http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
온오프 네트워크 구축을 통한 농촌지역 커뮤니티 비즈니스 활성화 방안 연구 : 경남 산청군 민들레 공동체 사례를 중심으로
신용욱,박상혁 (사)디지털산업정보학회 2012 디지털산업정보학회논문지 Vol.8 No.3
This study intends to find a solution through the activation of community business as a business as a business model of the creation of real satisfaction for the region’s residence. In this study focuses on finding out how community building project, which is recently emerging as a new paradigm of regional development. In the past, we have lived community units, but nowadays many communities have been destroyed by the capitalization and the urbanization even in the rural area. This report studies essential elements needed for successful 'Rural Community Business' through case studies of 'Dandelion Village' in Gyoengsangnamdo. In order to do so, we analyzed this villages according to a theoretical 'Sustainable Community Business Model.' We constructed successful business model of 'Rural Community Business' based on six groups - 'Community Leader,' 'Teamwork,' 'Sustainable Population Influx,' 'Sustainable Voluntary Participations of Community Members,' and 'Sustainable Networking.'
다공성 SiO<sub>2</sub>/ITO 나노박막의 전기적 특성
신용욱,김상우,Sin, Yong-Uk,Kim, Sang-U 한국재료학회 2002 한국재료학회지 Vol.12 No.1
The electrical properties of porous $SiO_2/ITO$ nano thin film were studied by complex impedance and conductive mechanisms were analyzed. According to the results of complex impedance, the activation energy of $SiO_2/ITO$ and $Zn-SiO_2/ITO$ were 0.309 eV, 0.077 eV in below $450^{\circ}C$ and 0.147 eV in over $450^{\circ}C$, respectively. In case of $SiO_2/ ITO$, slightly direct tunneling occurred at room temperature. The contribution for conduction was very tiny because of high barrier of silica. However, the conductivity abruptly increased in over $300^{\circ}C$ by Thermally assisted tunneling. In case of $Zn-SiO_2/ITO$, high conductivity in 1.26 ${\Omega}^{ -1}{cdot}cm^{-1}$ at room temperature appeared by space charge conduction or Frenkel-poole emission that Zn ions play a role as localized electron states.
분자진화 기술을 통한 Vibrio metschnikovii 유래 고활성 알칼리성 단백질 분해효소 생산균주 개발
신용욱,이과수,조재형,이현환,Shin, Yong-Uk,Lee, Gwa-Soo,Jo, Jae-Hyung,Lee, Hyune-Hwan 한국미생물학회 2010 미생물학회지 Vol.46 No.4
알칼리성 단백질 분해효소 고생산 돌연변이 균주 Vibrio metschnikovii L12-23, N4-8, KS1으로부터 알칼리성 단백질 분해효소를 암호화하는 vapK (Vibrio alkaline protease K) 유전자들을 PCR에 의하여 분리한 다음 DNA shuffling, error-prone PCR 방법과 같은 분자진화 기술을 통해 고활성 단백질 분해효소를 생산하는 재조합 V. metschnikovii 균주를 제작하였다. DNA shuffling 방법을 통해 변형시킨 vapK-1 유전자와 이 유전자를 주형으로 error-prone PCR 기법을 통해 재 변형된 vapK-2 유전자를 cloning한 후 V. metschnikovii KS1 균주에 역도입하여 재조합 균주를 제조하였다. 재조합 균주들의 단백질 분해 능력을 조사한 결과 vapK-2 유전자가 2 copy 도입된 재조합 균주의 경우 야생형 균주인 V. metschnikovii RH530에 비해 43.6배 높은 단백질 분해활성을 보였으며 숙주인 V. metschnikovii KS1에 비해 약 3.9배 향상된 단백질 분해 활성을 확인할 수 있었다. 변형된 vapK-1과 vapK-2 유전자를 야생형 vapK 유전자의 염기서열을 비교 분석한 결과 단백질 분해 능력의 활성에 영향을 미치는 active site를 제외한 부분에서 변화가 일어났음을 확인 할 수 있었다. 변형된 유전자 vapK-1을 two copy를 포함한 재조합 플라스미드를 가진 V. metschnikovii KS1을 30 L fermentor로 배양 하였을 때 배양 후 35 시간에 18,000 PU/ml의 활성을 보였으며, 이는 향후 산업용 균주로서 사용될 수 있는 가능성을 제시하였다. Alkaline protease-overproducing strains of Vibrio metschnikovii were developed by using the molecular evolution from the classical mutants V. metschnikovii L12-23, N4-8, and KS1. Each vapK (Vibrio alkaline protease K) was obtained from the genomic DNAs of mutants by PCR to carry out the DNA shuffling. The modified vapK-1 obtained by DNA shuffling was used again as a template for the error-prone PCR to make the vapK-2. Both genes were cloned in the plasmid pKF3 to construct the recombinant plasmids which have one or two copies of the modified genes. The recombinant plasmids were back-transformed to V. metschnikovii KS1 to construct recombinant V. metschnikovii that expresses the alkaline protease. About 3.9-fold more protease activity was measured in the strain which has the plasmid containing two copies of vapK-2 when compared to strain KS1. When compared to wild type V. metschnikovii RH530, 43-fold more activity was achieved. Comparison of amino acids among vapK, vapK-1, and vapK-2 revealed that the active sites was highly conserved and not changed. However, many amino acids except the active sites were changed. These results suggested that the changes in amino acids might play an important role in the increase of protease activity by allowing the easy access of substrate to active sites of the protease. The fermentation of alkaline protease from the V. metschnikovii KS1 harboring the plasmid that contains two copies of vapK-1 showed the possibility of this strain to be used as industrial producer.
신용욱,김치환,한진태 대한물리치료학회 2020 대한물리치료학회지 Vol.32 No.2
Purpose: This study examined the effects of the directions of neck rotation on the muscle activity of the upper trapezius and lower trapezius while rotating a shoulder externally. Methods: Twenty-five healthy males participated in this study. The subjects were asked to rotate their shoulder externally with 90° shoulder abduction and 90° elbow flexion in three different neck rotations (neutral, ipsilateral, and contralateral) in the prone position. The muscle activities of the upper and lower trapezius were measured using surface electromyography. One way repeated measures ANOVA was used to compare the muscle activity of the upper and lower trapezius depending on the different neck turning directions. Results: In the upper trapezius, turning the neck in the ipsilateral direction while turning a shoulder externally decreased the muscle activity significantly, but the muscle activity was increased significantly by turning the neck in the contralateral direction. On the other hand, in the lower trapezius, turning the neck in the ipsilateral direction increased the muscle activity significantly, but the muscle activity was decreased significantly by turning the neck in the contralateral direction decreased it significantly. Conclusion: When someone has an imbalance of shoulder function, turning the neck in the ipsilateral direction while turning the shoulder externally in the prone position is effective in decreasing the activity of the upper trapezius and increasing the activity of the lower trapezius. Therefore, these results could be used as basic evidence for researching patients with shoulder problems.