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연선희,권오상,조성진,강석윤,문지영,류연희 한국한의학연구원 2016 Integrative Medicine Research Vol.5 No.1
Background The novel threadlike structure is called the primo structure, and studies are conducted through many different approaches. Although various ways of differentiation are currently used, a standard for differentiation is deemed necessary in order to identify the primo structure based on the overall form of the structure. This study aims to explore the differentiation standard through the histological common point of the threadlike structure of rat and swine by using the hematoxylin and eosin (H&E) staining method commonly used for histological research in various structures. Methods An 8-week old Sprague-Dawley rat and a Yorkshire pig weighing 30–40 kg were used. A total of 65 pieces of rat threadlike structure and 100 pieces of swine threadlike structure were collected after the abdomen was cut and opened. The following three different characteristics were confirmed using the H&E staining method for the collected structures: (1) bright cell availability; (2) cavity availability; and (3) nuclei density. Results For the rat threadlike structure, the bright cell (70.5%) and nuclei density (92.6%) were mainly observed; in the swine threadlike structure, however, the bright cell (60.6%) and cavity (67.2%) were mainly observed. The bright cell was confirmed to have been observed in the threadlike structures of both rat and swine. Conclusion The bright cell is determined to be the common point in the primo structure. However, further research is deemed necessary in the future as to the functions performed by the characteristic shown by the Primo structure.
Falciform ligament(간겸상인대)에서 관찰되는 프리모 조직의 조직학적 특성 연구
연선희,권오상,이새봄,조성진,최광호,이상훈,최선미,류연희 한국한의학연구원 2012 한국한의학연구원논문집 Vol.18 No.2
Objectives : Primo vascular system is known to new circulatory system and suggested as a anatomical structure of meridian system. Primo-vessels are present throughout the whole body. The purpose of this study is to identify primo tissues taken from falciform ligament and to compare with organ surface primo tissue, blood vessel and lymph vessel. Methods : Male Sprague-Dawley rats (8weeks old, 250~320g) used for this study. The medial line of the abdomen was dissected and searched for primo tissues in falciform ligament and on the internal organs using stereomicroscope. We performed serial cross section and histological investigations. The tissues were stained with hematoxylin-eosin and Masson’s trichrome. Results : 1. The primo tissues attached on the falciform ligament had white color and length of 5~8mm. 2. We could observe primo tissues are classified with ligament tissues. 3. Histogogically, primo tissue on falciform ligament and organ surface primo tissue could be considered same tissue. Conclusions : In this study, we observed primo tissue discovered on the falciform ligament. And we also histologically compared such discovered primo tissue with organ surface primo tissue. Consequently, we could consider that two tissues have histological similarity and possibility of connection in one network system.
Histological similarity of primo vascular systems derived from three internal organs of rats
연선희,권오상,조성진,최광호,최선미,류연희 한국한의학연구원 2013 Integrative Medicine Research Vol.2 No.3
Background: The primo vascular system has been suggested as the third circulatory system. Although primo tissues have been observed in various tissues isolated from a number of animal species, including mice, rats, and rabbits, very few reports on the histological characteristics of primo tissues from different body parts are available. This study was designed to compare the histological characteristics of primo tissues isolated from different body parts of rats. Methods: Rats were anesthetized and operated on, to locate primo tissues. The primo tissues were searched at the all organs’ surface and in lymphatic vessels using suitable finding methods. The tissues found were then separated and observed by histological test methods. Results: This histological study revealed that there was no difference between the histological characteristics of the organ-surface primo tissues, the primo tissues inside lymphatic vessels, and the primo tissues on the falciform ligament. Moreover, primo tissues could be differentiated from those with a similar structure, such as lymphatic vessels and blood vessels, by immunostaining against a-LYVE-1 and a-CD31, and can be observed by specific immunostaining against the Von Willebrand factor (vWF). Conclusion: The results of this study support the fact that primo tissues could possibly constitute a third circulatory system in the whole-body network.
연선희,이새봄,권오상,조성진,최광호,이상훈,최선미,류연희 한국한의학연구원 2012 한국한의학연구원논문집 Vol.18 No.3
Objectives : Fire needling has been applied as the treatment for various diseases and been getting much attention from Oriental medicine due to its excellent effectiveness as the results of clinical studies have reported. However, the research findings on the safety of treatment method, materials for the Fire needling needle materials and the possibility of burn injury during the procedure are still insufficient. Methods : A thermo imaging camera was used to confirm the temperature distribution on acupuncture needle and the treatment area during the fire needling therapy. Then the degree of thermal injury was observed by H&E stain and TUNEL assay. In addition, in order to assess the safety of acupuncture materials, we conducted MTT assay using a L6 cell line. Results : The average temperature of the skin surface was observed at 47~51°C after classic fire needling and 30°C after warming fire needling. Warming fire needling therapy does not induce a burn on the tissue and a third degree burn was observed locally in the muscle and skin layers after classic fire needling treatment. This confirms that hwa-acupuncture therapies do not cause major burns. According to the safety assessment test result, no cytotoxicity was detected in the warming fire needling materials. This confirms the safety of the acupuncture materials Conclusions : Various research results on the biological safety of fire needling. Since fire needling therapy induces a burn locally without leaving any scar, and as other results indicate, it is considered a safe treatment method.
Interleukin-10 발현 B 세포가 만성 비부비동염의 병태생리에 미치는 영향
박희성,박수경,연선희,Jun Xu,나기상,김용민 대한이비인후과학회 2018 대한이비인후과학회지 두경부외과학 Vol.61 No.8
Background and Objectives Many kinds of inflammatory cells and cytokines are suggestedto be related with pathophysiology of chronic rhinosinusitis with nasal polyp (CRSwNP),but its mechanism is not yet fully understood. In particular, little is known about the functionand role of Interleukin-10 (IL-10) producing regulatory B cells. The aim of this study was toinvestigate the presence and function of B cells, especially IL-10-producing regulatory Bcells, in the nasal polyp (NP) and nasal mucosa. Subjects and Method Twenty patients with CRSwNP, 10 patients with chronic rhinosinusitiswithout nasal polyp (CRSsNP) and 10 control subjects were enrolled in this study. NP tissuesand uncinate tissues were collected for analysis. Real-time polymerase chain reaction (RTPCR),immunohistochemistry were performed to measure the expression levels of the selectedinflammatory cytokines and inflammation-associated molecules. Results In the mucosal tissues of CRSsNP patients, the number of IL-10+ B cells was significantlylower than that of NP and control mucosa of CRSwNP patients. The number of IL-10+B cells was significantly increased in the eosinophilic NP, non-eosinohilic NP, CRSsNP, andcontrol groups. There was a significant positive correlation between the number of IL-10+ Bcells and B cell activating factor (BAFF). Conclusion The expression of IL-10+ B cells and BAFF was significantly increased inCRSwNP patients compared to CRSsNP patients and control mucosal tissues, and IL-10+ Bcells were more significantly increased in eosinophilic tissues. These results indicate that regulatoryB cells are involved in the pathophysiology of eosinophilic NP and that BAFF contributesto the production of regulatory B cells.
박수경,한예녕,허준,연선희,이성복,송근호,변진희,나기상,김용민 대한이비인후과학회 2020 대한이비인후과학회지 두경부외과학 Vol.63 No.5
Background and Objectives The nucleotide-binding oligomerization domain-like receptor(NLRP) 3 is known as a member of the NLR family, and it has been confirmed that the NLRP3inflammasome is associated with various diseases such as asthma, inflammatory bowel disease,metabolic disorders and multiple sclerosis, as well as other auto-immune and auto-inflammatorydiseases. However, the role of NLRP3 in chronic rhinosinusitis with nasal polyps (CRSwNP)has not yet been explored. Subjects and Method Forty-four specimens of nasal polyps and 25 specimens of uncinateprocesses were collected from patients with chronic rhinosinusitis with nasal polyps, and 25specimens of uncinate tissues were collected from patients who underwent other rhino-surgeries. The western blot assay was employed to analyze the expression of NLRP3; interleukin (IL)-1βand IL-17A were detected using immunohistochemistry and real-time polymerase chain reaction. The production of lipopolysaccharide (LPS) induced IL-1β and IL-17A with or without theNLRP3 inflammasome inhibitor (MCC950) was measured using an enzyme linked immunosorbentassay in cultured dispersed nasal polyp cells. Results NLRP3 showed a high level of expression in nasal polyps than in the control group(p<0.01). The expression of IL-1β and IL-17A was significantly higher in nasal polyps in theCRSwNP group than in the control group (p<0.05). LPS-induced production of IL-1β was significantlysuppressed by treatment with the NLRP3 inflammasome inhibitor (p<0.05). Conclusion The NLRP3 inflammasome plays an essential role in the pathogenesis of CRSwNP,and thus MCC950 can be considered a prospective therapeutic for NLRP3 inflammasome-mediatedinflammation in nasal polyps. Our data provide new evidence that IL-17A is involved in inflammasome-associated inflammation in nasal polyps.