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만성폐쇄성폐질환의 급성악화와 회복기에 유도 객담 내 Nuclear Factor-κB(NF-κB)의 활성도 와 IL-6, IL-8 및 TNF-α의 농도 변화
송소향,김영균,김치홍,권순석,김관형,문화식,송정섭,박성학 대한결핵및호흡기학회 2005 Tuberculosis and Respiratory Diseases Vol.58 No.2
Background : Exacerbations of chronic obstructive pulmonary disease (COPD) are thought to be associated with increased airway inflammation, and the NF-κB is known to be an indicator of cellular activation and of inflammatory mediator production. This study was undertaken to investigate the change of cytokine characteristics and NF-kB activity in induced sputum of COPD patients during exacerbation and recovery of the disease.Methods : Sputum induction was performed in 37 patients with COPD during exacerbation and during recovery and in 15 healthy subjects. Cell counts, levels of IL-6, IL-8 and TNF-α in induced sputum and NF-kB activity in macrophage of induced sputum were measured.Results : Patients with COPD showed significantly increased levels of IL-6, IL-8 and TNF-α(p<0.01) and increased NF-kB activity in induced sputum(p<0.05) as compared with control subjects. Level of IL-8 during exacerbation of COPD decreased significantly during recovery(p<0.05). NF-kB activity and levels of IL-6 and TNF-α tended to be decreased during recovery, but not siginificantly. Conclusion : Activation of NF-kB and increased levels of IL-6, IL-8 and TNF-α were thought to be associated with pathogenesis and exacerbations of COPD. (Tuberc Respir Dis 2005; 58:152-159)
Micro-Bumintest 및 방사면역 측정법을 이용한 미세단백뇨의 진단 및 비교
송소향,윤석중,윤건호,강무일,홍관수,차봉연,이광우,손호영,강성구 대한내과학회 1992 대한내과학회지 Vol.42 No.1
이상과 같이 미세단백뇨의 측정에 있어서 소변으로의 크레아티닌 배설양을 고려하여 보정한다면 spot urine만으로도 일정시간 동안 모든 소변이나 24시간 소변에서의 미세단백뇨량과 유사한 결과를 얻을 수 있을 것 같으며, spot urine을 대상으로 간편하고 신속하게 Micro-Bumintest를 실시하여 미세단백뇨 유무를 판단함으로써 당뇨병성 신증의 조기발견, 예방 및 치료에 도움을 줄 것으로 생각된다. Microalbuminuria may be a useful predictor of nephropathy in diabetes. Moreover, recent interventional studies have demonstrated that intensive insulin regimens may correct the increased albumin excretion in diabetes. Studies of microalbuminuria have relied on either spot urine samples, timed collection, or 24 hr urine collections. Although the validity of single-void urine samples in quantitating gross proteinuria has been established, confirmation of the utility of a single-void specimen in estimating microalbuminuria has been performed. We conducted a study to determine whether singlevoid urine samples (corrected for creatinine) can be used to estimate 24 hr excretion and to evaluate the performance of Micro-Bumintest in screening for microalbuminuria in diabetic patients. Also, the results of the Micro-Bumintest were compared with quantitative RIA determinations of the urinary microalbumin concentration. The results were as follows: 1) The age, duration of the diabetes, degree of diabetic control measured by HbA_(IC), serum creatinine and microalbuminuria by radioimmunoassay are shown in Table 1. 2) The overall correlation of spot urine sample results expressed as microgram albumin per milligram creatinine (r=0.54, p=0.0001) and with 24 hr urine microalbuminuria expressed as microgram per milligram (r=0.55, p=0.0001) was good (Fig. 2, 3). 3) When 20 mg/min (24 hr albumin excretion) was used as the upper limit of normal, the sensitivity and specificity of Micro-Bumintest in 24 hr urine specimen were 93.8% and 64.7%, respectively (Table 2). 4) BMI, serum creatinine, and microalbuminuria were significantly associated between positive and negative Micro-Bumintest results (p<0.01, p<0.05, p<0.01) (Table 3). In summary, spot urine specimens are useful in estimating the excretion in place of 24 hr urine collections, and the Micro-Bumintest is a reasonable screening method for microalbuminuria.
기관지천식 마우스에서 기저막하 섬유화와 관련된 인자들: TGF-β1및 IL-17에 대한 CpG-oligodeoxynucleotides의 영향
송소향 ( So Hyang Song ),김치홍 ( Chi Hong Kim ),문화식 ( Hwa Sik Moon ),송정섭 ( Jeong Sup Song ),박성학 ( Sung Hak Park ) 대한천식알레르기학회 2006 천식 및 알레르기 Vol.26 No.2
Background: Important features of airway remodeling in asthma include the formation of subepithelial fibrosis and increased deposition of collagen. Profibrotic cytokines such as TGF-β1 and IL-17 are involved in the formation of subepithelial fibrosis and are increased in patients with asthma, particularly in these severe disease. Previous reports have represented that administration of CpG-oligodeoxynu-cleotides (CpG-ODN) before allergen sensitization or challenge inhibits chronic inflammation and remodeling of airway in mice with chronic asthma, but the effect of CpG-ODN on TGF-β1 and IL-17 have not been evaluated. Objective: To evaluate the effect of CpG-ODN on the expression of these profibrotic cytokines and on collagen deposition, this study was performed in mice with chronic asthma. Method: Mice were subjected to repetitive ovalbumin (OVA) challenge for 2 months. CpG-ODN was administered intraperitoneally for sensitization. After the final challenge, airway hyperresponsiveness, peribronchial fibrosis, and levels of lung hydroxyproline were assessed. Levels of TGF-β1 and IL-17 in bronchoaveolar lavage (BAL) were assessed by ELISA and the number of TGF-β1+cells and IL-17+cells in peribronchial area by immumohistochemistry. Result: CpG-ODN significantly reduced airway hyperresponsiveness, eosinophil infiltration, and peribronchial fibrosis. Levels of TGF-β1 in BAL fluid (353±94 vs 270±66 pg/mL, OVA vs OVA+CpG, P<0.05) and peribronchial TGF-β1+cells (46±10 vs 27±5 TGF-β1+ cells/bronchus, OVA vs OVA+CpG, P<0.01) were significantly reduced in mice treated with CpG-ODN. Levels of IL-17 in BAL fluid (62±30 vs 34±10 pg/mL, OVA vs OVA+CpG, P=0.074) were not significantly reduced, but peribronchial IL-17+cells (27±7 vs 7.5±5.3 IL-17+ cells/bronchus, OVA vs OVA+CpG, P<0.01) and levels of lung hydroxyproline (13.1±5.3 vs 9.1±2.71ug/mL, OVA vs OVA+CpG, P<0.01) were significantly reduced in mice treated with CpG-ODN. Conclusion: CpG-ODN significantly reduced the levels of allergen-induced peribronchial fibrosis and collagen deposition. The reduction in peribronchial fibrosis and collagen deposition mediated by CpG-ODN might be due to several mechanisms including inhibition of profibrotic cytokines of TGF-β1 and IL-17 and a reduction in the number of peribronchial inflammatory cells expressing TGF-β1. (Korean J Asthma Allergy Clin Immunol 2006;26:136-144)