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Genetic Distances of Three Mollusk Species Investigated by PCR Analysis
오현,윤종만 한국발생생물학회 2014 발생과 생식 Vol.18 No.1
Three species of Nortamea concinua (NC) and Haliotis discus hannai (HDH) from Tongyeong and Sulculus diversicolor supertexta (SDS) are widely distributed on the coast of the Yellow Sea, southern sea and Jeju Island in the Korean Peninsula under the innate ecosystem. There is a need to understand the genetic traits and composition of three mollusk species in order to evaluate exactly the patent genetic effect. PCR analysis was performed on DNA samples extracted from a total of 21 individuals using seven decamer oligonucleotides primers. Seven primers were shown to generate the unique shared loci to each species and shared loci by the three species which could be clearly scored. A hierarchical clustering tree was constructed using similarity matrices to generate a dendrogram, which was facilitated by the Systat version 10. 236 specific loci, with an average of 56.3 per primer, were identified in the NC species. 142 specific loci, with an average of 44.7 per primer, were identified in the HDH species. Especially, 126 numbers of shared loci by the three species, with an average of 18 per primer, were observed among the three species. Especially, the decamer primer BION-75 generated 7 unique loci to each species, which were identifying each species, in 700 bp NC species. Interestingly, the primer BION-50detected 42 shared loci by the three species, major and/or minor fragments of sizes 100 bp and 150 bp, respectively, which were identical in all samples. As regards average bandsharing value (BS) results, individuals from HDH species (0.772) exhibited higher bandsharing values than did individuals from NC species (0.655). In this study, the dendrogram obtained by the seven decamer primers indicates three genetic clusters: cluster 1 (CONCINNA 01CONCINNA 07), cluster 2 (HANNAI 08HANNAI 14), cluster 3 (SUPERTEXTA 15SUPERTEXTA 21). Comparatively, individuals of HDH species were fairly closely related to that of SDS species, as shown in the hierarchical dendrogram of genetic distances.
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오현이,강영순 朝鮮大學校 醫學硏究所 1982 The Medical Journal of Chosun University Vol.7 No.-
The data in this article were obtained from a survey of the attitudes and the actual practice of health care among nursing students in Kwangju, Jeonnam, Korea. The survey was carried out during the period of Decemder 1 through 10, 1981. In total, ninety-three female students responded to the questionaire. Results of the survey are : 1. Parasite Control 65.5% of the students were planning to take anthelmintics twice a year, but only 29.0% made it an actual practice when deemed necessary. 46.2% planned to have a stool examination once a year, but in actual practice, 45.2% did not have the test. The results also show that despite their positive attitudes towards parasite control, they did not actually practice it, mostly(61.0%) due to negligence. 2. Dental Care For brushing teeth, the highest percentage (60.2%) of students had the attitudes that brushing three times a day was good, but twice a day was the practice with 62.4% of them. For daily brushing, the highest rated time for brushing was after meals, it was 79.6% in attitudes and 63.4% in practice. For frequency of seeing a dentist, the highest rate of regular consultation was 90.3% in attitudes, and in practice, 54.8% only when necessary. These results show that despite their attitudes, most subjects did not actually practice sound dental hygiene. 3. Smoking Opposite results were obtaindd for smoking with 54.8% opposed to smoking and as high as 93.5% not smoking in practice. 4. Drinking In attitudes 40.9% approved, but in actual practice, 49.5% did not drink at all. In general, they opposed drinking inconsistent with 40.9%. 5. Frequency of X-ray examinations The need for a chest X-ray once a year was held by 79.5%, and in practice, 62.2% had an X-ray taken. The reason for not getting X-rays was that 71.4% of the subjects were either negligent, or felt no need for it. 6. Frequency of Vaccination In attitude, 84.9% considered vaccination necessary for good health, but in practice, 65.6% took them only when it was necessary. The reason was that 71.9% were either negligent, or were not required to be vaccinated. 7. Usage of Medical Care Facilities Initial treatment of illness in an early stage was sought by 44.1% of those surveyed, and the medical care was rendered by a clinic or hospital for 61.2%. 53.8% were advised directly by pharmacists to take medication. Medical care decisions were made with parents in 67.7% of the subjects.
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吳賢一 건국대학교 1981 學術誌 Vol.25 No.1
Die Dichtung entsteht aus der Sehnsucht nach der Wiederkehr zu dem urspru¨nglichen Wesen der Menschen. Der Grundzug der Dichtung ist als eine Phase bezeichnet, in der sich des verborgene Wesen des Ichselbstes kla¨rt. Die Erkenntnis, daβ ich Nichts bin, bird in der Dichtung ausgedru¨ckt, wie wir die menschlichen Grundeigenschaft seven. Je nach der literaturgeschichtlichen Periode habe ich dieses Thema durch die antiken Trago¨dien, Shakes-pear's Trago¨dien, Trago¨diens des deutschen Realismus, Fragmenten von F. Kafka und G. Benn, und Roman von M. Frisch.
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오현이,강영순 조선대학교 1981 The Medical Journal of Chosun University Vol.6 No.-
Evaluation of job satisfaction of nurses is very important for solving the problems which occur in hospital duty and to set up policies of job procedures. This study was undertaken to investigate the responses and the priorities in job satisfaction. This data were collected through questionnaires from 177 nurses working at two hospitals in Kwangju City during the period of December 12~24,1980. The questionnaires developed by the researcher included 46 questions concerned vvith job satisfaction; i. e. professional status, interaction, task requirements, administration, doctor-nurse relationships, Pay, and personal autonomy. The results obtained can be summarized as follows: 1) The response of job satisfaction occording to hospital duty was most positive 63.9% in Hospital A and 53.0% in hospital B for personal autonomy and most negative 26.2% in hospital A and 25.4% in hospital B for administration. 2) The response of job satisfaction. according to educational level was most positive 64.6% for graduates from junior colleges and 61.5% for graduates from four-year colleges for autonomy and most negative junior college 26.1%, four-vear college 24.8% for administration in hospital A. In hospital B, it was most positive 51.8% & 54.6% for interaction and most negative for doctor-unrse relationship among graduates from junior college 24. 4% and for administration among graduates from fouryear colleges 25.9% 3) The response of priority according to each item showed that personal autonomy is considered the most important and administration the least important in bofh hospitals.
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일부 체육고등학교 학생의 자아개념 및 스트레스 요인과 대처방법에 관한 연구
오현이,김혜숙 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.1
The purpose of this study was tried to find out the students stressful life events, their methods to cope with them, and their self-concept effecting on the coping method, to analize the relationship among these three variables, and to provide basic data which maintain and promote student's adaptability and mental health. The data were collected from total 216 students of a physical education high school in Gwansg-ju. The Questionnair survey was done from June 25 to July 5, 1991. The instruments were composed of; 1. A part of Choi's perceptual orientation scale for measuring Self-Perception of students. 2. Rosenberg's Questionnair for measuring student's evaluation of self-esteem. 3. Modified McCubbin's Adolescent-Family Inventory of Life Events and Changes and Adolescent Life Change Event Questionnaire by Oh and Han's Stressful Life Events. 4. Modified Patterson's Adolescent Coping Orientation for Problem Experience by Oh and Han's coping method. The survey questionnaire consisted of 30 questions related to self-concepts, 47 questions related to stressful life events, and 44 questions related to coping method. The collected data were analyzed by descriptive statistics, frequency, ANOVA, t-test, and Pearson correlation coefficient. The results were summarized as follows; 1. The mean score of self concept was 10.58. 2. The mean score of stressful life events was 6.31. 3. The mean score of coping methods was 120.28. 4. The relationship between general characteristics and self-concept represented significant differences in sex(t=3.27, P=0.00), academic score(F=4.81, P=0.00), leisure time(F=4.32, P=0.00), reading(F=3.52, P=0.01), going to college after graduation(F=7.04, P=0.00), and health condition(F=6.39, P=0.00). 5. The relationship between stressful life events and general characteristics represented significant differences in presence or absence of parents (F=3.49, P=0.00), academic score(F=4.29, P=0.00), and family atmosphere(F=-2.74, P=0.01). 6. The relationship between general characteristics and coping methods represented significant differences in religion(F=3.11, P=0.02), educational level of father(F=2.57, P=0.03), and leisure time(F=3.98, P=0.00). 7. The relationship between self-concept and coping methods(r=0.30, P=0.00), and stressful life events and coping methods(r=0.30, P=0.00) represented correlation statistically significant.
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吳現道 제주대학교 1972 논문집 Vol.4 No.-
제주도 Pineapple 栽培開發을 爲하여 生態學的 要因인 氣候的 環境과 土壤條件에 對한 諸般要因을 調査하고 人爲的 開花를 促進하기 爲하여 植物生長調節物質 X-Naphtalene acetic acid와 Acetylene gas의 影響에 對하여 調査한 結果는 다음과 같다. 1. Pineapple 栽培에서 가장 沮害要因을 나타내는 것은 溫度와 日照量이었다. 2. 越冬時 低溫 對策과 日照量 缺乏에 대한 人爲的 開花促進이 반드시 必要하였다. 3. Vinyl house內의 土壤 水分은 겨울동안 크게 變動이 없었다. 4. 日照量 不足에 對한 花芽分化 障碍를 解消하기 爲하여 植物 生長調節 物質을 處理란 結果는 飽和 Acetylene gas 溶液과 L-N.A.A 30ppm 處理區에서 그 效果가 顯著하게 좋았다 This study was performed to investigate ecological factors(Climatological environment and soil conditions), and the effect of plant growth regulators, X-Naphtalene acetic acid and saturated Acetylene solution, for the development Pineapple growing in cheju-do. The obtained results are as following : 1. The most obstructive climatical factors for pineapple growing were the shortage of atmospheric temperature and sunlight. 2. Keeping warm during the winter and promoting the artificial differentiation of flower bud for the shortage of sunlight, were needed. 3. during the winter, soil moisture in the plastic greenhouse was hardly changed. 4. To promote the differentiation of flower bud, the treatment of plant growth regulators, the saturated Acetylene solution and 30ppm plots of X-Naphtalene acetic acid made good effects.
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오현인,Tae-Soo Lim,Gee-Dong Lee,Hyun-Ku Kim 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.2
Response surface methodology was employed to optimize extraction conditions for finding the maximal functional properties of Pleurotus eryngii. Based on central composite design, the study plan was established with variations of microwave power (30-150 W), ethanol concentration (0-99.9%), and extraction time (1-9 min). Regression analysis was applied to obtain a mathematical model. A maximal yield of 47.86% was obtained when the microwave power, ethanol concentration, and extraction time were set at 122.7 W, 42.14%, and 8.3 min, respectively. A maximized electron donating ability of 93.32% was found under the following conditions: a microwave power of 144.19 W, an ethanol concentration of 49.52%,and an extraction time of 6.7 min. Whenthe microwave power, ethanol concentration, and extraction time were set at 125.43 W, 40.54%, and 8.1 min, respectively, the maximum nitrite-scavenging ability was 80.47%. The optimum ranges of the extraction conditions, superimposed by the response surface methodology, couldpredicate a microwave power of 110-150 W, ethanol concentration of 0-45%, and extraction time of 7-9 min.
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