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      • 유아기 자녀를 둔 아버지 교육 프로그램에 관한 조사연구

        서현아 慶星大學校 1997 論文集 Vol.18 No.2

        The purpose of this study is to provide basic resources for the development of effective father education program by analyzing the current father education plan undergoing in kindergartens. In order to achieve such a purpose, the writer collected and analyzed the 1996 program of 28 kindergartens in Busan. The material has been verified by percentage of frequency. The conclusions drawn from the study are as follow. 1. Period of practice The father education program was held the highest in November with 44.83% followed by in June with 27.59%. 2. Purpose The purpose that was layed out in the father education plan was the purpose related to the activity of the day with 33.33%, having pleasant time with children with 33.33%. 3. Method The father's class-participation was the highest with 50%. 4. Contest of education ① Subject Subjects related to science was the highest with 33.33%. ② Content of small group activity Art activity was the highest with 41.79% in small group activities. ③ Content of large group participation activity Song and dance was the highest with 38.49%. ④ Content of large group observing activity Story telling was the highest with 39.29%. 5. Schedule ① Starting time The starting time for father education program was 6 P.M. or 6:30 P.M. for weekdays, 2 P.M. for Saturday and 9 A.M. or 9:30 A.M. for Sunday. ② Total time From 1 hour and 30 minutes to 2 hours was the highest with 35.71%. ③ Precedure The precedure of planing - small group activity-large group activity-evaluation was the highest with 23.26%.

      • 가정탁아제의 효율적인 운영을 위한 기초연구

        서현아 慶星大學校 1993 論文集 Vol.14 No.1

        The porpose of this study is to foresee and prevent the problems which are caused by lack of motherhood through introducing family day care programs. For this purpose the following questions are discussed in the study 1.To define of family day care 2.To introduce the family day care system of Korea 3.To introduce the family day care system of U.S.A. 4.To make suggestion to improve family day care that is appropriate to Korean situation The aim of this was to introduce and set out the more effective family day care system in this country as early as possible by comparing the system in U.S.A. As a result, the following suggestions are obtained in conclusion 1.It is prerequisitive to legalize the system before planning and performing family day care program randomly 2.Legislation is urgently needed to attain qualitative improvement to the increase of number of family day care home. 3.It is necessary to unify the administrative system of early childhood education which is presently split into three or four ministries. 4.It is essential to establish the special research institute of Early Childhood Education and to develop the program. 5.It is necessary to train family day care givers systematically and repeatedly 6.Training program and institutions for teacher education must be establish as soon as possible

      • Shigella flexneri ATCC 29903를 이용한 ERIC-과 REP-PCR 반응 구성성분 농도의 최적화

        서현아,김근성 중앙대학교 유전공학연구소 2001 遺傳工學硏究論集 Vol.13 No.1

        We attempted to optimize the concentrations of PCR components such as MgCl2, Primers, dNTPs and template DNA to produce fingerpriting patterns of the ERIC- and REP-PCR products for Shigella flexneri ATCC 29903, The ERIC-PCR reactions resulted in a total number of 6 to 9 fragments and the REP-PCR reactions a total number of 10 to 14 fragments. These Phenomena can be explained by the hypothesis that more copies of REP sequences are present on bacterial chromosomes than those of ERIC sequences are. ERIC-PCR reactions generated two major bands of 0.7kb and 1.2kb DNA fragments. They also had the number of PCR products and their band intensity increased as the concentrations of the above four PCR components were increased REP-PCR reactions generated multiple PCR Products between 0.6kb and 4.1kb in size including three major bands of 0.6kb, 0.7kb and 2.2kb DNA fragments. Their band intensity was also increased as the concentrations of the above four PCR components were increased. The PCR reaction cocktails containing 2.0mM MgCl2, 2.0μM primers, 200μM dNTPs and 1.0㎕ template DNA, respectively, yielded the maximum number of PCR products. As the above four components were added more than the concentrations mentioned above in PCR reaction cocktails, the number of PCR products was not increased and the overall fingerprinting patterns remained unchanged. Therefore, a PCR reaction cocktail containing 2.0mM MgCl2, 2.0μM primers, 200μM dNTPs and 1.0㎕ template DNA can be suggested to generate the most optimized PCR fingerprinting patterns .

      • Staphylococcus aureus KN556를 이용한 ERIC-과 REP-PCR 반응 구성성분 농도의 최적화

        서현아,김영준,김근성 中央大學校 遺傳工學硏究所 2002 遺傳工學硏究論集 Vol.15 No.1

        본 실험은 Staphylococcus aureus KN556의 ERIC과 REP-PCR 산물의 fingerprinting pattern의 파악과 PCR 구성성분의 농도의 최적화를 목적으로 실시하였다. 실험결과 ERIC-PCR은 증폭된 단편수가 4∼10개 정도로 나타냈고, REP-PCR은 전체 단편수가 5∼14개를 나타내었다. 이러한 현상은 ERIC sequence에 비해 REP sequence가 세균의 염색체 상에 빈도가 더 높게 분포한다는 이론과 일치하였다. ERIC-PCR의 경우, 0.8과 1.2kb를 중심으로 작은 분자량과 큰 분자량의 범위로 점차적으로 확대되어 각 변수들의 농도의 증가에 따라 전체단편의 수와 단편들의 강도가 증가하였다. REP-PCR은 최소분자량 0.25kb에서 최대분자량 3.5kb 정도 크기의 증폭된 단편들이 각 농도에 따라 강도의 차이를 나타내며 증가되는 것이 관찰되었다. 각 성분변수별로 MgCl_2는 2.0mM, primer는 2.0μM, dNTPs는 200μM, DNA는 1.0㎕에서 각각 최대 단편수를 나타냈고, 그 이상의 농도 적용 시에도 동일한 단편의 수를 유지하였으며 각 성분농도 변수 PVR 결과 나타나는 DNA fingerprinting pattern이 동일하게 나타나서 결과적으로 PCR 성분별 최적농도는 MgCl_2는 2.0mM, primer는 2.0μM, dNTPs는 200μM, DNA는 1.0㎕로 결론 지을 수 있었다. We attempted to optimize the concentrations of PCR components such as MgCl_2, primers, dNTPs and template DNA to produce fingerprinting patterns of the ERIC- and REP-PCR products for Staphylococcus aureus KN556. The ERIC-PCR reactions resulted in a total number of 4 to 10 DNA fragments and the REP-PCR reactions a total number of 5 to 14 DNA fragments. These phenomena can be explained by the hypothesis that more copies of REP sequences are present on bacterial chromosomes than those of ERIC sequences are. ERIC-PCR reactions generated two major bands of 0.8kb and 1.2kb DNA fragments. They also had the number of PCR products and their band intensity increased as the concentrations of the above four PCR components were increased. REP-PCR reactions generated multiple PCR products between 0.25kb and 3.5kb in size including three major bands of 0.7kb and 0.95kb DNA fragments. Their band intensity was also increased as the concentrations of the above four PCR components were increased. The PCR reaction cocktails containing 2.0mM MgCl_2, 2.0μM primers, 200μM dNTPs and 1.0㎕ template DNA, respectively, yielded the maximum number of PCR products. As the above four components were added more than the concentrations mentioned above in PCR reaction cocktails, the number of PCR products was not increased and the overall fingerprinting patterns remained unchanged. Therefore, a PCR reaction cocktail containing 2.0mM MgCl_2, 2.0μM primers, 200μM dNTPs and 1.0㎕ template DNA can be suggested to generate the most optimized PCR fingerprinting patterns.

      • 장애유아를 위한 교육내용에 관한 일 연구

        서현아 慶星大學校 1997 論文集 Vol.18 No.1

        The purpose of this study is to investigate which educational content is appropriate for preschoolers with handicaps. In order to achieve such a purpose, the writer surveyed the study was primarily dependent upon literature as a descriptive and analytic approach for its methodology. The conclusions drawn from the study are as follows. The educational goals and guidelines for organizing space for preschoolers with handicaps are examined. The educational content for preschoolers with handicaps contains intellectual skills, communication skills, social skills, motor skills, self-help skills, evaluation, program(ABACUS),transition

      • 정상유아 및 정신지체유아의 사물놀이행동에 영향을 미치는 변인 연구

        서현아 慶星大學校 1994 論文集 Vol.15 No.1

        The purpose of this study was to determine the effects of the variables which are play material, gender difference affecting object play behavior exhibited by mentally retarded young children, and nonhandicapped young children. In order to achieve such a purpose, the writer surveyed the study was primarily dependent upon literature as a descriptive and analytic approach for its methodology. The conclusions drawn from the study are as follows. These studies on effects of toys imply that materials made available to children may affect the behaviors they engage in while interacting with play objects and with other children. Toy variables that may affect children's play behavior with blocks and dolls appearing to encourage social interaction and art activities appearing to inhibit it. Art materials appear to enhance functional and constructive aspects of play. Although the results are inconsistent with findings from several researches, gender differences have significant effect on toy preference and type of play behavior. In age difference variable, it is clear that increases in developmental age have been positively associated with increases in play sophistication.

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