Lorazepam 제제의 용출에 관한 연구

양재헌 한국약제학회 1984 Journal of Pharmaceutical Investigation Vol.14 No.4

The tests of dissolution and bioavailability of six formulas of lorazepam tablet under various binders were performed. Lorazepam can be seperated in pharmaceutical preparation and biological plasma by high performance liquid chromatograph. Dissolution process of lorazepam tablet was largely influenced by binder and the dissolution rate was increased by sodium starch glycolate and microcrystalline cellulose, the slower dissolution rate in starch. Bioavailability of lorazepam tablet got relationship with dissolution rate, because tablets containing sodium starch glycolate and microcrystalline cellulose as binder maintained higher plasma level than other binders.

마우스의 면역반응에 미치는 백모등엑스의 효과

梁在憲,殷在淳,韓相贊 又石大學校 1995 論文集 Vol.17 No.-

Solanum lyratum(SL)has been used as a herbal drugs for an anticancer, antiinflammatory, antitoxic agents, ect. The present study was to carried out to investigate the several immune-modulatory effects of Solanum lyratum extract on histamine biosynthesis, lymphocyte blastogenesis, and hemolytic plaque forming cell assay against to in C57BL/6 mice. Histamine content lymphocyte blastogenesis and hemolytic plaque forming cell were deter mined by enzyme-isotope method, (^3H)Thymidine incorporation and cunningham method, respectively. Cytotoxicity, which was showed as 50% mortality was occurred by the treatment of SL(10^-3g/ml, 48hr incubation). Histamine production was significantly increased by the addition of 1.0㎍/ml of Con A after 48hr incubatin. SL suppressed histamine production at all concentration of SL(10^-9∼10^-3g/ml). Con A(0.1㎍/ml)dependent T-lymphocyte proliferation was significantly increased by the addition of 10^-7g/ml of SL. And antibody formation markedly increased at 5×10^-4g/㎏. This results suggested that SL have an enhancement action of the immune response, SL in creased cell mediated immune response and also enhanced antibody production(IgG, IgM).

白毛藤엑스의 抗炎作用에 관한 硏究

梁在憲,殷載淳,金喜 又石大學校 1994 論文集 Vol.16 No.-

These experiments were conducted to investigate the anti inflammatory and analgesic action of Solanum lyratum extract(SL). The results were summarized as follows : The inhibitory effect of the leakage of evans blue into peritoneal cavity was significantly increased to be 46.4%, The paper disk granuloma formation displayed more potent effect than the control group. Mouse pew edema induced by histamine was significantly decreased. SL(75㎎/pouch)inhibited the protein exudation and did not the leucocyte migration in rats. But SL(25㎎/pouch) inhibited the leucocyte migration. The analgesic activity on 0.7% acetic acid induced writhing syndrome was significantly shown.

기간의 정함이 있는 고용계약에 관한 연구

양재헌 경희대학교 산업관계연구소 2010 産硏論叢 Vol.35 No.-

Through the rules of employment contract which has a certain period, the problems presenting today is instability of irregular jobs. This thesis has a focus on solving irregular job's employment instability which has to transfer jobs every 2 years because of the period limitation of transition to regular job according to present law of irregular jobs. This problem in employment period has the point in solving burden of transferring irregular jobs to regular jobs to user relatively. So the main rule has a point to guarantee employment flexibility and solve the employment instability which workers have to wander around through employment cycle like revolving doorsvery 2 years. For example, replacing legal maxim unemployment. We have to substitute 3 years mentioned in the civil law with the minimum employment period and maximum employment period. And maximum employment period, according to rule No.662 it can be renewed silently in same condition, and if the management performance is stable, it can retain in the long run comparatively, it can satisfy both users and workers. But to apply a law to corporations which have more than 5 employees, this revised plan can be applied by overall revision of the law about No.16:1year limitation and or 2nd clause of Article4: short term or part time workers or the 1st clause of Article6:2year limitation from the Labor Standard Act which is more special than the common law: the civil law. 기간의 정함이 있는 고용계약의 경우 현실적으로 대두되고 있는 문제는 비정규직의 고용불안이다. 현행 비정규직법(기간제법 및 파견제법의 통칭)에 의거 2년이라는 정규직 전환의 기간제한 때문에 2년 마다 직장을 옮겨야 하는 회전문식 순환고용에 대한 비정규직의 고용불안을 해소하는 데 역점을 두었다. 이러한 고용기간의 문제는 상대적으로 사용자에게 비정규직을 정규직으로 전환하게 되는 부담을 해소하게 하는데 핵심이 있다. 따라서 사용자에게는 고용의 유연성을 보장하고, 숙련된 노동력을 안정적이고 지속적인 활용이 가능하도록 하는 한편, 노무자에게는 2년 마다 반복되는 회전문식 순환고용으로 이곳저곳으로 떠돌아야 하는 고용불안을 해소하도록 하는데 제안취지가 있다. 민법상 3년이라는 최장고용기간을 최단고용기간과 최장고용기간으로 대체한다. 그리고 최장고용기간이 경과한 후에는 제662조에 의거 동일한 조건으로 묵시의 갱신이 가능하도록 함으로써 경영실적이 안정적일 경우 비교적 장기간동안 고용계약을 존속시킬 수 있기 때문에 사용자와 노무자 모두의 이해관계를 충족시킬 수 있을 것으로 사료된다. 다만 5인 이상의 사업장에 확대 적용하기 위해서는 근로기준법 제16조의 1년 제한규정과 기간제 및 단시간근로자 보호 등에 관한 법률(이하 ‘기간제법’) 제4조 제2항 및 파견근로자보호 등에 관한 법률(이하 ‘파견제법’) 제6조의2 제1항1호의 2년 제한에 대한 규정을 폐지해야 한다.

가스크로마토그라피에 의한 에페드린 및 슈도에페드린의 동시 분리정량에 관한 연구

梁在憲,高華溶,金東洙 又石大學校 1993 論文集 Vol.15 No.-

The trifluoroacetyl (TFA) derivatives of the optical isomers of ephedrine (EP) and pseudoephedrine (PEP) were analysed by gas chromatography conditions (Column:3% OV-17, Temperature 120℃, detector : FID), showing retention time as EP (5.4 min), PEP (7.9min). The optical reaction conditions for acetylation of EP and PEP using TFA were temperature : 50∼80℃, time : 5-60min., the shortest time under N_2 gas for preventing its from evaporating and being injected into GC within 2 hrs. The recovery percentages of commercial domestic products were good results such as EP : 98.3±2.5%, PEP : 106.6±4.6% and the simultaneous determination of Ep and PEP were achieved. The contents of EP in commercial domestic tablets and syrups were EP : 93.4±0.1%-102.4±4.8%, PEP : 90.9±9.3-110.0±6.9% and EP : 109.4±2.2%, PEP : 106.3±1.1% respectively, in case of preparations in hospital pharmacy EP : 97.5±1.7-102.9±1.9%, PEP : 90.8±0.7-99.7±0.4%. From the upper results, this method is not only a rapid, precise and reproducible method in analysis ephedrine and pseudoephedrine in products but also is able to be used the quality control of a compound drugs. In advance it will be very useful for the pharmacological experiments in vivo.

醫藥品 製劑中 保存劑의 分離定量에 관한 硏究

梁在憲 又石大學校 1985 論文集 Vol.7 No.-

Preservatives are materials added to pharmaceutical formulations to protect the product from microbial contamination. A given preservative material can be used in a wide variety of products and also may be used in combinations with other preservatives. It is currently of interest with in the pharmaceutical industry to assure that specific, stability indicating, and validated testing methods are available for antimicrobial preservatives. This study reports the development of a revers phase high performance liquid chromatography (HPLC) that quantitate the several antimicrobial preservatives benzoic acid (sodium benzoate), methyl paraben, ethyl paraben, propyl paraben and butyl paraben in a series of typical pharmaceutical formulations. The proportions of methanol and water as well as the type and amounts of modifiers are varied in order to achieve optimum chromatography. This method has been used successfully to quantitate preservatives in solutions, syrups, suspensions and injections, and can be readily adapted to routine automated assays either for routine product evaluation.

Berberine 含有生藥의 β-cyclodextrin 포접화합물의 生體利用率에 관한 硏究

양재헌,박선자,김영일 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

To increase the bioavailability of berberine inclusion complex were prepared and studied in this experiment. Inclusion complexes of berberine(BER) and methanol extract of Coptis Rhizomia(MEX) with B-cyclodextrin were prepared. The solubilities of BER and MEX were 0.14% and 1.79%, while those of BECD and MECD were 0.36 and 3.22% respectively. The lipid-water partition coefficients of Ber-B-CD indlusion complex(BECD) and MEX-B-CD inclusion complex(MECD) were more increased than BER and MEX respectively. The absorption rates of BECD and MECD in stomach, duodenum, and jejunum of rats were compared with those of BER and MEX, which were more increased than BER and MEX respectively. The times required for the maximum serum concentration fo berberine from BER, MEX, BECD and MECD in mice were 30 minutes after oral administration. The maximum serum concentrations of berberine from BECD and MECD were higher than those from BER and MEX respectively. BECD was shown more increased antibacterial activity to all the gram positive bacteria, gram begative bacteria and mold than BER,MECD was shown more increased antibacterial activity to only S. epidermidis, K. pneumoniae, E. coli than MEX. The dissolutions of BECD and MECD were higher than BER and MEX respectiely. Therefore, it suggest that preparing of inclusion complex brings to prevention of coprecititated formation and improvement fo bioavailability of berberine.

Lorazepam製劑의 安定性에 관한 硏究

梁在憲,嚴東玉 又石大學校 1983 論文集 Vol.5 No.-

Lorazepam (7-chloro-5(0-chlorophenyl)-1.3-dihydro-3-hydroxy-2H-1.4-benzodiazepine-2-one) is a new minor tranquilizer of the benzodiazepine class. It is extensively used as a sedative and antianxiety agent in clinical practice. According to the pharmacology, Lozepam is superior to other benzodiazepines with respect to its anticonvulsant, muscle relaxing and antianxiety properties. The biological toxicity of Lorazepam is low and it's therapeutic index is high. For the research of Lorazepam preparation, we performed the tests of stability, high performance liquid chromatography, bioavailability, safety of five formulas of syrup and six formulas of injection of Lorazepam. Quantitative analysis of Lorazepam preparation is possible by spectrophotometric determination, but biological plasma level and impurity of thermolysis of Lozepam can be separated by using of high performance liquid chromatograph. To add saccharide in Lorazepam syrups is more effective for the prevention of thermolysis and sorbitol is more effective than other saccharides. Manufacturing Lorazepam injection, propyleneglycol is suitable to increase solubility of active ingredient and control of pH, and for stabilization of injection nicotinamide and PEG 1500 were more effective than other stabilizers. When Lorazepam syrup is orally administered to rabbit, it shows a high blood level after half an hour. Plasma level of Lorazepam is showed to be identical phase under various stabilizer when Lorazepam injection is intravenously administered to rat. It shows a substaining effect to add polyethylenglycol 400 and 1500 as stabilizer. PEG 1500 and Na_2S_2O_2 increased toxicity of Lorazepam injection, decreasing of LD_50 value in rat or mouse. Thiourea and nicotinamide have lower toxicity than other stabilizers in rate or mouse.

Norgestrel錠劑의 溶出에 관한 硏究

梁在憲 又石大學校 1984 論文集 Vol.6 No.-

Norgestrel is a potent progestrone type contraceptive and presently gaining wide acceptance in therapeutic practice. In all cases it is prescribed in such low dosage level as to necessitate the availability of sensitive techniques for its evaluation. In the last few years when the spectrophotometric and fluorometric determination of steroids have been accomplished, many papers have been published on the analysis and separation of Norgestrel. This paper describes the analysis of Nortestrel by HPLC and observe the dissolution profile of Norgestrel tablet under various binders. The analytical procedure outlined in this paper afford a specific, quick and accurate assay for Norgestrel by reverse phase high performance liquid chromatography, commonly used in oral contraceptive preparation. The dissolution rate of Norgestrel tablet was increased b sodium starch glycollate in distilled water and simulated gastric juice.

黃蓮解毒湯의 製劑化 및 品質管理에 관한 硏究

양재헌,박현구,김영일 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

A traditional prescription, Hwangryean-haedog-tang(HRHT) was composed of Coptidis Rhizoma, Phellodendri Cortex, Scutellariae Radix, Gardeniae Fructus(1.5:1.5:3.2) and it has been used in the treatment of hypertension. insomnia, mental disorder, medicinal poisoning, crapulence. The precipitation was formed during the preparation of decoction of HRHT. This precipitatiion was very slightly soluble in water. Because this precipitated reaction decrease the effect of a gerbal medicine, the content of berberine and baicalin was determined in the precipitated product and supernatant. Berberine which active ingredient of Coptidis Rhizoma, Phellodendri Cortex was identified and determined by HPLC using u-Bondapak C_18 column with acetonitrile:pH 5.2 phosphate buffer(60:40v/v). Baicalin which active ingredient of Scutellariae Radix was identified and determined by HPLC using u-Bondapak C_18 column with 0.5% phosphoric acid:acetonitrile(73:27v/v). The content of erberine in the coprecipitated product and supernatant were 11.6% and 1.5% and the content of baicalin were 20.7% and 2.4% respectively. To ingibit the precipitated reaction of HRHT. Coptidis Rhizoma and Phellodendri Cortex were mixed and extracted whth hot water, it were made into gastric-coated granule, Scutellariae Radix and Gardeniae Fructus mixed and extracted, it were made into enteric0coated granule.