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국내 가금유래 병원성 대장균의 extended-spectrum β-lactamase(ESBL) 특성 조사
성명숙,김진현,조재근,설성용,김기석,Sung, Myung-Suk,Kim, Jin-Hyun,Cho, Jae-Keun,Seol, Sung-Yong,Kim, Ki-Seuk 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.3
This study was conducted to investigate incidence of extended-spectrum ${\beta}$-lactamase (ESBL) producing strains and characteristics of ESBL gene in pathogenic Escherichia coli isolated from poultry during the period from April 2003 to December 2005 in Korea. Among 203 isolates, 4 isolates (3 from broilers and 1 from layer) were confirmed as ESBL producing strains by double disk synergy test, polymerase chain reaction and sequencing for ${\beta}$-lactamase genes. $bla_{CTX-M-15}$ and $bla_{CMY-2}$ were detected in these 4 isolates and were transferred to recipient by conjugation, respectively. Also, these ESBL producing strains were associated with multiple drug resistance. In conclusion, these results exhibit incidence of CTX-M and CMY-2 ${\beta}$-lactamase in pathogenic E coli from poultry in Korea, and clinically important meaning in human. And they also suggest the needs for rapid and broad surveillance to monitor ESBL genes and R plasmid transferring resistant gene in poultry.
도계과정에서의 Salmonella속 균의 오염에 대하여
성명숙,김기석,탁연빈 한국수의공중보건학회 2002 예방수의학회지 Vol.26 No.3
This study was conducted to find out Salmonella contamination during the processing at a chicken slaughterhouse in Gyeongbuk province. Seventy-two strains of Salmonella were isolated from 465 samples consisting scalding water, washing water, chilling water, cecal contents and carcasses. The ratio of isolation from chilling water samples was 30.8%, 26.0% from carcasses, 7.7% from washing water samples, and 3.5% from cecal contents, but no strain was isolated from scalding water samples. Among Salmonella isolated, S. enteritidis(17 strains), S. newport(12 strains), S. blockley(11 strains), S. virchow(9 strains) were frequent serovars. S. senftenberg 5 strains, S. ochi 4 strains, S. schwarzengrund, S. montevideo and S. muenchen each 2 strains, S. typhymurium, S. mbandaka, S. menden and S. hadar were 1 strain each, and 4 strains were not confirmed serovar. Seventy two strains of Salmonella isolates were tested for their susceptibility to 13 antimicrobial drugs by use of disc diffusion test. About 57% of the isolates were resistant to nalidixic acid(NA), 38.9% to ampicillin(AM), 34.7% to streptomycin (SM), 27.8% each to carbenicillin(CB) and tetracycline(TE), 18.1% to kanamycin(KA), and strains resistant to sulfamethoxazole/trimethoprim (ST) and cephalothin (CF) was less than 10%. The most frequent multiple resistant pattern was resistance to AM, CB, KA, SM, TE and NA(26.4%).
성명숙,김진현,김기석,Sung, Myung-Suk,Kim, Jin-Hyun,Kim, Ki-Seuk 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.3
Among 203 avian pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis in korea, 14 isolates were selected from total 68 isolates transferred R plasmid and classified into 5 groups on the basis of antimicrobial minimal inhibitory concentration (MIC) pattern, farm source and O serotype. An association between clonal origin and R plasmid of them was investigated by R plasmid profile, restriction endonuclease analysis and pulsed-field gel electrophoresis (PFGE). The strains that showed the same or very similar antimicrobial MIC pattern, but different farm source and O serotype, revealed different PFGE pattern, which seemed to be different clonal origin. And the strains that showed the same MIC pattern and O serotype, revealed different PFGE pattern, seemed to be originated from different clone. Also the strains showing the same MIC pattern and farm source, but different O serotype, revealed to be different clonal origin. The strains that showed the same or similar MIC pattern, farm source, and O serotype, revealed identical or similar PFGE pattern, which seemed to belong to be one clone. Meanwhile, horizontal transfer of R plasmid seems to be common in APEC with regardless of O serotype and clone of the strains. These results indicate that rapid and accurate epidemiological survey of APEC can be possible by the combination of O serotyping, plasmid profiling and PFGE analysis following the classification of them into groups of antimicrobial drug resistance pattern.
성명숙 中央醫學社 1993 中央醫學 Vol.58 No.11
The purpose of this study was to build a substantive theory about experience of selfmanagement for middle-aged man in Seoul. The qualitative research method have been used grounded theory. The interviewees were two middle-aged man. The data were collected through in-dept interviews by the investigator over a period of one month from 1 June to 30 June, 1993. The data were analyzed simultaneously by a constant comparative method in which new data are continuously coded into categories and properties according to Strauss and Corbin's methodology. The 61 codes were identified from the transcript of raw data and then 61 codes were identified to 12 categories. The 12 concepts which were found as a result of grounded data analyzing were, objectivity, regularity, rightness, balance, maintenance, conservation, endeavor, prevention, action, integration of body and mind, clarification of mind and physical health. This study enhances the nurse's understanding of the self-management of Korean middle-aged man. The findings contribute to nursing knowledge development about the self-management of middle-aged man.
성명숙,김진현,하종수,조재근,설성용,김기석,Sung, Myung-Suk,Kim, Jin-Hyun,Ha, Jong-Su,Cho, Jae-Keun,Seol, Sung-Yong,Kim, Ki-Seuk 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.2
This study was conducted to investigate biochemical properties and O group serotypes of pathogenic 203 Escherichia (E.) coli isolates from poultry with collibacillosis in Korea during the period from April 2003 to December 2005. Biochemical and fermentative properties of 203 isolates of E. coli tested were in accordance with Cowan and Steel's classification standard. One hundred and forty one isolates (69.5%) could be classified into a total of 20 O serotypes. Among them, the predominant O groups were O78 (32.5%), O88 (7.8%), O15 (6.8%), O141 (6.4%), and O158 (3.0%) in decreased order. Other infrequently encountered serogroups included : O8 (2%), O161 (2%), O20 (1.5%), O125 (1.5%), O2 (1%). And O6, O18, O24, O46, O76, O109, O119, O138, O139 and O148 had a frequency of 0.5%, respectively. Sixty two isolates (30.5%) were non-typeable with standard 173 O antisera used in this study.