활천가족실 / 어떤 교회 창립예배의 격려사

오신근 기독교대한성결교회 활천사 1992 활천 Vol.461 No.-

인체 대장암 세포주(HT-29)에서 담즙산 합성유도체(HS-1200)의 세포 사망 기전

오신근,양광모,허원주,유영현,서홍석,이형식 대한방사선종양학회 2002 Radiation Oncology Journal Vol.20 No.4

A Novel Chenodeoxycholic Derivative HS-1200 Induces Apoptosis inHuman HT-29 Colon Cancer CellsSin Geun Oh, M.D.*, Kwang Mo Yang, M.D.*, Won Joo Hur, M.D.*, Young Hyun Yoo, M.D.†, Hong Suk Suh, Ph.D.‡ and Hyung Sik Lee, M.D.*

소아 기관지천식 환자에서 혈액과 타액중의 Theophylline농도의 연관성에 관한 연구

오신근,권순한,윤운기,류한선,손기호,Charles D.Sands 대한천식알레르기학회 1993 천식 및 알레르기 Vol.13 No.1

Theophylline is widely used in Korean Pediatric patients in the treatment of asthma and neonatal apnea. To achieve adequate bronchodilation and avoid side effects, It is generally accepted that the serum theophylline concentration should be maintained between 10 and 20 mcg/ml. The measurement of this serum concentration usually requires a 3-5cc blood sample which on many occasions is repeated several times. In the pediatric population, especially, this exposes the children to multiple venipuncture with its potential associated trauma. To evaluate the reliability of salivary theophylline levels as a guide to serum theophylline levels, we studied the twenty of asthmatic children, who were admitted to our hospital and received intravenous aminophylline and oral sustained release theophylline. The mean theophylline concentration of Group I were 10.11+- 4.89mcg/ml in serum and 6.09+- 3.24mcg/ml in saliva. A strong correlation between the serum and saliva theophvlline levels, r=0.87, was noted and it is statistically significant. Regression Equation was se- rum conc. = 1.3050 X saliva conc. 2.1624. Also there was a good correlation between the predicted and actual theophylline concen trations, r=0.78, and no significant diR'erence between two values in Group II. These suggest that the determination of salivary theophylline concentration may be a convenient, noninvasive method for routine monitoring of serum theophylline concentration.

자동차부품용 KSD 6061소재의 기계가공시 표면거칠기에 관한 실험적 고찰

오신근(S.K. Oh),이주상(J.S. Lee) 한국생산제조학회 2013 한국생산제조시스템학회 학술발표대회 논문집 Vol.2013 No.10

인체 대장암 세포주(HT-29)에서 담즙산 합청유도체(HS-1200)의 세포 사망 기전

오신근(Sin Geun Oh),양광모(Kwang Mo Yang),허원주(Won Joo Hur),유영현(Young Hyun Yoo),서홍석(Hong Suk Suh),이형식(Hyung Sik Lee) 대한방사선종양학회 2002 Radiation Oncology Journal Vol.20 No.4

목적 : 인체 대장암 세포주인 HT-29에 새로운 CDCA 합성유도체인 HS-1200을처치하여 암세포의 증식에 미치는 영향과 아포토시스 유도 활성을 관찰하고 기전을 연구하고자 하였다. 대상 및 방법 : 지수증식기의 HT-29 세포에 다양한 농도의 CDCA 합성유도체인 HS-1200을 투약하여 IC₅₀를 구하였다. IC₅₀의 농도를 참조하여, 세포 생존능의 실험은 trypan blue exclusion assay를 이용하였고, 아포토시스 유도에 관한 관찰 실험은 agarose gel electrophoresis, TUNEL assay 및 Hoechst staining을 이용하였다. Westerm blotting을 통한 PARP [poly (ADP-ribose) polymerase], caspase-3 및 DFF (DNA fragmentation factor)의 degradation 및 cleavage 등을 관찰하였다. Immunofluorescent method를 통한 cytochrome c 방출 측정 및 미토콘드리아 막전위 측정을 실시하였다. 결과 : HS-1200은 agarose gel electrophoresis 실험에서 DNA ladder의 관찰, TUNEL assay 및 Hoechst staining에서 아포토시스 세포들이 대량으로 관찰되는 결과로 미루어 아포토시스에 의한 세포 사망을 유도하는 것으로 사료되었다. 아포토시스에 의한 세포사망을 검증하기 위한 Western blotting을 통한 PARP cleavage, caspase-3 및 DFF 발현 관찰에서 공히 HS-1200 처치후 4시간 째부터 PARP, caspase-3 및 DFF의 degradation 및 cleavage가 관찰되었다. HS-1200의 아포토시스 유도에 이르는 기전연구에서 미토콘드리아의 역할에 주목하고 시행한 cytochrome c 방출 측정 및 미토콘드리아막 전위 (ΔΨm) 측정에서 공히 cytochrome c 방출 및 미토콘드리아막 전위 (ΔΨm)의 감소가 관찰되었다. 결론 : 인체 대장암 세포주(HT-29)에서 CDCA 합성유도체인 HS-1200을 이용한 세포 증식억제 및 세포 사망 기전 연구에서, 아포토시스의 유도가 HS-1200의 세포 사망 기전에 중요한 역할을 하는 것을 확인하였다. 이러한 아포토시스의 유도에는 미토콘드리아의 역할이 중요하게 관련되는 것으로 관찰되었다. 상기 결과를 토대로 HS-1200의 항암 치료제로서의 역할에 관한 기초 자료로서 유용성을 제시할 수 있었다. Purpose : To investigate the growth inhibitory effects, and the underlying mechanism of human colon cancer cell (HT-29) death, induced by a new synthetic bile acid derivative (HS-1200). Materials and Methods : Human colon cancer cells (HT-29) in exponential growth phase, were treated with various concentrations of a new synthetic bile acid derivative (HS-1200). The growth inhibitory effects on HT-29 cells were examined using a trypan blue exclusion assay. The extent of apoptosis was deter-mined using agarose gel electrophoresis, TUNEL assays and Hoechst staining. The apoptotic cell death was also confirmed by Western blotting of PARP, caspase-3 and DNA fragmentation factor (DFF) anal-ysis. To investigate the involvement of mitochondria, we employed immunofluorescent staining of cyto-chrome c and mitochondrial membrane potential analyses. Results : The dose required for the half maximal inhibition (IC₅₀) of the HT-29 cell growth was 100~150 μM of HS-1200. Several changes, associated with the apoptosis of the HT-29 cells, were reveal by the agarose gel eletrophoresis, TUNEL assays and Hoechst staining, following their treatment with 100 μM of HS-1200. HS-1200 treatment also induced caspase-3, PARP and DFF degradations, and the western blotting showed the processed caspase-3 p20, PARP p85 and DFF p30 and p11 cleaved products. Mito-chondrial events were also demonstrated. The cytochrome c staining indicated that cytochrome c had been released from the mitochondria in the HS-1200 treated cells. The mitochondrial membrane potential (ΔΨm) was also prominently decreased in the HS-1200 treated cells. Conclusion : These findings suggest that the HS-1200 - induced apoptosis of human colon cancer cell (HT-29) is mediated via caspase and mitochondrial pathways.

신형 ORB-Scan system과 Ultrasonic pachymetry에 의한 각막중심두께의 측정값의 비교

김대영,오신근,선경호,김동해 대한시과학회 2004 대한시과학회지 Vol.6 No.1

목 적 : ORB-ScanII (Version 3 .1 2) 와 DGH500 Ultrasonic pachymetry를 이용하 여 시력교정수술 전 각막중심두께를 측정하여 비교함으로써 수술의 안정성과 정확성 을 높이고자 하였다. 대상과 방법 : 본원에 시력교정수술을 받기 위해 내원한 환자 66 명 130안을 대상 으로 각막두께를 측정하였다. ORB-ScanII 은 어두운 조명에서 3 회를 측정하였고 DGH500 Ultrasonic pachymetry는 20 회 이상 측정하여 중심부의 가장 앓은 각막의 두께를 사용하였다. 두 측정장치의 평균과 표준편차를 알아보았고 일치성은 상관분석 과 회귀분석을 통해 알아보았다. 결 과 : 평균 각막중심두께는 ORB-Scan II z로 측정한 경우는 529 .45 :t 33.34μm 이 였고, DGH500 Ultrasonic pachymetry로 측정한 경우는 525.22 :t 3 1.21μm 이였으며, ORB-ScanII 이 DGH500 Ultrasonic pachymetry보다 평균 4.23 :t 7.91 따l 더 높게 측정 되었다. ORB-ScanII 와 DGH500 Ultrasonic pachymetry 의 상관계수는 0.972로 1 에 가까웠 으며 선형회귀분석을 통하여 회귀식을 구하였다. 결 론 : 두 기기로 측정한 값에서 가장 앓은 각막두께를 기준으로 함으로써 수술 의 안정성을 높였으며 수술 후에 발생할 수도 있는 각막 변형을 예방하는데 도움을 주였다. 하지만, 측정 방식에 따른 측정 장치의 오차를 줄일 수 있는 기술적 발전이 펼요하리라 생각된다. Purpose To investigate the accuracy of ORB-Scan system in measuring comeal thickness before refractive surgery, the authors conducted a comparative study of ORB• Scan system and ultrasonic pachymetry in their stability and accuracy. Methods : Central comeal thickness was rneasured by each meth여 in 130 eyes of 66 healthy subjects. The ORB-Scan measured 3 times from dark illumination and the DGH500 Ultrasonic pachymetry measured above 20 times center it used the thickness of the most thin comea. It examined the average and a standard deviation of two measuring equipments. Correlation and regression were analyzed to assess their agreement. Results The average central thickness was 529.45:1:33.34 .um by Orb-scan system, and 525.22:1:31.21 띠n by ultrasonic pachymetry. The ORB-Scan was more highly measured average 4.23:1:7.91 than DGH500 Ultrasonic pachymetry. Corre lation coefficient was nearly 1 between them, and therefore we obtained statis tically significant linear regression equation. Conclusion The value, thin thickness of the comea, measured by two machines raised the stability of an operation. Also, after operating it gives the help, which prevents the comea variation, which is the possibility of happening. But, the technical development which is the possibility of reducing the error of the measuring equipment which it follows in specific method is thought will be necessary.

우리나라에서 서식하고 있는 바퀴벌레의 분포에 관한 연구

권순완,오신근,윤운기,김성원,이용석,김광호,김명진,김정근,김대식,김효신,유인석,이수영,정병주,김규언,김동수,이기영,이한일 대한천식알레르기학회 1993 천식 및 알레르기 Vol.13 No.3

Studies on the infestation rate and population density of cockroaches have been investigated in Seoul, Pusan and Junbuk district for one month in May 1992. The sampling of the cockroaches in the study were conducted in the kitchens, bed- rooms, floors, and toilets in huses of 73 Allergic patients. Allergic patients who lived in Seoul, Pusan, and Junbuk district were selected. A sticky trap were used to attract cockroaches. The results are summerized as follows ' 1) The cockroaches were collected in 58 houses out of a total of 73 houses. This corresponds to a 78.1 percent infestation rate of cockroaches. The average number of cockroaches collected per house was 22.8. B. germanica was the most Common species, showing 81.2 percent of the collected cockroaches, followed by P.japonica(9.1%), P.fuligionsa(4.8% ), and P americana(4.5%). 2) P. japonica were collected almost uniformly in those three districts, but P. fuliginosa and P. americana were mainly collected in the Pusan district. 3) Houses in poor sanitation and those with an installed central heating system showed higher infestation rates. 4) The average number of cockroaches collected in patients houses whose residents sho- wed positive results to allergy skin test were 32.4. This number was significantly higher than that of cockroaches collected in the houses negative allergy skin test patients, where. 18.2 was the average number of cockroaches.

선천성 거대세포바이러스 감염증 2예

이혜진,오신근,안미수 대한감염학회 1993 감염 Vol.25 No.1

We have experienced two cases of Congenital cytomegalovirus Infection. The first case was a 50 days old female baby who was hospitalized in our hospital because of fever. She was born 37 weeks, 1880 gm. In the past history, she had received an exchange transfusion due to severe jaundice. She was characterized by microcephaly, failure to thrive, hepatosplenomegaly, petechiae, hemolytic anemia, thrombocytopenia and polymicrogyri on brain MRI. In her hospital days, she was improved, but after discharge, she expired because of status epilepticus. The second case was a 40 days old male baby who was hospitalized in our hospital because of marked abdominal distension. On his physical examination, he was characterized by microcephaly, hepatosplenomegaly, inguinal hernia and jaundice. He was improved and discharged. In both cases, CMV Ig M and Ig G was positive.

Importance of Balanced Attention Toward Coronavirus Disease 2019 and Neglected Tropical Diseases

용태순,김주영,오신근,윤문수 연세대학교의과대학 2023 Yonsei medical journal Vol.64 No.6

Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, has been spreading since 2019, causing a worldwide pandemic. Amid the COVID-19 pandemic, tuberculosis, AIDS, and malaria have adversely affected the quality of life of patients and killed mil lions of people. In addition, COVID-19 continues to impede the delivery of health services, including those for the control of ne glected tropical diseases (NTDs). Furthermore, NTDs have been reported as possible co-pathogens among patients infected with COVID-19. However, studies regarding parasitic co-infection among these patients have been limited. This review aimed to explore and describe the cases and reports of parasitic infections in the backdrop of COVID-19 to provide comprehensive knowledge re garding this aspect. We reviewed seven cases of patients who had parasitic co-infection and tested positive for COVID-19, and summarized the literature on the importance of controlling parasitic diseases. In addition, we identified recommendations for the control of parasitic diseases under possible difficulties, such as declining funding for parasitic diseases in 2020. This review high lights the growing burden of NTDs under COVID-19 that may be caused by the deficiency of healthcare infrastructure and human resources as the main reasons. Clinicians should remain vigilant for possible co-infections with parasites in COVID-19 patients, while policymakers are urged to reinforce a balanced and long-term health strategy that addresses both NTDs and COVID-19.

Intestinal parasitic infections among children aged 12–59 months in Nyamasheke District, Rwanda

Evariste Hakizimana,김주영,오신근,윤문수,용태순 대한기생충학ㆍ열대의학회 2023 The Korean Journal of Parasitology Vol.61 No.3

Intestinal parasitic infections are a public health burden and a major cause of illness in developing countries. The diseases lead to various health threats, including growth retardation and mental health-related disorders, especially in children. We assessed the risk factors for intestinal parasitic infections among children aged 12–59 months residing in Nyamasheke District, Rwanda. A cross-sectional descriptive study was conducted using secondary data from 1,048 children aged 12–59 months whose stool samples were examined for the presence of intestinal parasites and whose results were registered in the laboratory information system in 2020. The prevalence of intestinal parasites in children aged 12–59 months was 53.2%. The dominant parasites were Ascaris lumbricoides (13.1%), followed by Giardia lamblia (10.9%), Entamoeba histolytica (7.9%), Trichuris trichiura (6.5%), hookworms (1.7%), and Taenia species (1.4%). A significant association was observed between intestinal parasites and the literacy of mothers or children’s caregivers (odds ratio (OR)=5.09, P<0.001). Children from farming households were 2.8-fold more likely to contract intestinal parasitic infections than those from nonfarming households (OR=2.8, P<0.001). A significant association was also observed between intestinal parasites and food safety (OR=4.9, P<0.001). Intestinal parasitic infections were significantly associated with hand hygiene practices after using the toilet and washing fresh fruits before eating (P<0.001). The information gathered will help public health providers and partners develop control plans in highly endemic areas in Rwanda.