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대전 지역의 공단 폐수에서 분리한 수은과 카드뮴 내성 세균의 분리 및 특성
유경만,전희근,박준봉,이인수,박진숙,하남주,박용근 한국환경과학회 1997 한국환경과학회지 Vol.6 No.3
Mercury- and cadmium-resistant bacteria were isolated from an industrial complex wastewater of Taejon area. All of them were motile, gram negative rods. In the results of physicochemical test and VITEK card test, HMI was identified with Acinetobacter calcoaceticus, CM3 was identified with Comamonas acidovorans, HM2, HM3, CM1, and CM4 were Pseudomonas sp., but HM4 and CM2 were unidentified. They were tested for susceptibility to 14 heavy metals. Mercury-resistant bacteria(HM1, HM2, HM3, and HM4) were sensitive to low concentration(100∼400ppm) of Cd^2+, Co^2+, Zn^2+, and Ni^2+ while cadmium-resistant bacteria(CM1, CM2, CM3, and CM4) showed resistance up to the high concentration(600∼1,200ppm) of these metal ions. As a result of resistance spectrum test of mercury-resistant bacteria, HM1 was broad-spectrum strain, HM2, HM3, and HM4 were narrow-spectrum strains. Transmission electron microscopic examination of cell wall of HM1 culture grown with and without 100ppm of HgCl_2 showed remarkably morphological abnormalities. In the result of atomic absorption spectrometric analysis of cadmium-resistant bacteria grown at 200ppm of CdCl_2 for 6h, all of them accumulated cadmium(14ppm∼57ppm) in cell. In cadmium-resistant bacteria, CM1, CM2, and CM4 were spared from the inhibitory effect of Cd^2+ by the addition of Mn^2+, CM4 were also spared from the inhibitory effect of Cd^2+ by the addition of Mn^2+ as well as Zn^2+.
유경만,조남륜,이원희,이영록,박용근 ( Kyung Man You,Nam Ryun Cho,Won Hee Lee,Yung Nok Lee,Yong Keun Park ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.2
Twelve mercury-resistant bacterial strains were isolated from Anyangcheon. The mercury-resistant bacteria grown on the LB agar supplemented with 100㎍/㎖ of mercuric chloride were identified as Pseudomonas sp. and Bacillus sp.. All of the mercury-resistant bacteria exhibited resistance to at least one antibiotic. Among them, PY109. PY110, and PY112 were broad-spectrum strains which showed resistance to not only inorganic mercuric compound such as mercuric chloride(HgCl_2). but also organomercurial compound such as phenyl mercuric acetate (PMA). The remaining nine were narrow-spectrum strains resistant only to inorganic mercuric compound. PY102, PY103, and PY113 were resistant to cadmium(Cd^2+) as well as mercury(Hg^2+). Plasmids were detected in all strains of Pseudomonas sp., while all strains of Bacillus sp. except for PY106 had no plasmid. Growth of Pseudomonas sp. PY109 in the presence of mercury started after an extended lag phase. The growth curve of Pseudomonas sp. PY109 dropped to the lowest level as soon as the bacteria came into contact with HgCl_2 and PMA. However, The bacteria preincubated with a low concentration of HgCl_2 were prevented from early death. The decrease of concentration of Hg^2+ in the culture medium during growth of Pseudomonas sp. PY109 was confirmed by the UV-VIS spectrophotometer and the inductively coupled plasma atomic emission spectrophotometer.
유경재(柳京在),한윤영(韓允榮),하주현(河周賢) 서울대학교 교육연구소 2011 아시아교육연구 Vol.12 No.4
본 연구의 목적은 초등학생용 및 중·고등학생용‘KEDI 창의적 인성검사(Creative Personality Scale)’를 개발하고 그 타당성을 검증하기 위한 것이다. 본 연구에서 검증한‘KEDI 창의적 인성검사’는 종래에 논의되어 온 다양한 창의적 인성이론들을 검토하고, 기존의 창의적 인성검사들의 장단점을 활용 및 보완하여, 초ㆍ중고생들의 발달적 특성을 고려한 문항으로 차별화시켜 개발한 검사라는 점에서 의의가 있다. 본 연구에서는 선행 연구 및 기 개발된 창의적 인성검사의 분석을 토대로 새롭게 개발한‘KEDI 창의적 인성검사’의 신뢰도와 타당도를 검증하되, 내적일치도, 구인타당도, 변별 및 수렴타당도, 준거타당도의 측면에서 분석했다. 내적일치도는 초등학생용의 경우 .60이상으로 비교적 양호하게 나타났고, 중ㆍ고등학생용의 경우 판단의 독자성과 사고의 개방성을 제외한 나머지 요인들이 .60 이상으로 비교적 양호하게 나타났다. 확인적 요인분석을 통한 구인타당도 결과는 초등학생용의 경우 주요 적합도지수인 RMSEA(.050), CFI(.904), TLI(.883), IFI(.905)에서 적합한 것으로 나타났다. 또한 중ㆍ고등학생용의 경우 RMSEA(.050), CFI(.838), TLI(.801), IFI(.840)에서 적합한 것으로 나타났다. 또한 하주현의 창의적 인성검사(2000)와의 상관을 통한 준거 타당도 결과도 초등학생용의 경우 .809(P<.01)로 높았고, 중ㆍ고등학생용의 경우 .731(P<.01)로 높게 나와 적합한 것으로 밝혀졌다. The purpose of this study is to verify the validity of KEDI Creative Personality Scale for elementary and secondary school student. The existing creative personality scales(CPS) is outdated by excessive or insufficient items, focused on several limited abilities for adult. But this verified KEDI CPS is not only included comprehensively what have been argued and developed for 10 years about creative personality, but also is differentiated by developmental characteristics of elementary and secondary school student To verify reliability and validity of this KEDI CPS, we analyze internal consistency of scale for reliability and construct validity, convergent and discriminative validity. criterion-related validity. The internal consistency of the scale for elementary school student is relatively higher than .60, and all the factors except ‘the independent decision making’ and ‘the openness of thought’ are relatively higher than .60. The confirmatory factor analysis using AMOS to verify construct validity revealed that the structural equation model including 6 construct validity in KEDI CPS showed fit index at a satisfactory level as follows. The major fit indexes are showed as follows; RMSEA(.05), CFI(.904), TLI(.883), IFI(.905) in the scale for elementary school student, RMSEA(.050), CFI(.838), TLI(.801), IFI(.840) in the scale for secondary school student. Futhermore, to secure criterion-related validity, this KEDI CPS showed highly significant correlations with Hah’s Creative Personality Scale elemantary for r=.809(p<.01), secondary for r=.731(p<.01)).