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Apoptosis유발물질의 세포독성에 대한 Iysosomal stabilizer의 보호효과
석대은,명평근 충남대학교 약학대학 의약품개발연구소 1996 藥學論文集 Vol.12 No.-
Protective action of lysosomal stabilizer against cytotoxicity of apoptosis-inducers such as dexamethasone or doxorubicin in L 1210 cells was examined. Pepstatin as an inhibitor of lysosomal protease, more efficient than leupeptin, raised the viability of cells exposed to dexamethazone or doxorubicin form 55 % to 67-76 %. It also showed some protection against cytotoxicity of shikonin, an inhibitor of topoisomerase-Ⅰ by enhancing the viability from 50.3 % to 78 %. Gentamycin, an inhibitor of lysosomal phospholipase, increased the viability (55%) of cells exposed to dexamethaslne or doxorubicin to 66 % and 60 %, respectively. Meanwhile, dibucaine as an aromatic lysosomal stabilizer failed to prevent the cytotoxicity of dexamethazone. Thus, since inhibitors of lysosomal hydrolases expressed a partial protection against the cytotoxicity of apoptosis-inducers, it is suggested that cytotoxicity of apoptosis-inducers might be partially ascribed to the activation of lysosomal hydrolases.
Soybean Lipoxygenase-Catalyzed Conversion of Arachidonic Acid into Lipoxins
석대은,피택산,정창희,정윤수,강정부,Sok, Dai-Eun,Phi, Taek-San,Jung, Chang-Hee,Chung, Yun-Su,Kang, Jung-Bu 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.2
아라키돈산에 대두 lipoxygenase를 작용시켰을때, 300-301 nm에서 강한 흡수를 나타내는 극성 물질들이 형성되었다. 이 물질들을 UV스펙트럼, HPLC, GC/MS에 의한 분석 결과, lipoxin A와 lipoxin B 이성체로 확인되었다. 이 방법은 수백 마이크로그람의 liposin A 및 B 이성체를 얻는데 편리한 것으로, lipoxins의 생합성과 생리적 활성을 연구하는데 유용할 것으로 사료된다. The exposure of arachidonic acid to soybean lipoxygenase led to the formation of polar products showing intense absorption at 300-301 nm. These products were found to be lipoxin A and B isomers, based on UV spectrum, HPLC and GC/MS analyses. This method, convenient to prepare hundreds of micrograms of lipoxin A and B isomers, would be useful for the study on the biosynthesis and the biological activity of lipoxins.
5 , 15 - diHETE 의 주요 생합성 경로 : 아리키돈산으로부터 15 - HPETE를 거쳐 5 , 15 - diHETE로의 전환과정
석대은,찰스죤시 ( Dai Eun Sok,Charles J . Sih ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4
In both RBL-1 cells and human leukocytes, the calcium ionophore A 23187 enhanced the conversion of 15(S)-hydroxy-5,8,11-cis-13-trans-icosatetraenoic acid (15-HETE) to 5,15-diHETE by Ca^(++)-dependent 5-lipoxygenase (5-LPO). Interestingly, the amount of 5,15-diHETE formed from the incubation of 15(S)-hydroperoxy-5,8,11-cis-13-trans-icosatetraenoic acid (15-HPETE) with RBL-1 cells was similar to that generated from the incubation of 15-HETE with RBL-1 cells in the presence of A23187, suggesting that the 5-LPO-catalyzed conversion of 15-HPETE into 5,15-diHETE is independent of calcium ion. As a precursorial substrate for transformation to 5,15-diHETE in human leukocytes, 15-HPETE was found to be three times more efficient than 5-HPETE (5-hydroperoxy-6-trans-8,11,14-cis-icosatetraenoic acid). When 15-HETE, a strong inhibitor of lipoxygenation of arachidonic acid at C-5 or C-12, was added to the leukocytes suspension containing arachidonic acid and A 23187, the formation of 5,15-diHETE from arachidonic acid was increased to a great extent ($gt;3 times). Thus, the formation of 5,15-diHETE from arachidonic acid via 15-HPETE, which is Ca^(++)-independent and insensitive to 15-HETE, is supposed to be the more favorable pathway for the biosynthesis of 5,15-diHETE in the intact cells.
Simple Isolation of DNA from the Tissue Homogenate by Acridinium Affinity Chromatography
석대은,정창희,김윤배,정윤수,Sok, Dai-Eun,Jung, Chang-Hee,Kim, Yun-Bae,Chung, Yun-Su Korean Society for Biochemistry and Molecular Biol 1990 한국생화학회지 Vol.23 No.3
고염농도(1.4 M NaCl)의 어류 근조직 균질액을 아크리디늄 친화성 컬럼에 흡착시킨 후 2M $MgCl_2$로 용출시켜 고순도의 DNA ($A_{260}/A_{280}$?2.0)를 얻었다. 이 컬럼에 흡착된 DNA는 0.5M $MgCl_2$로도 용출되었으나 2M NaCl로는 용출되지 않았다. 한편, DNase-I 처리로 친화성 컬럼상의 DNA의 크로마토그래피 유형이 달라졌으며, 이 친화성 컬럼은 단일 및 이중 나선형 DNA 모두에 좋은 결합 친화성을 가진 것으로 여겨진다. 따라서, 이 방법은 고이온강도에서 생조직 균질액으로부터 DNA를 분리하는데 적합할 것이다. The homogenate of fish muscle tissue in high salt concentration (1.4 M NaCl) was applied on the acridinium affinity column, and the elution of the column with 2 M $MgCl_2$ produced the material, which turned out to be a highly pure DNA ($A_{260}/A_{280}$?2.0). The DNA bound on the column was desorbed easily with 0.5 M $MgCl_2$, but not even with 2 M NaCl. Separately, the treatment with DNase-I altered the chromatographic profile of DNA on the affinity column, and the affinity column seems to express good binding affinity toward both single and double stranded DNAs. This method would be suitable for the separation of DNA from crude tissue homogenates in high ionic strength.
대두 lipoxygenase 에 의한 아라키돈산으로부터 lipoxins 으로서 전환
석대은,피택산,정창희,정윤수,강정부 ( Dai Eun Sok,Taek San Phi,Chang Hee Jung,Yun Su Chung,Jung Bu Kang ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.2
The exposure of arachidonic acid to soybean lipoxygenase led to the formation of polar products showing intense absorption at 300-301 nm. These products were found to be lipoxin A and B isomers, based on UV spectrum, HPLC and GC/MS analyses. This method, convenient to prepare hundreds of micrograms of lipoxin A and B isomers, would be useful for the study on the biosynthesis and the biological activity of lipoxins.