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송재경,Bimala Subba,Madan Kumar Kharel,Hei Chan Lee,류광경,김병기 한국분자세포생물학회 2005 Molecules and cells Vol.20 No.1
A cluster of genes for ribostamycin (Rbm) biosynthesis was isolated from Streptomyces ribosidificus ATCC 21294. Sequencing of 31.892 kb of the genomic DNA of S. ribosidificus revealed 26 open reading frames (ORFs) encoding putative Rbm biosynthetic genes as well as resistance and other genes. One of ten putative Rbm biosynthetic genes, rbmA, was expressed in S. lividans TK24, and shown to encode 2-deoxy-scyllo-inosose (DOI) synthase. Acetylation of various aminoglycosideaminocyclitol (AmAcs) by RbmI confirmed it to be an aminoglycoside 3-N-acetyltransferase. Comparison of the genetic control of ribostamycin and butirosin biosynthesis pointed to a common biosynthetic route for these compounds, despite the considerable differences between them in genetic organization.
송재경,이기헌,박화연,현준영,오승원,배우경,한종수,정세영,엄유진,이가혜,양지혜 대한가정의학회 2017 Korean Journal of Family Medicine Vol.38 No.1
Background: The efficacy of two artificial tears, carboxymethylcellulose (CMC) and hyaluronate (HA), was com-pared in the treatment of patients with dry eye disease.Methods: We conducted a systematic review and meta-analysis on randomized controlled trials in the PubMed, Embase, Cochrane Library, and ClinicalTrials.gov databases. The efficacy was compared in terms of the mean change from baseline in tear break-up time. The meta-analysis was conducted using both random and fixed effect models. The quality of the selected studies was assessed for risk of bias.Results: Five studies were included involving 251 participants. Random effect model meta-analysis showed no sig-nificant difference between CMC and HA in treating dry eye disease (pooled standardized mean difference [SMD]=-0.452; 95% confidence interval [CI], -0.911 to 0.007; P=0.053). In contrast, fixed effect model meta-analysis revealed significant improvements in the CMC group when compared to the HA group (pooled SMD=-0.334; 95% CI, -0.588 to -0.081; P=0.010).Conclusion: The efficacy of CMC appeared to be better than that of HA in treating dry eye disease, although meta-analysis results were not statistically significant. Further research is needed to better elucidate the difference in effi-cacy between CMC and HA in treating dry eye disease.
방위사업 계약에 있어 부당이득금의 합리적 산정방법 연구
송재경,심상렬 한국방위산업학회 2018 韓國防衛産業學會誌 Vol.25 No.4
According to the regulations of ‘Special Law for the Defense Industry’ revised in 1999, Korean government have implemented the unjust profit redemption system in defense program contract. However, conflicts on the estimation of unjust profit or the scope of redemption frequently arise among stakeholders, and many conflicts grow into costly litigations. This study aims to provide a proper estimation method of unjust profit and to specify a model which reasonably reflects the characteristics of defense program, particularly focusing on the incorrect estimation of costs or the fabricated information in government contract. This study is expected to suppress unnecessary conflicts and contribute to improving the efficiency of defense program. 1999년 개정된 「방위산업에 관한 특별조치법」에서 처음 규정된 바에 따라 정부는 방위사업계약을 체결하는 과정에서 계약상대자가 부정한 내용의 원가계산 자료를 정부에 제출하여 부당이득을 얻은 때에 환수하는 부당이득금 환수 제도를 시행하고 있다. 그러나 이러한 부당이득금 산정방법, 즉 환수범위에 대하여 계약당사자 간 논란이 지속되고, 소송으로 이어지는 사례가 빈번하게 발생하고 있다. 이에 본 연구에서는 부당이득에 대한 법적 성격을 살펴보고, 방위사업 현실에 맞는 부당이득금 산정방법을 살펴보았다. 본 연구는 계약당사자 간 부당이득에 대한 분쟁 발생을 억제하고 나아가 원활한 방위사업수행에 기여하고자 한다.
송재경,김도현,변영철,이상준 제주대학교 공과대학 첨단기술연구소 2004 尖端技術硏究所論文集 Vol.15 No.2
Web Service is a new model based on web to offer dynamic information for a user's request. Currently, the web service discovery mechanism is not efficient because it does not support automatic discovery based on the semantics or capabilities of a web service. The language for semantic web service, such as OWL-S, is being developed to express the capability of a web service semantically. Such semantics can support the more flexible discovery of web services. Web service discovery occurs when the provider's description matches to that of requester. But the matching can fail when the ontologies of service description differ from that of the requester although the requester wants that Web Service. In distributed environment service providers develope their semantic web services independently and tend to use their ontologies to describe web services. So a new approach must be required to avoid service discovery failure. In this paper, a method of annotating the query of requester and discovering semantic web services without service discovery failure is proposed.
송재경,Madan K. Kharel,Devi B. Basnet,Hei Chan Lee,류광경,Young Ho Moon,Jae-Jong Kim,Jin Suk Woo 한국분자세포생물학회 2004 Molecules and cells Vol.18 No.1
The organization of the 2-deoxystreptamine (DOS) biosynthetic gene cluster of Micromonospora echinospora has been determined. Sequencing of a 14.04 kb-region revealed twelve open reading frames (ORFs): four putative DOS biosynthetic genes (gtmA, B, C, and D), five amino sugars biosynthetic genes (gtmE, G, H, I, and gacB), two aminoglycoside resistance genes (gtmF and J) as well as a hypothetical ORF (gacA). One of the putative DOS biosynthetic genes, gtmA, was expressed in Escherichia coli, and the purified protein was shown to convert glucose-6-phosphate (G-6-P) to 2-deoxyscyllo- inosose (DOI), a key step in DOS biosynthesis. In addition gtmJ was expressed in Streptomyces lividans and shown to confer gentamicin resistance. Thus gtmA and gtmJ are implicated in the biosynthesis of gentamicin and in resistance to it, espectively.
송재경,이희찬,Trinh Viet Hung,Kenji Ishida,Niranjan Parajuli,Kwangkyoung Liou 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.2
We constructed four recombinant plasmids to enhance the production of clavulanic acid (CA) in Streptomyces clavuligerus NRRL3585: (1) pIBRHL1, which includes ccaR, a pathway-specific regulatory gene involved in cephamycin C and CA biosynthesis; (2) pIBRHL2, containing claR, again a regulatory gene, which controls the late steps of CA biosynthesis; (3) pGIBR containing afsR-p, a global regulatory gene from Streptomyces peucetius; and (4) pKS, which harbors all of the genes (ccaR/claR/afsR-p). The plasmids were expressed in S. clavuligerus NRRL3585 along with the ermE* promoter. All of them enhanced the production of CA; 2.5-fold overproduction for pIBRHL1, 1.5-fold for pIBRHL2, 1.6-fold for pGIBR, and 1.5-fold for pKS compared to the wild type.