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백서 척수에서 Capsaicin과 Eugenol이 iCGRP (immunoreactive calcitonin gene-related peptide) 분비 조절에 미치는 영향
오원만,김원재,최남기,박상원,황인남,김선현 大韓齒科保存學會 2001 Restorative Dentistry & Endodontics Vol.26 No.5
Neuropeptide such as calcitonin gene-related peptide and substance P may mediate neurogenic inflammation, but little is known about the regulation of neuropeptide release from rat spinal cord. Eugenol has been reported to reduce odontogenic pain and is known to have a structure similar to capsaicin, a potent stimulant of certain nociceptors. This study was done to examine the effect of capsaicin and eugenol on immunoreactive calcitonin gene-related peptide (iCGRP) release from rat spinal cord and whether eugenol regulates capsaicin-sensitive release of iCGRP or it evokes capsaicin-sensitive release of iCGRP. The dorsal half of rat lumbar spinal cord was chopped into 200㎛ slices. They were superfused (500㎕/min) in vitro with an oxygenated Kreb's buffer. The EC50 of capsaicin on iCGRP release was measured. Eugenol (600㎛ and 1.2mM) and vehicle (0.02% 2-hydroxyl-β-cyclodextrin) were administered prior to stimulation of rat lumbar spinal cord with capsaicin. The amount of iCGRP release from rat lumbar spinal cord was measured by radioimmunoassay. The results were as follows : 1.iCGRP release from rat lumbar spinal cord was dependent on concentration of capsaicin. The EC50 of capsaicin on iCGRP release was 3㎛. 2.In the vehicle treated group, capsaicin (3㎛) evoked a 14-fold increase over basal iCGRP level. 3. Administration of 600㎛ and 1.2mM eugenol evoked a 2.2-fold increase and a 2.3-fold increase over basal iCGRP level respectively. 4.Administration of 600㎛ and 1.2mM eugenol increased capsaicin evoked release of iCGRP by more than 50%. These results indicate that eugenol evoke CGRP release from central nervous system and potentiate the pain-inducing action of capsaicin on it Key words : Eugenol, Capsaicin, Rat lumbar spinal cord, Superfusion, Radioimmunoassay
인공타액 오염이 수종 상아질접착제와 상아질간의 결합에 미치는 영향
오원만,양규호,류미애 大韓齒科保存學會 1992 Restorative Dentistry & Endodontics Vol.17 No.2
The purpose of this study was to evaluate the influence of artificial saliva contamination on bonding of several dintin adhesives to dentin. Sixty-three human molar teeth extracted within a month were used. Each tooth was sectioned longitudinally in a buccolingual direction to obtain 126 wpecimens. These specimens were randomly divided into three froups and were treated by Scotchbond 2, Gluam and All vond. Each group was subdivided into three subgroups; normal group not contaminated with artificial saliva, contaminated with artificial saliva and dried group, and contaminated with artificial saliva and washed and dried group. Enamel/dentin bonding agent (Dental Adhesive of Scotchbond 2) was applied light cured on the treated dentin surfaces. Threrafter P-50 were cured on them, and specimens were storde in 37℃ artificial saliva for 24 hours before measuring shear bond strength. Shear bond strengths were determined using an universal testing machine with cross head speed 1mm/min and SEM examinations were conducted to evaluate the resin-dintin interface and degree of penetrating resin string into the dentinal tubules. The following results were obtained. 1. Normal gropqs ont contaminated with artificial saliva showed greater shear bond streagth than any other group contaminated with artificial saliva)P<0.01). 2. The shear bond strengths showed no significant difference between washed groups with distilled water and ont washed groups after contamination with artificial saliva(P>0.05). 3. In normal groups, the shear bond strength of A group was significantly greater than in any other group(P<0.01). 4. In S and G groups, fractrues after shear bond strength tests occured adhesively on resintooth interface in all specimens. But in A groups, fractrue of the normal group occured cohesively in dentin and fractrue of the contaminated groups coourde adhesively and cohesively. 5. On SEM examination, the number of resin strings penotrated into dentinal tubules were the greatest in normal groups, followed by, in descending oredr, wasghed groups and not washed groups after contamination with artificial saliva.
금(金) 광산(鑛山) 광미(鑛尾)로부터 유가자원(有價資源) 회수(回收)에 관한 연구(硏究)
오원,조희찬,이진수,Oh, Won,Cho, Hee-Chan,Lee, Jin-Soo 한국자원리싸이클링학회 2010 資源 리싸이클링 Vol.19 No.6
가행중인 금(gold) 광산에서 발생하는 광미(tailing)로부터 금 및 실리카와 같은 유가자원을 회수하기 위해 선별특성을 검토하였다. 금 선별에는 고비증액(heavy liquid}을 이용한 비중선별(gravity separation)을 하였고, 실리카 선별에는 하이드로싸이클론을 이용한 분급/비중선별, 부유선별(froth-flotation) 그리고 마찰대전형 정전선별 (triboelectrostatic separation)을 하였다. 실험결과 금은 비중액의 비중이 2.72일 때 품위가 최고 5.58 g/ton인 정광을 약 3 wt.% 회수하였으며, $SiO_2$의 함량이 약 94%인 고순도 실리카를 회수할 수 있었다. 따라서, 본 연구를 통하여 환경을 훼손하는 광미의 감량 및 무해화는 물론 유가자원 회수를 위한 공정을 수립 할 수 있을 것으로 기대된다. This study was carried out to develop a process flow sheet for recovering valuables (gold and high purity silica) from the gold mine tailings containing 1.7 g/ton of gold and 79.48 wt.% $SiO_2$. Float-sink tests using heavy liquids was conducted to explore the possibility of recovering gold by gravity separation. Hydrocyclone, froth flotation, and triboelectrostatic separatoin tests were conducted to recover high purity silica from the gold mine tailings. The results of float-sink tests showed that particles containing 5.58 g/ton of gold could be obtained at 2.72 specific gravity, but with very low yields around 3%. Meanwhile, all tests with hydrocyclone, froth flotation, and triboelectrostatic separation showed that high purity silica with $SiO_2$ content over 90% could be obtained. The purity could be improved further up to about 94% by employing several recleaning steps in the froth flotation and triboelectrostatic process.