SFMOG : 초고속 MOG 기반 배경 제거 알고리즘

송석빈,김진헌 한국전기전자학회 2019 전기전자학회논문지 Vol.23 No.4

배경 제거는 동영상에서 변화를 감지하는 컴퓨터 비전 및 이미지 처리의 주요 작업이다. 최상의 성능을 가지는 배경 제거방법은 일반적인 컴퓨팅 환경에서 실시간으로 사용할 수 없을 만큼 계산량이 많다. 제안하는 알고리즘은 널리 사용되는MOG 기반의 배경 제거 알고리즘을 이미지 크기 조정 알고리즘으로 개선했다. 제안된 이미지 크기 조정 알고리즘은 계산량을 대폭 감소시키고 지역 정보를 활용하도록 설계해 카메라 잡음에 강력하다. 제안된 알고리즘의 실험결과는 최신 배경 제거방법에 근접하는 분류능력과 13배 이상 빠른 처리 속도를 가진다. Background subtraction is the major task of computer vision and image processing to detect changes in video. Thebest performing background subtraction is computationally expensive that cannot be used in real time in a typicalcomputing environment. The proposed algorithm improves the background subtraction algorithm of the widely usedMOG with the image resizing algorithm. The proposed image resizing algorithm is designed to drastically reduce theamount of computation and to utilize local information, which is robust against noise such as camera movement. Experimental results of the proposed algorithm have a classification capability that is close to the state of the artbackground subtraction method and the processing speed is more than 10 times faster.

아스팔트 수분저항성 증대를 위한 Nano Seal 표면처리공법의 실내 시험 및 현장시험을 통한 현장적용성 분석

송석빈,최준성,김조순 한국도로학회 2012 한국도로학회 학술대회 발표논문 초록집 Vol.2012 No.03

SFMOG:초고속 MOG 기반 배경 제거 알고리즘

송석빈(Seok-bin Song),김진헌(Jin-Heon Kim) 한국전기전자학회 2019 전기전자학회논문지 Vol.23 No.4

배경 제거는 동영상에서 변화를 감지하는 컴퓨터 비전 및 이미지 처리의 주요 작업이다. 최상의 성능을 가지는 배경 제거 방법은 일반적인 컴퓨팅 환경에서 실시간으로 사용할 수 없을 만큼 계산량이 많다. 제안하는 알고리즘은 널리 사용되는 MOG 기반의 배경 제거 알고리즘을 이미지 크기 조정 알고리즘으로 개선했다. 제안된 이미지 크기 조정 알고리즘은 계산량을 대폭 감소시키고 지역 정보를 활용하도록 설계해 카메라 잡음에 강력하다. 제안된 알고리즘의 실험결과는 최신 배경 제거 방법에 근접하는 분류능력과 13배 이상 빠른 처리 속도를 가진다. Background subtraction is the major task of computer vision and image processing to detect changes in video. The best performing background subtraction is computationally expensive that cannot be used in real time in a typical computing environment. The proposed algorithm improves the background subtraction algorithm of the widely used MOG with the image resizing algorithm. The proposed image resizing algorithm is designed to drastically reduce the amount of computation and to utilize local information, which is robust against noise such as camera movement. Experimental results of the proposed algorithm have a classification capability that is close to the state of the art background subtraction method and the processing speed is more than 10 times faster.

LIM domain과 상호작용 하는 transcription cofactor hTAiL-1의 기능 분석

박경숙,송석빈,김균언 충남대학교 자연과학연구소 1999 忠南科學硏究誌 Vol.26 No.1

The LIM homeodomain (LIM-HD) proteins, which contain two tandem LIM domains followed by a homeodomain, are critical transcription factors for embryonic development. The LIM domain is a conserved cysteine-rich zinc-binding motif and mediate protein-protein interactions. We have isolated two transcription activator interacting with LIM domain (hTAiL-1 and -2) from human brain cDNA library on the basis of its ability to interact with the LIM-HD protein Lh-2 (or Lhx-2). Here we report on the function of the hTAiL-1 protein. Northern blot analysis using human tissue blot revealed that hTAiL-1 is very abundantly expressed in the heart and muscle. In order to confirm that hTAL-1 interacts with LIM domain, β-galactosidase assay using yeast two-hybrid system was firstly employed, followed by an in vitro protein interaction assay and a mammalian two-hybrid assay. The results of these assay demonstrated that hTAiL-1 interact with LIM domain in a very weak mode. In vivo functional data using transfection experiments in SL-2 cells suggested that Sp1 transcription factor may be involved in the interaction of hTAiL-1 with Lh-2. In fact, hTAiL-1 stimulates the promoter activity deriven by Sp1 more efficiently than by Lh-2. In addition, effect of hTAiL-1 can be observed in a series of transfection experiment with differentiated L6 cells. Taken together, these results indicated that hTAiL-1 may mediate specific protein-protein interactions between LIM-HD and additional Sp1 basal transcription factor.

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway

김희정,송석빈,최정민,김경문,조백기,조대호,박현정 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

LIM-homeobox 2 (Lhx-2)에 의한 갑상선자극호르몬 β-subunit 유전자의 전사조절

김기광,송석빈,이진욱,박진수,김용은,이재훈,김균언 충남대학교 생물공학연구소 2002 생물공학연구지 Vol.8 No.2

Thyrotropin (TSH) β is a subunit of TSH, the expression of which is limited to the thyrotrope cells of the anterior pituitary gland. LIM homeodomain factors appear to play a role in expression of the glycoprotein hormone α-subunit gene. Studies demonstrated that LIM homeodomain factor-2 (Lhx2) can bind to a pituitary-specific enhancer element designated as PGBE in the mouse α-subunit gene. Lhx3 (pLIM) can also enhance α-subunit gene expression. However, the mode of Lhx2 action is not yet characterized in the TSH β-subunit gene expression. In this study, deletion analysis was employed to delineate the sequence of the rTSH β-subunit gene which are inolved in Lhx2 regulation. Transient transfection experiment is αTSH and GH_3 cells demonstrated that the -170 / -79 region of the rTSH β-subunit gene, when fused to a luciferase reporter, was sufficient for the activation of this gene by Lhx2. Furthermore, gel mobility shift assay showed that homeodomain of Lhx2 binds to the -170 / -79 region of the rTSH β-subunit promoter. Taken together, these data suggest a model, in which the Lhx2 activates transcription of the TSH β-subunit gene.

사람의 성선자극 호르몬 α-Subunit 유전자와 결합하는 Trans - Acting Factor 의 확인 및 분석

백상기,송석빈,김균언 한국유전학회 1998 Genes & Genomics Vol.20 No.1

The 5' flanking region of the α-subunit gene of human chorionic gonadotropin was tested for the binding with the nuclear extracts of the JAR choriocarcinoma cells. Gel mobility shift assay has demonstrated that two BstNI restriction fragments, 150 bp and 340 bp respectively, have binding activities with the nuclear extracts. The 150 bp fragment was chosen to examine in detail, since several transcription factor binding sites have already been reported within the 340 bp fragment. Band competition assay showed that the binding of 150 bp fragment is highly sequence-specific. The binding activity was further narrowed down to a 55 bp fragment, which was generated by Nsil digestion of the 150 bp fragment. DNasel footprinting assay revealed the binding sites on the nucleotide levels from -511 to -499. The binding sites were again confirmed by a oligonucleotide-directed mutagenesis of the 150 bp fragment followed by a gel mobility shift assay. Functional aspect of this binding was evaluated with the DNA-mediated gene transfer techniques. However, in several approaches tried so far, no effect on the transcription efficiency was observed, implicating a structural role of the binding. In this line, it is worth to note that the binding activity of the 150 bp fragment was observed in every cell or tissue nuclear extracts tested so far.

The Nuclear Orphan Receptor NR4A1 is Involved in the Apoptotic Pathway Induced by LPS and Simvastatin in RAW 264.7 Macrophages

김용찬,송석빈,이상규,박상민,김영상 대한면역학회 2014 Immune Network Vol.14 No.2

Macrophage death plays a role in several physiological and inflammatory pathologies such as sepsis and arthritis. In our previous work, we showed that simvastatin triggers cell death in LPS-activated RAW 264.7 mouse macrophage cells through both caspase-dependent and independent apoptotic pathways. Here, we show that the nuclear orphan receptor NR4A1 is involved in a caspase-independent apoptotic process induced by LPS and simvastatin. Simvastatin-induced NR4A1 expression in RAW 264.7 macrophages and ectopic expression of a dominant-negative mutant form of NR4A1 effectively suppressed both DNA fragmentation and the disruption of mitochondrial membrane potential (MMP) during LPS- and simvastatin-induced apoptosis. Furthermore, apoptosis was accompanied by Bcl-2-associated X protein (Bax) translocation to the mitochondria. Our findings suggest that NR4A1 expression and mitochondrial translocation of Bax are related to simvastatin-induced apoptosis in LPS-activated RAW 264.7 macrophages.

흰쥐 μ(mu)opioid Receptor 유전자의 클로닝 및 발현 조절

설은영,송석빈,김균언 한국유전학회 1998 Genes & Genomics Vol.20 No.3

Chromosomal gene encoding a μ(mu) opioid receptor (μORc) was cloned from a rat genomic library using μORc cDNA as a probe. Sequence analysis of the clone identified a transcription start site, four AP-1 sites, two Oct-1 sites and one CREBP1 site in the immediate 5' flanking region as well as a translation initiation codon ATG in exon I. However, canonical TATA box was not found in the rat μORc promoter region. Northern blot analysis demonstrated that μORc gene is expressed in the hypothalamus-derived GT1-1 cells and pituitary gonadotrope-derived αT3-1 cells but neither in pituitary lactotrope-derived GH₃ cells nor in pituitary thyrotrope-derived αTSH cells. In addition, expression of the gene was induced in the cells treated with cAMP for 24 hrs. A series of deletion mutants of the 5'-flanking region were subcloned into the luciferase reporter vector, and tested for their transcriptional activities by transfecting into αT3-1 cells. The construct containing up to -2967 bp region exhibited maximum transcriptional activity among the tested constructs, whereas the region below -1705 bp conferred significantly low levels of activity. Therefore, it is conceivable that the region between -2967 bp and -1705 bp is responsible for the basal expression of the μORc gene.

Erythroid differentiation regulator 1 (Erdr1) is a new proapototic factor in human keratinocytes

김희정,송석빈,양율희,은영선,조백기,박현정,조대호 대한피부과학회 2011 초록집 Vol.49 No.20