Integrated analysis of miRNA and mRNA reveals that acrolein modulates GPI anchor biosynthesis in human primary endothelial cells

양하나,이승은,이상곤,조정제,안현종,박증석,박용식 한국바이오칩학회 2013 BioChip Journal Vol.7 No.1

Acrolein (ACR) is a highly reactive α, β- unsaturated carbonyl compound that is related to several diabetic vascular complications. ACR, a ubiquitous aldehyde pollutant, is a product of incomplete combustion reactions and a major component of cigarette smoke. ACR is also endogenously generated during lipid peroxidation. Glycosylphosphatidylinositol (GPI)-anchored proteins is a membrane bound protein, which have a variety of cellular functions. It is reported that some of them showed the reduced level under diabetic conditions. In this study, we explored whether ACR may affect GPI anchor related gene expression in human umbilical vein endothelial cells (HUVECs) by modulation of miRNA expression that play a significant role in the post-transcriptional regulation of genes. We performed global gene expression analysis, acquired altered expression levels of 27 miRNAs and 21 mRNAs that are relevant to GPI anchored proteins, and observed their anti-correlations. Gene Ontology (GO) terms that are closely associated with GPI anchor biosynthesis were highest when analysis was based on biological processes characterized by enriched GO categories. Our results revealed that ACR may control miRNAs to regulate genes involving in GPI anchor biosynthesis in human endothelial cells.

Expression profile analysis of human umbilical vein endothelial cells treated with salvianolic acid B from Salvia miltiorrhiza

양하나,이승은,Dong Sun Ryu,Cheung-Seog Park,Young-Ho Jin,박용식 한국바이오칩학회 2011 BioChip Journal Vol.5 No.1

Salvianolic acid B (SalB) is a bioactive component in Salvia miltiorrhiza, which is widely used as a traditional oriental medicine for treatment of cardiovascular disorders. As it is known to have cardioprotective effects and anti-inflammatory activities, it has been used for treatment of several vascular diseases. However, the precise mechanism of SalB at the transcriptional level has not so far been determined. In this study, we investigated target genes of SalB in human umbilical vein endothelial cells (HUVECs) by microarray gene expression profiling that allows a global view of gene expression. Among more than 40,000 genes investigated, 140 genes were up-regulat-ed more than 1.7-fold, while 167 genes were down-regulated with altered expression levels of 2-fold. According to their functional characteristics, genes were classified into seven categories. We also showed the distribution of functional groups of target genes in SalB-treated HUVECs. Furthermore, cardiovascular disease-related genes, including PDGS2, TNFSF12, and IFNG, were also altered by SalB. These results suggest that SalB may exert a vasculoprotective effect through transcriptional change of inflammatory genes. In conclusion, our data suggest that these changes in gene expression mediate the anti-inflammatory activities of SalB on vasculopathy.

초등학생의 ADHD 하위요인 증상이 인터넷 과다사용과 집단따돌림 가해행동에 미치는 영향: 집단따돌림 피해경험과 스트레스의 매개효과

양하나,이동훈 한국교육학회 2018 敎育學硏究 Vol.56 No.4

This study was to investigate the mediating effects of victimization experience and stress on the relation between ADHD subtype symptoms and Internet overuse/bullying behavior. For this study, self-reports data were used from 774 students of elementary schools’ high grades for ADHD subtype symptoms, Internet overuse, bullying behavior, victimization experience, stress. Correlation analysis and Structural Equation Modeling(SEM) were employed to examine their relations. The result indicated that there was a double-mediating effect of victimization experience and stress between Inattentive of ADHD and bullying behavior. Also victimization experience was significant between Inattentive of ADHD and Internet overuse. However, Hyperactivity/Impulsivity of ADHD did not predict any variations. Based on the results, findings and implications were discussed. 본 연구는 초등학생의 ADHD 증상의 두 하위요인과 인터넷 과다사용 및 집단따돌림 가해행동 간 관계에서 집단따돌림 피해경험과 스트레스가 매개하는지 살펴보았다. B 지역에 소재한 20개 초등학교 4, 5, 6학년 40개 학급 학생들을 대상으로 ADHD 증상, 인터넷 과다사용, 집단따돌림 가해행동, 집단따돌림 피해경험, 스트레스 척도를 사용하여 설문조사를 실시하였고, 총 774명의 데이터가 분석에 사용되었다. ADHD 증상과 인터넷 과다사용, 집단따돌림 가해행동, 집단따돌림 피해경험, 스트레스 간 관계를 알아보기 위해 상관분석을 실시하였고, 집단따돌림 피해경험과 스트레스가 매개하는지 알아보기 위해 구조방정식을 사용하여 매개모형 검증을 실시하였다. 그 결과, ADHD의 하위유형 중 주의력결핍과 집단따돌림 가해행동 간 관계에서 집단따돌림 피해경험과 스트레스의 이중매개효과, 주의력결핍과 인터넷 과다사용 간 관계에서 집단따돌림 피해경험의 매개효과가 유의하였다. ADHD 하위유형 중 과잉행동‧충동성은 어떤 변인도 유의하게 예측하지 못하였다. 연구결과에 기초하여 논의 및 함의가 제시되었다.

Effect of Korean Red Ginseng treatment on the gene expression profile of diabetic rat retina

양하나,손건우,박혜림,이승은,박용식 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.1

Background: Korean Red Ginseng (KRG) is a herbal medicine used in Asian countries and is very popular for its beneficial biological properties. Diabetes mellitus (DM) and its complications are rapidly becoming a global public health concern. The literature on transcriptional changes induced by KRG in rat models of diabetic retinopathy is limited. Considering these facts, we designed this study to determine whether retinopathy-associated genes are altered in retinas of rats with DM and whether the induced changes are reversed by KRG. Methods: Male SpragueeDawley rats were intravenously injected with streptozotocin (50 mg/kg body weight) to induce DM, following which, KRG powder (200 mg/kg body weight) was orally administered to the KRG-treated DM rat group for 10 wks. The rats were then sacrificed, and their retinas were harvested for total RNA extraction. Microarray gene expression profiling was performed on the extracted RNA samples. Results: From among > 31,000 genes investigated, the expression of 268 genes was observed to be upregulated and that of 58 genes was downregulated, with twofold altered expression levels in the DM group compared with those in the control group. Moreover, 39 genes were upregulated more than twofold and 84 genes were downregulated in the KRG-treated group compared to the DM group. The expression of the genes was significantly reversed by KRG treatment; some of these genes were analyzed further to verify the results of the microarray experiments. Conclusion: Taken together, our data suggest that reversed changes in the gene expression may mediate alleviating activities of KRG in rats with diabetic retinopathy.

Differentially-expressed genes associated with glycophosphatidylinositol(GPI)-anchored proteins by diabetes-related toxic substances in human endothelial cells

양하나,이승은,정성일,박증석,진영호,박용식 한국바이오칩학회 2012 BioChip Journal Vol.6 No.3

Glycophosphatidylinositol(GPI)-anchored proteins are one of the membrane-bound proteins that have diverse functions. There have been some reports that their expression or activities are altered in diabetes. Many proteins, including GPI-anchored proteins, lose their biological function secondary to carbonylation in diabetes. Carbonylation causes oxidative damage of numerous proteins in the human body. Diabetic complications are related to carbonyl adducts in vascular tissue. Therefore, we examined whether car-bonyl compounds associated with diabetes affect the expression of GPI-related genes in human umbilical vein endothelial cells(HUVECs). Among the more than 150 GPI anchor-related genes investigated, 54 genes were up-regulated by more than 2-fold, while 31 genes were down-regulated with altered expression levels of 2-fold in acrolein(ACR)-treated cells. The majority of the genes changed by ACR involved GPI anchor biosynthesis. Crotonaldehyde and methylgly-oxal altered a few genes encoding GPI-anchored proteins. According to their functional characteristics, genes were classified into the Gene Ontology functional categories. We also identified the distribution of functional groups of GPI anchor-related genes in HUVECs. In conclusion, our data suggest that these reactive carbonyl compounds modulate GPI anchor-related gene expression, which may have a role in diabetic vasculopathy

Comparative mRNA and microRNA expression profiling of methylglyoxal-exposed human endothelial cells

양하나,김근동,박혜림,박용식 한국바이오칩학회 2013 BioChip Journal Vol.7 No.2

Methylglyoxal (MG) is a highly toxic compound that contributes to the formation of advanced glycation end products (AGEs). MG is detected at high concentration in plasma of diabetic patients and is involved in the pathogenesis of several diabetic macro- and microvascular complications, such as atherosclerosis,retinopathy, nephropathy and hypertension. MicroRNAs (miRNAs) play a critical role in the negative regulation of genes in many biologic processes. They are also known to post-transcriptionally regulate gene expression involved in cellular responses to toxicants. In this study, we investigated whether miRNAs play a role in the regulation of gene expressions in human umbilical vein endothelial cells (HUVECs) after the treatment with MG. We performed pair-wise correlation analysis, acquired altered expression levels of 274 miRNAs and 686 mRNA, and observed their anti-correlations. Genes associated with diabetic vascular disease were sorted from miRNAcorrelated genes, and Gene Ontology (GO) enrichment analysis on the differential expression of selected genes was carried out. Our results revealed the relationship between miRNAs and mRNA in MGexposed endothelial cells using the expression profiling.

Aloe-induced Toxic Hepatitis

양하나,김동준,김영묵,김병호,손경민,최명진,최영희 대한의학회 2010 Journal of Korean medical science Vol.25 No.3

Aloe has been widely used in phytomedicine. Phytomedicine describes aloe as a herb which has anti-inflammatory, anti-proliferative, anti-aging effects. In recent years several cases of aloe-induced hepatotoxicity were reported. But its pharmacokinetics and toxicity are poorly described in the literature. Here we report three cases with aloe-induced toxic hepatitis. A 57-yr-old woman, a 62-yr-old woman and a 55-yr-old woman were admitted to the hospital for acute hepatitis. They had taken aloe preparation for months. Their clinical manifestation, laboratory findings and histologic findings met diagnostic criteria (RUCAM scale) of toxic hepatitis. Upon discontinuation of the oral aloe preparations, liver enzymes returned to normal level. Aloe should be considered as a causative agent in hepatotoxicity.

고구마를 첨가한 저맥아 맥주의 양조와 품질 특성

양하나 ( Ha-na Yang ),오은비 ( Eun-bi Oh ),박정섭 ( Jeong-seob Park ),정문웅 ( Mun-yhung Jung ),최동성 ( Dong-seong Choi ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.3

신율미와 신자미 페이스트, 맥아, 효소제, 홉을 사용하여 고구마-맥아 당화액을 제조하고, 하면 발효효모 및 상면 발효효모를 접종, 발효하여 라거 및 에일 고구마 맥주를 제조하였다. 고구마 당화시 α-amylase 계 효소제를 첨가했을 때 여과성 향상과 당도 증가 효과가 가장 좋았다. α-Amylase 계 효소제를 0.1% 첨가하여 three step infusion 법으로 고구마-맥아당화액을 제조했을 때 맥주 양조를 위한 최적 당도인 13.5°Brix를 갖는 당화액이 얻어졌다. 라거 및 에일 신자미 맥주에서 고구마 첨가량이 증가함에 따라 폴리페놀 및 안토시아닌 함량이 증가하였고, DPPH 및 ABTS 라디칼 소거효과도 크게 증가하였다. 신율미 맥주에서는 감소하는 경향을 나타내었다. 고구마 맥주의 항산화 활성과 폴리페놀 함량 사이에는 강한 상관관계가 있었다. 라거 및 에일 신자미 맥주 모두에서 고구마 41.6%를 첨가했을 때 관능평가가 양호하였고, 미려한 적색의 색조를 띄었다. Sweet potato-malt worts were prepared by using sweet potato paste of Shinyulmi and Shinjami as the main adjunct, enzymes, malt, hop, and water. We brewed low-malt beers of the lager- or ale-type by using these worts and inoculating bottom and top fermenting yeast, respectively. Moreover, the componential and functional characteristics of the resulting beers were evaluated. During saccharification of sweet potato, the addition of an enzyme agent containing α-amylase caused an improvement in filterability and an increase of total sugar. The sugar content of sweet potato-malt wort which was prepared by the addition of 0.1% enzyme agent containing α-amylase and a three-step infusion procedure was 13.5 °Brix adequate for beer brewing. The polyphenol and anthocyanin contents of Shinjami beer were increased with increasing content of the paste, and also increased DPPH and ABTS radical scavenging activities. But in Shinyulmi beer it were decreased. A strong correlation was obserbed between antioxidave activities and polyphenol and anthocyanin contents of sweet potato beers. In all lager- and ale-type low-malt beers using 41.6% of Shinjami pastes, sensory attributes very similar to those of 100% malt beer were obtained and they were very good as they had unique red color.

모바일 환경에서 사용자 중심의 정보 Re-design

양하나(Ha-na Yang),김현수(Hyun-Soo kim),김찬일(Chan-il Kim) 한국HCI학회 2014 한국HCI학회 학술대회 Vol.2014 No.12

AHP 기법을 활용한 수강신청 및 진로선택 지원 시스템 개발 및 효과 분석

주하나,민상원,양영식,이효석 대한산업공학회 2009 대한산업공학회 추계학술대회논문집 Vol.2009 No.10