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청리자감탕(淸離滋坎湯)의 폐암 세포주 A549의 invasion activity 억제 효과
심범상,김성훈,최승훈,안규석,Shim, Bum-Sang,Kim, Sung-Hoon,Choi, Seung-Hoon,Ahn, Koo-Seok 대한암한의학회 2001 大韓癌韓醫學會誌 Vol.7 No.1
By applying in vitro invasion assay model, we examined the anti-metatstastic effect of ChunghjagamTang(CLJGT). In 3H-thymidine incorporation assay, CLJGT treated groups showed the decreased DNA synthesis rate compared with control group. Gelatin zymogram assay showed that CLJGT decreases the gelatinolytic activity of MMP-9 from A-549, at the concentration of $800{\mu}g/ml$. We examined whether CLJGT inhibits the invasion of A-549 cells through the matrigel precoated transwell chamber. The results showed that CLJGT effectively inhibited the invasion of A-549 as compared with the control (+PMA) groups. From our research, part of mechanism underlying anti-metastastic effect of CLJGT was proven in vitro.
행인 제제의 독성 및 항암효과의 자연살해세포 활성에 미치는 효과
심범상,최승훈,박재경,Shim, Bum-Sang,Choi, Seung-Hoon,Park, Jai-Kyung 대한암한의학회 2000 大韓癌韓醫學會誌 Vol.6 No.1
In this experiment, we examined the the effect of Laetrile Oil to the immune system and tried to disclose the anti-cancer mechanism of this. The result is listed as below. 1. After per-os, sub-cutaneous and into the peritoneal injection of Laetrile Oil to the SPF mice, the LD50 is above 5000mg/kg at even group. 2. Mean survival days of mice treated with laetril oil, after S-180 cells transplantation into the peritoneal cavity decreases 1.5 days(-6.8%) compared with the Mean survival days of the control group(22days.) 3. Effects of laetril oil on natural killer cell activity at Effector/Target Cell Ratio with 100:1, 50:1, 10:1 into methotrexate-pretreated mice is like this.: Compared with $29.22\pm12.7\%$ Cytotoxicity of the control group, sample group's Cytotoxicity had $38.83{\pm}12.5%$ of meaningful acrease. At 100:1 Effecr/Target Cell Ratio. At 50:1 Effector/Target Cell Ratio, control group has $20.02{\pm}9.6%$ Cytotoxiciy and sample group had $31.53{\pm}13.4%$ Cytotoxicity. At 25:1 Effector/Target Cell Ratio control group has $13.60{\pm}6.6%$ Cytotoxicity and sample group had $20.81{\pm}9.8%$ Cytotoxicity According to the above results, the Laetrile Oil represents nontotoxic to a SPF mice. non-effective to transplantable Sarcoma 180 tumors, and activaion in NK cell activity.
행인 제제의 독성 및 항암효과와 자연살해세포 활성에 미치는 효과
심범상,최승훈,박재경 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1
In this experiment, we examined the the effect of Laetrile Oil to the immune system and tried to disclose the anti-cancer mechanism of this. The result is listed as below. 1. After per-os, sub-cutaneous and into the peritonal injection of Laetrile Oil to the SPF mice, the LD50 is above 5ooomg/kg at even roup. 2. Mean survival days of mice treated with laetril oil, after S-180 cells transplantation into the eritoneal cavoty decreases 1.5 days(-6.8%) compared with the Mean survival days of the control group(22days.) 3. Effects of laetril oil on natural killer cell activity at Effector/Target Cell Ratio with 100:1, 50:1, l0:l into methotrexate-pretreated mice is like this.: Compared with 29.22±l2.7% Cytotoxicity of the control group, sample group's Cytotoxicity had 38.83±l2.5% of meaningful acrease. At l00:1 Effecr/Target Cell Ratio. At 50:l Effector/Target Cell Ratio, control group has 20.02±9.6% Cytotoxiciy and sample group had 3l.53±13.4% Cytotoxicity. At 25:l Effector/Target Cell Ratio, control group has 13.60±6.6% Cytotoxicity and sample group had 20.8l±9.8% Cytotoxicity. According to the above results, the Laetrile Oil represents nontotoxic to a SPF mice. non-effective to transplantable Sarcoma 180 tumors, and activaion in NK cell activity.
심범상,강희,김성훈,안규석 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5
Chunglijagam-tang(CLJGT)'s immunomodulating effects were examined using the spleen cells obtained from immunosuppressed ICR mice which were orally administered with CLJGT for 3 weeks and the spleen cells from normal Balb/c mice. Higher levels of T cell receptor expression was induced in the CLJGT group compared with the control group. The proliferative capacity of spleen cells cultured for 24 hour in the media containing serum obtained from the CLJGT group was also increased compared with the control group. Using the in vitro model in which Balb/c murine spleen cells were stimulated with concanavalin A, the CLJGT group showed a slightly increased expression of CD4+ helper T cells. However, the levels of IL-2 production in the supernatant of polyclonally activated spleen cells did not differ between the control and the sample. In conclusion, the immunological effect of CLJGT was mainly exerted in immunosuppressed status, but it did not seem to play a significant role in immune-boosting aspects.
崔昇勳,沈範相 대한동의병리학회 1993 동의생리병리학회지 Vol.8 No.-
연구배경 : 최근 생활상의 변화에 따른 종양의 발생빈도가 증가하고 있으며 이에 따른 각종 임상보고 및 실험보고가 활발하게 이뤄지고 있으나 암에 대한 동서의학간의 인식과 접근의 차이에 따라 실제 임상상에서 많은 혼란이 일고 있다. 가령 胃癌의 경우 이에 해당하는 한의학적 병증으로는 積聚, 비하, 현벽, 反胃, 伏梁, 胃脘通, 심하비 등이 거론될 수 있으며 각각의 변증분형까지 살펴본다면 더욱 혼란스럽다. 이에 따라 본 논문에서는 위암의 변증분형을 예로 삼아 문헌적인 고찰을 통해 그 규범화 가능성을 살펴보았다. 방법 : 위암의 주된 증상중에서 한의학적 병증으로 대표적이라고 생각되는 위완통, 반위의 병인병기 및 변증분형을 고찰하였고 이를 현대 중국의 중서외결합에 의한 변병의 기초위에서 행해진 위암의 변증분형과 상호 비교하였다. 결과 : 병인병기 방면에서는 대개 위암, 위완통, 반위가 별 차이없이 중복되고 있으나 변증분형에서는 위완통의 경우 氣滯(肝胃不和)만이, 반위의 경우 反胃初氣를 제외한 脾胃虛寒, 命門火衰, 眞陰枯虧, 酒濕傷脾 등이 위암의 변증분형과 대체로 일치하고 있다. 결론 : 위완통은 早期胃癌에, 반위는 進行癌 혹은 胃癌의 末期에 상당하는 것으로 생각된다. 위암의 발생과 발전 등에 따른 病期別로 해당하는 한의학적 병증들에 대한 문헌적 비교고찰이 필요하며 보다 심화된 연구와 변증분형의 규범화를 위해서는 정확한 임상적 보고가 필요한 것으로 생각된다.
혈부축어탕(血府逐瘀湯)이 암전이(癌轉移) 억제(抑制)에 미치는 영향(影響)
이진화,심범상,안규석,최승훈,Lee, Jin-Hwa,Shim, Bum-Sang,Ahn, Kyoo-Seok,Choi, Seung-Hoon 대한암한의학회 1999 大韓癌韓醫學會誌 Vol.5 No.1
To examine the effect of Xuefuzhuyutang on the metastasis of cancer, the following experiments were carried out. Before the main experiments, the cytotoxicity was measured by putting Xuefuzhuyutant sample in HT1080. Then zymography was made to examine the change of gelatinolytic activity. Western blotting was carried out to examine the changes of Fos, Jun, Ets, Erk, md JNK. In vitro invasion assay with transwells coated by collagen and matrigel was carried out. From the above results the following conclusions were obtained. 1. The experimental result about cytotoxicity of Xuefuzhuyutang agaitst HT1080 was a below. The stained cell count after being treated by by Xuefuzhuyutang sample $400{\mu}g/ml$ for 24 hours was 0.9% of total cells, and the stained cell count by Xuefuzhuyutang sample $100{\mu}g/ml$ was 1.5% of total cells. Both were near the level of control group which showed 0.6% stained. 2. The result of collagenase assay was as below. In Xuefuzhuyutang sample $400{\mu}g/ml$, MMP2 was reduced as compared with TPA control group, and the band of MMP-9 induced by TPA disapappeared. In Xuefuzhuyutang samle $800{\mu}g/ml$ both bands of MMP-2 and MMP-9 disapeared. 3. The results of western blots for Jun, Fos, Ets, Erk, JNK were a below. In Xuefuzhuyutang sample $200{\mu}g/ml$, Ets was reduced, and Jun, Fos were increased. 4 The result of invasion assay was as below. The number of cells which migrated across transwell membrane in Xuefuzhuyutang-treated group was less than that of control(+TPA) group. From the above results, it was concluded that Xuefuzhuyutang might inhibit the activity of collagenase not by the MMP-2, MMP-9 promoter but by the other way.