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김정안,홍창표,김대수,문재우,최유리,외정우,권윤정,이자랑,정이든,배진한,최봉환,고준수,송상훈,안궁,하홍석,양영목,이학교,박경도,한규동,이주미,차희재,Selvam Ayarpadikannan,조병욱,박종화,도경탁,김희수 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.3
Athletic performance is an important criteria used for the selection of superior horses. However, little is known about exercise-related epigenetic processes in the horse. DNA methylation is a key mechanism for regulating gene expression in response to environmental changes. We carried out comparative genomic analysis of genome-wide DNA methylation profiles in the blood samples of two different thoroughbred horses before and after exercise by methylated-DNA immunoprecipitation sequencing (MeDIPSeq). Differentially methylated regions (DMRs) in the preand post-exercise blood samples of superior and inferior horses were identified. Exercise altered the methylationpatterns. After 30 min of exercise, 596 genes were hypomethylated and 715 genes were hypermethylated in the superior horse, whereas in the inferior horse, 868 genes were hypomethylated and 794 genes were hypermethylated. These genes were analyzed based on gene ontology (GO) annotations and the exercise-related pathway patterns in the two horses were compared. After exercise, gene regions related to cell division and adhesion were hypermethylated in the superior horse, whereas regions related to cell signaling and transport were hypermethylated in the inferior horse. Analysis of the distribution of methylated CpG islands confirmed the hypomethylation in the gene-body methylation regions after exercise. The methylation patterns of transposable elements also changed after exercise. Long interspersed nuclear elements (LINEs) showed abundance of DMRs. Collectively, our results serve as a basis to study exercise-based reprogramming of epigenetic traits.