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양영철 현대사진영상학회 2003 현대사진영상학회논문집 Vol.6 No.-
When Pusan International Film Festival started in 1996, Busan Film industry started too. We hope Busan to become the main city of Korean Film and Entertainment Industry. These days Busan Film Commission and the City Government are propelling several policies to develop it. So, I propose four things urgent to do. The first is that we need to make a long term-master plan and proper systems to carry it to effect. The second is to shape Busan Film Funds for many producers in Busan to make good movies. the third is that we have to think about not only movies but the whole Culture Contents area, the fourth is that all the wills and efforts of the people working for the Busan Movies should be put together.
간부분 절제술 후 비장내 이식한 간세포화 줄기세포의 분화에 관한 형태학적 연구
양영철,박재홍,박중규,배기원 한국생명과학회 2002 생명과학회지 Vol.12 No.3
This study was performed to evaluate regenerative effects of intrasplenic stem cell transplantation after partial hepatectomy. To evaluate the regenerative effects, Sprague Dawley rats were used. In vivo the embryonic stem cells of blastocysts were collected from superovulated rats on day 3.5 after the vaginal plug checked. The embryonic stem cells were cocultured with hepatocytes for 8 days, they were transplanted into the spleen. After the intrasplenic transplantation of cultured stem cells, they were initially distributed near the periarterial lymphatic sheath after transplantation in the hematoxylin-eosin staining. Their number were formely increased and their size enlarged at forming small lobules. The embryonic stem cells in the culture proliferated and initially proliferated around the periarterial lymphatic sheath and later they around the trabecula with blood vessels. After the transplantation of stem cells, their cell organelles were well developed rough endoplasmic reticulum at the 20th with prominent epidermal growth factor reaction, developed smooth endoplasmic reticulum at the 30th day, well differentiated bile canaliculi with increased transforming growth factor-$\beta$ and apoptosis reactions. 흰쥐에서 간부분 절제술 후 비장내 배자줄기세포를 이식하여 배자줄기세포가 간세포로 분화하여 장기간에 걸쳐서 비장내에서 간세포의 기능적인 구조를 유지하는지를 조사 하였으며 간세포로의 재생 효과에 미치는 영향을 조사하여 다음과 같은 결론을 얻었다. 비장내 세포이식에서 배자줄기 세포를 이식하면 처음에는 동맥주위림프초 근처에 위치하여 그 수가 먼저 증가하면 육주 주위에 소엽상으로 모여서 세포의 크기가 커졌다. 세포내 소기관의 발달은 이식 후20일에 가장 현저한 발달을 보이며 이때 EGF의 반응도 가장 현저하였다. 이식 후 40일이 되면 세포질내 소기관의 발달이 간세포의 기능이 가능할 정도로 분화되었으며 TGF 반응이나 apoptosis 반응은 증가하였다.