당뇨병 환자의 이하선 타액내 단백질의 다형현상에 대한 연구

안종모,윤창륙 朝鮮大學校 口腔生物學硏究所 1992 口腔生物學硏究 Vol.16 No.2

열처리한 치아에서의 개인식별을 위한 미토콘드리아 DNA 분석

안종모,윤창륙,김종열 대한안면통증∙구강내과학회 2002 Journal of Oral Medicine and Pain Vol.27 No.1

Teeth are relatively firm compare to other organs and stable to a variety of environment such as heat and putrefaction, therefore they are foresically significant samples in sex and age determination, blood typing and DNA typing. The author conducted the experiment on heated teeth to detect mitochondrial DNA related to individual identification in high temperature. The experiment was done on 33 teeth of unrelated Koreans consisted of heated ones to 100℃, 150℃, 200℃, 250℃, 300℃ for 1 hour and 2 hours respectively and to 350℃ for 1 hour. DNA was extracted from each tooth and amplified hypervariable region 1 of mitochondrial DNA by Polymerase Chain Reaction(PCR). Then mitochondrial DNA sequencing data was analysed according to the Anderson sequence and the followings are the results : 1. It was able to analyse mitochondrial DNA of 30 heated teeth to 300℃ for 2 hours. 2. It was comfirmed 29 mitochondrial lineages from 30 teeth of unrelated Koreans. 3. Among the sequences 45 nucleotide sites are polymorphic, 39 nucleotides of which is substituted by transition and 6 nucleotides by transversion. 4. The most frequent polymorphisms were found at the position 16,223(C to T transition) and 16,362(T to C transition) As a result, it is possible to extract DNA from charred teeth, therefore teeth can be used as a source of mitochondrial DNA for individual identification expecially when nuclear DNA is not extracted in charred body in high temperature.

구강 내 Helicobacter pylori

안종모,Ahn, Jong-Mo 대한안면통증구강내과학회 2012 Journal of Oral Medicine and Pain Vol.37 No.2

위염 및 위암 등의 발생과 관련되어 있는 Helicobacter pylori(H. pylori)는 구강의 치태와 타액에서 주로 발견이 된다. 유년시절 동안 주로 감염되는 것으로 알려져 있지만 감염경로는 불분명하다. 구강이 H. pylori의 두 번째 서식지로서 전염경로 및 위장내 H. pylori의 제균 후 재감염에 중요한 영향을 끼칠 수 있는지는 논쟁이 되고 있다. 따라서 본 저자는 문헌고찰을 통하여 구강 내에 존재하는 H. pylori에 관하여 알아보고자 하였다. 위장에 존재하는 H. pylori는 위인두반사나 구토에 의해 구강 내 발현될 수도 있으나, 구강과 위의 감염은 서로 관련성이 없는 것으로 보고되고 있다. 진단방법으로는 혈청학적검사, 요소호기검사, 중합효소연쇄반응(polymerase chain reaction: PCR)방법, Urease검사, 조직검사 등이 있으나, 타액과 치태에서는 nested PCR 방법이 주로 추천되어 진다. 구강 내 감염율은 다양하게 나타나며, 치과질환과의 연관성은 없는 것으로 사료된다. 그러나 치주질환 환자의 구강 내에서 발현율은 높게 나타나므로, 주의가 요구되며 향균 구강세척제의 사용이 권유된다. 결과적으로 구강 내 H. pylori는 정상세균총으로 사료되며, 향후 구강내 H. pylori에 관한 추가적인 많은 연구가 필요하리라 사료된다. Helicobacter pylori(H. pylori) associated with gastritis and gastric cancer is mainly detected dental plaque and saliva in the oral cavity. Most infection is probably acquired in childhood, but the route of transmission is not clear. The oral cavity has been indicated as secondary reservoir of H. pylori, and may therefore be argued in the route of transmission and reinfection of the stomach which follows treatment of H. pylori infection. So this review aimed to discuss about H. pylori in the oral cavity. H. pylori in stomach can appear in the oral cavity by gastroesophageal reflex or vomiting, but infection of stomach and oral cavity is different. Diagnostic methods are serological method, urea breath test, PCR method, urease test, histologic method and so on. Nested PCR recommend for detection of H. pylori in saliva and dental plaque. H. pylori infection in the oral cavity appear variously and is no relation with dental diseases. The antimicrobial mouthrinse recommend in patients with periodontal diseases because of high detection rate fo H. pylori. Thus H. pylori may be considered as the normal oral microflora.

선천성 매독환자의 치아 및 악안면 기형에 대한 임상적 연구

안종모,윤창륙 朝鮮大學校 口腔生物學硏究所 1991 Oral Biology Research (Oral Biol Res) Vol.15 No.-

A femle patient, 11 year-old, who referred from Department of orthodontics due to suggested congenital syphilis by Hutchinson incisor, and Hutchinson's incisor, mulberry molar, perioral rhagades and saddle nose, mandibular protuberance was examed clinically. Radiographs showed developing third molars and notch-shaped maxillary central incisors. Postitive reaction was observed by TPHA test & FTA-ABS test. According to the clinical, radiographical & clinicopathological findings, final diagnosis was established as late congenital syphilis. Late congenital syphilis is a rare transplacental infectious disease by infected materity with the common characteristic of marked frontal bossing, Hutchinson's teeth, interstitial ketatitis, 8th nerve deafness, saddle nose, mulberry molar, rhagades, saber shin, Clutton's joint etc. Patient was cared by administration of erythromycin per oral, but continuous careful follow-up was recommended for confirmation of serologic negative change.

섬유근통의 임상증례

안종모,윤창륙,허영돈 대한안면통증∙구강내과학회 2002 Journal of Oral Medicine and Pain Vol.27 No.2

Fibromyalgia is characterized by generalized pain and musculoskeletal tenderness, fatigue, nonrestorative sleep and morning stiffness. The causes of fibromyalgia are unknown, but the diagnosis is well established by the criteria of the American College of Rheumatology in the year 1990. The criteria for the diagnosis are widespread pain combined with tenderness at 11 or more of the 18 specific tender points. This article is a case report of a 18 year old male patient with fibromyalgia accompanied with temporomandibular disorders and describes the possible mechanisms and management of fibromyalgia.

Evaluation of Gustatory Function in Patients with Sleep Disordered Breathing

안종모,배국진,윤창륙,유지원 대한안면통증∙구강내과학회 2014 Journal of Oral Medicine and Pain Vol.39 No.1

Purpose: The aim of this study is to evaluate the difference between gustatory functions in a sleep disordered breathing (SDB) group and a control group. The pathogenesis of SDB has not been fully understood. Though the precise contributions of neuromuscular and anatomical factors on SDB pathogenesis are still debated, we hypothesized that the gustatory dysfunction could be predisposed to SDB. Methods: All patients were diagnosed as SDB by polysomnography (PSG). On the basis of PSG results, patients were divided into 3 groups: snoring, mixed, and obstructive sleep apnea (OSA). The control group comprised healthy volunteers who were the same age as those of the SDB group and whose breathing was verified as normal using a portable sleep monitor device. The patient group and the control group were evaluated for gustatory functions with an electrogustometry (EGM). The electrical taste thresholds were measured in the anterior, midlateral, and posterior sides of the tongue and soft palatal regions, both sides. To find out the difference in EGM scores, statistical analysis was performed using the Kruskal-wallis and Mann-Whitney U test with 95% confidence interval and p<0.05 significance level. Results: The patients with SDB had higher EGM scores than the control group at all spots tested, except for the right midlateral of the tongue, and there was a statistical significance in the comparison between the control group and the divided SDB groups, respectively. Among the divided SDB groups, the snoring group had the most significant differences in the number of the measured spots, but there was no difference among the snoring, mixed, and OSA groups. Conclusions: These results may suggest that neurologic alterations with sleep disordered breathing could be associated with gustatory dysfunction. In the future, further systemic studies will be needed to confirm this study.

열처리한 치아에서 미토콘드리아 DNA와 핵 DNA 분석

안종모,허웅,윤창륙 대한안면통증∙구강내과학회 2004 Journal of Oral Medicine and Pain Vol.29 No.2

The teeth are the hardest substances in the human body and stable tissue to a variety of environmental change such as heat and putrefaction. Therefore, they are forensically significant material for individual identification. The auther conducted the experiment to analysis mitochondrial DNA(mtDMA) and nuclear DNA on heated teeth. The experiment was done on 16 teeth of unrelated koreans, they are classified 4 groups (100℃, 200℃, 300℃, 400℃ by temperature) each 4 teeth and heated for 1hour. DNA was extracted from each tooth and amplified by polymerase chain reaction. The following is the result : We can observed amplified product of hypervariable control region Ⅱ of mtDNA on all teeth. And amelogenin gene and seven STR loci[CTT(CSF1PO, TPOX, TH01), FFV(F13A01, FESFPS, vWA), F13B] are amplified only 4 teeth heated on temperature of 100℃ . As a result, we know that the mtDNA is extracted high temperature than nuclear DNA. And mtDNA analysis for individual identification is useful method especially when nuclear DNA is not extracted in charred body in high temperature.

성인형 치주염 환자의 타액 및 치은연하치태에서 Helicobacter pylori의 발현양상

안종모,나명수,김병옥,An, Jong-Mo,Na, Myoung-Su,Kim, Byung-Ock 대한치주과학회 2004 Journal of Periodontal & Implant Science Vol.34 No.4

Helicobacter pylori(H. pylori) has been associated with the cause of chronic gastritis, peptic ulcers and gastric cancer. Although it may be transmitted through the oral cavity, it is unknown whether the oral cavity acts as a reservoir of H. pylori. The purpose of this study was to investigate the mode of detection of H. pylori in oral cavity of adult periodontitis patients with plaque and periodontal pocket which atmosphere is grown well H. pylori. We analysed detection rate of H. pylori in saliva and subgingival plaques of 17 adult periodontitis patients without symptoms of gastroduodenal disease by nested PCR. Samples tested comprised saliva and subgingival plaques from central incisor, 1st premolar and 1st molar. H. pylori DNA was not identified in saliva from all patients. The detection rate in subgingival plaque from incisors, premolars and molars was 5.9%, 5.9% and 17.7%, respectively. In conclusion, the dental plaque and periodontal pocket (especially, of molars) in adult periodontitis can be favorable reservoir of H. pylori and may be the source of infection and transmission of H. pylori.

Orofacial Pain and Nonodotogenic Toothache of Cardiac Origin: Case Report

안종모,유지원,박현정 대한안면통증∙구강내과학회 2024 Journal of Oral Medicine and Pain Vol.49 No.1

Orofacial pain has various causes, making it challenging to differentiate from dentalrelated diseases based solely on symptoms. Toothache, usually attributed to pathological changes in the pulp and periodontal tissue, is the most common cause of orofacial pain and relatively easy to diagnose. However, distinguishing orofacial pain and nonodontogenic toothache due to myofascial, neuropathic, neurovascular, paranasal sinus and cardiac originating, and psychogenic pain presents diagnostic challenges that may result in incorrect treatment. Therefore, dentists must recognize that orofacial pain can arise from not only dental issues but also other causes. This case report explores the necessary considerations in diagnosing orofacial pain and nonodontogenic toothache by examining the diagnoses of patients presenting at the dental hospital with orofacial pain and nonodontogenic toothache of cardiac origin.

삼차신경통 환자의 임상적 특징 및 관리

안종모 ( Jong Mo Ahn ),김준호 ( Jun Ho Kim ),홍성주 ( Seong Ju Hong ) 조선대학교 치의학연구원 2011 Oral Biology Research (Oral Biol Res) Vol.35 No.2

Trigeminal neuralgia (TN) is a severe unilateral paroxysmal, lacinating facial pain. The diagnosis of TN is based on several characteristic clinical features. The purpose of this study was to evaluate the clinical manifestations of TN patients treated at the Department of Oral Medicine, Chosun University Dental Hospital. Materials and Methods: A total of 56 patients with TN from January 2008 to December 2009 was included for this study. We investigated the gender, age and systemic diseases of patients, the sites of pain, the methods and duration of treatment and the course that comes to department of oral medicine. 89.3% of the patients were over 40 years of age and 64.3% were women. Results: Patients with TN had mainly diseases of the circulatory system and endocrine diseases. The two most frequently involved trigeminal nerve brandches were mandibular (53.6%) and maxillary (32.1%) branches. 94.6% of the patients were treated by medication and 92.8% of the patients controlled the pain within 6 months. And we could know that 32.1% of the patients were comes to the Department of Oral Medicine within 2 months after occurrence of the symptom and 89.3% of the patients were comes to the Department of Oral Medicine via other clinics or hospitals and other Department of Chosun Univerisity Dental Hospital.