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간염, 간경변증 및 간암 조직내 림프구아형 분포에 관한 면역조직 화학적 연구
염범우 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.2
It is generally believed that the hepatic injury in viral hepatitis is due to cell mediated cytotoxicity. The natural Killer cells have defensive role on viral infection and on malignant neoplasm. In order to evaluate the role of natural killer cells in viral hepatitis, cirrhosis and hepatoma, the author performed immunohistologic study on hepatic lesions with MT1 for T Cells. LN2 for B cells and anti Leu-7 for NK cells. The results are as followings ; 1. In chronic active hepatitis, the natural killer cells are decreased in number compared with those in chronic persistent hepatitis 2. In liver cirrhosis natural killer cells are markedly decreased in number compared with those in chronic hepatitis. 3. In hepatoma, the natural killer cells are increased in unmber. Above results strongly indicated that the natural killer cells have defensive role in progress of viral hepatitis.
히스타민 및 항히스타민제가 L_(1210)백혈병세포계증식에 미치는 영향에 관한 실험적 연구
염범우,이대일,백승룡 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.1
There is close relationship between growth of tumor cell and immunologic function of the host. And growth of tumor cell also partially depends on histamine which indirectly modulate degree of immunologic response of the host. However, there is no report on direct effect of histamine on the growth of tumor cell. Therefore, to evaluate direct effect of histamine on growth of tumor cell, histamine and Hz-receptor antagonist (cimetidine) were added in Fischer's culture media in concentration of 10^(-3)M , 10^(-4)M and 10^(-5)M and cultured L1:10 leukemic cell for 24,72 and 96 hours. The results are as followings; 1. Histamine iii high concentration(10^(-3)M) inhibited the proliferation of L_(1210) cell lines. 2. H2-receptor antagonist showed no inhibitory activity on the proliferation of L_(1210) leukemic cell lines a t each concentration and (10^(-3), 10^(-4) and 10^(-5)). 3. The combination of histamine and H2-receptor antagonist showed temporary inhibitory effect on the L(1210) leukemic cell lines at 24 hours culture. Above results suggest that histamine show inhibitory activity to growth of L_(1210) leukemic cell lines through H2-receptor.
Lectin을 利用한 各種 組織固定液이 組織에 미치는 生化學的變化에 關한 硏究
廉範愚 고려대학교 의과대학 1988 고려대 의대 잡지 Vol.25 No.2
The fixative is an essential for tissue processing in surgical pathology. The best fixative is that it fixes the tissue rapidly and causes the least biochemical changes in-cellular level during fixation. But all fixatives may cause some biochemical changes during fixation. The lectin is plant extract having binding activity with specific carbohydrate terminals And so the lectins are widely used in order to find out the biochemical change in neoplastic cells, fetal tissue and mitotic cells and some lectins are used as cell markers. The author made an experimental study to dissolve whether fixative might change the biochemical composition of skin, liver, kidney and spleen using with 8 lectins by ABC method. The fixatives used are Boum's solution, neutral and acid formalin, ethyl alcohol and Zenker's solution. The results are followings; 1. The epidermis of skin fixed in alcohol revealed the strongest positive reaction to PNA and Zenker's solution to PTA. 2. The renal tubular epithelial cells fixed in ethyl alcohol revealed the strongest positive reaction to PNA, DBA, Con A, SBA and RCA-Ⅰ. 3 The lymphocyte of spleen fixed in alcohol and Boum's solution showed the positive reaction to PNA.
인체 태아 및 성인 세포에서의 c-myc, c-H-ras 및 EGF 항혈청에 대한 면역조직학적 연구
염범우 고려대학교 의과대학 1994 고려대 의대 잡지 Vol.31 No.2
The exact nature of carcinogenesis is still debatable, however the discovery of oncogenes and suppressor genes encouraged the frontiers to explain the pathogenesis of malignant change. In general, the oncogenes and suppressor genes are believed to involve in cellular transformation but now it is also believed that these genes also have the role of normal cell proliferation and differentiation. So the author performed this experimental study to find out the presence of c-myc, c-H-ras and EGF protein in the liver, stomach and kidney of fetuses of variable gestational age, new born infants and adults. The results are as follows: 1. c-myc antisera revealed highest positive ratio in the liver of fetuses and newborn infant. 2. c-H-ras antisera revealed highest positive ratio in the stomach of fetuses and newborn infant. 3. EGF antisera revealed positive ratio in the liver and stomach in 9 and 5 cases.
염범우,이우정 고려대학교 의과대학 1996 고려대 의대 잡지 Vol.33 No.1
It has been well known that carcinogenesis begins from alteration of specific genes, such as oncogenes and suppressor genes. Although the precise mechanism is still not known, it is generally accepted that oncogenes and suppressor genes are involved not only in carcinogenesis but also in cellular differentiation and proliferation. It is important to know oncogene found in tumors is limited to the oncogenes already presented in fetal tissues. So the author carried out immunohistochemical study with c-neu, c-fos and c-jun antisera on the DDY mouse fetal tissue of 12, 14, 16 and 18 old gestational days. The results are as fol- lows. 1. c-fos protein was present in 16, 18 days old fetal immature cartilage and intestinal mucosa. 2. c-jun protein was noted only on fetal immature cartilage of 16, 18days old fetus. 3. c-neu protein was noted on the fetal liver, intestine, glial tissue skeletal muscle and pancreas after 16days old fetus.
호지킨병의 Reed-Sternberg세포의 면역조직학적 연구
김창범,염범우,최종상 고려대학교 의과대학 1990 고려대 의대 잡지 Vol.27 No.3
Hodgkin's disease is a complex disorder in cellular and humoral immunity. The Reed-Sternberg cells are generally accepted to represent malignant cells in Hodgkin's disease, and numerous immunologic and electron microscopic studies raised the possibility of these cells to be originated from the B cell, T cell, marcrophage, reticulum cell and granulocyte. Now it is generally believed that lymphocyte predominance type of Hodgkin's disease is originated from germinal center cells, However, the origin of other types of Hodgkin's disease is unclear. The author studied Reed-Sternberg cells in 16 cases of Hodgkin's disease by immunohistologic method with MT1 of T cell marker, LN2 of B cell marker, antiLeu-7 of natural killer cell marker and antilysozyme of macrophage marker to clarify the origin of the cell. The results are as followings: 1. LN2 positive Reed-Sternberg cells were present in 15 of 16 Hodgkin's disease and the strongest reaction was noted in lymphocyte predominance type. 2. Antilysozyme positive Reed-Sternberg cells were present in 6 of 16 cases. MT1 and antiLeu-7 revealed no positive cells. 3. AntiLeu-7 positive lymphocytes were noted in 10 cases and especially in lymphocyte predominance type, in which the positive cells are markedly increased in number comparing with another types.
유방암과 액와부 림프절 전이암의 자연살세포 분포에 관한 면역조직화학적 연구
정미라,염범우,최종상 고려대학교 의과대학 1990 고려대 의대 잡지 Vol.27 No.1
Natural killer(NK) cells are cytotoxic lymphocytes mediating defence mechanism against to neoplasm, viral infection and some microorganism. Recently, NK cells are the focus of intensive research of their roles in malignant tumor and tumor metastasis. However, the mechanism of activation, regulation and killing of tumor cells remains unresolved. The author performed immunohistochemical study with anti Leu-7 on various benign and malignant breast tumors and regional lymph nodes to investigate the distribution of NK cells. The results are as followings. 1. NK cell in malignant breast tumor is present on the margin of tumor cell nests. 2. On benign lesion, NK cell is noted especially in fibrotic lesion. 3. In metastatic lymph nodes, NK cells are characteristically distributed in germinal center contrary to peripheral location in normal lymph nodes.