인태아 하요척관절의 관절원판 발육에 관한 연구

안호범(Ahn Ho Beom),윤재룡(Yoon Jae Rhyong) 대한체질인류학회 1993 대한체질인류학회지 Vol.6 No.1

하요적관절은 상요적관절과 같이 회내 회외운동을 하며 관절원판은 하요적관절과 요골수근관정 사이에 존재하고 있다 관절원편은 하요척관절의 안정을 유지시키는데 중요한 구조로서 섬유대 또는 섬유연골대가 적고 경상돌기에서 요골하단부내연을 연결하고 있다. 수근관절의 발생에 관해서는 양은 연구보고가 있으나 하요적관절 발육에 관한 보고는 드물고 관정원판의 선자현미경석 관찰은 없는 듯 하다. 저자는 좌고 30mm 부터 260mm 까지의 인태아를 대상으로 하요적관절과 관절원판의 발육과정을 광학 및 전자현미경적 관찰로 다응과 같은 결과를 얻었다. 좌고 40mm(태령 10주 ) 태아때 하요척관절과 요골수근관절의 원시강이 줄현하였고 100mm( 태 령 15주 ) 태아때는 관절강의 완성을 관찰하였다. 관설원판의 원기는 좌고 40mm 태아때 미분화 간엽세포의 밀집대로 줄현하였고 이세포에는 대량의 유리리보솜과 소량의 조연내형절망 사립제 및 골지복합제를 함유하고 있었다. 좌고 100mm( 태령 15 주 ) 태아때 관절원판의 외측부에는 방주상의 섬유모세포들이 치밀하게 배열하고 있었고 중간부에는 작은 원형의 섬유모세포들이 소생으로 배열하고 있었다. 좌고 150mm( 태령 19 주 ) 태아때 관절원판의 섬유모세포의 크기가 증대되었고 교년 섬유는 미량 존재하고 있였다. 좌고 200mm ( 대령23주) 부터 260mm (태령 30주) 태아까지의 관철원판 외즉부에는 섬유모세포와 다량의 교원섬유블이 출현하였고 중간부에는 섬유모세포와 교원섬유가 엉켜 존재하고 있었다. 이와같은 결과로 미루어 인태아 하요적관절의 관절원판은 태생기에 섬유모세포로 존재하고 생후에 섬유연골세표로 분화 발육하리라 생각된다.

개방창 감염에 대한 Flomoxef의 임상 효과

윤강모,안호범,김대영,이삼용,조백현,이민철 최신의학사 1995 最新醫學 Vol.38 No.5

융기성 피부섬유육종의 치험2례

이삼용,안호범,조백현,윤강모 大韓成形外科學會 1996 Archives of Plastic Surgery Vol.23 No.3

Dermatofibrosarcoma protuberans is an uncommon soft tissue neoplasm that usually presents as a painless, often long standing mass arising in the dermis of the skin. In most patients, the nodule enlarges slowly and painlessly for a period of 3 months to 2 years as it infiltrates the adjacent tissue and becomes multinodular. At the later stage, however, its growth rate accelerates, and it can ulcerate, bleed, or become painful. Histologically, on light microscopy, this tumor shows the characteristic cartwheel patterns, with the fibroblasts arranged radially about a small central hub of fibrous tissue, and on electron microscopy, fairly monotonous population of spindle-shaped cells with a few stellate cells. Many of the spindle cells contained intracytoplasmic aggregates of microfilaments with dense bodies and were partially surrounded by basal lamina, interpreted as being typical of myofibroblasts. Other cells showed typical features of fibroblasts Dermatofibrosarcoma protuberans typically arises on the trunk and proximal extremities. head and neck and scalp lesions have also been described. It is more frequent in men with a peak incidence during the third decade of life. We have encountered 2 cases, develpoed on the both ear lobules of a 25-year-old woman and the right lower of a 7-year-old child ; but there were no evidence of metastasis. A scapular fasciocutaneous free flap and a skin graft covered large defects resulting from wide tumor excision. Follow-up at 1 year showed the patient doing well with no evidence of local or regioal recurrence.

흰쥐 적출대동맥의 자외선 유발 기계적 반응시 cAMP 및 cGMP의 농도변화

류봉수,안호범,김대영,이삼용,조백현,백영홍 大韓成形外科學會 1994 Archives of Plastic Surgery Vol.21 No.4

When the isolated thoracic aortae of rats were contracted with 1 or 10 μM phenylephrine (PE), ultraviolet(UV) light irradiaion produced the contractile and relaxant and relaxant responses in preparations with and without endothelium, respectively, 1μM ACh relaxed PE-induced contraction in the intact endothelial preparation, but did not in the denuded one. On the other hand, nitroprusside (NP) and isoproterenol(ISP) produced relaxing resposes irrespective of the presence of the endothelium. In the Endothelium - intact preparation, ACh potentiated the UV light-induced contraction, but ISP did not. Both agents did not affect the UV light-induced relaxation in the denuded preparation. The cGMP level in tissue was decreased by removal of the endothelium, but the cAMP level was not. In both preparations with and without the endothelium combined treatment with NP 1μM after PE 10μM markeldly increased the cGMP level. However, combined treatment with PE 10μM + ACh 1μM increased cGMP level in the intact preparation only, and UV light irradiation for 3 min reduced the increment. Single treatment with PE 10μM and combined treatment with PE+ISP for 5min or PX+UV irradiation did not affect the cGMP level. And cAMP level of the intact endothelial preparation was increased in combined treatment with PE 10μM+ISP 1μM but not altered in any of the other conditions. These resluts suggest followings : ① UV light produces contraction of the rat thoracic aorta in endothelium-dependent fashoion, ② cAMP is not a factor to this response, ③ the UV light-induced relaxation is not related to the cGMP and the cAMP level in the tissue.

코뼈 골절의 전산화 단층 촬영에 의한 분류

노복균,안호범,김대영,이삼용,조백현 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.2

The reduction of nasal bone fracture has been done by a simple procedure. The nasal bone fracture was not a serious problem and patients are usually treated as outpatients. However it is causing increased demand for medical services far cosmetic reasons. We studied the nasal bone fracture by retrospective analysis, it comprising 606 patients with nasal bone fractures in various accidents and treated of Chonnam University Hospital from March 1995 to February 1998. We reviewed and analyzed the medical records and facial bone CT scans of 606 patients. The following results were obtained. 1. The prevalent age group was in the third decade, the most common cause was traffic accidents, while nasal swelling, tenderness, nasal deviation and crepitation were examined 2. The classification of nasal bone fracture was done by facial bone CT. Class 1: Nasal tip depressed fracture(16%) Class 2: Displaced nasal bone fracture without depression(30%) Class 3: Displaces nasal bone fracture with depression(12%) Class 4: Comminuted nasal bone fracture (10%) Class 5: Simple fracture of nasal bone and frontal process of maxilla(14%) Class 6: Comminuted fracture of nasal bone and frontal process of maxilla(11%) Class 7: Nasal bone fracture without displacement(9%)

인태아 수지굴근건의 발육에 관한 전자현미경적 연구

윤재룡,안호범,남광일,Yoon, Jae-Rhyong,Ahn, Ho-Beom,Nam, Kwang-Il 한국현미경학회 1996 Applied microscopy Vol.26 No.2

The development of flexor digital tendon of the hand was studied by electron microscopy in human fetuses ranging from 9 mm to 260 mm crown rump length. The primordium of tendons was first identified as discrete collection of mesenchymal cells at 25 mm fetus. Synovial sheath formation had commenced by 40 mm fetus and was complete by 70 mm fetus. Cell junction or adhesion sites at all ages were noted between the tendon cells. When dilatation of the synovial cavity occurred, two types of synovial cells were observed. A-type cells had numerous vesicles and large vacuoles. In contrast, B-type cells were characterized by abundant rough endoplasmic reticulum and well-developed Golgi complex. By $150mm{\sim}260mm$ fetuses, a mojority of the synovial cells were type B. The most remarkable difference between the synovial cells of full-term fetus and adult was the larger amount of collagen fibers in the latter. The vascular buds were first observed between the individual fibril bundles in the interfascicular space at 150 mm fetus. At 25 mm fetus, collagen fibrils were first noted within narrow cytoplasmic recesses which were continued with the extracellular space. Collagen fibrils were filled in almost entire extracellular space at 150 mm fetus. Besides collagen fibrils in the extracellular space small elastic fibers were also identified and followed in their development.

흉배혈관의 근피 천공분지에 기초한 유리피판의 임상적 이용

조규성,김대영,안호범,이삼용,조백현 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

The latissimus dorsi muscle or musculocutaneous flap is one of the most useful flaps in reconstructive surgery. This flap has many advantages, such as its reliable anatomy, long pedicle with large caliber vessels, minimal functional deficit of the donor site, and low incidence of donor site complications. However, the bulkiness of the flap has been considered a disadvantage, so various modifications of technique have been devised. The cutaneous portion of the flap can be safely elevated based on the cutaneous perforating branch of the thoracodorsal vessel. From March 1997 to February 1998, 10 patients underwent reconstructive procedures with thoracodorsal perforator-based free flaps. The composition of the flaps varied in accordance with the nature of the defect. The variances in the flaps were as follows; 3 were cutaneous, 6 musculocutaneous, and 1 osteomusculocutaneous including the rib. All flaps survived with good contour. We concluded that this thin and reliable flap was useful for reconstruction of various defects, and that the composition of the flap, such as subcutaneous, muscle and bone, gave it considerable flexibility as needed.

횡복직근피판을 이용한 치험례

김대영,이삼용,안호범,조백현,류봉수,윤강모 大韓成形外科學會 1995 Archives of Plastic Surgery Vol.22 No.2

Adequate soft tissue coverage is a primary requisite for the reconstruction of the large soft tissue defect of body. The rectus abdominis muscle is long and flat and it can be used as transverse or vertical myocutaneous flap. The TRAM flap is useful for moderate sized defects requiring a muscle or well vascularized tissue between Gracilis and Latissimus dorsi in size. We have experienced 6 cases of large soft tissue defect of the body using transverse rectus abdominis myocutaneous free flap. Advantages of this method are:enough available donor tissue, effect of abdominolplasty, no deformity of the donor site, hidden donor site scar, and blood supply by two arteries. Six patients showed successful results except one case of flap necrosis. The flap necrosis was due to hematoma around the vascular anastomotic site. We think reconstruction with TRAM flap is appropriately available for moderate sized soft tissue defect.

기저세포암 및 편평세포암에서 미세혈관밀도와 p53 단백 발현에 관한 연구

어수락,조규성,안호범,김대영,이삼용,조백현 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Basal cell carcinoma(BCC) and squamous cell carcinoma (SCC) are very prevalent neoplasms of the human skin. Ultraviolet radiation in sunlight is a well-established mutagen of the p53 gene and is one of basal cell carcinoma and squamous cell carcinoma. The newly-formed vascular network is important for neoplasms to grow beyond a size of about 1 ㎣. Recent reports have suggested the hypothesis that a mutant p53 protein is closely related with capillary density. Immumohistochemistry for p53 protein and CD34 was performed in 20 cases of BCCs and 14 SCCs to evaluated the relationship between p53 protein and capillary density. The results were as follows:1. The microvessels stained by CD34 were mainly located in the interface of tumor cells and stroma. 2. There was no difference in the microvessel density according to the histologic types and age of the patients, but a higher microvessel density was noted in male patients. 3. The aggressive BCCs and the less-differentiated SCCs showed higher p53 immunostaining. 4. The mean microvessel density of cases showing strong positive immunostaining of the p53 gene(54.73±17.75) was higher than that of others(39.75±18.30). These results suggested that p53 protein expression and microvessel density are not related to the histologic types and age of the patients, but that differentiation and biologic behavior such as the infiltrating property of tumors and the microvessel density are closely related to p53 protein expression.

일차성 구순열 성형술후 구순열 근육의 미세구조적 변화

조백현,조태영,안호범,김대영,이삼용,이민철 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.1

TThe cleft lip is the most common congenital craniofacial anomaly, the anatomical difference in operated patient and normal person by several authors. Authors deserved the ultrastructural changes in secondary cleft lip patients treated with primary cheiloplasty. We obtained eighteen muscle specimens from the upper lip of 18 patients during the time of secondary lip revision. It was stained with Hematoxylin Eosin, Epon-toluidine blue and histochemical method, and examined by light microscope. Also it was examined by electron microscope with various stain and several steps. We found the extensive collagenous fibrosis with various atrophy of muscles by light microscope, and the myofibroblast with rER, mitochondria, and abundant intermediate filaments by electron microscope in 14 cases. We also found muscular atrophy characterized with variable size of muscle fibers by light microscope, and marked variation of muscle fiber size with sarcoplasmic vacuoles, myocytolysis and internal migration of nuclei by electron microscope in 4 cases. In conclusion, in the most cases the major part of the tissues were surrounded by progressive fibrosis, the mild muscular atrophy was seen at some cases.