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Ribosomal DNA 제한효소 분해양상을 이용한Acinetobacter baumannii 동정
신명근,김수현,이제철,조덕,기승정,신종희,서순팔,양동욱 대한진단검사의학회 2004 Annals of Laboratory Medicine Vol.24 No.2
Background : The glucose -acidifying genomic species 1, 2, 3 and 13 of the genus Acinetobacter are highly related genetically and may be difficult to differentiate by phenotypic identification schemes using biochemical tests. The aim of this study was to explore the brief restriction enzyme profiles of amplified ribosomal DNA restriction analysis (ARDRA) to identify medically important species of Acinetobacter. Using ARDRA analysis, we evaluated the ID 32 GN system (bioMerieux, Lyon, France) for the identification of A. baumannii (genospecies 2). Methods : A collection of 78 A. baumannii stains initially identified by the ID 32 GN system was used to determine its accuracy by ARDRA analysis. ARDRA was performed with 10 different restriction enzymes, AluI (AGCT), CfoI (GCGC), HaeIII (GGCC), HinfI (GANTC), MboI (GATC), MspI (CCGG), NciI (CCGG), RsaI (GTAC), ScrFI (CCNGG) and TaqI (TCGA). Results : The combination of restriction patterns obtained with respective enzymes AluI, CfoI and MboI allowed for the discriminatory value for the identification of medically important genospecies such as genospecies 2 (A. baumannii), 3 and 13. By comparing ARDRA results of the 78 strains previously identified by ID 32 GN system, we found the correlation rate between the two systems to be 88.5% (69/78). Nine strains were identified as Acinetobacter genospecies 13 by ARDRA. Conclusions : This result suggests that the ID 32 GN system may have difficulty in discriminating A. baumannii from genospecies 13. This revised ARDRA method gives a relatively rapid and definitive result for the identification of medically important genospecies of Acinetobacter. (Korean J Lab Med 2004; 24: 107-12) 배경 : 임상에서 유래하는 대부분의 Acinetobacter 균주는 유전형 2 ( A. baumannii ), 3 및 13이다. 그러나 이들은 유전적으로매우 상동성이 높아 통상의 생화학적 방법으로 정확하게 동정하는것은 어렵다고 알려져 있다. 따라서 본 연구에서는 임상적으로 중요한 이들 균주를 ribosomal DNA제한 효소 양상(amplified ribo-somalDNA restriction analysis, ARDRA)을 이용하여 비교적간편하게 유전형을 판정할 수 있는 최소/최적의 제한효소 조합을고안하고자 하였다. 그리고 이를 이용하여 ID 32 GN system의A. baumannii 동정능을 비교 검토 하였다. 대상 및 방법 :각종 임상검체에서 ID 32 GN system에 의해동정된 총 78주의 A. baumannii 를 이용하였다. 한편 새로 간편하게 고안된 ARDRA 분석법의 정확도를 평가하기 위하여 표준균주로 A. baumannii (ATCC 19606) 및 Acinetobacter genospecies13 (ATCC 17803)를 사용하였다. 한편 임상에서 중요한 3가지 주요 균주 동정을 위한 최소/최적 제한효소 조합의 ARDRA 방법을확립하기 위해 총 10가지(AluI, CfoI, HaeIII, HinfI, MboI, MspI,NciI, RsaI, ScrFI 및 TaqI)의 제한 효소를 일차 검사하였다.결과 :ARDRA 방법으로 Acinetobacter 유전형 2, 3 및 13을동정하기 위한 최소/최적의 제한효소 조합은 AluI, CfoI 및 MboI이었다. ID 32 GN에 의해 동정된 78주의 A. baumannii 중 9 주는 Acinetobacter 유전형 13으로 판명되었다. 따라서 두 동정 방법간의 일치율은 89% (69/78)였다. 결론 : 이상의 결과로 ID 32 GN system은 비교적 높은 A. baumannii 동정능을 보여 주었으나 유전형 13과의 정확한 분리동정능에는 일부 한계가 있음을 시사하였다. 한편 제안된 ARDRA방법으로 비교적 간단하고 정확하게 임상에서 중요한 Acinetobacter 유전형을 결정할 수 있었다.
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신명근,신승헌,손병창 대한인간공학회 2008 대한인간공학회 학술대회논문집 Vol.- No.-
본 연구는 중심이 동요될 때 지지구의 효과에 대하여 중심동요계를 이용하여 고령자를 중심으로 검토한 것이다. 측정조건으로는 양족개안, 편족폐안으로 하였고, 검토 parameter는 궤적장과 동요면적으로 했다. 피험자는 60연령대 8명, 20연령대 8명으로 하였으며, 20연령대 피험자는 60연령대와 비교하기 위하여 채택했다. 먼저 양족개안에서 궤적장은 지지구의 효과가 그다지 반영되지 않았으나 편족개안에 있어서는 그 효과가 크게 반영되었다. 동요면적에 있어서는 궤적장과 유사한 결과가 얻어졌으나 변화의 폭(지지구의 효과)이 가장 컸고, 표준편차가 크게 나타나 개인차이가 잘 반영되었다. 이번 실험에서 고령자가 되면 근력이 쇠퇴되어 편족직립이 어렵다는 것을 알 수 있었고, 고령자들에게 편족직립 자세를 요구하는 상황(예: 계단을 오름, 신발을 신음, 옷을 입는 등)에서는 지지구, 난간대등이 필요하다는 것을 알 수 있었다.
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골수, 말초혈액 및 제대혈 단일 CD34+ 세포의 성장 및 분화능 비교 연구
신명근,조덕,이진솔,최현우,오봉준,김수현,기승정,신종희,서순팔,양동욱 대한혈액학회 2004 Blood Research Vol.39 No.4
Background:Although bone marrow (BM) CD34+ cells and peripheral blood (PB) CD34+ cells are developmentally and functionally related, the recent data suggested that they may have different functional and clinical capabilities. Moreover, they have differential gene expression underlying the functional distinctions of primary human CD34+ hematopoietic stem and progenitor cells from BM and PB. The aim of this study was to investigate the plating efficiency of single CD34+ progenitor cell from BM, PB and umbilical cord blood (UCB). Methods:After sorting, single CD34+ cells were cultured in individual wells of 96-well plates in serum-free medium containing selected hematopoietic growth factors, with or without G-CSF. Plating efficiency was microscopically determined by the presence of clusters of viable cells:[the number of positive (cells were present) wells÷total wells]×100. CD34+ cell-derived colonies were classified according to the cell number per well. Results:Although there was some variation of plating efficiency of CD34+ cells among six normal BMs, six PBs and five UCBs, overall average plating efficiency of single CD34+ cells from BM, PB and UCB was 30% (30.0±11.7, mean±SD), 79% (78.6±11.7) and 45% (45.3±9.3) respectively. As expected, the colony size was increased in the presence of G-CSF. Conclusion:The results of this study clearly showed that the different ex vivo expansion of single CD34+ progenitor cells from BM, PB and UCB. These might be an important data for understanding stem cell expansion in vivo and designing clinical application.
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신명근 대한의사협회 2014 대한의사협회지 Vol.57 No.4
Aging is an universal phenomenon and irreversible syndrome, and its damage occurs to molecules (DNA, proteins,and lipids), to cells, and to organs. Hematopoietic tissue intrinsically has a very high turnover rate; nonetheless, it is notprotected from age-related insults. Aging results in the overproduction of myeloid cells, which leads to a pro-inflammatoryenvironment. The selective expansion of a clonal subtype of intrinsically myeloid-biased hematopoietic stem cells (HSCs)is a central component of hematopoietic aging. In the present study, the stress-response and inflammatory genes wereup-regulated with age whereas chromatin remodeling and DNA repair genes were down-regulated. Accumulated DNAdamage, loss of DNA repair, and epigenetic deregulation are the main molecular mechanisms underlying age-dependentHSC decline. The most profound effect is seen in the adaptive immune system with a marked decline of lymphoid functionin the elderly. Mitochondrial dysfunction and mitochondrial DNA mutation are another important contributor to the agingof HSCs, which have been regarded as a part of the mitochondrial theory of aging. Generation of reactive oxygen speciesduring mitochondrial adenosine triphosphate generation, results in damage to mitochondria and mitochondrial DNA, thelatter leading to deleterious mutations that directly caused the functional decline of human. Studies have pointed towardintrinsic deficits in HSC function, and epigenetic deregulation as the important contributing factors behind hematopoieticdecline and malignancy during aging. Aging-related changes such as hematopoiesis are reflected by a decline in marrowcellularity, increased risk of anemia, marrow failure syndrome, and myeloproliferative neoplasms as well as a decline ofadaptive immunity.
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지식 추상화와 의미 거리 접근법을 통합한 질의 완화 방법론
신명근,허순영,박성혁,이우기 한국경영과학회 2007 韓國經營科學會誌 Vol.32 No.1
The study on query relaxation which provides approximate answers has received attention. In recent years, some arguments have been made that semantic relationships are useful to present the relationships among data values and calculating the semantic distance between two data values can be used as a quantitative measure to express relative distance. The aim of this article is a hierarchical metricized knowledge abstraction (HiMKA) with an emphasis on combining data abstraction hierarchy and distance measure among data values. We propose the operations and the query ralxation algorithm appropriate to the HiMKA. With various experiments and comparison with other method, we show that the HiMKA is very useful for the quantified approximate query answering and our result is to offer a new methodological framework for query relaxation.
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