실험적으로 요도협착을 만든 백서에서 Mitomycin-C의 효과

신대은,김철성,노준,문형윤 대한비뇨의학회 2009 Investigative and Clinical Urology Vol.50 No.8

Purpose: Urethral stricture is a fibrotic process and, although it is one of the oldest known urologic diseases, it remains a common problem with a high recurrence rate. Mitomycin-C has been reported to have anti- replication properties in animal and clinical studies. The aim of this study was to observe the intraurethral impact of the use of mitomycin-C on scar formation and fibrosis in an experimental rat model of urethral stricture. Materials and Methods: Twelve male Sprague-Dawley rats were used. With the animals under deep anesthesia, an internal urethrotomy was made with a cold knife. After the urethral injury, the rats were grouped randomly as follows: group 1 (control, n=4), group 2 (3 mg/l of mitomycin-C, n=4), and group 3 (5 mg/l of mitomycin-C, n=4). The rats were sacrificed 14 days later to evaluate epithelial proliferation and fibrosis. The penile urethra was removed and histopathologically examined by H&E staining, Masson trichrome staining, and immunohistochemistry with anti-collagen type I antibody. Results: The stained specimens were examined under a light microscope. The extent of fibrosis and re-epithelization after urethral injury was greater in the areas with trauma than in those without. These findings were significantly reduced in the groups treated with mitomycin-C as compared with group 1, but there was no statistical difference between group 2 and group 3. Mitomycin-C treatment also prevented increases in collagen type I, whereas group 1 showed increases in collagen type I and collagen contents at the stricture site. Conclusions: These results suggest that mitomycin-C might inhibit the renewal of the epithelium and the synthesis of collagen secreted by fibroblasts in the affected urethra and then prevent scar formation. This raises the possibility of the use of mitomycin-C to prevent urethral stricture caused by trauma. Purpose: Urethral stricture is a fibrotic process and, although it is one of the oldest known urologic diseases, it remains a common problem with a high recurrence rate. Mitomycin-C has been reported to have anti- replication properties in animal and clinical studies. The aim of this study was to observe the intraurethral impact of the use of mitomycin-C on scar formation and fibrosis in an experimental rat model of urethral stricture. Materials and Methods: Twelve male Sprague-Dawley rats were used. With the animals under deep anesthesia, an internal urethrotomy was made with a cold knife. After the urethral injury, the rats were grouped randomly as follows: group 1 (control, n=4), group 2 (3 mg/l of mitomycin-C, n=4), and group 3 (5 mg/l of mitomycin-C, n=4). The rats were sacrificed 14 days later to evaluate epithelial proliferation and fibrosis. The penile urethra was removed and histopathologically examined by H&E staining, Masson trichrome staining, and immunohistochemistry with anti-collagen type I antibody. Results: The stained specimens were examined under a light microscope. The extent of fibrosis and re-epithelization after urethral injury was greater in the areas with trauma than in those without. These findings were significantly reduced in the groups treated with mitomycin-C as compared with group 1, but there was no statistical difference between group 2 and group 3. Mitomycin-C treatment also prevented increases in collagen type I, whereas group 1 showed increases in collagen type I and collagen contents at the stricture site. Conclusions: These results suggest that mitomycin-C might inhibit the renewal of the epithelium and the synthesis of collagen secreted by fibroblasts in the affected urethra and then prevent scar formation. This raises the possibility of the use of mitomycin-C to prevent urethral stricture caused by trauma.

골반 골절 환자에 있어서 골절 위치에 따른 하부 요로 손상의 연관성

신대은,문형윤,박성운,노준 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.3

Background and Objectives: Lower urinary tract injuries in pelvic bone fracture can lead to significant complication. We tried to find out the relationship between the fracture location and the bladder, urethra injury in patients who sustaining pelvic bone fracture. We thought that the fracture locations could serves as markers for injury. Materials and Methods: A clinical study was made on 43 patients who sustaining pelvic bone fracture with bladder or urethra injury in the Chosun University hospital during the period of 5 years from January, 2002 to December 2006. Diagnosis was done by retrograde cystography, retrograde urethragraphy, abdomenpelvic computed tomography. Results: Of the 43 patients reviewed, 27 patients had intraperitoneal type bladder rupture, and 12 patients had extra-peritoneal type bladder rupture. 9 patients had urethral rupture. Intraperitoneal type bladder rupture cases are most commonly associated with infrapubic ramus, suprapubic ramus and acetabulum. but the statistically significance is abscent (p= 0.642). Also extraperitoneal type bladder rupture cases are most commonly associated with infrapubic ramus, suprapubic ramus and acetabulum. but the statistically significance is abscent (p= 0.548). And all type bladder rupture cases are most commonly associated with infrapubic ramus, suprapubic ramus and acetabulum. but the statistically significance is abscent (p= 0.775). And urethral rupture cases are most commonly associated with infrapubic ramus, suprapubic ramus and acetabulum but the statistically significance is abscent (p= 0.528). Lastly, Combineded bladder rupture and urethral rupture cases are most commonly associated with infrapubic ramus, llium, Symphysis pubis. But the statistically significance is abscent (p= 0.462). Conclusions: In our study when there is a pelvic bone fracture, the fractured site of pelvic bone have no statistically association with lower urinary tract injury. But most common pelvic bone fracture site of all lower urinary tract injury cases is infrapubic ramus. Therefore, if infrapubic ramus fracture is found in the condition of pelvic bone fracture, it is highly likely to have lower urinary tract injury. More careful observation and diagnosis is necessary for this case.

음낭외상시 고환파열에 대한 초음파 검사의 진단적 유용성

김경영 · 한동준 · 신대은 · 노 준 · 문형윤 · 임동훈 · 김철성 · 박성운 조선대학교 의학연구원 2007 The Medical Journal of Chosun University Vol.32 No.3

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Primary Renal Carcinoid Tumor

서해평,신대은,임동훈,문형윤,김철성 대한비뇨의학회 2009 Investigative and Clinical Urology Vol.50 No.3

Carcinoid tumors are low-grade malignant tumors arising from neuroendocrine cells. Primary renal carcinoid tumors are extremely rare, and only 56 cases have been reported in the literature. Because of the rarity of the lesion, its histogenesis and prognosis are unclear. Here we report a case of a primary renal carcinoid tumor in a 51-year-old man that was found incidentally in a medical examination and was treated by transperitoneal radical nephrectomy.

Squamous Cell Carcinoma of an Ileal Neobladder - A Case Report -

홍란,신대은,문형윤,기근홍,최동열 대한병리학회 2009 Journal of Pathology and Translational Medicine Vol.43 No.5

Bladder reconstruction using bowel segments, especially the ileum, has become a realistic option for urinary diversion. There is only one prior case of squamous cell carcinoma of the ileal neobladder that has been reported in the clinical literature. Here we report a patient with a spectrum of squamous cell lesions, including squamous cell carcinoma, sarcomatoid carcinoma, squamous papilloma and squamous metaplasia that developed in the ileal neobladder. A 46-year-old woman underwent a hysterectomy, cystectomy and ileocystoplasty for tuberculosis 25 years previously complained of urinary frequency and gross hematuria for one week. A pelvic CT revealed a 6.3 cm mass in the neobladder. The histopathological examination showed an 11×8 ㎝ polypoid fragile mass with a microscopically well-differentiated squamous cell carcinoma, squamous papilloma and non-tumor squamous metaplasia.

음낭외상시 고환파열에 대한 초음파 검사의 진단적 유용성

김경영,한동준,신대은,노준,문형윤,임동훈,김철성,박성운 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.3

Purpose: We determined the use of scrotal ultrasonography in the initial diagnosis and management of testicular injuries due to blunt scrotal trauma. Mateiials and Methods: We reviewed 54 patients of scrotal trauma in the last 8 years. Forty patients were evaluated by ultrasonography preoperatively. A heterogeneous echo pattern of the testicular parenchyma with loss of oval shape, contour definition was the basis for diagnosis of testicular rupture. Among them, 33 cases underwent scrotal exploration and 7 cases were managed conservatively. The remaining 14 patients were evaluated only by physical examination, 8 patients of them were explored immediately because of suspicious testicular mpture. The accuracy of ultrasonography were determined by comparing radiographic interpretations with intraoperative findings. Results: Among the 33 patients who were evaluated by ultrasonography and explored immediately, there were 26 suspected testicular ruptures on ultrasonography. Surgical exploration revealed testicular rupture in 23 and simple hematoma in 10. The testicular rupture was correctly diagnosed by ultrasonography in the 22 cases, and there were 4 false-positive and 1 false-negative diagnoses of testicular rupture. The sensivity and specificity for the diagnosis of testicular rupture are 96% and 60%, and the positive and negative predictive values are 85% and 86%, respectively, Conclusions: Scrotal ultrasonography, with the main radiographic finding of a heterogeneous echo pattern of the testicular parenchyma with loss of oval shape, contour definition, is highly sensitive on the diagnosis of testicular rupture. And it increases the salvage rate for testicular rupture with preservation of the testicular function and prevents delayed complications of chronic pain, atrophy and orchiectomy associated with missed testicular rupture.

기술개발형 중소기업의 IP 활용이 기업성과에 미치는 영향 – 균형성과평가제도를 중심으로

전주언,김미리,김희택,신대은 경남대학교 산업경영연구소 2020 지역산업연구 Vol.43 No.2

This study analyzed the relationship between utilizations of intellectual property and balancedscorecard performance of innovative small-medium sized business. The results of this study are asfollows: First, the utilizations of intellectual property of firms have a positive effect on innovation &learning and internal business perspective. Second, the innovation & learning affects customersatisfaction significantly, wheres it affects financial perspective performance non significantly. Third,the internal business perspective affects customer satisfaction significantly, wheres it affects financialperspective performance non significantly. Finally, the customer satisfaction has a positive effect onfinancial perspective performance. As a result of theses empirical analysis, it was found that theutilization of intellectual property rights affects the business performance of innovative small-mediumsized company, The findings of this research contribute to provide practical implications on the IPmanagement. In the process of this study, we had the following limitations, and suggest ways toraise the level of research in the future. 기업가치를 평가하는 과정에서 무형자산으로 간주되는 IP(intellectual property, 지적재산) 활용의 중요성이 높아지고 있는 추세다. 한정된 자원으로 경쟁해야 하는 기술개발형 중소기업은 IP를전략적으로 활용해야 경쟁우위를 확보할 수 있다. 이렇듯 IP 활용의 중요성이 높음에도 불구하고IP 활용이 기업 성과에 미치는 영향에 대한 연구는 부족했다. 본 연구는 기술개발형 중소기업의IP 활용이 학습과 성장, 내부 프로세스 개선, 고객만족, 그리고 재무성과로 이루어진 BSC(balanced scorecard, 균형성과평가제도)에 미치는 영향을 검증하는 것이 목적이다. 연구결과 IP활용은 학습과 성장을 높이고 내부 프로세스를 개선시켜 고객만족도를 높여 재무성과를 달성하는 것으로 나타났다. IP를 적극적으로 활용하는 기업들은 대부분 사업화 초기 단계에 있는 기술개발형 기업들이다. 이러한 기업들은 수익성과 효율성이 검증되지 않아 IP 활용에 대한 회의론이부각되기도 했으나 본 연구결과를 통해 기술개발형 중소기업들의 IP 활용 매뉴얼을 제시할 수있다는 점에서 의미가 있다. 이상의 논의에 근거해 학술적, 실무적 시사점을 제안했다.