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수중걷기운동이 비만중년여성의 스트레스호르몬 및 기분상태변화에 미치는 영향
신현경(Sin Hyun-Kyung),김종혁(Kim Jong-Hyuck),김수경(Kim Soo-Kyung) 한국체육과학회 2008 한국체육과학회지 Vol.17 No.2
This study focuses on the effect of Aquatic-walking exercise on the Stress Hormone and Mood State Changes. This study was performed for the purpose of providing the basic data to develop adequate exercise program and prescription for decreasing the rate of Women's obesity and Coronary Artery Disease by operating exercise program targeted on 20 middle-aged women with 30% over body fat rate. The Aquatic-walking excercise for selected targets had been proceeded for 8 weeks, 3 times in a week. This exercise program was required total 40 minutes and consisted free gymnastics and stretching for 5 minutes as Warm-up , Aquatic-walking for 30 minutes as Main exercise and again free gymnastics and stretching for 5 minutes as cooling-down. The intensity was elevated by wearing Polar on exercising. The intensity was at 40~50% HRmax from the first to second weeks, 50~60% HRmax from the third and sixth weeks and 60~70% HRmax from the seventh and eighth weeks. The result of performed study was as below. First, there were significant differences(P<.001) on all of the factors like Stress Hormone, Cortisol, Epinephrine and Norepinephrine. Second, there were significant differences on Insecurity, Depression, Anger, Vitality and Fatigue among Mood State Changes. But there was no significant difference just on Confusion. Therefore, the 8 weeks' Aquatic-walking program had positive effects on almost every factor of Stress Hormones and Mood State Changes of middle-aged women with obesity. Through this result, it is considered that the program is able to positively affect on Weight Loss and prevention of Coronary Artery Disease and the continuous study on this is needed.
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오윤신(Yoon Sin Oh),김은지(Eun Ji Kim),이상곤(Sang Gon Lee),정차권(Cha-Kwon Chung),강일준(Il-Jun Kang),신현경(Hyun Kyung Shin),윤정한(Jung H. Y. Park) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.6
CLA 이성체 mixture와 trans-10,cis-12 CLA는 살아있는 TSU-Pr1 세포 수를 감소시키는 반면 LA와 cis-9,trans-11 CLA는 세포 수에 아무런 효과가 없었다. 세포 수를 감소시키는 CLA 이성체 mixture와 trans-10,cis-12 CLA는 apoptotic cell death를 유도하는 것을 관찰하였고, 특히 trans-10,cis-12CLA는 Bcl-2/Bax 비율을 감소시켰다. Trans-10,cis-12 CLA에 의해 Bax와 Bcl-2 mRNA의 수준이 변하지 않았으므로 이 지방산에 의한 Bcl-2의 변화는 mRNA 전사 후에 일어나는 단계에서 일어난다고 결론지을 수 있다. 이로써 CLA에 의한 세포증식 억제는 부분적으로 TSU-Pr1 세포의 apoptosis를 유도함으로서 이루어지는 것이라 할 수 있다. Conjugated linoleic acid (CLA) is a collective term for a class of positional and geometric conjugated dienoic isomers of linoleic acid (LA) and has anti-cancer activity in experimental animals. We have previously observed that an isomeric mixture of CLA and trans-10,cis-12 (t10c12) inhibited cell growth in a dose-dependent manner, whereas LA and cis-9,trans-11 (c9t11) had no effect. The present study examined whether the CLA mixture and t10c12 induce apoptotic cell death. TSU-Pr1 cells were incubated for three days in serum-free medium in the absence or presence of individual fatty acids, and the DNA fragmentation assay was performed. Cells treated with the CLA mixture or t10c12 produced a distinct oligonucleosomal ladder with different sizes of DNA fragments, a typical characteristic of cells undergoing apoptosis. By contrast, LA and c9t11 had no effect. Western immunoblot analysis of total lysates revealed that t10c12 reduced anti-apoptotic, 26 kDa, Bcl-2 protein levels by 49±8% compared with controls, whereas this CLA isomer did not alter pro-apoptotic, 21 kDa, Bax protein levels. These results suggest that growth inhibitory effect of the t10c12 CLA isomer may, at least in part, be attributed to increased apoptotic death in TSU-Pr1 cells.
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