Glycerol-Phosphate Acyltransferase Gene Expression의 호르몬에 의한 조절

손승렬,신동훈 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.5

Glycerol-phosphate acyltransferase와 7.2 kb mRNAs 모두가 간 조직에 가장 많이 존재하였다. Glycerol-phosphate acyltransferase와 7.2 kb mRNA 농도가 단식시킨 생쥐들에게 고농도 탄수화물 사료를 재급식할 경우에 상당히 증가 되었다. 성숙된 3T3-L1 adipocytes에서는 인슐린이 glycerol-phosphate acyltransferase와 7.2 kb mRNA의 농도를 2.6∼3배 증가시켰고, 반면에 dibutyryl cAMP는 mRNA 농도를 각각 50%, 80% 정도 감소시켰다. 이러한 결과들은 glycerol-phosphate acyltransferase와 7.2 kb mRNA에 대하여 인슐린에 의하여는 positive regulation(조절)이 되고 cAMP에 의하여는 negative regulation이 되는 것을 보여준다. Both glycerol-phosphate acyltransferase (GPAT) and 7.2 kb mRNAs were present at the highest level in liver. Glycerol-phosphate acyltransferase and 7.2 kb mRNA levels increased dramatically when fasted mice were refed a high carbohydrate diet. In mature 3T3-L1 adipocytes, insulin increased both glycerol-phosphate acyltransferase and 7.2 kb mRNA levels 2.6 to 3-fold while dibutyryl cAMP decreased mRNA levels by 50% and 80%, respectively. These results indicate positive regulation by insulin and negative regulation by dibutyryl cAMP of both glycerol-phosphate acyltransferase and 7.2 kb mRNA.

Glycerol-phosphate acyltransferase gene expression의 호르몬에 의한 조절

손승렬,신동훈 檀國大學校 基礎科學硏究所 1995 論文集 Vol.6 No.-

형질 전환에 의한 DNA수선 기작에 관한 연구

손승렬,허승재 단국대학교 1998 論文集 Vol.33 No.-

Genetic transformation occurs naturally in a number of groups in bacteria, including Escherchia and Bacillus. The repair hypothesis proposes that a DNA fragment taken up by a cell is used as a template for recombinational repair in the recipient cell’s genome which is damaged. We developed an experimental system for studying DNA repair mechanisms through transformation after UV irradiation. The nature of transformed DNA are homologous chromosomal DNA. plasmid DNA and totally different salmon sperm DNA. Comparison of relative survival rates among control group and DNA transformation groups after UV irradiation revealed that a significantly higher survival rate was observed in chromosomal DNA group than in plasmid DNA group and in salmon sperm DNA group. In conclusion, the increased survival rate was caused by homologous DNA recombinational repair and we propose that this effect on increased survival rate might have been evolved to differentiate bacterial sexes, and then real sex in eukaryotes.

The Structure and Regulation of Histone Genes

Son, Seung-Yeol 한국생화학분자생물학회 1993 생화학분자생물학회 소식 Vol.13 No.2

Biologically Active and C-Amidated HinnavinII-38-Asn Produced from a Trx Fusion Construct in Escherichia coli

강창수,손승렬,방인석 한국미생물학회 2008 The journal of microbiology Vol.46 No.6

The cabbage butterfly (Artogeia rapae) antimicrobial peptide hinnavinII as a member of cecropin family is synthesized as 37 residues in size with an amidated lysine at C-terminus and shows the humoral immune response to a bacterial invasion. In this work, a synthetic gene for hinnavinII-38-Asn (HIN) with an additional amino acid asparagine residue containing amide group at C-terminus was cloned into pET-32a(+) vector to allow expression of HIN as a Trx fusion protein in Escherichia coli strain BL21 (DE3) pLysS. The resulting expression level of the fusion protein Trx-HIN could reach 15~20% of the total cell proteins and more than 70% of the target proteins were in soluble form. The fusion protein could be purified successfully by HiTrap Chelating HP column and a high yield of 15 mg purified fusion protein was obtained from 80 ml E. coli culture. Recombinant HIN was readily obtained by enterokinase cleavage of the fusion protein followed by FPLC chromatography, and 3.18 mg pure active recombinant HIN was obtained from 80 ml culture. The molecular mass of recombinant HIN determined by MALDI-TOF mass spectrometer is 4252.084 Da which matches the theoretical mass (4252.0 Da) of HIN. Comparing the antimicrobial activities of the recombinant hinnavinII with C-amidated terminus to that without an amidated C-terminus, we found that the amide of asparagine at C-terminus of hinnavinII improved its potency on certain microorganism such as E. coli, Enterobacter cloacae, Bacillus megaterium, and Staphylococcus aureus.

국내토양에서 분리한 점액세균의 동정및 특성

김재헌,손승렬 한국미생물학회 2001 미생물학회지 Vol.37 No.4

We isolated a Myxobacteria strain from a soil sample obtained from Mt. Daedoon located in Choongnam, Korea. This strain, ARJ, secreted slime while swarmed on the surface of CT medium. It produced greenish yellow pigment in liquid or solid media, and the swarming edge showed green florescence under U. V. at 366 nm. It formed fruiting bodies when nutrient was exhausted, which is one of the most imkportant characteristics of Myxobacteria. The fruiting bodies did not have a stalk and consisted of naked myxospores when examined under the scanning electron microscope. These traits lead us to believe that this strain is very close to Myxococcus virescens. It showed antimicrobial activity, especially against Gram positive bacteria. Culture filtrate showed the activity but this was not due to protein. The culture filtrate also had proteolytic activity in which at least two enzymes are involved. 충남 대둔산의 토양으로부터 분리한 점액세균을 ARJ라 명명하고 그 특성을 알아보았다. CT 배지에서 swarming을 하며 성장한 이 균주는 slime을 형성하였고, 액체 및 고체배지 상에서 황록색의 색소를 방출하였으며 swarming 부분을 파장이 366 nm인 자외선으로 조사하였을 때 초록색의 형광을 띄었다. 또한 이 균주는 영양분이 고갈되었을 때 fruiting body를 형성하는 것으로 보아 Myxobacteria라고 생각되었으며 주사전자 현미경 관찰 결과 이 균주가 형성한 fruiting body는 stalk이 없었고 naked myxospore를 가지고 있었다. Myxobacteria의 분류에 있어서 가장 중요한 이러한 형태학적 특성들로 볼때 이 균주는 Myxococcus virescens와 가장 유사한 것으로 밝혀졌다. 한편 이 균주는 특히 gram 양성 세균에 대해 antimicrobial activity가 있는 것으로 나타났는데, 여과한 배양 여액에서도 이렇ㄴ activity가 있었지만 비변성 polyacrylamide 전기영동을 하여 본 결과 이 물질이 단백질은 아닌 것으로 밝혀졌다. 또한 이 배양 여액은 상당한 proteolytic activity가 있었으며 최소한 2개의 proteolytic enzyme이 있는 것으로 비변성 polyacrylamide 전기영동을 통하여 밝혀졌다.

팽이버섯으로부터 Lectin 의 정제와 특성

황세영,홍범식,손승렬,김형석 한국농화학회 1999 Applied Biological Chemistry (Appl Biol Chem) Vol.42 No.4

Two Lectins, designated FVL-1 and FVL-2, were isolated and purified from the fruiting bodies of edible mushroom Flammulina veluripes using ammonium sulfate fractionation, ethanol treatment, DEAE-TOYPEARL ion-exchange column chromatography, and TSK-Gel HW-55F column chromatography. Specific activity increased 18 folds for FVL-1 and 7.9 folds for FVL-2 from ethanol treated sample. SDS-PAGE of FVL-1 and FVL-2 gave apparent molecular mass of 10.6 kDa and 37 kDa, respectively. FVL-2 agglutinated all type of human erythrocytes (A, B, AB, and O). However, FVL-1 agglutinated more human erythrocyte type O than type A, B, and AB. The hemagglutination activities of the FVL-1 were effectively inhibited by bovine submaxillary and porcine stomach mucins(BSM and PSM), fetuin, asialofetuin and cations, such as Cu^(2+), Mg^(2+), Ca^(2+), Mn^(2+) and Fe^(2+). However, FVL-2 was not inhibited by any cations. The hemagglutination activities of the two lectins were not inhibited by the sugar, such as lactose, galactose and sugar derivatives. FVL-1 and FVL-2 were stable at pH 5∼11 and pH 4∼7, respectively. FVL-1 was stable below 55℃ and FVL-2 was below 45℃.

Whole-exome sequencing in Tricho-rhino-phalangeal syndrome (TRPS) type I in a Korean family

윤별이,김윤지,손승렬,한규동,박병철 한국유전학회 2017 Genes & Genomics Vol.39 No.4

Tricho-rhino-phalangeal syndrome (TRPS) is a rare autosomal dominant and monogenic disease. Among three types of TRPS, it is known that TRPS type I and type III are caused by deletions or substitutions in the TRPS1 gene, located on chromosome 8 (8q23.3). Although the mutations in TRPS1 gene are responsible for human TRPS, some cases are not detected by the mutations of TRPS1 gene and several cases are presented with different genetic variations. The present case was a sporadic and without TRPS1 mutation. Therefore, we performed whole-exome sequencing (WES) with one patient and his family (father, mother, and brother) and validated novel mutations using PCR and Sanger sequencing. Through family-based WES, we found the two de novo mutations such as ZNF 134 and EXD 3 genes. Through functional effect prediction using disease association Ensembl database, we propose that the de novo mutation of ZNF134 (p.Ser207Arg) could be one of potential candidate genes for causing TRPS and develope the TRPS phenotype in the present case.

PAHs를 분해하는 Sphingomonas sp. K-19의 분리 및 특성

한규동,김성환,손승렬,Kim, Seong-Hwan,Son, Seung-Ryeol 한국미생물학회 2003 미생물학회지 Vol.39 No.4

Polycyclic aromatic hydrocarbon (PAHs)들에 오염된 토양시료를 채취한 후, phenanthrene을 유일한 탄소원과 에너지원으로 배양하여 PAHs를 분해하는 균주 K-19를 분리하였다. 이 균주는 지방산 조성 실험에서 Sphingomonas 속 균주의 특징인 2-hydroxy fatty acid들과 18:1w7를 포함하고 있었으며, 16S rDNA 염기서열을 이용한 계통유전학적 분석결과 Ribosomal Database에서 Sphingomonas CF06과 가장 가까운 유연관계를 보였다. K-19는 phenanthrene 존재 하에서 빠른 성장률과 높은 phenanthrene 분해율로 10일만에 500 ppm의 phenanthrene을 92% 분해하였으며, phenanthrene 이외에 indole, naphthalene 등도 분해하였다. 또한 전 배양 방법을 통하여 phenanthrene 분해능이 더욱 증진됨을 알 수 있었다. 이러한 결과는 우리나라의 유류 오염토양에 Sphingomonas 속의 미생물이 존재하며 이 미생물은 여러 종류의 다중 고리 방향족 화합물을 분해할 수 있는 능력을 가지고 있음을 보여준다. We isolated a bacterium, K-19, which could degrade PAHs (polycyclic aromatic hydrocarbons) from a soil sample contaminated with PAHs. K-19 was cultured under the conditions where phenanthrene was a sole source of carbon and energy.

훈증방제 처리한 참나무시들음병 감염목의 사상균 조사

서동연 ( Dong Yeon Suh ),손승렬 ( Seong Yeol Son ),김성환 ( Seong Hwan Kim ),서상태 ( Sang Tae Seo ),김경희 ( Kyung Hee Kim ),고한규 ( Han Kyu Ko ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.4

한국에서 발병하는 참나무시들음병은 암브로시아 곤충인 Platypus koryoensis에 의해 전반되는 Raffaelea quercusmongolicae 균류에 의해 발생한다. 이 병의 전파를 방지하기 위해 참나무시들음병 감염목을 벌채 후 비닐막을 씌워 훈증하고 벌채장소에 3년 이상 방치시키고 있다. 본 연구는 이런 훈증처리된 감염목에 존재하는 균류에 대한 정보를 얻고자 수행하였다. 천안 태조산 지역 두 곳에서 훈증처리된 감염목을 채집하고 진균을 분리하여 99개 균주를 얻었다. 분리 균주를 형태적 특성과 translation elongation factor 1-alpha 유전자와 ITS rDNA region 염기서열에 의거한 분자적 방법으로 동정한 결과 Hypocrea, Trichoderma, Penicillium 속에 속하는 종들이 확인되었다. 이중 Trichoderma 속에 속하는 종이 주요 균류이었다. 조사결과 병원균인 R. quercus-mongolicae와 매개충인 P. koryoensis는 검출되지 않았다. 본 연구결과는 훈증 처리한 참나무시들음병 감염목이 3년 이상 되면 더 이상의 피해를 유발할 위험요소가 없다는 과학적 증거를 제시한다. Korean oak wilt disease caused by Raffaelea quercus-mongolicae is vectored by the ambrosia beetle Platypus koryoensis. To prevent the spread of the disease, the beetle infested oak tree had been cut into logs, covered with plastic vinyl, fumigated with a pesticide, and stored for three years on the site where the tree was cut. This study was carried out to get information on the fungi colonizing the fumigated oak wood. Wood disk samples collected from the fumigated oak logs at two locations in the Taejo Mountain, Cheonan city, were used for fungal isolation. A total of 99 filamentous fungal isolates were obtained from the wood disk samples. Hypocrea spp., Trichoderma spp. and Penicillium spp. were identified based on morphological characteristics and nucleotide sequence analysis of translation elongation factor 1-alpha gene and ITS rDNA region. Trichoderma was the major fungal group. R. quercus-mongolicae, and P. koryoensis were not detected from the fumigated oak wood. Our work provided evidence that after three years of storage, the fumigated oak wilt-diseased logs should be no longer harmful source of oak wilt disease transmission.