발암제(發癌劑) 3-Methylcholanthrene 투여(投與) 마우스에 대(對)한 면역생물학적(免疫生物學的) 연구(硏究): I. 족척종창반응(足蹠腫脹反應) 및 혈중항체가(血中抗體價)

송희종,김종면,Song, Hee-jong,Kim, Jong-myeon 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.1

Experiments were performed on mice to investigate the effects of a polycyclic aromatic hydrocarbon, 3-methylcholanthrene (MCA) on Arthus reaction, delayed-type hypersensitivity (DTH) and antibody production to sheep red blood cells (SRBC). Mice were sensitized iv with 0.1ml of 1% SRBC suspension were treated with a single ip injection of olive oil alone or with different doses of MCA in oil (0.5~50mg/Kg) at various time before (-) or after (+) sensitization (day 0) and were challenged at 4 days after SRBC. Arthus reaction was measured at 3 hours after challenge and other responses at 24 hours. Treatment with MCA inhibited Arthus reaction and DTH to SRBC, measured by footpad swelling reaction, and this immunosuppressing effect was dependent on the dose and time of MCA treatment in relation to SRBC sensitization. Humoral immune responses as measured by serum hemagglutinin-and hemolysin-titers to SRBC were significantly depressed when MCA was injected before or at the same time of sensitization. However, the response was slightly depressed when injected after SRBC. These results indicate that MCA suppress the function of the cells involved in immune responses.

한우 림프구에 대한 단일항체 생산 2. E-rosette형성 림프구에 대한 단일항체

송희종,운재호,김태중,Song, Hee-jong,Woon, Jae-ho,Kim, Tae-joong 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4

A monoclonal antibody(MAb), KCT-23ER, with specificity for E-rosetted T cells of Korean native cattle was prepared by cell hybridization of myeloma P3/NS-1/1-Ag-4-1 and spleen cells from BALB/c mice hyperimmunized with E-rosetted lymphocytes. The isotype of KCT-23ER to T lymphocytes was mouse $IgG_{2b}$. KCT-23ER was reacted with 53.6% to peripheral blood lymphocytes and with 67.8% to nylon wool nonadherent blood lymphocytes. And it was reacted with 72.2%, 59.2% and 35.3% to thymocytes, prescapular lymph node cells and splenocytes, respectively. Immunocytological reactive rates to E-rosetted and non-E-rosetted cells were 72.5% and 22.4%, respectively. These results indicated that KCT-23ER reacted to E-rosetted cells was one of the MAb for investigate of $CD_2$ receptor positive cell subset in the Korean native cattle.

한우 림프구에 대한 단일항체 생산 1. T 림프구에 대한 단일항체

송희종,운재호,김태중,채효석,허부홍,최민순,Song, Hee-jong,Woon, Jae-ho,Kim, Tae-joong,Chai, Hyo-seok,Hur, Boo-hong,Choi, Min-soon 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4

This study was undertaken to develop the monoclonal antibody(MAb) for lymphocytes of Korean native cattle by the cell hybridization of myeloma P3/NS-1/ 1-Ag-4-1 and spleen cells from BALB/c mice hyperimmunized with nylon wool column eluted peripheral T lymphocytes of Korean native cattle. The isotype of MAb KCT-14 against T lymphocyte was mouse $IgG_1$. KCT-14 positivity of mononuclear cells(MNC) from peripheral blood lymphocytes, nylon wool nonadherent and adherent-lymphocytes was 41.7%, 58.4% and 22.6%, respectively. And that of mesenteric lymph node-, spleen and thymus-MNC was 43.3%, 40.2% and 33.6%, respectively. Immunoperoxidase staining of frozen tissue sections showed that the MAb positive cells were located in the medulla of the thymus and in the paracortical area and the mantle zone of the germinal center in the lymph nodes. These results indicated that KCT-14 was one of the MAb for investigate of T lymphocyte subpopulations in the Korean native cattle.

발암제(發癌劑) 3-Methylcholanthrene 투여(投與)마우스에 대(對)한 면역생물학적(免疫生物學的) 연구(硏究) : II. 비장세포(脾臟細胞)의 Rosette형성능(形成能) 및 NK세포(細胞)의 활성(活性)

송희종,김상호,김종면,Song, Hee-jong,Kim, Sang-ho,Kim, Jong-myeon 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.1

The present study was undertaken to evaluate rosette formation and NK activity of splenic lymphocytes in 3-methylcholanthrene (MCA) treated mice. Mice were sensitized iv with 0.1ml of 1% sheep red blood cell (SRBC) suspension were treated with a single ip injection of olive oil alone or with different doses of MCA in oil at various time before or after sensitization, and were challenged at 4 days after SRBC. Rosette formation and NK activity of splenic lymphocytes were measured at 24 hours after challenge. Erythrocyte(E) rosette formation of splenic lymphocytes was significantly depressed in mouse treated with large dose of MCA (5~50mg) regardless of injecting time. But, there was no difference in the response between the treated with small dose of MCA (0.5mg). Whereas erythrocyte-antibody(EA) rosette or erythrocyte-antibody-complement(EAC) rosette forming cells were significantly depressed by MCA. Under small dose of MCA (0.5mg), any difference of NK activity was not observed in all course of injecting time. But, under large dose of MCA, the activity was markedly inhibited to about half the values seen in control and this suppression was transient, resulting that the normal level was reached again 19 days after MCA. These results, which conform with the predictions of immunosuppression hypothesis, suggest that MCA inhibits immunological responses including NK activity and thereby allows the outgrowth of antigenic neoplastic cells.

칠면조와 육계에서 청색증 발생기전에 관한 고찰

송희종 ( Hee Jong Song ),이명우 ( Myung Woo Lee ),류경선 ( Kyeong Sun Ryu ),장형관 ( Hyung Kwan Jang ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.1

Deep pectoral myopathy(DPM), also known as Oregon muscle disease or green muscle disease, was first described in 1968 by Dickinson et al as "degenerative myopathy" in turkeys. Even though this condition was first recognized in adult meat-type turkey and chicken breeders, it is becoming more and more common in meat-type growing birds. DPM occurs exclusively in birds that have been specially selected for breast muscle development. It is generally recognized that DPM is an ischemic necrosis that develops in the deep pectoral muscle(supracoracoideus or pectoralis minor muscle) mainly because this muscle is surrounded by inelastic fascia and the sternum, which do not allow the muscle mass to swell in response to the physiological changes occurring when muscle are exercised, as in wing flapping. The lesion does not impair the general health of birds and is generally found during cut-up and deboning, moreover, it can be both unilateral or bilateral, affecting just one or both pectoralis minor muscle, respectively. No public health significance is associated to DPM, but it is aesthetically undesirable. The fillet should be removed, whereas the rest of the carcass is still fit for human consumption. How-ever, the required trimming operations determine the downgrading of the products and produce an economic loss for the industry, especially because it affects the more valuable part of the carcass. The incidence of DPM increases with market weight in broilers, with more cases reported in higher-yielding strains and in males. Increased bird activity(flock nervousness, flightiness, struggle, and wing flapping) induced by factors such as feed or water outages, lighting programs and intensity, human activity, and excessive noises in and around chicken houses should be looked at as a trigger for the development of DPM in broiler. However, most of the studies conducted to evaluate the incidence of DPM in poultry are concerned with parental commercial breeding stocks under experimental conditions(Bianchi et al. 2006. Poult Sci 85:1843-1846). There is a possible genetic relationship between the selection for large-breasted birds and this condition. Management procedures that discourage excessive wing flapping would reduce the incidence (Jordan and Pattison. 1998. Poultry diseases. 398-399).

파라믹소바이러스 -복제의 단계-

송희종 ( Hee Jong Song ),어성국 ( Seong Kug Eo ),조정곤 ( Jeong Gon Cho ),채효석 ( Hyo Seok Chai ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3

파라믹소바이러스 -분류, 구조, 게놈 및 코드화된 단백질-

송희종 ( Hee Jong Song ),어성국 ( Seong Kug Eo ),채효석 ( Hyo Seok Chai ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.2

가축에서 대장균 감염증

송희종 ( Hee Jong Song ),채효석 ( Hyo Seok Chai ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.4

Escherichia coli is recovered from a wide variety of infections in many animals species. It may be a primary or secondary agent. Nursing and young animals are particularly susceptible, and urinary tract infections are frequent. The various serotypes of E coli are intestinal inhabitants of animals including humans and probably infect most mammals and birds ; therefore, they have a cosmopolitan distribution. Colibacillosis refers to any localized or systemic infection caused entirely or partly by E coli. Collibacillosis in mammals is most often a primary enteric disease, whereas collibacillosis in poultry is typically a secondary located or systemic disease occurring when host defenses have been impaired or overwhelmed. Other opportunistic bacteria, which can be identified by culture, may play a similar role to that of E coli in secondary infections. Collectively, infections caused by E coli are responsible for significant economic losses to the animal performance. From the standpoint of pathogenic mechanisms and diseases, four major categories of E coli are recognized enterotoxigenic(ETEC), enteropathogenic (EPEC), enteroinvasive(EIEC), and enterohemorrhagic(EHEC). In addition, two less-well-defined E coli categories are recognized in animals and humans : enteroaggregative and cytotoxin necrotizing factor-positive. The aforementioned categories are represented by different serotypes. Certain serotypes show a host preference and are encountered more frequently in some disease syndromes. Of the four major categories, ETEC is the most common cause of diarrhea in calves, lambs, and pigs. Strains in the other categories cause the less-common diarrhea and other disease syndromes. Enterotoxins and pilus antigens are the two most prominent virulence factors thus far identified for ETEC. Two enterotoxins, one heat-stable(ST) and one heat-labile(LT), are produced by enterotoxigenic strains of E coli not all culture produce both of these plasmid-based enterotoxins.

Rat에 sulfamethazine sodium 경구투여 후 혈청 및 실질장기내 sulfamethazine의 잔류량 추이에 관한 연구

도재철 ( Jae Cheul Do ),이영미 ( Young Mi Lee ),조민희 ( Min Hee Cho ),신상희 ( Sang Hee Shin ),박희주 ( Hee Ju Park ),송희종 ( Hee Jong Song ),정종식 ( Jong Sik Jyeong ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.4

In order to know the depletive changes of sulfamethazine residues in serum and practical organs of rats orally administered with sulfamethazine sodium(SMS), the concentration of sulfamethazine was measured in serum and tissue(kidney, liver, spleen, testis, and skeletal muscle) of rats with using high perfonnance liquid chromatography(HPLC). SMS was orally administrated to sprague-dawley male rats(body weight, 200 -300g) with using sonde at the rate of 2oing/100g body weight(recommended therapeutic dose) on once a day for 3 days. There were investigated the depletive changes of the sulfamethazine in serum, kidney, liver, spleen, testis and skeletal muscle of rat at the time 8 hours, 1st, 2nd, 3rd, 4th, 5th and 6th day after administration SMS, respectively. The results obtained were summarized as follows; 1. After oral administration of the SMS, the mean concentrations of sulfamethazine in serum according to the time lapsed were showed 215.53 ± 42.99ppm at the 8 hours after withdrawal of medicated sulfamethazine. And gradually according to the time lapsed, the concentrations of sulfamethazine residues in serum were significantly (p<.05) decreased 25.87 ± 5.l8ppm at 1st day, 2.30 ± 0.6lppm at 3rd day and 0.11 ± 0.02ppm at 6th day respectively. 2. The mean concentrations of sulfamethazine in kidney, liver, spleen, muscle and testis according to the time lapsed after administration SMS were showed 83.82 ± 12.16, 81.77 ± 12.88, 36.96 ± 5.35, 35.96 ± 1.39 and 27.89 ± 1.92 ppm at the 8 hours, respectively. And gradually according to the time lapsed, the concentrations of sulfamethazine residues in the each of samples were significantly(p<.05) decreased such as 7.15 ± 0.26, 5.62 ± 0.72, 2.43 ± 0.29, 1.99 ± 0.14 and 3.11 ± 0.48 ppm at 1st day, 0.52 ± 0.04, 1.32 ± 0.22, 0.13 ± 0.03, 0.15 ± 0.06 and 0.26 ± 0.llppm at 3rd day, and 0.03 ± 0.01, 0.11 ± 0.03, 0.02 ± 0.01, 0.009 ± 0.001 and 0.02 ± 0.01 ppm at 6th day, respectively. 3. After oral administration of the SMS to rats, the residual concentrations of sulfamethazine in skeletal muscle were significantly (p<.05) decreased 35.96 ± 1.39 to 0.009 ± 0.00lppm between 8 hours and 6th day, respectively. From the 4th day, the residual concentrations of sulfamethazine were showed 0.10 ± 0.04 ppm below 0.1 ppm at the permitted limit concentration of muscle in Korea. In conclusion, this study could be suggested the relationship between administrated period, doses of sulfonamides and residual aspects of serum and practical organs, and the importance of observing ceasing period of antibiotic drugs before forwarding livestocks to slaughter.

Picibanil이 면양적혈구(緬羊赤血球) 감작(感作)마우스의 면역반응(免疫反應)에 미치는 영향(影響)

채효석,송희종,Chai, Hyo-seok,Song, Hee-jong 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.1

This experiment was performed on mice to investigate the effects of an immunopotentiator, picibanil(PC), on the immune responses such as phagocytic activity of reticuloen-dothelial(RE) system, E rosette formation rate of splenic lmphocytes and morphological changes of lymph node tissue. Groups of mice were treated with a single(1KE/kg BW) or sequential(0.1, 0.25 and 0.5KE/kg BW for successive 3 days) intravenous injections of PC. PC treated and untreated control mice were sensitized with 50% sheep erythrocyte suspension(0.2ml/mouse) at 1, 3, 5, 7 and 10 days after PC treatment. Functional and morphological examinations were carried out 5 days after sensitization. The following results were obtained: The phagocytic activity of RE system and the weight of liver and spleen were increased significantly at 3rd, 5th and 7th day. The peripheral polymorphonuclear leukocyte and percent of lymphocyte and monocyte were slightly increased. The rates of E rosette formation of splenic lymphotytes, sequential PC treated groups were more increased at 3rd and 5th day in sequential PC treated groups than in single treated groups. Thereafter it returned gradually to the control level by the time of 10th day. Microscopically primary lymph follicles with indistinct germinal center (GC) were partially disrupted and the parafollicular areas were consisted of the pyroninophilic cells in control group. In PC treated group, the parafollicular areas were markedly proliferated and developments of secondary lymph follicles with enlarged and prominent GC were more pronounced in the sequential injected groups compared to single injected groups. These results indicate that PC affected not only parafollicular area of the T-cell area, but also GC of the B-cell area. It suggests that PC may potentiate both cell mediated immunity and humoral immunity.