health concept (i.e., the health of humans, animals, and the environment as “one”) is essential. Cross-species infections are being identified as a result of the high infection rate and viral load of SARS-CoV-2 in humans. The possibility of transmission of SARS-CoV-2 from humans to mink has been determined. In addition, the transmission of SARS-CoV-2 from humans to cats through contact has been considered possible. The data so far show that livestock and poultry are less likely to be infected with SARS-CoV-2. However, if infections are established through a new mutation, the resulting diseases are expected to have enormous ripple effects on various fields, such as human food security, the economy, and trade. In addition, there are concerns about the endemic prospect of SARS-CoV-2 and the high accessibility of companion animals. This is because the evolution of the virus likely occurs in animal hosts. Once SARS-CoV-2 is established in other species, they might serve as intermediate hosts for the re-emergence of the virus in the human population. Thus, it is necessary to ensure a rapid response to future outbreaks by accumulating research data on the animal infection of SARS-CoV-2. These data can have implications for the development of animal models for vaccines and therapeutics against SARS-CoV-2. Therefore, in this study, epidemiological reviews were analyzed, and response strategies against SARS-CoV-2 infection in animals were presented using the One-health approach.

Correlation between Metal Ions and Cytokines in the Saliva of Patients with Oral Lichenoid Lesions

주혜민,유선녕,안용우,옥수민,안순철,정성희 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.8

Purpose: We aimed to investigate the effect of metal ions from oral prostheses (OPs) released into the saliva of patients with orallichenoid lesions (OLLs). Materials and Methods: Subjects (n=183) were divided into four groups according to the presence or absence of OLL and OP. Concentrations of the metal ions titanium, chromium (Cr), cobalt (Co), nickel (Ni), palladium (Pd), silver (Ag), platinum (Pt),gold (Au), and zirconium (Zr) were measured using a laser-ablation microprobe inductively coupled to a plasma mass spectrometer. Saliva levels of interleukin (IL)-6, IL-1β, IL-8, and tumor necrosis factor-α were detected using an enzyme-linked immunosorbentassay. The reticulation/keratosis, erythema, and ulceration (REU) scoring system was used to assess the severity of OLL. Results: Mean concentrations of IL-6 and IL-8 were statistically higher in OLL patients with OPs. The concentration of Ni washigh in OLL groups. The concentrations of Cr, Ni, and Au ions in the saliva were positively correlated with IL-8. REU scores werepositively correlated with salivary concentrations of IL-6 and IL-8, as well as with concentrations of Cr, Ni, and Au. Conclusion: Increased concentrations of metal ions, especially Ni, in saliva were positively correlated with IL-8 and showed positivecorrelations with the severity of OLL.

침향 추출물의 면역조절 및 생리활성 분석

황유림,김광연,유선녕,박광일,안순철 대한한의학방제학회 2022 大韓韓醫學方劑學會誌 Vol.30 No.4

Objectives : Aquilaria crassna is a traditional herbal medicine, which is used to treat allergies, diabetes, neurological diseases. Recently, Aquilaria crassna extracts have been reported in anti-bacterial and anti-inflammatory activities. In this study, various solvents fraction of Aquilaria crassna were investigated on various physiological activities. Methods : According to the polarity, the solvents fraction of Aquilaria crassna were confirmed through TLC, and the activities of the extracts were confirmed in anti-diabetes, anti-obesity, whitening, anti-gout, and anti-inflammation. Results : TLC results showed that ACM and ACM/E have similar patterns and most of the components were transferred to ACM/E. Treatment with ACM and ACM/E fraction were significantly decreased the generation of NO in lipopolysaccharide (LPS)-stimulated macrophage cells. Analysis of biological activities such as α-glucosidase, protein tyrosine phosphatase (PTP1B), tyrosinase, xanthine oxidase (XO) and pancreatic lipase inhibition, showed that ACM and ACM/E have more inhibitory effects than other fractions. Conclusions : Therefore, the results of the present study clearly demonstrate that Aquilaria crassna and its constituents might be beneficial in the prevention or treatment of immune-regulating effects.

Piplartine과 전립선암 세포사멸

공은희,김광연,유선녕,이선이,안순철 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Background: Prostate cancer is one of the leading causes of cancer-related deaths in men worldwide.Piper species have known anti-proliferation and anti-cancer activity. This study was to examine the anti-proliferation effects of piplartine on human prostate cancer cell. Methods: PC-3 human prostate cancer cells received piplartine 15 uM for 24 hours. Microarray was performed on piplartine-treated PC-3 cells. Results: Among 253 genes which were altered by piplartine-treated PC-3 cells (>3-4 folds), 143 genes were up-regulated and 110 genes were down-regulated by piplartine in microarray. Most of genes whichwere up-regulated by piplartine were functionally classified as apoptosis, immune response related genes. Genes which were down-regulated by piplartine were functionally classified as transcription, cell cycle related genes. Conclusion: Piplartine may induce anti-tumor activity for human prostate cancer along with regulated of cell cycle and apoptotic genes in piplartine-induced PC-cells.

항당뇨 효능이 있는 천연물의 탐색 및 활성물질의 분석

이화신,박보배,유선녕,김민지,배윤진,이이룬,이예은,김시윤,심윤호,안순철 한국생명과학회 2023 생명과학회지 Vol.33 No.10

Modern people have an increased incidence of metabolic diseases due to changed eating habits, and diabetes is considered the most significant metabolic disease. Given that existing diabetes treatments are accompanied by side effects, the aim of this study was to identify traditional natural products that have anti-diabetic activity. The potential anti-diabetic and antioxidant activities of natural products were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, α-glucosidase assay, and protein tyrosine phosphatase 1B (PTP1B) inhibition assay. Methanol extracts of Ulmus davidiana var. japonica, Acer tegmentosum branches, Nelumbo nucifera seeds, and Carthamus tinctorius seeds were found to have high anti-diabetic activity and further fractionated with solvents using ethyl acetate and butanol. Consequently, the ethyl acetate fraction of C. tinctorius seeds (MG-11-E) with high α-glucosidase and PTP1B inhibitory activity was selected. MG-11-E was subjected to preparative thin layer chromatography, and fraction #6 showed high α- glucosidase and PTP1B inhibitory activity. Fraction #6 was analyzed and fractionated via high performance liquid chromatography with 50% methanol as the mobile phase, and anti-diabetic activity was observed in the sample that eluted after 4 min as a single peak. The α-glucosidase inhibitory activity exhibited by this sample seemed to be greater than the PTP1B inhibitory activity; thus, it was concluded that a greater anti-diabetic therapeutic effect may be achieved by combining this agent with natural products that inhibit PTP1B activity.