http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
우성균,백성희,신동훈,김혜선,유영준,조중명,강만식,정진하,U, Seong-Gyun,Baek, Seong-Hui,Sin, Dong-Hun,Kim, Hye-Seon,Yu, Yeong-Jun,Jo, Jung-Myeong,Gang, Man-Sik,Jeong, Jin-Ha The Korean Society for Integrative Biology 1997 Korean journal of biological sciences Vol.1 No.2
We have previously shown that chick muscle extracts contained at least 10 different ubiquitin C-terminal hydrolases (UCHs). In the present studies, one of the enzymes, called UCH-9, was purified by conventional chromatographic procedures using $^{125}l$-labeled ubiquitin-${\alpha}$NH-MHISPPEPESEEEEE HYC (Ub-PESTc) as a substrate. The purified enzyme behaved as a 27-kDa protein under both denaturing and nondenaturing conditions, suggesting that it consists of a single polypeptide chain. It was maximally active at pHs between 7 and 8.5, but showed little or no activity at pH below 6 and above 10. Lice other UCHs, its activity was strongly inhibited by sulfhydryl blocking reagents, such as iodoacetamide, and by Ub-aldehyde. In addition to Ub-PESTc, UCH-9 hydrolyzed Ub-aNH-protein extensions, including Ub-${\alpha}NH$-carboxyl extension protein of 80 amino acids and Ubo-${\alpha}NH$-dihydrofolate reductase. However, this enzyme was not capable of generating free Ub from mono-Ub-${\varepsilon}NH$-protein conjugates and from branched poly-Ub chains that are ligated to proteins through ${\varepsilon}NH$-isopeptide bonds. This enzyme neither could hydrolyze poly-His-tagged di-Ub. These results suggest that UCH-9 may play an important role in production of free Ub and ribosomal proteins from their conjugates.
팽이버섯(Flammulina velutipes) 교잡분리집단의형태학적 특성 분석
우성이,공원식,김은선,장갑열,신평균,오연이,남윤걸,오민지,김경수 한국버섯학회 2015 한국버섯학회지 Vol.13 No.3
This study was conducted to obtain a growth correlation of basal information from the development of disease resistantFlammulina velutipes cultivars through back-crossing between the strains of wild-type brown monokaryon 4019-20 and thederivative of commercial quality white monokaryons 3. The two strains were selected to back-cross for further enhancing their latentattributes and growth characteristics. The parents of 4019-20×M3 back-crossed to reproduce F1, M3-Sn. Using F1, M3-Sn procuredand isolated into 94 monokaryon strains. Further examination of growth characteristics carried out by back-crossing between M3and BC1F1 from M3-n dikaryon. Monokaryon exhibited an irregular growth pattern and demonstrated to be sluggish development inthe sawdust medium. However BC1F1(M3-n) dikaryon strains confirmed mostly regular growth pattern and demonstrated ordinarygrowth in the sawdust medium. The fruitbody of BC1F1 confirmed as light-brown colour to be the dominant gene. The colourdistributions of fruitbody, BC1F1, resulted as follows; 7% of dark brown, 25% of brown , 27% of light brown, 16% of ivory and 25%of white. The ratio of the other color to white showed 3 to 1 which suggested two major genes were related to fruitbody color.
능동질량감쇄기를 이용한 지진하중을 받는 고층빌딩의 제어
김기봉,우성균 中央大學校 建設環境硏究所 1993 環境科學硏究 Vol.4 No.-
A building implemented by the active control system (Active Mass Damper)is analyzed subjected to earthquake excitation. The effetiveness of the active mass damper is studied to reduce coulpled lateral-torsional motions of building. The ground accelation os modeled as a uniformly modulated non-stationary random process. The problom is formulated using the transfer matrices approach in the frequency domain and the closed-loop control law. The random vibration analysis is carried out to determine the statistics of building response and the required active control forces. Numerical example of a 40 story building under earthquake excitation is given to illustrate the significant reduction of the building response by means of the active mass damper control system.
팽이버섯(Flammulina velutipes)의 Genome-wide SNP (Single Nucleotide Polymorphism)에 의한 계통 분석
우성이 ( Sung I Woo ),김은선 ( Eun Seon Kim ),한재구 ( Jae Gu Han ),장갑열 ( Kab Yeul Jang ),신평균 ( Pyung Gyun Shin ),오연이 ( Youn Lee Oh ),오민지 ( Min Ji Oh ),조성환 ( Sung Hwan Jo ),이정희 ( Jeong Hee Lee ),김경수 ( Kyung So 한국균학회 2015 韓國菌學會誌 Vol.43 No.4
Genome-wide reanalyzed data of 25 Flammulina strains were compared against the reference genome (KACC42780) to establish a genome-wide single nucleotide polymorphism (SNP). The rate of mapping differences between the strains reflected in the strain variation in its result. Genome-wide SNPs distribution divided into types of homozygous SNP and heterozygous SNP moreover all of the strains demonstrated a wide variation in all of the regions. In the further study of topological relationship between the collected strains, phylogenetic tree was separated into 3 major groups. Group I contained F. velutipes var. related strains of ASI 4062, 4148, 4195. Group 2 contained strains that are different species of ASI 4188 F. elastica, ASI 4190 F. fennae, and ASI 4194 F. rossica. The other 19 strains F. velutipes were classified as a single group. However, further experiment to discriminate its genetic relationship between the white group and brown group did not verify its validity. The inferred tree exhibited a phylogenetic relationship between Korea white fruitbody forming strains of ASI 4210, 4166, 4178 and Japan white fruitbody forming strains of ASI 4209, 4167 confirmed to be genetically closely related.
잿빛만가닥버섯( Lyophyllum decastes)의 인공재배 특성
우성미,박용환,유영복,신평균,공원식,장갑열,박윤정,성재모 한국버섯학회 2009 한국버섯학회지 Vol.7 No.2
잿빛만가닥버섯의 인공재배법을 개발하고자 자연에서 채 집, 분리된 균주의 배양적 특성을 검정하고 자실체 발생을 위 한 다양한 방법을 수행하였다. 잿빛만가닥버섯의 9균주를 공시하여 선발한 결과, 균사생장이 빠르고 균사밀도가 좋은 SPA 202, SPA 205균주를 선발하였다. 한천배지의 경우는 BC배지에서 가장 양호하였다. 톱밥발효배지에서 인공재배 하였을 때 20-25℃에서 균배양은 종균 접종 후 50일, 복토 후 7일이 소요되었으며, 원기형성 및 초발이는 17-18℃에 서 7-8일이 소요되었다. SPA 202의 수확적기는 갓이 형성 되기 전의 자실체는 갓 60㎜, 대 67㎜ 으로 단단하였고, 인공 재배한 수량은 130g이었다. 인공재배에서 일반톱밥보다 발 효톱밥이 수확량이 86% 증가하였으며 SPA 202가 가장 양 호한 것으로 나타났다.
김은선,우성이,오민지,오연이,신평균,장갑열,공원식,이창수 한국버섯학회 2015 한국버섯학회지 Vol.13 No.3
‘Baekjung’ adaptable to high temperature was made by crossing between monokaryon derived from selfing of brownstrain and monokaryon derived from Korea white strain. In the condition that temperature is maintained at 10oC without lowtemperature of 4oC suppressing treatment and wrapping during cultivation period, it showed good productivity thanUri1ho(control). The optimum temperature of mycelial growth was 30oC and that of fruiting body initiation and developmentwere 14oC and 7oC, respectively. The days for the fruiting and yield were 7days and 277±11.2g per 1,100ml bottle,respectively. This variety needed high concentration of carbon dioxide up to 4,000 ppm for the good quality.
잿빛만가닥버섯(Lyophyllum decastes)의 발효톱밥에 의한 인공재배 특성에 관한 연구
우성미,박용환,유영복,신평균,장갑열,이강효,성재모 한국버섯학회 2009 한국버섯학회지 Vol.7 No.4
These experiments were carried out to determine the optimal culture conditions for nine strains of collected Hatakeshimeji, Lyophyllum decastes (Fr.:Fr.) Sing. SPA 202 and SPA 205 strains were selected because mycelium grew fast and showed fine density. All strains showed fast mycelial growth and mycelial density on BC(Burke compost) media for 20 days of incubation. The optimal sawdust species for the mycelial growth were the fermented sawdusts of Quercus aliena and Populus deltoides . Spawn running period on the fermented sawdust substrate required 50 days at 20 to 25℃ and additional 7 days after soil casing. Cultivation period and temperature forprimordia formation and fruitbody development appeared from 10 to 11 days and from 7 to 8 days at 17 to 18℃ respectively. The length of pilei and stipes of SPA 202 harvested in optimal stage showed 60mm and 67mm, respectively. Yield of SPA 202 strain grown on fermented sawdust substrate was 130g per 1,100㎖ in bottle cultivation. The length of pilei and stipes of SPA 205harvested in optimal stage showed 51mm and 81mm, respectively. Yield of SPA 205 strain grown on fermented sawdust substrate was 129g per 1,100 ㎖ in bottle cultivation. SPA 202 strain and SPA 205 strain in artificial bottle cultivation of Lyophyllum decastes used in fermented sawdust substrate were selected as themost appropriate strain in yield.
잿빛만가닥버섯(Lyophyllum decastes)의 ITS 영역염기서열 및 RAPD에 의한 계통학적 유연관계 분석
우성미,박용환,유영복,신평균,장갑열,진용주,성재모 한국버섯학회 2009 한국버섯학회지 Vol.7 No.3
Phylogenetic relationships of Hypsizygus mamoreus and Lyophyllum decastes artificial cultivated using ITS sequences and RAPD polymorphism have been analyzed. Based on ITS region sequences of 14 strains, we could divide into 2 group as group1 to Hypsizygus mamoreus and the control isolated group2 to Lyophyllum decastes were identified as the same species. Restrict analysis of rDNA ITS region which was amplified by PCR, 14 collected strains could be classified into 4 clusters. There was approximately 58% genetic similarity between clusterⅠ(SPA 100, 101, 102) and clusterⅡ(SPA 200, 208 and SPA 201, 202), 41% between clusterⅢ(SPA 104, 103, 203) and cluster Ⅳ(SPA 204, 206, 207, 205) by BLAST analysis. RAPD polymorphisms were used to analyze the species diversity of artificially cultivated Lyophyllum decastes SPA 202. As a result, similarity between SPA 202 and SPA 203 was 75%, at the same time, similarity between SPA 202 and Pleurotus eryngii SPA 103 and SPA 104 was 65%.
잿빛만가닥버섯( Lyophyllum decastes)의 유연관계 분석
우성미,박용환,유영복,신평균,공원식,장갑열,박윤정,진용주,성재모 한국버섯학회 2009 한국버섯학회지 Vol.7 No.2
인공재배된 공시균주 14개에서 분자생물학적 동정을 확 인하기 위하여 ITS부위의 염기서열을 사용하여 종속간 유연 관계 및 RAPD 다형성을 분석하였다. ITS영역부위 종속간 유연관계는 염기서열의 유사성이 SPA 100, 101, 102은 만 가닥버섯인 Hypsizygus 에 속하였으며, 11균주는 잿빛만가 닥버섯 Lyophyllum decastes 그룹에 속하였고 다시 3개의 subgroup으로 분류되어 속간 유연관계로 분석되었다. 또한 인공재배한 버섯의 종 다양성을 분석하기위해 RAPD를 수행 한 결과 가장 수량이 양호하며 우량계통인 SPA 202는 SPA 203와 75%의 유사성을 보여주었고, Lyophyllum decastes 의 공시균주인 SPA 103과 SPA 104와는 유사성이 65%로 나타났다. 결론적으로, 자연에서 채집하여 인공재배가 확립 된 SPA 202는 분자생물학적 분석 방법에 의해 Lyophyllum decastes 로 추정 할 수 있다.